TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Jankovic, Teodora
AU  - Pljevljakusic, Dejan
AU  - Ninković, Slavica
AU  - Smigocki, Ann
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1952
AB  - The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0737-z
SP  - 667
EP  - 679
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Jankovic, Teodora and Pljevljakusic, Dejan and Ninković, Slavica and Smigocki, Ann and Vinterhalter, Dragan",
year = "2015",
abstract = "The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0737-z",
pages = "667-679"
}

Vinterhalter, B., Krstić Milošević, D., Jankovic, T., Pljevljakusic, D., Ninković, S., Smigocki, A.,& Vinterhalter, D.. (2015). Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture, 121(3), 667-679.
https://doi.org/10.1007/s11240-015-0737-z

Vinterhalter B, Krstić Milošević D, Jankovic T, Pljevljakusic D, Ninković S, Smigocki A, Vinterhalter D. Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture. 2015;121(3):667-679.
doi:10.1007/s11240-015-0737-z .

Vinterhalter, Branka, Krstić Milošević, Dijana, Jankovic, Teodora, Pljevljakusic, Dejan, Ninković, Slavica, Smigocki, Ann, Vinterhalter, Dragan, "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):667-679,
https://doi.org/10.1007/s11240-015-0737-z . .