TY  - BOOK
AU  - Nešković, Mirjana
AU  - Ninković, Slavica
AU  - Miljuš Đukić, Jovanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5028
AB  - U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive
SP  - 1
EP  - 406
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5028
ER  - 

@book{
author = "Nešković, Mirjana and Ninković, Slavica and Miljuš Đukić, Jovanka",
year = "2022",
abstract = "U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive",
pages = "1-406",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5028"
}

Nešković, M., Ninković, S.,& Miljuš Đukić, J.. (2022). Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028

Nešković M, Ninković S, Miljuš Đukić J. Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 2022;:1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

Nešković, Mirjana, Ninković, Slavica, Miljuš Đukić, Jovanka, "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive" (2022):1-406,
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

TY  - JOUR
AU  - Nikolić, Radomirka R
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Nešković, Mirjana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1337
AB  - Shoots of Lotus corniculatus L., previously transformed with Agrobacterium tumefaciens LBA4404/pTOK233, were grown in gibberellic acid (GA(3))-containing media in an attempt to improve their growth and multiplication. Nodal stem segments of four poorly multiplying clones, a clone with very good multiplication, and two non-transformed clones were incubated in media containing GA(3) for 3 weeks, and then returned to a hormone-free medium for a further 3-5 subculture periods. Gibberellic acid increased the number of axillary buds from one in controls to 2-13 shoots during the first 3 weeks of subculture in GA(3)-containing media. The multiplied buds, which apparently derived from additional axillaries, continued to multiply in hormone-free medium, producing bunches of more than 50 shoots after 9 weeks. In addition, many nodal segments also produced callus tissues at the basal end, or along the internode below the node, in which abundant shoot meristems formed de novo. Histological examination confirmed their origin via organogenesis. About 25% of regenerated shoots rooted spontaneously. The application of auxins significantly improved rooting in more than 75% of all clones. Well-developed plantlets were produced upon the transfer of rooted shoots to pots. No differences in responses to hormones were observed between LBA4404/pTOK233-transformed clones and non-transformed clones, indicating that the reaction to GA(3) was not the consequence of transformation events. The results point to a possible involvement of GA(3) in lateral branching. They might also be recommended as a procedure suitable for increasing the production of transformed plants in L. corniculatus.
T2  - Plant Growth Regulation
T1  - Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.
IS  - 2
VL  - 62
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1337
ER  - 

@article{
author = "Nikolić, Radomirka R and Banjac, Nevena and Ninković, Slavica and Vinterhalter, Branka and Zdravković-Korać, Snežana and Nešković, Mirjana",
year = "2010",
abstract = "Shoots of Lotus corniculatus L., previously transformed with Agrobacterium tumefaciens LBA4404/pTOK233, were grown in gibberellic acid (GA(3))-containing media in an attempt to improve their growth and multiplication. Nodal stem segments of four poorly multiplying clones, a clone with very good multiplication, and two non-transformed clones were incubated in media containing GA(3) for 3 weeks, and then returned to a hormone-free medium for a further 3-5 subculture periods. Gibberellic acid increased the number of axillary buds from one in controls to 2-13 shoots during the first 3 weeks of subculture in GA(3)-containing media. The multiplied buds, which apparently derived from additional axillaries, continued to multiply in hormone-free medium, producing bunches of more than 50 shoots after 9 weeks. In addition, many nodal segments also produced callus tissues at the basal end, or along the internode below the node, in which abundant shoot meristems formed de novo. Histological examination confirmed their origin via organogenesis. About 25% of regenerated shoots rooted spontaneously. The application of auxins significantly improved rooting in more than 75% of all clones. Well-developed plantlets were produced upon the transfer of rooted shoots to pots. No differences in responses to hormones were observed between LBA4404/pTOK233-transformed clones and non-transformed clones, indicating that the reaction to GA(3) was not the consequence of transformation events. The results point to a possible involvement of GA(3) in lateral branching. They might also be recommended as a procedure suitable for increasing the production of transformed plants in L. corniculatus.",
journal = "Plant Growth Regulation",
title = "Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.",
number = "2",
volume = "62",
pages = "188",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1337"
}

Nikolić, R. R., Banjac, N., Ninković, S., Vinterhalter, B., Zdravković-Korać, S.,& Nešković, M.. (2010). Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.. in Plant Growth Regulation, 62(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1337

Nikolić RR, Banjac N, Ninković S, Vinterhalter B, Zdravković-Korać S, Nešković M. Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.. in Plant Growth Regulation. 2010;62(2):null-188.
https://hdl.handle.net/21.15107/rcub_ibiss_1337 .

