TY  - CONF
AU  - Drača, Dijana
AU  - Mijatović, Sanja
AU  - Krajnović, Tamara
AU  - Bogdanović Pristov, Jelena
AU  - Đukić, Tatjana
AU  - Kaluđerović, Goran N.
AU  - Wessjohann, Ludger A.
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5292
AB  - Tubulisins are natural peptide compounds isolated from mycobacterial genera.
They belong to the group of antimitotic agents, since they achieve their antitumor
effect disrupting the function of mitotic spindle. The object of this study was to investigate
anticancer potential of synthetic analogue of tubulysins, tubugi 1, on mouse
melanoma model, in vitro and in vivo. Tubugi 1 decreased dose-dependently viability
of B16 cells. The experimental compound induced atypical apoptosis without the externalization
of phosphatidylserines (PS). Although apoptosis was accompanied with
strong intracellular production of reactive oxygen and nitrogen species, decrease in
malonyldyaldehyde content showed that membrane lipids were not subjected to oxidation,
what is a prerequisite for the externalization of PS. Although PS plays a key
role in the removal of apoptotic cells, this did not affect the phagocytic activity of
macrophages in vitro, implying PS-independent apoptotic cells removal. The effect of
the experimental agent was confirmed in vivo. Мacrophages isolated from peritoneal
exudate of treated animals showed cytotoxic activity, what was in line with demonstrated
expression of M1 phenotype markers, as well as production of nitric oxide.
Additonally, the phagocytic activity of these cells was preserved. Having in mind lack
of data in the literature concerning the effects of this group of agents on components
of the innate immune system, tubugi 1 remains worthy of further research in the field
of experimental oncology.
AB  - Тубулизини су природна једињења изолована из родова миксобактерија.
Спадају у групу антимитотских агенаса будући да свој антитуморски ефекат
остварују на нивоу деобног вретена. У овој студији испитиван је антитумор-
ски потенцијал синтетског аналога тубулизина, тубуги 1, in vitro и in vivo на
моделу мишјег меланома. Тубуги 1 је на дозно-зависан начин смањио вијаби-
литет B16 ћелија. Експериментално једињење је у овим ћелијама индуковало
атипичну форму апоптотске ћелијске смрти без екстернализације фосфати-
дилсерина (ПС). Иако је апоптоза била праћена снажном продукцијом ре-
активних врста кисеоника и азота, смањење садржаја малонилдиалдехида је
показало да мембрански липиди нису подлегли оксидацији, што је предуслов
за екстернализацију ПС. Иако ПС има кључну улогу у уклањању апоптотских
ћелија, ово се није одразило на фагоцитну активност макрофага in vitro, ука-
зујући на фагоцитозу независну од ПС. Учинак експерименталног агенса је
потврђен in vivo. Макрофаги изоловани из перитонеалног ексудата третира-
них животиња показали су цитотоксичну активност, што је у сагласности са
показаном експресијом маркера М1 фенотипа и продукцијом азот моноксида.
Додатно, фагоцитна способност ових ћелија је била очувана. С обзиром на
недостатак података у литератури о деловању овакве групе агенаса на компо-
ненте урођеног имунског система, тубуги 1 остаје вредан даљих испитивања
на пољу експерименталне онкологије.
PB  - Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
C3  - Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
T1  - New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model
SP  - 41
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5292
ER  - 

