TY  - JOUR
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Radulovacki, Miodrag
AU  - Carley, David W
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1027
AB  - We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.
T2  - Sleep and Biological Rhythms
T1  - Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder
IS  - 2
VL  - 11
SP  - 243
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1027
ER  - 

@article{
author = "Šaponjić, Jasna and Petrović, Jelena and Kalauzi, Aleksandar and Ćirić, Jelena and Lazić, Katarina and Radulovacki, Miodrag and Carley, David W",
year = "2013",
abstract = "We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.",
journal = "Sleep and Biological Rhythms",
title = "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder",
number = "2",
volume = "11",
pages = "243-115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1027"
}

Šaponjić, J., Petrović, J., Kalauzi, A., Ćirić, J., Lazić, K., Radulovacki, M.,& Carley, D. W.. (2013). Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms, 11(2), 243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027

Šaponjić J, Petrović J, Kalauzi A, Ćirić J, Lazić K, Radulovacki M, Carley DW. Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms. 2013;11(2):243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

Šaponjić, Jasna, Petrović, Jelena, Kalauzi, Aleksandar, Ćirić, Jelena, Lazić, Katarina, Radulovacki, Miodrag, Carley, David W, "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder" in Sleep and Biological Rhythms, 11, no. 2 (2013):243-115,
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

TY  - JOUR
AU  - Kesić, Srđan
AU  - Kalauzi, Aleksandar
AU  - Radulovacki, Miodrag
AU  - Carley, David W
AU  - Šaponjić, Jasna
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1330
AB  - Sigma and theta frequency electroencephalogram (EEG) oscillations exhibit substantial and well-recognized shifts with transitions across sleep and wake states. We aimed in this study to test the changes in coupling between these characteristic oscillations of non-rapid-eye-movement (NREM)/rapid-eye-movement (REM) sleep within and between cortical and pontine EEGs following monoaminergic lesion, by using the Pearson's product-moment correlation coefficients. Experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording. We lesioned the dorsal raphe nucleus axon terminals in four rats using PCA neurotoxin (p-chloroamphetamine; Sigma-Aldrich, MO) administered as two intraperitoneal (IP) injections (6 mg/kg) 24 h apart. Lesioning of locus coeruleus axon terminals was performed in five rats using DSP-4 neurotoxin (N-2-chloroethyl-N-ethyl-2-bromobenzilamine; Sigma-Aldrich, MO) in a single IP dose of 50 mg/kg. Our previous study [Saponjic et al., Physiol Behav 90:1-10, 2007] demonstrated that these systemically induced monoaminergic lesions failed to produce significant changes in sleep/wake distribution from control conditions. The present study, by using spectral analysis and by examining the Pearson's correlation coefficients and their approximate probability density (APD) distribution profiles in control and lesion condition, demonstrates significant augmentation of the sigma/theta coupling strength, an inversion of cortical sigma/theta coupling direction and emergence of an additional sigma/theta coupling "mode" specific to the post-lesion state only within the cortex. By using the Pearson's correlation coefficients and their APD profiles, instead of classical sleep/wake distribution analysis, as a measure of direction and strength of sigma/theta coupling within and between cortex and pons, we were able to uncover the impact of a tonically decreased level of brain monoamines as altered strength and mode of coupling between sigma and theta oscillations. Specifically, a new mode of sigma/theta coupling emerged following lesion, which was specific to NREM sleep, suggests that loss of monoaminergic signaling interferes with NREM sleep consolidation. Our results also indicate an importance of monoamines in control of the sleep spindle and theta rhythm generators.
T2  - Sleep and Breathing
T1  - Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat
IS  - 1
VL  - 15
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1330
ER  - 

@article{
author = "Kesić, Srđan and Kalauzi, Aleksandar and Radulovacki, Miodrag and Carley, David W and Šaponjić, Jasna",
year = "2011",
abstract = "Sigma and theta frequency electroencephalogram (EEG) oscillations exhibit substantial and well-recognized shifts with transitions across sleep and wake states. We aimed in this study to test the changes in coupling between these characteristic oscillations of non-rapid-eye-movement (NREM)/rapid-eye-movement (REM) sleep within and between cortical and pontine EEGs following monoaminergic lesion, by using the Pearson's product-moment correlation coefficients. Experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording. We lesioned the dorsal raphe nucleus axon terminals in four rats using PCA neurotoxin (p-chloroamphetamine; Sigma-Aldrich, MO) administered as two intraperitoneal (IP) injections (6 mg/kg) 24 h apart. Lesioning of locus coeruleus axon terminals was performed in five rats using DSP-4 neurotoxin (N-2-chloroethyl-N-ethyl-2-bromobenzilamine; Sigma-Aldrich, MO) in a single IP dose of 50 mg/kg. Our previous study [Saponjic et al., Physiol Behav 90:1-10, 2007] demonstrated that these systemically induced monoaminergic lesions failed to produce significant changes in sleep/wake distribution from control conditions. The present study, by using spectral analysis and by examining the Pearson's correlation coefficients and their approximate probability density (APD) distribution profiles in control and lesion condition, demonstrates significant augmentation of the sigma/theta coupling strength, an inversion of cortical sigma/theta coupling direction and emergence of an additional sigma/theta coupling "mode" specific to the post-lesion state only within the cortex. By using the Pearson's correlation coefficients and their APD profiles, instead of classical sleep/wake distribution analysis, as a measure of direction and strength of sigma/theta coupling within and between cortex and pons, we were able to uncover the impact of a tonically decreased level of brain monoamines as altered strength and mode of coupling between sigma and theta oscillations. Specifically, a new mode of sigma/theta coupling emerged following lesion, which was specific to NREM sleep, suggests that loss of monoaminergic signaling interferes with NREM sleep consolidation. Our results also indicate an importance of monoamines in control of the sleep spindle and theta rhythm generators.",
journal = "Sleep and Breathing",
title = "Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat",
number = "1",
volume = "15",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1330"
}