Nikolić, Radomirka R, Banjac, Nevena, Ninković, Slavica, Vinterhalter, Branka, Zdravković-Korać, Snežana, Nešković, Mirjana, "Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L." in Plant Growth Regulation, 62, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1337 .

TY  - JOUR
AU  - Dragicević, Ivana C
AU  - Konjević, Radomir M
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Nešković, Mirjana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1540
AB  - Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.
T2  - Plant Growth Regulation
T1  - The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro
IS  - 3
VL  - 54
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1540
ER  - 

@article{
author = "Dragicević, Ivana C and Konjević, Radomir M and Vinterhalter, Branka and Vinterhalter, Dragan V and Nešković, Mirjana",
year = "2008",
abstract = "Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.",
journal = "Plant Growth Regulation",
title = "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro",
number = "3",
volume = "54",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1540"
}

Dragicević, I. C., Konjević, R. M., Vinterhalter, B., Vinterhalter, D. V.,& Nešković, M.. (2008). The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation, 54(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1540

Dragicević IC, Konjević RM, Vinterhalter B, Vinterhalter DV, Nešković M. The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation. 2008;54(3):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

Dragicević, Ivana C, Konjević, Radomir M, Vinterhalter, Branka, Vinterhalter, Dragan V, Nešković, Mirjana, "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro" in Plant Growth Regulation, 54, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

TY  - JOUR
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Nešković, Mirjana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/180
AB  - Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation. The explants were co-cultivated with the Agrobacterium tumefaciens LBA4404/pTOK233 superbinary vector carrying the uidA-intron gene and the genes hpt and nptII. They were cultured for 14 days on a regeneration medium, then subjected to a stepwise hygromycin B selection procedure consisting of gradually increasing antibiotic concentrations (5-15 mg L-1) over 21 weeks. Transformed shoots were obtained within 5 months after co-cultivation. Out of 124 initially co-cultivated explants, 52 (42%) plants survived hygromycin B selection. The presence of transgenes in regenerated plants was verified by β-glucuronidase histochemical assays and PCR analysis for the presence of uidA gene sequences. Hygromycin B-resistant and PCR-positive T0 plants were cultured in the greenhouse to produce flowers and seeds. The obtained data demonstrate that the reported transformation protocol could be useful for introducing agriculturally important genes into the new L. corniculatus cultivar Bokor.
AB  - Kotiledoni, izolovani sa 6 dana starih klijanaca Lotus corniculatus, sorta Bokor, su se pokazali kao pogodni eksplantati za regeneraciju biljaka bez intermedijarne kalusne faze. Kotiledoni su presecani transverzalno na dve polovine i ko-kultivisani sa Agrobacterium tumefaciens superbinarnim vektorom LBA4404/pTOK233, koji nosi uidA-intron, hpt i nptII gene. Posle ko-kultivacije eksplantati su prvih 14 dana gajeni na regenerativnom medijumu bez selektivnog agensa, a zatim su 21 nedelju bili podvrgnuti selektivnoj proceduri tokom koje je koncentracija antibiotika postepeno povećavana (5-15 mg L-1). Transformisani izdanci dobijeni su 5 meseci nakon ko-kultivacije. Od 124 inicijalno ko-kultivisana eksplantata, dobijene su 52 (42%) biljke koje su preživele selekciju na higromicinu B. Prisustvo uidA transgena u regenerisanim biljkama je potvrđeno pomoću β-glukuronidazne histohemijske reakcije i PCR analizom. T0 biljke, rezistentne na higromicin B i PCR pozitivne, gajene su u stakleniku u cilju produkcije i rastenja cvetova i semena. Dobijenim rezultatima je jasno ukazano da bi saopšteni protokol za transformaciju mogao nesumnjivo biti upotrebljen za introdukciju agronomski značajnih gena u Lotus corniculatus, sorta Bokor.
T2  - Archives of Biological Sciences
T1  - Efikasna genetička transformacija Lotus corniculatus L. pomoću direktne regeneracije biljaka, postepene selekcije sa higromicinom B i Agrobacterium tumefaciens super-binarnog vektora
T1  - Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector
IS  - 4
VL  - 59
SP  - 311
EP  - 317
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_180
ER  - 

