TY  - JOUR
AU  - Robbemont, Jody
AU  - van Veldhuijzen, Sam
AU  - Allain, Steven J.R.
AU  - Ambu, Johanna
AU  - Boyle, Ryan
AU  - Canestrelli, Daniele
AU  - Cathasaigh, Éinne Ó
AU  - Cathrine, Chris
AU  - Chiocchio, Andrea
AU  - Cogalniceanu, Dan
AU  - Cvijanović, Milena
AU  - Dufresnes, Christophe
AU  - Ennis, Collie
AU  - Gandola, Rob
AU  - Jablonski, Daniel
AU  - Julian, Angela
AU  - Kranželić, Daria
AU  - Lukanov, Simeon
AU  - Martínez-Solano, Iñigo
AU  - Montgomery, Ryan
AU  - Naumov, Borislav
AU  - O’Neill, Matthew
AU  - North, Alexandra
AU  - Pabijan, Maciej
AU  - Pushendorf, Robert
AU  - Salvi, Daniele
AU  - Schmidt, Bruno
AU  - Sotiropoulos, Konstantinos
AU  - Stanescu, Florina
AU  - Stanković, David
AU  - Stapleton, Sarah
AU  - Šunje, Emina
AU  - Szabolcs, Márton
AU  - Vacheva, Emiliya
AU  - Willis, David
AU  - Zimić, Adnan
AU  - France, James
AU  - Meilink, Willem R.M.
AU  - Stark, Tariq
AU  - Struijk, Richard P.J.H.
AU  - Theodoropoulos, Anagnostis
AU  - de Visser, Manon C.
AU  - Wielstra, Ben
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6052
AB  - Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding.
PB  - Brill Academic Publishers
T2  - Amphibia-Reptilia
T1  - An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations
IS  - 3
VL  - 44
DO  - 10.1163/15685381-bja10144
SP  - 347
EP  - 361
ER  - 

@article{
author = "Robbemont, Jody and van Veldhuijzen, Sam and Allain, Steven J.R. and Ambu, Johanna and Boyle, Ryan and Canestrelli, Daniele and Cathasaigh, Éinne Ó and Cathrine, Chris and Chiocchio, Andrea and Cogalniceanu, Dan and Cvijanović, Milena and Dufresnes, Christophe and Ennis, Collie and Gandola, Rob and Jablonski, Daniel and Julian, Angela and Kranželić, Daria and Lukanov, Simeon and Martínez-Solano, Iñigo and Montgomery, Ryan and Naumov, Borislav and O’Neill, Matthew and North, Alexandra and Pabijan, Maciej and Pushendorf, Robert and Salvi, Daniele and Schmidt, Bruno and Sotiropoulos, Konstantinos and Stanescu, Florina and Stanković, David and Stapleton, Sarah and Šunje, Emina and Szabolcs, Márton and Vacheva, Emiliya and Willis, David and Zimić, Adnan and France, James and Meilink, Willem R.M. and Stark, Tariq and Struijk, Richard P.J.H. and Theodoropoulos, Anagnostis and de Visser, Manon C. and Wielstra, Ben",
year = "2023",
abstract = "Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding.",
publisher = "Brill Academic Publishers",
journal = "Amphibia-Reptilia",
title = "An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations",
number = "3",
volume = "44",
doi = "10.1163/15685381-bja10144",
pages = "347-361"
}

Robbemont, J., van Veldhuijzen, S., Allain, S. J.R., Ambu, J., Boyle, R., Canestrelli, D., Cathasaigh, É. Ó., Cathrine, C., Chiocchio, A., Cogalniceanu, D., Cvijanović, M., Dufresnes, C., Ennis, C., Gandola, R., Jablonski, D., Julian, A., Kranželić, D., Lukanov, S., Martínez-Solano, I., Montgomery, R., Naumov, B., O’Neill, M., North, A., Pabijan, M., Pushendorf, R., Salvi, D., Schmidt, B., Sotiropoulos, K., Stanescu, F., Stanković, D., Stapleton, S., Šunje, E., Szabolcs, M., Vacheva, E., Willis, D., Zimić, A., France, J., Meilink, W. R.M., Stark, T., Struijk, R. P.J.H., Theodoropoulos, A., de Visser, M. C.,& Wielstra, B.. (2023). An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations. in Amphibia-Reptilia
Brill Academic Publishers., 44(3), 347-361.
https://doi.org/10.1163/15685381-bja10144

Robbemont J, van Veldhuijzen S, Allain SJ, Ambu J, Boyle R, Canestrelli D, Cathasaigh ÉÓ, Cathrine C, Chiocchio A, Cogalniceanu D, Cvijanović M, Dufresnes C, Ennis C, Gandola R, Jablonski D, Julian A, Kranželić D, Lukanov S, Martínez-Solano I, Montgomery R, Naumov B, O’Neill M, North A, Pabijan M, Pushendorf R, Salvi D, Schmidt B, Sotiropoulos K, Stanescu F, Stanković D, Stapleton S, Šunje E, Szabolcs M, Vacheva E, Willis D, Zimić A, France J, Meilink WR, Stark T, Struijk RP, Theodoropoulos A, de Visser MC, Wielstra B. An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations. in Amphibia-Reptilia. 2023;44(3):347-361.
doi:10.1163/15685381-bja10144 .

