TY  - JOUR
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.nature.com/articles/s41598-022-21413-y
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5084
AB  - Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.
PB  - Berlin: Nature Portfolio
T2  - Scientific Reports
T1  - Urban ecosystem drives genetic diversity in feral honey bee.
IS  - 1
VL  - 12
DO  - 10.1038/s41598-022-21413-y
SP  - 17692
ER  - 

@article{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihajlović, Milica and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.",
publisher = "Berlin: Nature Portfolio",
journal = "Scientific Reports",
title = "Urban ecosystem drives genetic diversity in feral honey bee.",
number = "1",
volume = "12",
doi = "10.1038/s41598-022-21413-y",
pages = "17692"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihajlović, M., Stanisavljević, L.,& Davidović, S.. (2022). Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports
Berlin: Nature Portfolio., 12(1), 17692.
https://doi.org/10.1038/s41598-022-21413-y

Patenković A, Tanasković M, Erić P, Erić K, Mihajlović M, Stanisavljević L, Davidović S. Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports. 2022;12(1):17692.
doi:10.1038/s41598-022-21413-y .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihajlović, Milica, Stanisavljević, Ljubiša, Davidović, Slobodan, "Urban ecosystem drives genetic diversity in feral honey bee." in Scientific Reports, 12, no. 1 (2022):17692,
https://doi.org/10.1038/s41598-022-21413-y . .

TY  - JOUR
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andrzej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4806
AB  - Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.
PB  - Basel: MDPI
T2  - Insects
T1  - Further Evidence of Population Admixture in the Serbian Honey Bee Population
IS  - 2
VL  - 13
DO  - 10.3390/insects13020180
SP  - 180
ER  - 

@article{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andrzej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Further Evidence of Population Admixture in the Serbian Honey Bee Population",
number = "2",
volume = "13",
doi = "10.3390/insects13020180",
pages = "180"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects
Basel: MDPI., 13(2), 180.
https://doi.org/10.3390/insects13020180

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects. 2022;13(2):180.
doi:10.3390/insects13020180 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andrzej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Further Evidence of Population Admixture in the Serbian Honey Bee Population" in Insects, 13, no. 2 (2022):180,
https://doi.org/10.3390/insects13020180 . .