@conference{
author = "Drača, Dijana and Mijatović, Sanja and Krajnović, Tamara and Bogdanović Pristov, Jelena and Đukić, Tatjana and Kaluđerović, Goran N. and Wessjohann, Ludger A. and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Tubulisins are natural peptide compounds isolated from mycobacterial genera.
They belong to the group of antimitotic agents, since they achieve their antitumor
effect disrupting the function of mitotic spindle. The object of this study was to investigate
anticancer potential of synthetic analogue of tubulysins, tubugi 1, on mouse
melanoma model, in vitro and in vivo. Tubugi 1 decreased dose-dependently viability
of B16 cells. The experimental compound induced atypical apoptosis without the externalization
of phosphatidylserines (PS). Although apoptosis was accompanied with
strong intracellular production of reactive oxygen and nitrogen species, decrease in
malonyldyaldehyde content showed that membrane lipids were not subjected to oxidation,
what is a prerequisite for the externalization of PS. Although PS plays a key
role in the removal of apoptotic cells, this did not affect the phagocytic activity of
macrophages in vitro, implying PS-independent apoptotic cells removal. The effect of
the experimental agent was confirmed in vivo. Мacrophages isolated from peritoneal
exudate of treated animals showed cytotoxic activity, what was in line with demonstrated
expression of M1 phenotype markers, as well as production of nitric oxide.
Additonally, the phagocytic activity of these cells was preserved. Having in mind lack
of data in the literature concerning the effects of this group of agents on components
of the innate immune system, tubugi 1 remains worthy of further research in the field
of experimental oncology., Тубулизини су природна једињења изолована из родова миксобактерија.
Спадају у групу антимитотских агенаса будући да свој антитуморски ефекат
остварују на нивоу деобног вретена. У овој студији испитиван је антитумор-
ски потенцијал синтетског аналога тубулизина, тубуги 1, in vitro и in vivo на
моделу мишјег меланома. Тубуги 1 је на дозно-зависан начин смањио вијаби-
литет B16 ћелија. Експериментално једињење је у овим ћелијама индуковало
атипичну форму апоптотске ћелијске смрти без екстернализације фосфати-
дилсерина (ПС). Иако је апоптоза била праћена снажном продукцијом ре-
активних врста кисеоника и азота, смањење садржаја малонилдиалдехида је
показало да мембрански липиди нису подлегли оксидацији, што је предуслов
за екстернализацију ПС. Иако ПС има кључну улогу у уклањању апоптотских
ћелија, ово се није одразило на фагоцитну активност макрофага in vitro, ука-
зујући на фагоцитозу независну од ПС. Учинак експерименталног агенса је
потврђен in vivo. Макрофаги изоловани из перитонеалног ексудата третира-
них животиња показали су цитотоксичну активност, што је у сагласности са
показаном експресијом маркера М1 фенотипа и продукцијом азот моноксида.
Додатно, фагоцитна способност ових ћелија је била очувана. С обзиром на
недостатак података у литератури о деловању овакве групе агенаса на компо-
ненте урођеног имунског система, тубуги 1 остаје вредан даљих испитивања
на пољу експерименталне онкологије.",
publisher = "Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine",
journal = "Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina",
title = "New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model",
pages = "41-42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5292"
}

Drača, D., Mijatović, S., Krajnović, T., Bogdanović Pristov, J., Đukić, T., Kaluđerović, G. N., Wessjohann, L. A.,& Maksimović-Ivanić, D.. (2022). New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5292

Drača D, Mijatović S, Krajnović T, Bogdanović Pristov J, Đukić T, Kaluđerović GN, Wessjohann LA, Maksimović-Ivanić D. New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. 2022;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5292 .

Drača, Dijana, Mijatović, Sanja, Krajnović, Tamara, Bogdanović Pristov, Jelena, Đukić, Tatjana, Kaluđerović, Goran N., Wessjohann, Ludger A., Maksimović-Ivanić, Danijela, "New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model" in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina (2022):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5292 .

