TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.hbd-sbc.hr/kongres2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6564
AB  - Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
PB  - Zagreb: Hrvatsko biološko društvo
C3  - Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
T1  - Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
SP  - 231
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6564
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Karan, Jelena and Ivelja, Ivana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.",
publisher = "Zagreb: Hrvatsko biološko društvo",
journal = "Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska",
title = "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes",
pages = "231-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6564"
}

Marković Filipović, J., Kojić, D., Miler, M., Karan, J., Ivelja, I.,& Matavulj, M.. (2022). Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
Zagreb: Hrvatsko biološko društvo., 231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564

Marković Filipović J, Kojić D, Miler M, Karan J, Ivelja I, Matavulj M. Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. 2022;:231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Karan, Jelena, Ivelja, Ivana, Matavulj, Milica, "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes" in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska (2022):231-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

TY  - JOUR
AU  - Marković-Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Andrejić Višnjić, Bojana
AU  - Milošević, Verica
AU  - Cukuranovic Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Matavulj, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5480
AB  - Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.
T2  - International Journal of Morphology
T1  - Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study
IS  - 6
VL  - 40
DO  - 10.4067/s0717-95022022000601618
SP  - 1618
EP  - 1623
ER  - 

@article{
author = "Marković-Filipović, Jelena and Miler, Marko and Kojić, Danijela and Andrejić Višnjić, Bojana and Milošević, Verica and Cukuranovic Kokoris, Jovana and Đorđević, Miodrag and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.",
journal = "International Journal of Morphology",
title = "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study",
number = "6",
volume = "40",
doi = "10.4067/s0717-95022022000601618",
pages = "1618-1623"
}

Marković-Filipović, J., Miler, M., Kojić, D., Andrejić Višnjić, B., Milošević, V., Cukuranovic Kokoris, J., Đorđević, M.,& Matavulj, M.. (2022). Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology, 40(6), 1618-1623.
https://doi.org/10.4067/s0717-95022022000601618

Marković-Filipović J, Miler M, Kojić D, Andrejić Višnjić B, Milošević V, Cukuranovic Kokoris J, Đorđević M, Matavulj M. Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology. 2022;40(6):1618-1623.
doi:10.4067/s0717-95022022000601618 .

Marković-Filipović, Jelena, Miler, Marko, Kojić, Danijela, Andrejić Višnjić, Bojana, Milošević, Verica, Cukuranovic Kokoris, Jovana, Đorđević, Miodrag, Matavulj, Milica, "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study" in International Journal of Morphology, 40, no. 6 (2022):1618-1623,
https://doi.org/10.4067/s0717-95022022000601618 . .

TY  - JOUR
AU  - Marković Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Čukuranović Kokoris, Jovana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/11/6062
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5007
AB  - Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.
IS  - 11
VL  - 23
DO  - 10.3390/ijms23116062
SP  - 6062
ER  - 

@article{
author = "Marković Filipović, Jelena and Miler, Marko and Kojić, Danijela and Karan, Jelena and Ivelja, Ivana and Čukuranović Kokoris, Jovana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.",
number = "11",
volume = "23",
doi = "10.3390/ijms23116062",
pages = "6062"
}

Marković Filipović, J., Miler, M., Kojić, D., Karan, J., Ivelja, I., Čukuranović Kokoris, J.,& Matavulj, M.. (2022). Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences
Basel: MDPI., 23(11), 6062.
https://doi.org/10.3390/ijms23116062

Marković Filipović J, Miler M, Kojić D, Karan J, Ivelja I, Čukuranović Kokoris J, Matavulj M. Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences. 2022;23(11):6062.
doi:10.3390/ijms23116062 .