TY  - CONF
AU  - Stanisavljević, Ljubiša
AU  - Bila-Dubaić, Jovana
AU  - SImonović, Slađan
AU  - Plećaš, Milan
AU  - Davidović, Slobodan
AU  - Tanasković, Marija
AU  - Ćetković, Aleksandar
PY  - 2022
UR  - http://www.parconazionalepantelleria.it/international_meeting_apis_silvatica.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5965
AB  - In the modern world managed bee species are necessary for the stability and sustainability of food production, of which the western honey bee (Apis mellifera) is the best known and the single most important species (Kevan, 2007). Wild honey bees have largely become extinct throughout Europe since the 1980s, following the introduction of the ectoparasitic mite Varroa destructor and various pathogens associated with it (Crane, 1999). The honey bee has been the subject of extensive research around the world for decades, particularly in terms of preserving the health and stability of their colonies under these new pressures. In that period, the importance of rare, accidentally surviving wild and feral colonies of honey bees was largely neglected. However, several recent studies (e.g., Le Conte et al., 2007) report on the growing number of colonies that have successfully survived the Varroa mite infestation without usual chemical treatments.
Unlike in other studies on unmanaged colonies, we presented a special case of the free-living population of honey bee in a large and highly populated urban environment of Belgrade, the capital of Serbia (Southeast Europe). During the period 2011–2017, we collected a large set of observational data (>1,300 records) from the apparently dense wild/feral honey bee population. Large share of these records can not be spatially related to the existence of managed apiaries, which are very rare in the core of downtown Belgrade. We hypothesized that numerous unmanaged bee colonies and the high frequency of swarms indicate a stable, self-sustaining wild population, and not the regular influx of swarms from the managed apiaries situated more peripherally. Also, we elaborated on various plausible explanations for this extraordinary finding.
By geospatial analysis of different categories of occurrence (colonies and swarms observed in different habitats and micro-situations) in relation to the parameters of urban environments suitability for honey bees, we evaluated the patterns of distribution and long-term survival of feral colonies under urban conditions. The results indicate an extremely high and stable population density and unusually long persistence of feral colonies, which is a rare phenomenon on a global scale (Bila Dubaić et al., 2021). Previous isolated cases of unmanaged colony survival (unknown in urban conditions) triggered great scientific attention: understanding the factors behind these survival cases may contribute to the efforts on improving the managed honey bee health. Therefore, our results contribute to ongoing initiatives to support naturally selected resistance mechanisms against Varroa mites, and hopefully reduce the currently growing incidence of colony die-offs.
The collecting of data was based on opportunistic citizen science, which we hereby evaluate as a highly prospective approach in the study of wild/feral bees in urban areas. We believe that specifically designed citizen science projects, based on our experiences, could be implemented with a great success in other countries with similar highly urbanised circumstances; in particular, investigations should be focused on environments with comparable potentials for the unnoticed existence of unmanaged honey bees (Bila Dubaić et al., 2021).
Despite the growing global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. In particular, we investigated and performed genetic analysis of 82 individual bee genomes in a portion of a sample of feral and managed honey bee colonies distributed throughout this large city, including highly urbanised areas. We established the existence of a high genetic differentiation between these two groups. Also, the comparison of mitochondrial and microsatellite DNA of bees sampled in Belgrade and in rural parts of Serbia showed that colonies of wild/feral bees have different patterns of genetic diversity. It can be concluded that urbanisation can be a positive driver of the genetic diversity of wild honey bees nesting in a highly urbanised and densely populated areas.
PB  - Isola di Pantelleria: Ente Parco Nazionale
C3  - First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy
T1  - Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5965
ER  - 

@conference{
author = "Stanisavljević, Ljubiša and Bila-Dubaić, Jovana and SImonović, Slađan and Plećaš, Milan and Davidović, Slobodan and Tanasković, Marija and Ćetković, Aleksandar",
year = "2022",
abstract = "In the modern world managed bee species are necessary for the stability and sustainability of food production, of which the western honey bee (Apis mellifera) is the best known and the single most important species (Kevan, 2007). Wild honey bees have largely become extinct throughout Europe since the 1980s, following the introduction of the ectoparasitic mite Varroa destructor and various pathogens associated with it (Crane, 1999). The honey bee has been the subject of extensive research around the world for decades, particularly in terms of preserving the health and stability of their colonies under these new pressures. In that period, the importance of rare, accidentally surviving wild and feral colonies of honey bees was largely neglected. However, several recent studies (e.g., Le Conte et al., 2007) report on the growing number of colonies that have successfully survived the Varroa mite infestation without usual chemical treatments.
Unlike in other studies on unmanaged colonies, we presented a special case of the free-living population of honey bee in a large and highly populated urban environment of Belgrade, the capital of Serbia (Southeast Europe). During the period 2011–2017, we collected a large set of observational data (>1,300 records) from the apparently dense wild/feral honey bee population. Large share of these records can not be spatially related to the existence of managed apiaries, which are very rare in the core of downtown Belgrade. We hypothesized that numerous unmanaged bee colonies and the high frequency of swarms indicate a stable, self-sustaining wild population, and not the regular influx of swarms from the managed apiaries situated more peripherally. Also, we elaborated on various plausible explanations for this extraordinary finding.
By geospatial analysis of different categories of occurrence (colonies and swarms observed in different habitats and micro-situations) in relation to the parameters of urban environments suitability for honey bees, we evaluated the patterns of distribution and long-term survival of feral colonies under urban conditions. The results indicate an extremely high and stable population density and unusually long persistence of feral colonies, which is a rare phenomenon on a global scale (Bila Dubaić et al., 2021). Previous isolated cases of unmanaged colony survival (unknown in urban conditions) triggered great scientific attention: understanding the factors behind these survival cases may contribute to the efforts on improving the managed honey bee health. Therefore, our results contribute to ongoing initiatives to support naturally selected resistance mechanisms against Varroa mites, and hopefully reduce the currently growing incidence of colony die-offs.
The collecting of data was based on opportunistic citizen science, which we hereby evaluate as a highly prospective approach in the study of wild/feral bees in urban areas. We believe that specifically designed citizen science projects, based on our experiences, could be implemented with a great success in other countries with similar highly urbanised circumstances; in particular, investigations should be focused on environments with comparable potentials for the unnoticed existence of unmanaged honey bees (Bila Dubaić et al., 2021).
Despite the growing global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. In particular, we investigated and performed genetic analysis of 82 individual bee genomes in a portion of a sample of feral and managed honey bee colonies distributed throughout this large city, including highly urbanised areas. We established the existence of a high genetic differentiation between these two groups. Also, the comparison of mitochondrial and microsatellite DNA of bees sampled in Belgrade and in rural parts of Serbia showed that colonies of wild/feral bees have different patterns of genetic diversity. It can be concluded that urbanisation can be a positive driver of the genetic diversity of wild honey bees nesting in a highly urbanised and densely populated areas.",
publisher = "Isola di Pantelleria: Ente Parco Nazionale",
journal = "First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy",
title = "Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5965"
}