TY  - CONF
AU  - Krajnović, Tamara
AU  - Drača, Dijana
AU  - Kaluđerović, Goran N.
AU  - Dunđerović, Duško
AU  - Mirkov, Ivana
AU  - Wessjohann, Ludger A.
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5291
AB  - A prenylflavonoid from hops, isoxanthohumol (IXN) is gaining a lot of attention
nowadays due to its broad spectrum of biological activities. This study aimed to investigate
its potential antimetastatic properties in vitro against the highly invasive melanoma
cell line B16-F10 and in a murine metastatic model in vivo, in the context of
independent action and interaction with chemotherapy. The treatment with IXN diminished
cell viability in a dose-dependent manner, which is a consequence of induced
combined cell death by autophagy and caspase-dependent apoptosis. Additionally, the
dividing potential of highly proliferative melanoma cells was dramatically affected by
IXN, and is in line with an abrogated clonogenic potential in the population of survived
cells, indicating changes in their metastatic features. Concordantly, IXN strongly diminished
cell metastatic features down-regulating adhesion, migration, and invasion
through disrupted integrin signaling. Separate application of IXN inhibited the development
of lung metastatic foci in tumor-challenged animals. Although IXN potentiated
the action of chemotherapeutic drug paclitaxel (PCT) in vitro, the concomitant
treatment of these two agents did not exert statistically significant suppression of lung
metastases formation. Nonetheless, the change in the regime of drugs’ application to
7-day treatment with IXN prior to PCT made the subtherapeutic dose of PCT effective,
indicating IXNs’ capacity to trigger the differentiation of B16-F10 cells toward a less
invasive phenotype, thus sensitizing them to chemotherapy. Along with histopathological
confirmation of changes in cell phenotype in metastases, this data underlines the
important impact of the differentiation process on chemotherapy efficacy. This study
represents the first proof of IXNs’ antimetastatic activity in vivo and significantly contributes
to the development of the differentiation therapy concept as a support in the
treatment of metastatic malignancies.
AB  - Пренилфлавоноид из хмеља, изоксантохумол (IXN) данас привлачи велику
пажњу због свог широког спектра биолошких активности. Ова студија је имала
за циљ да истражи његова потенцијална антиметастатска својства на високо ин-
вазивној ћелијској линији меланома B16-F10 in vitro и in vivo, у контексту њего-
вог самосталног деловања или у комбинацији са хемотерапијом. Третман IXN је
смањио вијабилитет ћелија меланома на дозно зависан начин, што је последица
индуковане комбиноване ћелијске смрти аутофагијом и апоптозом зависном од
каспаза. Поред тога, овај изофлаванон је снажно инхибирао деобу високо про-
лиферативних ћелија меланома као и клоногени потенцијал преживелих ћели-
ја, указујући на промене у њиховим метастатским карактеристикама. Сагласно
томе, IXN је супримирао процесе који дефинишу метастазирање као што су
ћелијска адхезија, миграција и инвазија, ометајући интегрински сигнални пут.
Примена IXN је инхибирала развој метастатских жаришта у плућима експери-
менталних животиња. Иако је IXN појачао дејство хемотерапеутика паклитаксе-
ла (PCT) in vitro, истовремени третман ова два агенса није довео до статистички
значајне супресије у формирању плућних метастаза. Са друге стране, промена
динамике давања агенаса у виду 7-дневног третмана животиња IXN пре апли-
кације PCT учинила је ефикасном субтерапеутску дозу PCT, што се може при-
писати његовом капацитету да диферентује B16-F10 ћелије ка мање инвазивном
фенотипу и тиме их сензитизује на хемотерапију. Уз хистопатолошку потврду
промене фенотипа ћелија у метастазама, овај податак указује на значајан до-
принос процеса диференцијације успешности исхода хемотерапије. Ова студија
представља први доказ антиметастатске активности IXN in vivo и значајно до-
приноси успостављању концепта диференцијационе терапије у подршци лечењу
метастатских малигнитета.
PB  - Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
C3  - Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
T1  - Antimetastatic effect of prenylfavonoid isoxanthohumol on b16-f10 murine melanoma model
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5291
ER  - 