Marković Filipović, Jelena, Miler, Marko, Kojić, Danijela, Karan, Jelena, Ivelja, Ivana, Čukuranović Kokoris, Jovana, Matavulj, Milica, "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes." in International Journal of Molecular Sciences, 23, no. 11 (2022):6062,
https://doi.org/10.3390/ijms23116062 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6578
AB  - INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Acrylamide treatment affects oxidative stress parameters in rat hepatocytes
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6578
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6578"
}

Marković Filipović, J., Kojić, D., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578

Marković Filipović J, Kojić D, Miler M, Milošević V, Matavulj M. Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Čelić, Tatjana
AU  - Pihler, Ivan
AU  - Blagojević, Duško
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417&tlng=en
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4699
AB  - The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.
PB  - Parana, Brazil: Technology Institute of Paraná
T2  - Brazilian Archives of Biology and Technology
T1  - Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut
VL  - 64
DO  - 10.1590/1678-4324-2021200773
SP  - e21200773
ER  - 

@article{
author = "Vukašinović, Elvira and Purać, Jelena and Kojić, Danijela and Čelić, Tatjana and Pihler, Ivan and Blagojević, Duško",
year = "2021",
abstract = "The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.",
publisher = "Parana, Brazil: Technology Institute of Paraná",
journal = "Brazilian Archives of Biology and Technology",
title = "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut",
volume = "64",
doi = "10.1590/1678-4324-2021200773",
pages = "e21200773"
}

Vukašinović, E., Purać, J., Kojić, D., Čelić, T., Pihler, I.,& Blagojević, D.. (2021). Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology
Parana, Brazil: Technology Institute of Paraná., 64, e21200773.
https://doi.org/10.1590/1678-4324-2021200773

Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology. 2021;64:e21200773.
doi:10.1590/1678-4324-2021200773 .

Vukašinović, Elvira, Purać, Jelena, Kojić, Danijela, Čelić, Tatjana, Pihler, Ivan, Blagojević, Duško, "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut" in Brazilian Archives of Biology and Technology, 64 (2021):e21200773,
https://doi.org/10.1590/1678-4324-2021200773 . .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32777467
UR  - https://radar.ibiss.bg.ac.rs/123456789/3861
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
SP  - 108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852",
pages = "108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević D, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško, Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph." in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - JOUR
AU  - Uzelac, Iva
AU  - Avramov, Miloš
AU  - Čelić, Tatjana
AU  - Vukašinović, Elvira
AU  - Gošić-Dondo, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Blagojević, Duško
AU  - Popović, Željko D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3706
AB  - The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
PB  - Nature Research
T2  - Scientific Reports
T1  - Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-65926-w
SP  - 1
EP  - 11
ER  - 

@article{
author = "Uzelac, Iva and Avramov, Miloš and Čelić, Tatjana and Vukašinović, Elvira and Gošić-Dondo, Snežana and Purać, Jelena and Kojić, Danijela and Blagojević, Duško and Popović, Željko D.",
year = "2020",
abstract = "The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-65926-w",
pages = "1-11"
}

Uzelac, I., Avramov, M., Čelić, T., Vukašinović, E., Gošić-Dondo, S., Purać, J., Kojić, D., Blagojević, D.,& Popović, Ž. D.. (2020). Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports
Nature Research., 10(1), 1-11.
https://doi.org/10.1038/s41598-020-65926-w

Uzelac I, Avramov M, Čelić T, Vukašinović E, Gošić-Dondo S, Purać J, Kojić D, Blagojević D, Popović ŽD. Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports. 2020;10(1):1-11.
doi:10.1038/s41598-020-65926-w .

Uzelac, Iva, Avramov, Miloš, Čelić, Tatjana, Vukašinović, Elvira, Gošić-Dondo, Snežana, Purać, Jelena, Kojić, Danijela, Blagojević, Duško, Popović, Željko D., "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)" in Scientific Reports, 10, no. 1 (2020):1-11,
https://doi.org/10.1038/s41598-020-65926-w . .