@article{
author = "Nikolić, Radomirka and Banjac, Nevena and Ninković, Slavica and Nešković, Mirjana",
year = "2007, 2007",
abstract = "Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation. The explants were co-cultivated with the Agrobacterium tumefaciens LBA4404/pTOK233 superbinary vector carrying the uidA-intron gene and the genes hpt and nptII. They were cultured for 14 days on a regeneration medium, then subjected to a stepwise hygromycin B selection procedure consisting of gradually increasing antibiotic concentrations (5-15 mg L-1) over 21 weeks. Transformed shoots were obtained within 5 months after co-cultivation. Out of 124 initially co-cultivated explants, 52 (42%) plants survived hygromycin B selection. The presence of transgenes in regenerated plants was verified by β-glucuronidase histochemical assays and PCR analysis for the presence of uidA gene sequences. Hygromycin B-resistant and PCR-positive T0 plants were cultured in the greenhouse to produce flowers and seeds. The obtained data demonstrate that the reported transformation protocol could be useful for introducing agriculturally important genes into the new L. corniculatus cultivar Bokor., Kotiledoni, izolovani sa 6 dana starih klijanaca Lotus corniculatus, sorta Bokor, su se pokazali kao pogodni eksplantati za regeneraciju biljaka bez intermedijarne kalusne faze. Kotiledoni su presecani transverzalno na dve polovine i ko-kultivisani sa Agrobacterium tumefaciens superbinarnim vektorom LBA4404/pTOK233, koji nosi uidA-intron, hpt i nptII gene. Posle ko-kultivacije eksplantati su prvih 14 dana gajeni na regenerativnom medijumu bez selektivnog agensa, a zatim su 21 nedelju bili podvrgnuti selektivnoj proceduri tokom koje je koncentracija antibiotika postepeno povećavana (5-15 mg L-1). Transformisani izdanci dobijeni su 5 meseci nakon ko-kultivacije. Od 124 inicijalno ko-kultivisana eksplantata, dobijene su 52 (42%) biljke koje su preživele selekciju na higromicinu B. Prisustvo uidA transgena u regenerisanim biljkama je potvrđeno pomoću β-glukuronidazne histohemijske reakcije i PCR analizom. T0 biljke, rezistentne na higromicin B i PCR pozitivne, gajene su u stakleniku u cilju produkcije i rastenja cvetova i semena. Dobijenim rezultatima je jasno ukazano da bi saopšteni protokol za transformaciju mogao nesumnjivo biti upotrebljen za introdukciju agronomski značajnih gena u Lotus corniculatus, sorta Bokor.",
journal = "Archives of Biological Sciences",
title = "Efikasna genetička transformacija Lotus corniculatus L. pomoću direktne regeneracije biljaka, postepene selekcije sa higromicinom B i Agrobacterium tumefaciens super-binarnog vektora, Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector",
number = "4",
volume = "59",
pages = "311-317",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_180"
}

Nikolić, R., Banjac, N., Ninković, S.,& Nešković, M.. (2007). Efikasna genetička transformacija Lotus corniculatus L. pomoću direktne regeneracije biljaka, postepene selekcije sa higromicinom B i Agrobacterium tumefaciens super-binarnog vektora. in Archives of Biological Sciences, 59(4), 311-317.
https://hdl.handle.net/21.15107/rcub_ibiss_180

Nikolić R, Banjac N, Ninković S, Nešković M. Efikasna genetička transformacija Lotus corniculatus L. pomoću direktne regeneracije biljaka, postepene selekcije sa higromicinom B i Agrobacterium tumefaciens super-binarnog vektora. in Archives of Biological Sciences. 2007;59(4):311-317.
https://hdl.handle.net/21.15107/rcub_ibiss_180 .