@conference{
author = "Krajnović, Tamara and Drača, Dijana and Kaluđerović, Goran N. and Dunđerović, Duško and Mirkov, Ivana and Wessjohann, Ludger A. and Maksimović-Ivanić, Danijela and Mijatović, Sanja",
year = "2022",
abstract = "A prenylflavonoid from hops, isoxanthohumol (IXN) is gaining a lot of attention
nowadays due to its broad spectrum of biological activities. This study aimed to investigate
its potential antimetastatic properties in vitro against the highly invasive melanoma
cell line B16-F10 and in a murine metastatic model in vivo, in the context of
independent action and interaction with chemotherapy. The treatment with IXN diminished
cell viability in a dose-dependent manner, which is a consequence of induced
combined cell death by autophagy and caspase-dependent apoptosis. Additionally, the
dividing potential of highly proliferative melanoma cells was dramatically affected by
IXN, and is in line with an abrogated clonogenic potential in the population of survived
cells, indicating changes in their metastatic features. Concordantly, IXN strongly diminished
cell metastatic features down-regulating adhesion, migration, and invasion
through disrupted integrin signaling. Separate application of IXN inhibited the development
of lung metastatic foci in tumor-challenged animals. Although IXN potentiated
the action of chemotherapeutic drug paclitaxel (PCT) in vitro, the concomitant
treatment of these two agents did not exert statistically significant suppression of lung
metastases formation. Nonetheless, the change in the regime of drugs’ application to
7-day treatment with IXN prior to PCT made the subtherapeutic dose of PCT effective,
indicating IXNs’ capacity to trigger the differentiation of B16-F10 cells toward a less
invasive phenotype, thus sensitizing them to chemotherapy. Along with histopathological
confirmation of changes in cell phenotype in metastases, this data underlines the
important impact of the differentiation process on chemotherapy efficacy. This study
represents the first proof of IXNs’ antimetastatic activity in vivo and significantly contributes
to the development of the differentiation therapy concept as a support in the
treatment of metastatic malignancies., Пренилфлавоноид из хмеља, изоксантохумол (IXN) данас привлачи велику
пажњу због свог широког спектра биолошких активности. Ова студија је имала
за циљ да истражи његова потенцијална антиметастатска својства на високо ин-
вазивној ћелијској линији меланома B16-F10 in vitro и in vivo, у контексту њего-
вог самосталног деловања или у комбинацији са хемотерапијом. Третман IXN је
смањио вијабилитет ћелија меланома на дозно зависан начин, што је последица
индуковане комбиноване ћелијске смрти аутофагијом и апоптозом зависном од
каспаза. Поред тога, овај изофлаванон је снажно инхибирао деобу високо про-
лиферативних ћелија меланома као и клоногени потенцијал преживелих ћели-
ја, указујући на промене у њиховим метастатским карактеристикама. Сагласно
томе, IXN је супримирао процесе који дефинишу метастазирање као што су
ћелијска адхезија, миграција и инвазија, ометајући интегрински сигнални пут.
Примена IXN је инхибирала развој метастатских жаришта у плућима експери-
менталних животиња. Иако је IXN појачао дејство хемотерапеутика паклитаксе-
ла (PCT) in vitro, истовремени третман ова два агенса није довео до статистички
значајне супресије у формирању плућних метастаза. Са друге стране, промена
динамике давања агенаса у виду 7-дневног третмана животиња IXN пре апли-
кације PCT учинила је ефикасном субтерапеутску дозу PCT, што се може при-
писати његовом капацитету да диферентује B16-F10 ћелије ка мање инвазивном
фенотипу и тиме их сензитизује на хемотерапију. Уз хистопатолошку потврду
промене фенотипа ћелија у метастазама, овај податак указује на значајан до-
принос процеса диференцијације успешности исхода хемотерапије. Ова студија
представља први доказ антиметастатске активности IXN in vivo и значајно до-
приноси успостављању концепта диференцијационе терапије у подршци лечењу
метастатских малигнитета.",
publisher = "Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine",
journal = "Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina",
title = "Antimetastatic effect of prenylfavonoid isoxanthohumol on b16-f10 murine melanoma model",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5291"
}

Krajnović, T., Drača, D., Kaluđerović, G. N., Dunđerović, D., Mirkov, I., Wessjohann, L. A., Maksimović-Ivanić, D.,& Mijatović, S.. (2022). Antimetastatic effect of prenylfavonoid isoxanthohumol on b16-f10 murine melanoma model. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine., 152-153.
https://hdl.handle.net/21.15107/rcub_ibiss_5291

Krajnović T, Drača D, Kaluđerović GN, Dunđerović D, Mirkov I, Wessjohann LA, Maksimović-Ivanić D, Mijatović S. Antimetastatic effect of prenylfavonoid isoxanthohumol on b16-f10 murine melanoma model. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. 2022;:152-153.
https://hdl.handle.net/21.15107/rcub_ibiss_5291 .