TY  - JOUR
AU  - Vukašinović, Elvira L.
AU  - Čelić, Tatjana V.
AU  - Kojić, Danijela
AU  - Franeta, Filip
AU  - Milić, Stanko
AU  - Ninkov, Jordana
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0045653519326153?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3536
AB  - In this study the effect of long term exposure to cadmium (Cd) on Ostrinia nubilalis larval growth, development, survival rate and oxidative status was analyzed. Newly hatched first instar - L1 larvae were reared on a Cd contaminated diet until the larvae reached the final, fifth instar - L5 or developed into pupae. In total, six experimental groups, five treatments (concentrations of Cd in fresh diet: Cd I: 0.73, Cd II: 3.70, Cd III: 6.85, Cd IV: 41.71 and Cd V: 77.53 mg kg-1) and a control group (C) were set up. The results of the experiment showed that exposure to higher concentrations of Cd (41.71 and 77.53 mg kg-1) had a significant influence on development and redox status of O. nubilalis larvae: (1) the development rate was strongly reduced resulting in a prolonged pupation time; (2) the survival rate of larvae was prominently lower; (3) bioaccumulation factor (measured in pupae) was reduced which indicated that larvae could accumulate Cd to a certain level; (4) the level of the lipid peroxidation was significantly higher, which points to oxidative damage; (5) the expression of Mtn was significantly up-regulated while Cat and GPx genes down-regulated. In conclusion, long term exposure to dietary Cd in a concentration of 41.7 mg kg-1 and higher, induced oxidative stress and slowed down growth and development of O. nubilalis larvae.
T2  - Chemosphere
T1  - The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.
VL  - 243
DO  - 10.1016/j.chemosphere.2019.125375
SP  - 125375
ER  - 

@article{
author = "Vukašinović, Elvira L. and Čelić, Tatjana V. and Kojić, Danijela and Franeta, Filip and Milić, Stanko and Ninkov, Jordana and Blagojević, Duško and Purać, Jelena",
year = "2020",
abstract = "In this study the effect of long term exposure to cadmium (Cd) on Ostrinia nubilalis larval growth, development, survival rate and oxidative status was analyzed. Newly hatched first instar - L1 larvae were reared on a Cd contaminated diet until the larvae reached the final, fifth instar - L5 or developed into pupae. In total, six experimental groups, five treatments (concentrations of Cd in fresh diet: Cd I: 0.73, Cd II: 3.70, Cd III: 6.85, Cd IV: 41.71 and Cd V: 77.53 mg kg-1) and a control group (C) were set up. The results of the experiment showed that exposure to higher concentrations of Cd (41.71 and 77.53 mg kg-1) had a significant influence on development and redox status of O. nubilalis larvae: (1) the development rate was strongly reduced resulting in a prolonged pupation time; (2) the survival rate of larvae was prominently lower; (3) bioaccumulation factor (measured in pupae) was reduced which indicated that larvae could accumulate Cd to a certain level; (4) the level of the lipid peroxidation was significantly higher, which points to oxidative damage; (5) the expression of Mtn was significantly up-regulated while Cat and GPx genes down-regulated. In conclusion, long term exposure to dietary Cd in a concentration of 41.7 mg kg-1 and higher, induced oxidative stress and slowed down growth and development of O. nubilalis larvae.",
journal = "Chemosphere",
title = "The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.",
volume = "243",
doi = "10.1016/j.chemosphere.2019.125375",
pages = "125375"
}

Vukašinović, E. L., Čelić, T. V., Kojić, D., Franeta, F., Milić, S., Ninkov, J., Blagojević, D.,& Purać, J.. (2020). The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.. in Chemosphere, 243, 125375.
https://doi.org/10.1016/j.chemosphere.2019.125375

Vukašinović EL, Čelić TV, Kojić D, Franeta F, Milić S, Ninkov J, Blagojević D, Purać J. The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status.. in Chemosphere. 2020;243:125375.
doi:10.1016/j.chemosphere.2019.125375 .

Vukašinović, Elvira L., Čelić, Tatjana V., Kojić, Danijela, Franeta, Filip, Milić, Stanko, Ninkov, Jordana, Blagojević, Duško, Purać, Jelena, "The effect of long term exposure to cadmium on Ostrinia nubilalis growth, development, survival rate and oxidative status." in Chemosphere, 243 (2020):125375,
https://doi.org/10.1016/j.chemosphere.2019.125375 . .