Nikolić, Radomirka, Banjac, Nevena, Ninković, Slavica, Nešković, Mirjana, "Efikasna genetička transformacija Lotus corniculatus L. pomoću direktne regeneracije biljaka, postepene selekcije sa higromicinom B i Agrobacterium tumefaciens super-binarnog vektora" in Archives of Biological Sciences, 59, no. 4 (2007):311-317,
https://hdl.handle.net/21.15107/rcub_ibiss_180 .

TY  - JOUR
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Živković, Suzana
AU  - Grubišić, D.
AU  - Nešković, Mirjana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/154
AB  - We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .
AB  - Ispitivan je efekat različitih citokinina i derivata uree na klijanje starih semena Lotus corniculatus L. Upotrebljene su sledeće supstance: N6-izoprenoidni citokinini (izopentenil adenin i zeatin), adenin sulfat, N6-aromatični citokinini (kinetin, benziladenin), i njihovi N9-ribozidi, N-benzil-9- (2-tetrahidropiranil)adenin i derivati uree (difenilurea, tidiazuron i hloro-piridil fenilurea). Svi citokinini su, izuzev adenin sulfata, povećavali procenat klijanja semena do dva puta u zavisnosti od vrste citokinina i koncentracije. Zaključeno je da citokinini mogu biti jedan od nedostajućih faktora u starim semenima L. corniculatus, koji doprinose ostvarenju njihovog punog potencijala za klijanje. Ako je potrebno, citokinini mogu biti iskorišćeni za poboljšanje klijanja sveže ubranih i starih semena. .
T2  - Archives of Biological Sciences
T1  - Citokinini i derivati uree stimulišu klijanje semena Lotus corniculatus L.
T1  - Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.
IS  - 2
VL  - 59
SP  - 125
EP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_154
ER  - 

@article{
author = "Nikolić, Radomirka and Banjac, Nevena and Živković, Suzana and Grubišić, D. and Nešković, Mirjana",
year = "2007, 2007",
abstract = "We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. ., Ispitivan je efekat različitih citokinina i derivata uree na klijanje starih semena Lotus corniculatus L. Upotrebljene su sledeće supstance: N6-izoprenoidni citokinini (izopentenil adenin i zeatin), adenin sulfat, N6-aromatični citokinini (kinetin, benziladenin), i njihovi N9-ribozidi, N-benzil-9- (2-tetrahidropiranil)adenin i derivati uree (difenilurea, tidiazuron i hloro-piridil fenilurea). Svi citokinini su, izuzev adenin sulfata, povećavali procenat klijanja semena do dva puta u zavisnosti od vrste citokinina i koncentracije. Zaključeno je da citokinini mogu biti jedan od nedostajućih faktora u starim semenima L. corniculatus, koji doprinose ostvarenju njihovog punog potencijala za klijanje. Ako je potrebno, citokinini mogu biti iskorišćeni za poboljšanje klijanja sveže ubranih i starih semena. .",
journal = "Archives of Biological Sciences",
title = "Citokinini i derivati uree stimulišu klijanje semena Lotus corniculatus L., Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.",
number = "2",
volume = "59",
pages = "125-128",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_154"
}

Nikolić, R., Banjac, N., Živković, S., Grubišić, D.,& Nešković, M.. (2007). Citokinini i derivati uree stimulišu klijanje semena Lotus corniculatus L.. in Archives of Biological Sciences, 59(2), 125-128.
https://hdl.handle.net/21.15107/rcub_ibiss_154

Nikolić R, Banjac N, Živković S, Grubišić D, Nešković M. Citokinini i derivati uree stimulišu klijanje semena Lotus corniculatus L.. in Archives of Biological Sciences. 2007;59(2):125-128.
https://hdl.handle.net/21.15107/rcub_ibiss_154 .