Krajnović, Tamara, Drača, Dijana, Kaluđerović, Goran N., Dunđerović, Duško, Mirkov, Ivana, Wessjohann, Ludger A., Maksimović-Ivanić, Danijela, Mijatović, Sanja, "Antimetastatic effect of prenylfavonoid isoxanthohumol on b16-f10 murine melanoma model" in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina (2022):152-153,
https://hdl.handle.net/21.15107/rcub_ibiss_5291 .

TY  - JOUR
AU  - Stanković, Jelena
AU  - Raković, Maja
AU  - Paunović, Momir
AU  - Atanacković, Ana
AU  - Tomović, Jelena
AU  - Milošević, Đurađ
PY  - 2021
UR  - http://casopisi.junis.ni.ac.rs/index.php/FUMedBiol/article/view/10165
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4890
AB  - The study was conducted on the Danube River, within the project Joint Danube Survey 3 (JDS3). The main aim was to estimate the quantity of microplastics in aquatic ecosystems through passive biological monitoring. Three freshwater species were used for microplastic (MP) isolation from different taxonomic groups of organisms: Mollusca, Oligochaeta, and Chironomidae (Diptera), with the following species: Lithoglyphus naticoides (C. Pfeiffer, 1828), Limnodrilus hoffmeisteri (Claparede, 1862), and Chironomus acutiventris (Wülker, Ryser & Scholl, 1983), respectively. The samples were collected from 6 sites along the Danube River where 540 specimens were examined. The samples were digested by alkaline method (incubation in 10% KOH solution at 60 ⁰C for 24 h) and filtered through a mill silk, 10 μm mesh size. Collected particles were categorized as: fibre, hard plastic, nylon, rubber, or miscellaneous. Categories were divided into subcategories based on the coloration of the particles. Particles ingested by organisms were represented mostly by fibres and fragmented hard plastics, within the size range were from 0.03 to
4.87 mm. A total of 678 MP particles were collected with an average of 4.64 ± 1.59; 1.64 ± 0.46 and 1.24 ± 0.34 items/organism isolated from L. hoffmeisteri, L. naticoides and C. acutiventris, respectively. According to results, L. hoffmeisteri, L. naticoides and C. acutiventris could be used as proper bioaccumulators of MP pollution in the Danube River.
PB  - Niš: University of Niš
T2  - Facta Universitatis. Series: Medicine and Biology
T1  - Isolation of microplastics from freshwater macroinvertebrates in the Danube River
IS  - 2
VL  - 23
DO  - 10.22190/FUMB211101006S
SP  - 21
EP  - 27
ER  - 

@article{
author = "Stanković, Jelena and Raković, Maja and Paunović, Momir and Atanacković, Ana and Tomović, Jelena and Milošević, Đurađ",
year = "2021",
abstract = "The study was conducted on the Danube River, within the project Joint Danube Survey 3 (JDS3). The main aim was to estimate the quantity of microplastics in aquatic ecosystems through passive biological monitoring. Three freshwater species were used for microplastic (MP) isolation from different taxonomic groups of organisms: Mollusca, Oligochaeta, and Chironomidae (Diptera), with the following species: Lithoglyphus naticoides (C. Pfeiffer, 1828), Limnodrilus hoffmeisteri (Claparede, 1862), and Chironomus acutiventris (Wülker, Ryser & Scholl, 1983), respectively. The samples were collected from 6 sites along the Danube River where 540 specimens were examined. The samples were digested by alkaline method (incubation in 10% KOH solution at 60 ⁰C for 24 h) and filtered through a mill silk, 10 μm mesh size. Collected particles were categorized as: fibre, hard plastic, nylon, rubber, or miscellaneous. Categories were divided into subcategories based on the coloration of the particles. Particles ingested by organisms were represented mostly by fibres and fragmented hard plastics, within the size range were from 0.03 to
4.87 mm. A total of 678 MP particles were collected with an average of 4.64 ± 1.59; 1.64 ± 0.46 and 1.24 ± 0.34 items/organism isolated from L. hoffmeisteri, L. naticoides and C. acutiventris, respectively. According to results, L. hoffmeisteri, L. naticoides and C. acutiventris could be used as proper bioaccumulators of MP pollution in the Danube River.",
publisher = "Niš: University of Niš",
journal = "Facta Universitatis. Series: Medicine and Biology",
title = "Isolation of microplastics from freshwater macroinvertebrates in the Danube River",
number = "2",
volume = "23",
doi = "10.22190/FUMB211101006S",
pages = "21-27"
}