TY  - JOUR
AU  - Nikolić, Tatjana
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-018-3950-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3236
AB  - In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.
T2  - Environmental Science and Pollution Research
T1  - Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.
DO  - 10.1007/s11356-018-3950-6
ER  - 

@article{
author = "Nikolić, Tatjana and Kojić, Danijela and Orčić, Snežana and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2019",
abstract = "In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.",
journal = "Environmental Science and Pollution Research",
title = "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.",
doi = "10.1007/s11356-018-3950-6"
}

Nikolić, T., Kojić, D., Orčić, S., Vukašinović, E., Blagojević, D.,& Purać, J.. (2019). Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-018-3950-6

Nikolić T, Kojić D, Orčić S, Vukašinović E, Blagojević D, Purać J. Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-018-3950-6 .

Nikolić, Tatjana, Kojić, Danijela, Orčić, Snežana, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-018-3950-6 . .

TY  - JOUR
AU  - Purać, Jelena
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Ćelić, Anđelka S.
AU  - Plavša, Jovana J.
AU  - Blagojević, Duško
AU  - Petri, Edward T.
PY  - 2019
UR  - http://doi.wiley.com/10.1111/mec.14984
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3279
AB  - Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.
T2  - Molecular Ecology
T1  - Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.
IS  - 4
VL  - 28
DO  - 10.1111/mec.14984
SP  - 731
EP  - 745
ER  - 

@article{
author = "Purać, Jelena and Nikolić, Tatjana V. and Kojić, Danijela and Ćelić, Anđelka S. and Plavša, Jovana J. and Blagojević, Duško and Petri, Edward T.",
year = "2019",
abstract = "Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.",
journal = "Molecular Ecology",
title = "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.",
number = "4",
volume = "28",
doi = "10.1111/mec.14984",
pages = "731-745"
}

Purać, J., Nikolić, T. V., Kojić, D., Ćelić, A. S., Plavša, J. J., Blagojević, D.,& Petri, E. T.. (2019). Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology, 28(4), 731-745.
https://doi.org/10.1111/mec.14984

Purać J, Nikolić TV, Kojić D, Ćelić AS, Plavša JJ, Blagojević D, Petri ET. Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology. 2019;28(4):731-745.
doi:10.1111/mec.14984 .

Purać, Jelena, Nikolić, Tatjana V., Kojić, Danijela, Ćelić, Anđelka S., Plavša, Jovana J., Blagojević, Duško, Petri, Edward T., "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure." in Molecular Ecology, 28, no. 4 (2019):731-745,
https://doi.org/10.1111/mec.14984 . .

TY  - JOUR
AU  - Vukšinović, Elvira L.
AU  - Pond, David W.
AU  - Grubor-Lajšić, Gordana
AU  - Worland, M. Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko D.
AU  - Blagojević, Duško
PY  - 2018
UR  - http://link.springer.com/10.1007/s00360-017-1110-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2777
AB  - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
T2  - Journal of Comparative Physiology B
T1  - Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls
VL  - 188
DO  - 10.1007/s00360-017-1110-9
SP  - 27
EP  - 36
ER  - 

@article{
author = "Vukšinović, Elvira L. and Pond, David W. and Grubor-Lajšić, Gordana and Worland, M. Roger and Kojić, Danijela and Purać, Jelena and Popović, Željko D. and Blagojević, Duško",
year = "2018",
abstract = "Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.",
journal = "Journal of Comparative Physiology B",
title = "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls",
volume = "188",
doi = "10.1007/s00360-017-1110-9",
pages = "27-36"
}

Vukšinović, E. L., Pond, D. W., Grubor-Lajšić, G., Worland, M. R., Kojić, D., Purać, J., Popović, Ž. D.,& Blagojević, D.. (2018). Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B, 188, 27-36.
https://doi.org/10.1007/s00360-017-1110-9

Vukšinović EL, Pond DW, Grubor-Lajšić G, Worland MR, Kojić D, Purać J, Popović ŽD, Blagojević D. Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B. 2018;188:27-36.
doi:10.1007/s00360-017-1110-9 .