Stanisavljević, L., Bila-Dubaić, J., SImonović, S., Plećaš, M., Davidović, S., Tanasković, M.,& Ćetković, A.. (2022). Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia). in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy
Isola di Pantelleria: Ente Parco Nazionale..
https://hdl.handle.net/21.15107/rcub_ibiss_5965

Stanisavljević L, Bila-Dubaić J, SImonović S, Plećaš M, Davidović S, Tanasković M, Ćetković A. Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia). in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5965 .

Stanisavljević, Ljubiša, Bila-Dubaić, Jovana, SImonović, Slađan, Plećaš, Milan, Davidović, Slobodan, Tanasković, Marija, Ćetković, Aleksandar, "Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)" in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5965 .

TY  - DATA
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihailović, Milica
AU  - Tanasić, Vanja
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4954
AB  - Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.
T1  - Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4954
ER  - 

@misc{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihailović, Milica and Tanasić, Vanja and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.",
title = "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4954"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihailović, M., Tanasić, V., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". .
https://hdl.handle.net/21.15107/rcub_ibiss_4954

Patenković A, Tanasković M, Erić P, Erić K, Mihailović M, Tanasić V, Stanisavljević L, Davidović S. Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihailović, Milica, Tanasić, Vanja, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"" (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

TY  - CONF
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5039
AB  - Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Medonosne pčele Srbije – populaciono-genetički pogled
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5039
ER  - 

@conference{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Medonosne pčele Srbije – populaciono-genetički pogled",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5039"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039

Patenković A, Tanasković M, Erić P, Erić K, Stanisavljević L, Davidović S. Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Medonosne pčele Srbije – populaciono-genetički pogled" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):87,
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5037
AB  - Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
T1  - Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5037
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Patenković, Aleksandra and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi",
title = "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5037"
}

Erić, P., Tanasković, M., Patenković, A., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
Belgrade : Entomological Society of Serbia., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037

Erić P, Tanasković M, Patenković A, Erić K, Stanisavljević L, Davidović S. Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

Erić, Pavle, Tanasković, Marija, Patenković, Aleksandra, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5035
AB  - The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.
PB  - European Society for Evolutionary Biology
C3  - Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny
SP  - 753
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5035
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Erić, Katarina and Patenković, Aleksandra and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.",
publisher = "European Society for Evolutionary Biology",
journal = "Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny",
pages = "753",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5035"
}

Erić, P., Tanasković, M., Erić, K., Patenković, A., Stanisavljević, L.,& Davidović, S.. (2022). Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035

Erić P, Tanasković M, Erić K, Patenković A, Stanisavljević L, Davidović S. Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