Robbemont, Jody, van Veldhuijzen, Sam, Allain, Steven J.R., Ambu, Johanna, Boyle, Ryan, Canestrelli, Daniele, Cathasaigh, Éinne Ó, Cathrine, Chris, Chiocchio, Andrea, Cogalniceanu, Dan, Cvijanović, Milena, Dufresnes, Christophe, Ennis, Collie, Gandola, Rob, Jablonski, Daniel, Julian, Angela, Kranželić, Daria, Lukanov, Simeon, Martínez-Solano, Iñigo, Montgomery, Ryan, Naumov, Borislav, O’Neill, Matthew, North, Alexandra, Pabijan, Maciej, Pushendorf, Robert, Salvi, Daniele, Schmidt, Bruno, Sotiropoulos, Konstantinos, Stanescu, Florina, Stanković, David, Stapleton, Sarah, Šunje, Emina, Szabolcs, Márton, Vacheva, Emiliya, Willis, David, Zimić, Adnan, France, James, Meilink, Willem R.M., Stark, Tariq, Struijk, Richard P.J.H., Theodoropoulos, Anagnostis, de Visser, Manon C., Wielstra, Ben, "An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations" in Amphibia-Reptilia, 44, no. 3 (2023):347-361,
https://doi.org/10.1163/15685381-bja10144 . .

TY  - JOUR
AU  - Dufresnes, Christophe
AU  - Strachinis, Ilias
AU  - Suriadna, Nataliia
AU  - Mykytynets, Galyna
AU  - Cogălniceanu, Dan
AU  - Székely, Paul
AU  - Vukov, Tanja
AU  - Arntzen, Jan W.
AU  - Wielstra, Ben
AU  - Lymberakis, Petros
AU  - Geffen, Eli
AU  - Gafny, Sarig
AU  - Kumlutaş, Yusuf
AU  - Ilgaz, Çetin
AU  - Candan, Kamil
AU  - Mizsei, Edvárd
AU  - Szabolcs, Márton
AU  - Kolenda, Krzysztof
AU  - Smirnov, Nazar
AU  - Géniez, Philippe
AU  - Lukanov, Simeon
AU  - Crochet, Pierre-André
AU  - Dubey, Sylvain
AU  - Perrin, Nicolas
AU  - Litvinchuk, Spartak N.
AU  - Denoël, Mathieu
PY  - 2019
UR  - http://doi.wiley.com/10.1111/mec.15133
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3412
AB  - Cryptic phylogeographic diversifications provide unique models to examine the role of phylogenetic divergence on the evolution of reproductive isolation, without extrinsic factors such as ecological and behavioural differentiation. Yet, to date very few comparative studies have been attempted within such radiations. Here, we characterize a new speciation continuum in a group of widespread Eurasian amphibians, the Pelobates spadefoot toads, by conducting multilocus (restriction site associated DNA sequencing and mitochondrial DNA) phylogenetic, phylogeographic and hybrid zone analyses. Within the P. syriacus complex, we discovered species-level cryptic divergences (>5 million years ago [My]) between populations distributed in the Near-East (hereafter P. syriacus sensu stricto [s.s.]) and southeastern Europe (hereafter P. balcanicus), each featuring deep intraspecific lineages. Altogether, we could scale hybridizability to divergence time along six different stages, spanning from sympatry without gene flow (P. fuscus and P. balcanicus, >10 My), parapatry with highly restricted hybridization (P. balcanicus and P. syriacus s.s., >5 My), narrow hybrid zones (~15 km) consistent with partial reproductive isolation (P. fuscus and P. vespertinus, ~3 My), to extensive admixture between Pleistocene and refugial lineages (≤2 My). This full spectrum empirically supports a gradual build up of reproductive barriers through time, reversible up until a threshold that we estimate at ~3 My. Hence, cryptic phylogeographic lineages may fade away or become reproductively isolated species simply depending on the time they persist in allopatry, and without definite ecomorphological divergence.
T2  - Molecular Ecology
T1  - Phylogeography of a cryptic speciation continuum in Eurasian spadefoot toads (Pelobates).
DO  - 10.1111/mec.15133
ER  - 