Vukšinović, Elvira L., Pond, David W., Grubor-Lajšić, Gordana, Worland, M. Roger, Kojić, Danijela, Purać, Jelena, Popović, Željko D., Blagojević, Duško, "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls" in Journal of Comparative Physiology B, 188 (2018):27-36,
https://doi.org/10.1007/s00360-017-1110-9 . .

TY  - JOUR
AU  - Kojić, Danijela
AU  - Popović, Željko D.
AU  - Orčić, Dejan
AU  - Purać, Jelena
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira L.
AU  - Nikolić, Tatjana V.
AU  - Blagojević, Duško
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0022191018300921?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3116
AB  - The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.
T2  - Journal of insect physiology
T1  - The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).
VL  - 109
DO  - 10.1016/j.jinsphys.2018.07.007
SP  - 107
EP  - 113
ER  - 

@article{
author = "Kojić, Danijela and Popović, Željko D. and Orčić, Dejan and Purać, Jelena and Orčić, Snežana and Vukašinović, Elvira L. and Nikolić, Tatjana V. and Blagojević, Duško",
year = "2018",
abstract = "The European corn borer (ECB, Ostrinia nubilalis Hbn.) is a major pest in temperate regions of Europe and North America. Fifth instar ECB larvae enter diapause before winter and gradually develop cold hardiness. Here we investigated the combined influence of diapause phase and low temperature on sugar and polyol content in ECB larvae. Larvae in mid-diapause or diapause termination were acclimated at 5 °C, -3°C or -16 °C, and sugar and polyol content was measured using GC-MS. Control GC-MS measurements were conducted on untreated non-diapausing larvae. We detected differences in polyol (glycerol, sorbitol, myo-inositol) and sugar (trehalose, fructose, glucose) levels in diapausing versus non-diapausing larvae. Glycerol and trehalose were the most abundant of all analyzed cryoprotective compounds in diapausing larvae. Exposure of diapausing larvae to decreasing temperatures induced changes in polyol and sugar levels that depended on the phase of diapause. In mid-diapause larvae, decreasing temperatures induced a significant increase in glycerol and a decrease in sorbitol and myo-inositol. In larvae at diapause termination, polyol content was lower and less influenced by decreasing temperatures. In contrast, sugar levels were lower in larvae at mid-diapause versus diapause termination. Exposure of larvae to -16 °C induced a significant increase in the levels of all detected sugars. In particular, glucose levels were significantly higher in larvae at diapause termination following exposure to -16 °C. We propose that this shift toward sugar synthesis following low temperature exposure in larvae at diapause termination is a consequence of NADPH dependent polyol synthesis, and may be a mechanism for preservation of carbon reserves needed for post-diapause development.",
journal = "Journal of insect physiology",
title = "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).",
volume = "109",
doi = "10.1016/j.jinsphys.2018.07.007",
pages = "107-113"
}

Kojić, D., Popović, Ž. D., Orčić, D., Purać, J., Orčić, S., Vukašinović, E. L., Nikolić, T. V.,& Blagojević, D.. (2018). The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology, 109, 107-113.
https://doi.org/10.1016/j.jinsphys.2018.07.007

Kojić D, Popović ŽD, Orčić D, Purać J, Orčić S, Vukašinović EL, Nikolić TV, Blagojević D. The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.).. in Journal of insect physiology. 2018;109:107-113.
doi:10.1016/j.jinsphys.2018.07.007 .

Kojić, Danijela, Popović, Željko D., Orčić, Dejan, Purać, Jelena, Orčić, Snežana, Vukašinović, Elvira L., Nikolić, Tatjana V., Blagojević, Duško, "The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.)." in Journal of insect physiology, 109 (2018):107-113,
https://doi.org/10.1016/j.jinsphys.2018.07.007 . .