Erić, Pavle, Tanasković, Marija, Erić, Katarina, Patenković, Aleksandra, Stanisavljević, Ljubiša, Davidović, Slobodan, "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny" in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):753,
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - https://eurbee9.bio.bg.ac.rs/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5038
AB  - The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.
PB  - European Association for Bee Research
C3  - Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
T1  - Free-living vs. managed honey bees – a population genetic approach
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5038
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.",
publisher = "European Association for Bee Research",
journal = "Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia",
title = "Free-living vs. managed honey bees – a population genetic approach",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5038"
}

Davidović, S., Patenković, A., Erić, K., Erić, P., Stanisavljević, L.,& Tanasković, M.. (2022). Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
European Association for Bee Research., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038

Davidović S, Patenković A, Erić K, Erić P, Stanisavljević L, Tanasković M. Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Stanisavljević, Ljubiša, Tanasković, Marija, "Free-living vs. managed honey bees – a population genetic approach" in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5036
AB  - Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
T1  - Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5036
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.",
title = "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5036"
}

Davidović, S., Patenković, A., Erić, P., Erić, K., Stanisavljević, L.,& Tanasković, M.. (2022). Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
Belgrade : Entomological Society of Serbia., 47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036

Davidović S, Patenković A, Erić P, Erić K, Stanisavljević L, Tanasković M. Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.. 2022;:47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Tanasković, Marija, "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia. (2022):47-48,
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

TY  - DATA
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andzrej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4779
AB  - Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.
T2  - Insects
T1  - Supplementary material "Further evidence of population admixture in the Serbian honey bee population"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4779
ER  - 

@misc{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andzrej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.",
journal = "Insects",
title = "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4779"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects.
https://hdl.handle.net/21.15107/rcub_ibiss_4779

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andzrej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"" in Insects (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

TY  - JOUR
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4429
AB  - Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise Fst values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.
PB  - Basel: MDPI
T2  - Insects
T1  - MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity
IS  - 9
VL  - 12
DO  - 10.3390/insects12090767
SP  - 767
ER  - 

@article{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise Fst values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity",
number = "9",
volume = "12",
doi = "10.3390/insects12090767",
pages = "767"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Stanisavljević, L.,& Davidović, S.. (2021). MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity. in Insects
Basel: MDPI., 12(9), 767.
https://doi.org/10.3390/insects12090767

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Stanisavljević L, Davidović S. MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity. in Insects. 2021;12(9):767.
doi:10.3390/insects12090767 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Stanisavljević, Ljubiša, Davidović, Slobodan, "MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity" in Insects, 12, no. 9 (2021):767,
https://doi.org/10.3390/insects12090767 . .

TY  - JOUR
AU  - Bila Dubaić, Jovana
AU  - Simonović, Slađan
AU  - Plećaš, Milan
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
AU  - Tanasković, Marija
AU  - Ćetković, Aleksandar
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4708
AB  - It is assumed that wild honey bees have become largely extinct across Europe since the 1980s, following the introduction of exotic ectoparasitic mite (Varroa) and the associated spillover of various pathogens. However, several recent studies reported on unmanaged colonies that survived the Varroa mite infestation. Herewith, we present another case of unmanaged, free-living population of honey bees in SE Europe, a rare case of feral bees inhabiting a large and highly populated urban area: Belgrade, the capital of Serbia. We compiled a massive data-set derived from opportunistic citizen science (>1300 records) during the 2011–2017 period and investigated whether these honey bee colonies and the high incidence of swarms could be a result of a stable, self-sustaining feral population (i.e., not of regular inflow of swarms escaping from local managed apiaries), and discussed various explanations for its existence. We also present the possibilities and challenges associated with the detection and effective monitoring of feral/wild honey bees in urban settings, and the role of citizen science in such endeavors. Our results will underpin ongoing initiatives to better understand and support naturally selected resistance mechanisms against the Varroa mite, which should contribute to alleviating current threats and risks to global apiculture and food production security.
PB  - Basel: MDPI
T2  - Insects
T1  - Unprecedented Density and Persistence of Feral Honey Bees in Urban Environments of a Large SE-European City (Belgrade, Serbia)
IS  - 12
VL  - 12
DO  - 10.3390/insects12121127
SP  - 1127
ER  - 