Stanković, J., Raković, M., Paunović, M., Atanacković, A., Tomović, J.,& Milošević, Đ.. (2021). Isolation of microplastics from freshwater macroinvertebrates in the Danube River. in Facta Universitatis. Series: Medicine and Biology
Niš: University of Niš., 23(2), 21-27.
https://doi.org/10.22190/FUMB211101006S

Stanković J, Raković M, Paunović M, Atanacković A, Tomović J, Milošević Đ. Isolation of microplastics from freshwater macroinvertebrates in the Danube River. in Facta Universitatis. Series: Medicine and Biology. 2021;23(2):21-27.
doi:10.22190/FUMB211101006S .

Stanković, Jelena, Raković, Maja, Paunović, Momir, Atanacković, Ana, Tomović, Jelena, Milošević, Đurađ, "Isolation of microplastics from freshwater macroinvertebrates in the Danube River" in Facta Universitatis. Series: Medicine and Biology, 23, no. 2 (2021):21-27,
https://doi.org/10.22190/FUMB211101006S . .

TY  - JOUR
AU  - Novčić, Ivana
AU  - Krunić, Sara
AU  - Golubović, Ana
AU  - Zorić, Katarina
AU  - Haubner, Mark E.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4739
AB  - Data reliability is the degree to which the data can be replicated by the same or different observers and should be one of the most basic aspects of scientific data assessment. Yet, for example, information on the reliability of feeding rate data in shorebirds is strikingly lacking in the literature. In this study, the reliability of foraging behavior metrics collected on Semipalmated Sandpipers (Calidris pusilla) and Short-billed Dowitchers (Limnodromus griseus) during their spring stopovers in Delaware Bay, Maryland, USA, was examined. Using video recordings of 30 foraging individuals scored by four observers, including one experienced observer, assessed: (1) to what extent the data on these two species' foraging rates can be replicated while viewing videos at real-time speed as opposed to videos viewed in slow-motion; and (2) whether intra- and inter-observer reliabilities vary between species and with the observer experience. The results demonstrate that scoring feeding rates in Short-billed Dowitchers does not require the analysis of the footage in slow motion, implying that data may be obtained in real time in the field, following an appropriate training of observers. By contrast, detailed scoring of different foraging techniques in small sandpipers requires either slow-motion reviews of the field-sourced footage, or the clumping of foraging techniques into tactile and visual types to provide sufficient reliability of the data collected in the field if filming of birds is not feasible.
PB  - Washington: The Waterbird Society
T2  - Waterbirds
T1  - The reliability of measurements of foraging behavior in shorebirds: a comparison of real-time and slow-motion recordings
IS  - 1
VL  - 44
DO  - 10.1675/063.044.0106
SP  - 68
EP  - 75
ER  - 

@article{
author = "Novčić, Ivana and Krunić, Sara and Golubović, Ana and Zorić, Katarina and Haubner, Mark E.",
year = "2021",
abstract = "Data reliability is the degree to which the data can be replicated by the same or different observers and should be one of the most basic aspects of scientific data assessment. Yet, for example, information on the reliability of feeding rate data in shorebirds is strikingly lacking in the literature. In this study, the reliability of foraging behavior metrics collected on Semipalmated Sandpipers (Calidris pusilla) and Short-billed Dowitchers (Limnodromus griseus) during their spring stopovers in Delaware Bay, Maryland, USA, was examined. Using video recordings of 30 foraging individuals scored by four observers, including one experienced observer, assessed: (1) to what extent the data on these two species' foraging rates can be replicated while viewing videos at real-time speed as opposed to videos viewed in slow-motion; and (2) whether intra- and inter-observer reliabilities vary between species and with the observer experience. The results demonstrate that scoring feeding rates in Short-billed Dowitchers does not require the analysis of the footage in slow motion, implying that data may be obtained in real time in the field, following an appropriate training of observers. By contrast, detailed scoring of different foraging techniques in small sandpipers requires either slow-motion reviews of the field-sourced footage, or the clumping of foraging techniques into tactile and visual types to provide sufficient reliability of the data collected in the field if filming of birds is not feasible.",
publisher = "Washington: The Waterbird Society",
journal = "Waterbirds",
title = "The reliability of measurements of foraging behavior in shorebirds: a comparison of real-time and slow-motion recordings",
number = "1",
volume = "44",
doi = "10.1675/063.044.0106",
pages = "68-75"
}