TY  - JOUR
AU  - Orčić, Snežana
AU  - Nikolić, Tatjana
AU  - Purać, Jelena
AU  - Šikoparija, Branko
AU  - Blagojević, Duško
AU  - Vukašinović, Elvira
AU  - Plavša, Nada
AU  - Stevanović, Jevrosima
AU  - Kojić, Danijela
PY  - 2017
UR  - http://doi.wiley.com/10.1111/eea.12633
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2964
AB  - The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.
T2  - Entomologia Experimentalis et Applicata
T1  - Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees
IS  - 2-3
VL  - 165
DO  - 10.1111/eea.12633
SP  - 120
EP  - 128
ER  - 

@article{
author = "Orčić, Snežana and Nikolić, Tatjana and Purać, Jelena and Šikoparija, Branko and Blagojević, Duško and Vukašinović, Elvira and Plavša, Nada and Stevanović, Jevrosima and Kojić, Danijela",
year = "2017",
abstract = "The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.",
journal = "Entomologia Experimentalis et Applicata",
title = "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees",
number = "2-3",
volume = "165",
doi = "10.1111/eea.12633",
pages = "120-128"
}

Orčić, S., Nikolić, T., Purać, J., Šikoparija, B., Blagojević, D., Vukašinović, E., Plavša, N., Stevanović, J.,& Kojić, D.. (2017). Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata, 165(2-3), 120-128.
https://doi.org/10.1111/eea.12633

Orčić S, Nikolić T, Purać J, Šikoparija B, Blagojević D, Vukašinović E, Plavša N, Stevanović J, Kojić D. Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata. 2017;165(2-3):120-128.
doi:10.1111/eea.12633 .

Orčić, Snežana, Nikolić, Tatjana, Purać, Jelena, Šikoparija, Branko, Blagojević, Duško, Vukašinović, Elvira, Plavša, Nada, Stevanović, Jevrosima, Kojić, Danijela, "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees" in Entomologia Experimentalis et Applicata, 165, no. 2-3 (2017):120-128,
https://doi.org/10.1111/eea.12633 . .

TY  - CHAP
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Petri, Edward
AU  - Popović, Željko D.
AU  - Grubor-Lajšić, Gordana
AU  - Blagojević, Duško
AU  - Raman, Chandrasekar
AU  - Goldsmith, Marian R.
AU  - Agunbiade, Tolulope A.
PY  - 2016
UR  - http://link.springer.com/10.1007/978-3-319-24244-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2610
AB  - In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.
PB  - Springer International Publishing
T2  - Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
T1  - Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach
DO  - 10.1007/978-3-319-24244-6_4
SP  - 89
EP  - 112
ER  - 

@inbook{
author = "Purać, Jelena and Kojić, Danijela and Petri, Edward and Popović, Željko D. and Grubor-Lajšić, Gordana and Blagojević, Duško and Raman, Chandrasekar and Goldsmith, Marian R. and Agunbiade, Tolulope A.",
year = "2016",
abstract = "In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.",
publisher = "Springer International Publishing",
journal = "Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2",
booktitle = "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach",
doi = "10.1007/978-3-319-24244-6_4",
pages = "89-112"
}

Purać, J., Kojić, D., Petri, E., Popović, Ž. D., Grubor-Lajšić, G., Blagojević, D., Raman, C., Goldsmith, M. R.,& Agunbiade, T. A.. (2016). Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
Springer International Publishing., 89-112.
https://doi.org/10.1007/978-3-319-24244-6_4

Purać J, Kojić D, Petri E, Popović ŽD, Grubor-Lajšić G, Blagojević D, Raman C, Goldsmith MR, Agunbiade TA. Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2. 2016;:89-112.
doi:10.1007/978-3-319-24244-6_4 .

Purać, Jelena, Kojić, Danijela, Petri, Edward, Popović, Željko D., Grubor-Lajšić, Gordana, Blagojević, Duško, Raman, Chandrasekar, Goldsmith, Marian R., Agunbiade, Tolulope A., "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach" in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2 (2016):89-112,
https://doi.org/10.1007/978-3-319-24244-6_4 . .