Kesić, S., Kalauzi, A., Radulovacki, M., Carley, D. W.,& Šaponjić, J.. (2011). Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat. in Sleep and Breathing, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1330

Kesić S, Kalauzi A, Radulovacki M, Carley DW, Šaponjić J. Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat. in Sleep and Breathing. 2011;15(1):null-47.
https://hdl.handle.net/21.15107/rcub_ibiss_1330 .

Kesić, Srđan, Kalauzi, Aleksandar, Radulovacki, Miodrag, Carley, David W, Šaponjić, Jasna, "Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat" in Sleep and Breathing, 15, no. 1 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1330 .

TY  - JOUR
AU  - Bojić, Tijana
AU  - Šaponjić, Jasna
AU  - Radulovacki, Miodrag
AU  - Carley, David W
AU  - Kalauzi, Aleksandar
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1529
AB  - We applied a novel approach to respiratory waveform analysis-Monotone Signal Segments Analysis (MSSA) on 6-h recordings of respiratory signals in rats. To validate MSSA as a respiratory signal analysis tool we tested it by detecting: breaths and breath-to-breath intervals; respiratory timing and volume modes; and changes in respiratory pattern caused by lesions of monoaminergic systems in rats. MSSA differentiated three respiratory timing (tachypneic, eupneic, bradypneic-apneic), and three volume (artifacts, normovolemic, hypervolemic-sighs) modes. Lesion-induced respiratory pattern modulation was visible as shifts in the distributions of monotone signal segment amplitudes, and of breath-to-breath intervals. Specifically, noradrenergic lesion induced an increase in mean volume (p <= 0.03), with no change of the mean breath-to-breath interval duration (p >= 0.06). MSSA of timing modes detected noradrenergic lesion-induced interdependent changes in the balance of eupneic (decrease; p <= 0.02), and tachypneic (an increase; p <= 0.02) breath intervals with respect to control. In terms of breath durations within each timing mode, there was a tendency toward prolongation of the eupneic (p <= 0.08) and bradypneic-apneic (p <= 0.06) intervals. These results demonstrate that MSSA is sensitive to subtle shifts in respiratory rhythmogenesis not detectable by simple respiratory pattern descriptive statistics. MSSA represents a potentially valuable new tool for investigations of respiratory pattern control. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Respiratory Physiology & Neurobiology
T1  - Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat
IS  - 3
VL  - 161
EP  - 280
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1529
ER  - 

@article{
author = "Bojić, Tijana and Šaponjić, Jasna and Radulovacki, Miodrag and Carley, David W and Kalauzi, Aleksandar",
year = "2008",
abstract = "We applied a novel approach to respiratory waveform analysis-Monotone Signal Segments Analysis (MSSA) on 6-h recordings of respiratory signals in rats. To validate MSSA as a respiratory signal analysis tool we tested it by detecting: breaths and breath-to-breath intervals; respiratory timing and volume modes; and changes in respiratory pattern caused by lesions of monoaminergic systems in rats. MSSA differentiated three respiratory timing (tachypneic, eupneic, bradypneic-apneic), and three volume (artifacts, normovolemic, hypervolemic-sighs) modes. Lesion-induced respiratory pattern modulation was visible as shifts in the distributions of monotone signal segment amplitudes, and of breath-to-breath intervals. Specifically, noradrenergic lesion induced an increase in mean volume (p <= 0.03), with no change of the mean breath-to-breath interval duration (p >= 0.06). MSSA of timing modes detected noradrenergic lesion-induced interdependent changes in the balance of eupneic (decrease; p <= 0.02), and tachypneic (an increase; p <= 0.02) breath intervals with respect to control. In terms of breath durations within each timing mode, there was a tendency toward prolongation of the eupneic (p <= 0.08) and bradypneic-apneic (p <= 0.06) intervals. These results demonstrate that MSSA is sensitive to subtle shifts in respiratory rhythmogenesis not detectable by simple respiratory pattern descriptive statistics. MSSA represents a potentially valuable new tool for investigations of respiratory pattern control. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Respiratory Physiology & Neurobiology",
title = "Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat",
number = "3",
volume = "161",
pages = "280",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1529"
}

Bojić, T., Šaponjić, J., Radulovacki, M., Carley, D. W.,& Kalauzi, A.. (2008). Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat. in Respiratory Physiology & Neurobiology, 161(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1529

Bojić T, Šaponjić J, Radulovacki M, Carley DW, Kalauzi A. Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat. in Respiratory Physiology & Neurobiology. 2008;161(3):null-280.
https://hdl.handle.net/21.15107/rcub_ibiss_1529 .

Bojić, Tijana, Šaponjić, Jasna, Radulovacki, Miodrag, Carley, David W, Kalauzi, Aleksandar, "Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat" in Respiratory Physiology & Neurobiology, 161, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1529 .