@article{
author = "Bila Dubaić, Jovana and Simonović, Slađan and Plećaš, Milan and Stanisavljević, Ljubiša and Davidović, Slobodan and Tanasković, Marija and Ćetković, Aleksandar",
year = "2021",
abstract = "It is assumed that wild honey bees have become largely extinct across Europe since the 1980s, following the introduction of exotic ectoparasitic mite (Varroa) and the associated spillover of various pathogens. However, several recent studies reported on unmanaged colonies that survived the Varroa mite infestation. Herewith, we present another case of unmanaged, free-living population of honey bees in SE Europe, a rare case of feral bees inhabiting a large and highly populated urban area: Belgrade, the capital of Serbia. We compiled a massive data-set derived from opportunistic citizen science (>1300 records) during the 2011–2017 period and investigated whether these honey bee colonies and the high incidence of swarms could be a result of a stable, self-sustaining feral population (i.e., not of regular inflow of swarms escaping from local managed apiaries), and discussed various explanations for its existence. We also present the possibilities and challenges associated with the detection and effective monitoring of feral/wild honey bees in urban settings, and the role of citizen science in such endeavors. Our results will underpin ongoing initiatives to better understand and support naturally selected resistance mechanisms against the Varroa mite, which should contribute to alleviating current threats and risks to global apiculture and food production security.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Unprecedented Density and Persistence of Feral Honey Bees in Urban Environments of a Large SE-European City (Belgrade, Serbia)",
number = "12",
volume = "12",
doi = "10.3390/insects12121127",
pages = "1127"
}

Bila Dubaić, J., Simonović, S., Plećaš, M., Stanisavljević, L., Davidović, S., Tanasković, M.,& Ćetković, A.. (2021). Unprecedented Density and Persistence of Feral Honey Bees in Urban Environments of a Large SE-European City (Belgrade, Serbia). in Insects
Basel: MDPI., 12(12), 1127.
https://doi.org/10.3390/insects12121127

Bila Dubaić J, Simonović S, Plećaš M, Stanisavljević L, Davidović S, Tanasković M, Ćetković A. Unprecedented Density and Persistence of Feral Honey Bees in Urban Environments of a Large SE-European City (Belgrade, Serbia). in Insects. 2021;12(12):1127.
doi:10.3390/insects12121127 .

Bila Dubaić, Jovana, Simonović, Slađan, Plećaš, Milan, Stanisavljević, Ljubiša, Davidović, Slobodan, Tanasković, Marija, Ćetković, Aleksandar, "Unprecedented Density and Persistence of Feral Honey Bees in Urban Environments of a Large SE-European City (Belgrade, Serbia)" in Insects, 12, no. 12 (2021):1127,
https://doi.org/10.3390/insects12121127 . .

TY  - CONF
AU  - Tanasković, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://sciforum.net/paper/view/10720
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4469
AB  - Practice of commercial honey bee breeding and selection for desired traits, intensification of queen importation and migration of once stationary apiaries significantly influences distribution and genetic diversity of local subspecies, populations and ecotypes. Bee colonies worldwide are facing serious declines resulting in colonies loss and reduction of genetic diversity. Thus, reassessing the genetic status of native honey bee populations becomes imperative. The latest reports, which include samples from nine years ago, suggest the presence of both Apis mellifera carnica in north and A. m. macedonica in the south of Serbia and significant hybridization between two subspecies.
To assess genetic diversity of contemporary managed honey bee colonies we used 14 microsatellite loci and analyzed 227 worker bees from 46 apiaries in 8 localities from northern and southern Serbia. RFLP analysis on the COI gene segment of mtDNA was used to distinguish A. m. carnica from A. m. macedonica. Mean number of alleles ranged from 5.14 to 9.00, observed heterozygosity from 0.43 to 0.56 and STRUCTURE analysis showed existence of three distinct genetic clusters. DAPC analysis showed huge overlapping of individuals from different parts of Serbia with weak clustering according to geographical origin of three groups. RFLP analysis showed the presence of A. m. carnica subspecies only.
Absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia as well as lack of distinctive geographical clusters suggest that selective breeding, queen import and migratory beekeeping practices strongly influenced genetic structure and diversity of honey bees leading to the genetic uniformisation and absence of locally adapted populations.
PB  - Basel: MDPI
C3  - The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15
T1  - Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia
DO  - 10.3390/IECE-10720
ER  - 