TY  - JOUR
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Batinić, Darko
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27580263
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0045653516311006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2611
AB  - In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.
T2  - Chemosphere
T1  - The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.
VL  - 164
DO  - 10.1016/j.chemosphere.2016.08.077
SP  - 98
EP  - 105
ER  - 

@article{
author = "Nikolić, Tatjana V. and Kojić, Danijela and Orčić, Snežana and Batinić, Darko and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2016",
abstract = "In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.",
journal = "Chemosphere",
title = "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.",
volume = "164",
doi = "10.1016/j.chemosphere.2016.08.077",
pages = "98-105"
}

Nikolić, T. V., Kojić, D., Orčić, S., Batinić, D., Vukašinović, E., Blagojević, D.,& Purać, J.. (2016). The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere, 164, 98-105.
https://doi.org/10.1016/j.chemosphere.2016.08.077

Nikolić TV, Kojić D, Orčić S, Batinić D, Vukašinović E, Blagojević D, Purać J. The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere. 2016;164:98-105.
doi:10.1016/j.chemosphere.2016.08.077 .

Nikolić, Tatjana V., Kojić, Danijela, Orčić, Snežana, Batinić, Darko, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions." in Chemosphere, 164 (2016):98-105,
https://doi.org/10.1016/j.chemosphere.2016.08.077 . .

TY  - JOUR
AU  - Grubor-Lajšić, Gordana
AU  - Petri, Edward
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko
AU  - Worland, Michael Roger
AU  - Clark, Melody S.
AU  - Mojović, Miloš
AU  - Blagojević, Duško
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6338
AB  - The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.
PB  - Hoboken: John Wiley and Sons
T2  - Archives of Insect Biochemistry and Physiology
T1  - Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)
IS  - 2
VL  - 82
DO  - 10.1002/arch.21073
SP  - 59
EP  - 70
ER  - 

@article{
author = "Grubor-Lajšić, Gordana and Petri, Edward and Kojić, Danijela and Purać, Jelena and Popović, Željko and Worland, Michael Roger and Clark, Melody S. and Mojović, Miloš and Blagojević, Duško",
year = "2013",
abstract = "The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)",
number = "2",
volume = "82",
doi = "10.1002/arch.21073",
pages = "59-70"
}

Grubor-Lajšić, G., Petri, E., Kojić, D., Purać, J., Popović, Ž., Worland, M. R., Clark, M. S., Mojović, M.,& Blagojević, D.. (2013). Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons., 82(2), 59-70.
https://doi.org/10.1002/arch.21073

Grubor-Lajšić G, Petri E, Kojić D, Purać J, Popović Ž, Worland MR, Clark MS, Mojović M, Blagojević D. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology. 2013;82(2):59-70.
doi:10.1002/arch.21073 .

Grubor-Lajšić, Gordana, Petri, Edward, Kojić, Danijela, Purać, Jelena, Popović, Željko, Worland, Michael Roger, Clark, Melody S., Mojović, Miloš, Blagojević, Duško, "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)" in Archives of Insect Biochemistry and Physiology, 82, no. 2 (2013):59-70,
https://doi.org/10.1002/arch.21073 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Pond, David
AU  - Worland, Michael Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6337
AB  - This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
T1  - Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
IS  - 4
VL  - 165
DO  - 10.1016/j.cbpb.2013.05.001
SP  - 219
EP  - 225
ER  - 

@article{
author = "Vukašinović, Elvira and Pond, David and Worland, Michael Roger and Kojić, Danijela and Purać, Jelena and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2013",
abstract = "This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology",
title = "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)",
number = "4",
volume = "165",
doi = "10.1016/j.cbpb.2013.05.001",
pages = "219-225"
}

Vukašinović, E., Pond, D., Worland, M. R., Kojić, D., Purać, J., Blagojević, D.,& Grubor-Lajšić, G.. (2013). Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Elsevier Inc.., 165(4), 219-225.
https://doi.org/10.1016/j.cbpb.2013.05.001

Vukašinović E, Pond D, Worland MR, Kojić D, Purać J, Blagojević D, Grubor-Lajšić G. Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2013;165(4):219-225.
doi:10.1016/j.cbpb.2013.05.001 .