Nikolić, Radomirka, Banjac, Nevena, Živković, Suzana, Grubišić, D., Nešković, Mirjana, "Citokinini i derivati uree stimulišu klijanje semena Lotus corniculatus L." in Archives of Biological Sciences, 59, no. 2 (2007):125-128,
https://hdl.handle.net/21.15107/rcub_ibiss_154 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1747
AB  - The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.
T2  - Biologia Plantarum
T1  - Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.
IS  - 2
VL  - 48
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1747
ER  - 

@article{
author = "Banjac, Nevena and Nikolić, Radomirka R and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Nešković, Mirjana",
year = "2004",
abstract = "The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.",
journal = "Biologia Plantarum",
title = "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.",
number = "2",
volume = "48",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1747"
}

Banjac, N., Nikolić, R. R., Ninković, S., Miljuš-Đukić, J. D.,& Nešković, M.. (2004). Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1747

Banjac N, Nikolić RR, Ninković S, Miljuš-Đukić JD, Nešković M. Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum. 2004;48(2):null-184.
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .

Banjac, Nevena, Nikolić, Radomirka R, Ninković, Slavica, Miljuš-Đukić, Jovanka D., Nešković, Mirjana, "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L." in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka D.
AU  - Ninković, Slavica
AU  - Radović, Svetlana R
AU  - Maksimović, Vesna R
AU  - Brkljačić, Jelena M.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1748
AB  - Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.
T2  - Biologia Plantarum
T1  - Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat
IS  - 2
VL  - 48
EP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1748
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka D. and Ninković, Slavica and Radović, Svetlana R and Maksimović, Vesna R and Brkljačić, Jelena M. and Nešković, Mirjana",
year = "2004",
abstract = "Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.",
journal = "Biologia Plantarum",
title = "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat",
number = "2",
volume = "48",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1748"
}

Miljuš-Đukić, J. D., Ninković, S., Radović, S. R., Maksimović, V. R., Brkljačić, J. M.,& Nešković, M.. (2004). Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1748

Miljuš-Đukić JD, Ninković S, Radović SR, Maksimović VR, Brkljačić JM, Nešković M. Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum. 2004;48(2):null-296.
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .

Miljuš-Đukić, Jovanka D., Ninković, Slavica, Radović, Svetlana R, Maksimović, Vesna R, Brkljačić, Jelena M., Nešković, Mirjana, "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat" in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Nešković, Mirjana
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1822
AB  - Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 - 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 to 74.1 mu mol m(-2) s(-1), leaf area increased 5-fold. Sucrose also significantly increased leaf area and the maxima were at concentration 147 mM at high irradiance and 233.6 mM at low irradiance. Sucrose was superior to Fructose, glucose and combination of both in increasing leaf area. Decreasing concentration of KNO3 and NH4NO3 caused a 3-fold decline of leaf area.
T2  - Biologia Plantarum
T1  - Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.
IS  - 2
VL  - 44
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1822
ER  - 

@article{
author = "Vinterhalter, Branka and Vinterhalter, Dragan V and Nešković, Mirjana",
year = "2001",
abstract = "Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 - 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 to 74.1 mu mol m(-2) s(-1), leaf area increased 5-fold. Sucrose also significantly increased leaf area and the maxima were at concentration 147 mM at high irradiance and 233.6 mM at low irradiance. Sucrose was superior to Fructose, glucose and combination of both in increasing leaf area. Decreasing concentration of KNO3 and NH4NO3 caused a 3-fold decline of leaf area.",
journal = "Biologia Plantarum",
title = "Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.",
number = "2",
volume = "44",
pages = "188",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1822"
}

Vinterhalter, B., Vinterhalter, D. V.,& Nešković, M.. (2001). Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.. in Biologia Plantarum, 44(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1822

Vinterhalter B, Vinterhalter DV, Nešković M. Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.. in Biologia Plantarum. 2001;44(2):null-188.
https://hdl.handle.net/21.15107/rcub_ibiss_1822 .