@conference{
author = "Tanasković, Marija and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Practice of commercial honey bee breeding and selection for desired traits, intensification of queen importation and migration of once stationary apiaries significantly influences distribution and genetic diversity of local subspecies, populations and ecotypes. Bee colonies worldwide are facing serious declines resulting in colonies loss and reduction of genetic diversity. Thus, reassessing the genetic status of native honey bee populations becomes imperative. The latest reports, which include samples from nine years ago, suggest the presence of both Apis mellifera carnica in north and A. m. macedonica in the south of Serbia and significant hybridization between two subspecies.
To assess genetic diversity of contemporary managed honey bee colonies we used 14 microsatellite loci and analyzed 227 worker bees from 46 apiaries in 8 localities from northern and southern Serbia. RFLP analysis on the COI gene segment of mtDNA was used to distinguish A. m. carnica from A. m. macedonica. Mean number of alleles ranged from 5.14 to 9.00, observed heterozygosity from 0.43 to 0.56 and STRUCTURE analysis showed existence of three distinct genetic clusters. DAPC analysis showed huge overlapping of individuals from different parts of Serbia with weak clustering according to geographical origin of three groups. RFLP analysis showed the presence of A. m. carnica subspecies only.
Absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia as well as lack of distinctive geographical clusters suggest that selective breeding, queen import and migratory beekeeping practices strongly influenced genetic structure and diversity of honey bees leading to the genetic uniformisation and absence of locally adapted populations.",
publisher = "Basel: MDPI",
journal = "The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15",
title = "Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia",
doi = "10.3390/IECE-10720"
}

Tanasković, M., Patenković, A., Erić, K., Erić, P., Stanisavljević, L.,& Davidović, S.. (2021). Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia. in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15
Basel: MDPI..
https://doi.org/10.3390/IECE-10720

Tanasković M, Patenković A, Erić K, Erić P, Stanisavljević L, Davidović S. Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia. in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15. 2021;.
doi:10.3390/IECE-10720 .

Tanasković, Marija, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Stanisavljević, Ljubiša, Davidović, Slobodan, "Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia" in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15 (2021),
https://doi.org/10.3390/IECE-10720 . .