Vukašinović, Elvira, Pond, David, Worland, Michael Roger, Kojić, Danijela, Purać, Jelena, Blagojević, Duško, Grubor-Lajšić, Gordana, "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)" in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 165, no. 4 (2013):219-225,
https://doi.org/10.1016/j.cbpb.2013.05.001 . .

TY  - JOUR
AU  - Popović, Ž.D.
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Pamer, Elvira L.
AU  - Worland, M.R.
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/282
AB  - The Arctic springtail Megaphorura arctica (Tullberg, 1876) employs a strategy known as cryoprotective dehydration to survive winter temperatures as low as -25°C. During cryoprotective dehydration, water is lost from the animal to ice in its surroundings as a result of the difference in vapour pressure between the animal's supercooled body fluids and ice (Worland et al., 1998; Holmstrup and Somme, 1998). This mechanism ensures that as the habitat temperature falls, the concentration of solutes remains high enough to prevent freezing (Holmstrup et al., 2002). In M. arctica, accumulation of trehalose, a cryo/anhydro protectant, occurs in parallel with dehydration. Recent studies have identified a number of genes and cellular processes involved in cryoprotective dehydration in M. arctica (Clark et al., 2007; Clark et al., 2009; Purać et al., 2011). One of them includes late embryogenesis abundant (LEA) proteins. This study, together with that of Bahrndorff et al. (2008), suggests that LEA proteins may be involved in protective dehydration in this species.
T2  - Archives of Biological Sciences
T1  - Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica
IS  - 3
VL  - 63
SP  - 681
EP  - 683
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_282
ER  - 

@article{
author = "Popović, Ž.D. and Purać, Jelena and Kojić, Danijela and Pamer, Elvira L. and Worland, M.R. and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2011, 2011",
abstract = "The Arctic springtail Megaphorura arctica (Tullberg, 1876) employs a strategy known as cryoprotective dehydration to survive winter temperatures as low as -25°C. During cryoprotective dehydration, water is lost from the animal to ice in its surroundings as a result of the difference in vapour pressure between the animal's supercooled body fluids and ice (Worland et al., 1998; Holmstrup and Somme, 1998). This mechanism ensures that as the habitat temperature falls, the concentration of solutes remains high enough to prevent freezing (Holmstrup et al., 2002). In M. arctica, accumulation of trehalose, a cryo/anhydro protectant, occurs in parallel with dehydration. Recent studies have identified a number of genes and cellular processes involved in cryoprotective dehydration in M. arctica (Clark et al., 2007; Clark et al., 2009; Purać et al., 2011). One of them includes late embryogenesis abundant (LEA) proteins. This study, together with that of Bahrndorff et al. (2008), suggests that LEA proteins may be involved in protective dehydration in this species.",
journal = "Archives of Biological Sciences",
title = "Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica",
number = "3",
volume = "63",
pages = "681-683",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_282"
}

Popović, Ž.D., Purać, J., Kojić, D., Pamer, E. L., Worland, M.R., Blagojević, D.,& Grubor-Lajšić, G.. (2011). Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica. in Archives of Biological Sciences, 63(3), 681-683.
https://hdl.handle.net/21.15107/rcub_ibiss_282

Popović Ž, Purać J, Kojić D, Pamer EL, Worland M, Blagojević D, Grubor-Lajšić G. Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica. in Archives of Biological Sciences. 2011;63(3):681-683.
https://hdl.handle.net/21.15107/rcub_ibiss_282 .

Popović, Ž.D., Purać, Jelena, Kojić, Danijela, Pamer, Elvira L., Worland, M.R., Blagojević, Duško, Grubor-Lajšić, Gordana, "Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica" in Archives of Biological Sciences, 63, no. 3 (2011):681-683,
https://hdl.handle.net/21.15107/rcub_ibiss_282 .