Vinterhalter, Branka, Vinterhalter, Dragan V, Nešković, Mirjana, "Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L." in Biologia Plantarum, 44, no. 2 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1822 .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Nešković, Mirjana
PY  - 1999
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6168
AB  - Somatic embryogenesis was induced in embryogenic calli of Spinacia oleracea L., on a Murashige and Skoog (1962) medium, containing 4.6 μM kinetin as the sole growth regulator. Abscisic acid, gibberellic acid, and indole-3-acetic acid were supplemented to kinetin-containing medium and their effects on the initiation of somatic embryos was studied. Abscisic acid at a particular concentration (4 μM) dramatically increased the number of embryos per g fresh weight of callus, while both gibberellic acid and indole-3-acetic acid suppressed the embryo initiation. It is suggested that the promoting effect of abscisic acid on the embryo initiation may be explained as a stress response of the tissue. The relative number of globular embryos vs. the embryos in heart/torpedo and cotyledonary stages was increased at 4 μM abscisic acid and at all gibberellic acid concentrations (0.3–10 μM). In contrast, the ratio of globular to polar embryos was lower than in controls at 1 μM abscisic acid and indole-3-acetic acid (1 and 10 μM). The effects of growth regulators on the ratio of globular to polar embryos indicate that they interfere with the normal distribution of cell division and cell expansion during early embryogenesis.
PB  - Dordrecht: Kluwer Academic Publishers
T2  - Plant Cell Tissue and Organ Culture
T1  - Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments
IS  - 2
VL  - 55
DO  - 10.1023/A:1006258426969
SP  - 109
EP  - 114
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Nešković, Mirjana",
year = "1999",
abstract = "Somatic embryogenesis was induced in embryogenic calli of Spinacia oleracea L., on a Murashige and Skoog (1962) medium, containing 4.6 μM kinetin as the sole growth regulator. Abscisic acid, gibberellic acid, and indole-3-acetic acid were supplemented to kinetin-containing medium and their effects on the initiation of somatic embryos was studied. Abscisic acid at a particular concentration (4 μM) dramatically increased the number of embryos per g fresh weight of callus, while both gibberellic acid and indole-3-acetic acid suppressed the embryo initiation. It is suggested that the promoting effect of abscisic acid on the embryo initiation may be explained as a stress response of the tissue. The relative number of globular embryos vs. the embryos in heart/torpedo and cotyledonary stages was increased at 4 μM abscisic acid and at all gibberellic acid concentrations (0.3–10 μM). In contrast, the ratio of globular to polar embryos was lower than in controls at 1 μM abscisic acid and indole-3-acetic acid (1 and 10 μM). The effects of growth regulators on the ratio of globular to polar embryos indicate that they interfere with the normal distribution of cell division and cell expansion during early embryogenesis.",
publisher = "Dordrecht: Kluwer Academic Publishers",
journal = "Plant Cell Tissue and Organ Culture",
title = "Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments",
number = "2",
volume = "55",
doi = "10.1023/A:1006258426969",
pages = "109-114"
}

Zdravković-Korać, S.,& Nešković, M.. (1999). Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments. in Plant Cell Tissue and Organ Culture
Dordrecht: Kluwer Academic Publishers., 55(2), 109-114.
https://doi.org/10.1023/A:1006258426969

Zdravković-Korać S, Nešković M. Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments. in Plant Cell Tissue and Organ Culture. 1999;55(2):109-114.
doi:10.1023/A:1006258426969 .

Zdravković-Korać, Snežana, Nešković, Mirjana, "Induction and development of somatic embryos from spinach (Spinacia oleracea) leaf segments" in Plant Cell Tissue and Organ Culture, 55, no. 2 (1999):109-114,
https://doi.org/10.1023/A:1006258426969 . .