@article{
author = "Dufresnes, Christophe and Strachinis, Ilias and Suriadna, Nataliia and Mykytynets, Galyna and Cogălniceanu, Dan and Székely, Paul and Vukov, Tanja and Arntzen, Jan W. and Wielstra, Ben and Lymberakis, Petros and Geffen, Eli and Gafny, Sarig and Kumlutaş, Yusuf and Ilgaz, Çetin and Candan, Kamil and Mizsei, Edvárd and Szabolcs, Márton and Kolenda, Krzysztof and Smirnov, Nazar and Géniez, Philippe and Lukanov, Simeon and Crochet, Pierre-André and Dubey, Sylvain and Perrin, Nicolas and Litvinchuk, Spartak N. and Denoël, Mathieu",
year = "2019",
abstract = "Cryptic phylogeographic diversifications provide unique models to examine the role of phylogenetic divergence on the evolution of reproductive isolation, without extrinsic factors such as ecological and behavioural differentiation. Yet, to date very few comparative studies have been attempted within such radiations. Here, we characterize a new speciation continuum in a group of widespread Eurasian amphibians, the Pelobates spadefoot toads, by conducting multilocus (restriction site associated DNA sequencing and mitochondrial DNA) phylogenetic, phylogeographic and hybrid zone analyses. Within the P. syriacus complex, we discovered species-level cryptic divergences (>5 million years ago [My]) between populations distributed in the Near-East (hereafter P. syriacus sensu stricto [s.s.]) and southeastern Europe (hereafter P. balcanicus), each featuring deep intraspecific lineages. Altogether, we could scale hybridizability to divergence time along six different stages, spanning from sympatry without gene flow (P. fuscus and P. balcanicus, >10 My), parapatry with highly restricted hybridization (P. balcanicus and P. syriacus s.s., >5 My), narrow hybrid zones (~15 km) consistent with partial reproductive isolation (P. fuscus and P. vespertinus, ~3 My), to extensive admixture between Pleistocene and refugial lineages (≤2 My). This full spectrum empirically supports a gradual build up of reproductive barriers through time, reversible up until a threshold that we estimate at ~3 My. Hence, cryptic phylogeographic lineages may fade away or become reproductively isolated species simply depending on the time they persist in allopatry, and without definite ecomorphological divergence.",
journal = "Molecular Ecology",
title = "Phylogeography of a cryptic speciation continuum in Eurasian spadefoot toads (Pelobates).",
doi = "10.1111/mec.15133"
}

Dufresnes, C., Strachinis, I., Suriadna, N., Mykytynets, G., Cogălniceanu, D., Székely, P., Vukov, T., Arntzen, J. W., Wielstra, B., Lymberakis, P., Geffen, E., Gafny, S., Kumlutaş, Y., Ilgaz, Ç., Candan, K., Mizsei, E., Szabolcs, M., Kolenda, K., Smirnov, N., Géniez, P., Lukanov, S., Crochet, P., Dubey, S., Perrin, N., Litvinchuk, S. N.,& Denoël, M.. (2019). Phylogeography of a cryptic speciation continuum in Eurasian spadefoot toads (Pelobates).. in Molecular Ecology.
https://doi.org/10.1111/mec.15133

Dufresnes C, Strachinis I, Suriadna N, Mykytynets G, Cogălniceanu D, Székely P, Vukov T, Arntzen JW, Wielstra B, Lymberakis P, Geffen E, Gafny S, Kumlutaş Y, Ilgaz Ç, Candan K, Mizsei E, Szabolcs M, Kolenda K, Smirnov N, Géniez P, Lukanov S, Crochet P, Dubey S, Perrin N, Litvinchuk SN, Denoël M. Phylogeography of a cryptic speciation continuum in Eurasian spadefoot toads (Pelobates).. in Molecular Ecology. 2019;.
doi:10.1111/mec.15133 .

Dufresnes, Christophe, Strachinis, Ilias, Suriadna, Nataliia, Mykytynets, Galyna, Cogălniceanu, Dan, Székely, Paul, Vukov, Tanja, Arntzen, Jan W., Wielstra, Ben, Lymberakis, Petros, Geffen, Eli, Gafny, Sarig, Kumlutaş, Yusuf, Ilgaz, Çetin, Candan, Kamil, Mizsei, Edvárd, Szabolcs, Márton, Kolenda, Krzysztof, Smirnov, Nazar, Géniez, Philippe, Lukanov, Simeon, Crochet, Pierre-André, Dubey, Sylvain, Perrin, Nicolas, Litvinchuk, Spartak N., Denoël, Mathieu, "Phylogeography of a cryptic speciation continuum in Eurasian spadefoot toads (Pelobates)." in Molecular Ecology (2019),
https://doi.org/10.1111/mec.15133 . .