TY  - CONF
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasić, Vanja
AU  - Mihajlović, Milica
AU  - Tanasković, Marija
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4253
AB  - Abstract
Local populations of Apis mellifera are rapidly changing, with the gene pool of autochthonous breeds being depleted by beekeepers through the import of foreign queens not adapted to the local environment. To study their genetic structure and phylogenetic relationships, we gathered a large dataset from the Balkans and surrounding countries.
Our sample consisted of 246 honeybee colonies collected from 47 apiaries and 24 feral colonies divided into four subpopulations from southern Serbia, five from Vojvodina, and two from Belgrade. To evaluate genetic diversity patterns, we sequenced the mitochondrial tRNAleu-cox2 intergenic region. We compared our data to other published data on A.mellifera COI-COII intergenic region variability in the Balkans and neighboring countries. We pulled 1512 sequences from the NCBI GenBank, originating from 15 different populations. The 1782 mitochondrial sequences were grouped into 31 haplotypes, with two newly described haplotypes from our sample. All haplotypes belonged to the eastern Mediterranean C lineage. The most frequent haplotype was C2d, characteristic for A.m.macedonica, followed by C2c and C1a characteristic for A.m.carnica and A.m.ligustica respectively. In our samples 9 haplotypes were observed, with the C2d being the most common and widespread as it was detected in all 11 groups, followed by C2e that was detected in all but one group. C2c and C1a were a little less common than in the total sample but very widespread as they were present in seven groups.
When the Macedonia, Ukraine, and Belgrade honeybee populations which consisted of a single haplotype, were excluded, the haplotype diversity ranged from 0.0998 to 0.7477, nucleotide diversity ranged from the lowest value of 0.000114 to 0.003731. The mean number of pairwise differences for populations that had more than one haplotype ranged from 0.060577 to 2. MDS plot constructed on pairwise FST values shows significant geographical stratification, with our subpopulations being grouped together. Vojvodina being placed closer to Romania and Hungary datasets, while southern Serbia is closer to Bulgaria and Montenegro. Interestingly, our samples are not closely grouped with the Serbian dataset from the GenBank which indicates that honeybee populations are changing rapidly.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective
IS  - 1
VL  - 43
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4253
ER  - 

@conference{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasić, Vanja and Mihajlović, Milica and Tanasković, Marija and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Abstract
Local populations of Apis mellifera are rapidly changing, with the gene pool of autochthonous breeds being depleted by beekeepers through the import of foreign queens not adapted to the local environment. To study their genetic structure and phylogenetic relationships, we gathered a large dataset from the Balkans and surrounding countries.
Our sample consisted of 246 honeybee colonies collected from 47 apiaries and 24 feral colonies divided into four subpopulations from southern Serbia, five from Vojvodina, and two from Belgrade. To evaluate genetic diversity patterns, we sequenced the mitochondrial tRNAleu-cox2 intergenic region. We compared our data to other published data on A.mellifera COI-COII intergenic region variability in the Balkans and neighboring countries. We pulled 1512 sequences from the NCBI GenBank, originating from 15 different populations. The 1782 mitochondrial sequences were grouped into 31 haplotypes, with two newly described haplotypes from our sample. All haplotypes belonged to the eastern Mediterranean C lineage. The most frequent haplotype was C2d, characteristic for A.m.macedonica, followed by C2c and C1a characteristic for A.m.carnica and A.m.ligustica respectively. In our samples 9 haplotypes were observed, with the C2d being the most common and widespread as it was detected in all 11 groups, followed by C2e that was detected in all but one group. C2c and C1a were a little less common than in the total sample but very widespread as they were present in seven groups.
When the Macedonia, Ukraine, and Belgrade honeybee populations which consisted of a single haplotype, were excluded, the haplotype diversity ranged from 0.0998 to 0.7477, nucleotide diversity ranged from the lowest value of 0.000114 to 0.003731. The mean number of pairwise differences for populations that had more than one haplotype ranged from 0.060577 to 2. MDS plot constructed on pairwise FST values shows significant geographical stratification, with our subpopulations being grouped together. Vojvodina being placed closer to Romania and Hungary datasets, while southern Serbia is closer to Bulgaria and Montenegro. Interestingly, our samples are not closely grouped with the Serbian dataset from the GenBank which indicates that honeybee populations are changing rapidly.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective",
number = "1",
volume = "43",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4253"
}

Erić, P., Patenković, A., Erić, K., Tanasić, V., Mihajlović, M., Tanasković, M., Stanisavljević, L.,& Davidović, S.. (2021). Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 116.
https://hdl.handle.net/21.15107/rcub_ibiss_4253

Erić P, Patenković A, Erić K, Tanasić V, Mihajlović M, Tanasković M, Stanisavljević L, Davidović S. Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):116.
https://hdl.handle.net/21.15107/rcub_ibiss_4253 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasić, Vanja, Mihajlović, Milica, Tanasković, Marija, Stanisavljević, Ljubiša, Davidović, Slobodan, "Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):116,
https://hdl.handle.net/21.15107/rcub_ibiss_4253 .