Novčić, I., Krunić, S., Golubović, A., Zorić, K.,& Haubner, M. E.. (2021). The reliability of measurements of foraging behavior in shorebirds: a comparison of real-time and slow-motion recordings. in Waterbirds
Washington: The Waterbird Society., 44(1), 68-75.
https://doi.org/10.1675/063.044.0106

Novčić I, Krunić S, Golubović A, Zorić K, Haubner ME. The reliability of measurements of foraging behavior in shorebirds: a comparison of real-time and slow-motion recordings. in Waterbirds. 2021;44(1):68-75.
doi:10.1675/063.044.0106 .

Novčić, Ivana, Krunić, Sara, Golubović, Ana, Zorić, Katarina, Haubner, Mark E., "The reliability of measurements of foraging behavior in shorebirds: a comparison of real-time and slow-motion recordings" in Waterbirds, 44, no. 1 (2021):68-75,
https://doi.org/10.1675/063.044.0106 . .

TY  - CHAP
AU  - Raković, Maja
AU  - Paunović, Momir
AU  - Tomović, Jelena
AU  - Popović, Nataša
AU  - Csányi, Béla
AU  - Jovanović, Milica
AU  - Glöer, Peter
AU  - Pešić, Vladimir
PY  - 2020
UR  - http://link.springer.com/10.1007/698_2020_487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3650
AB  - Molluscs are diverse and abundant component of macroinvertebrate assemblages inhabiting different types of river ecosystems. According to the literature data, a total of 143 freshwater mollusc taxa have been recorded for freshwater ecosystems of Montenegro. Gastropoda was represented by 120 taxa from 14 families, while the Bivalvia was represented by 23 species from 4 families. The typology of river and lake water bodies of Montenegro has been carried out according to the EU Water Framework Directive (2000/60/EC). In this study we used a simple river typology of Montenegrin watercourses based on two descriptors, the river size and the altitude. Our preliminary result revealed that the obtained mollusc assemblage clusters satisfactorily describe two main river types, the lowland portions of the large rivers in the Adriatic Sea Basin and the large- and medium-sized rivers at the mid-altitude. For a more confident water body typology based on mollusc assemblages, additional studies are highly needed.
PB  - Springer
T2  - The Handbook of Environmental Chemistry
T2  - The Handbook of Environmental Chemistry
T1  - Do Molluscs Assemblages Reflect River Typology: A Case Study of Montenegro
DO  - 10.1007/698_2020_487
SP  - 265
EP  - 285
ER  - 

@inbook{
author = "Raković, Maja and Paunović, Momir and Tomović, Jelena and Popović, Nataša and Csányi, Béla and Jovanović, Milica and Glöer, Peter and Pešić, Vladimir",
year = "2020",
abstract = "Molluscs are diverse and abundant component of macroinvertebrate assemblages inhabiting different types of river ecosystems. According to the literature data, a total of 143 freshwater mollusc taxa have been recorded for freshwater ecosystems of Montenegro. Gastropoda was represented by 120 taxa from 14 families, while the Bivalvia was represented by 23 species from 4 families. The typology of river and lake water bodies of Montenegro has been carried out according to the EU Water Framework Directive (2000/60/EC). In this study we used a simple river typology of Montenegrin watercourses based on two descriptors, the river size and the altitude. Our preliminary result revealed that the obtained mollusc assemblage clusters satisfactorily describe two main river types, the lowland portions of the large rivers in the Adriatic Sea Basin and the large- and medium-sized rivers at the mid-altitude. For a more confident water body typology based on mollusc assemblages, additional studies are highly needed.",
publisher = "Springer",
journal = "The Handbook of Environmental Chemistry, The Handbook of Environmental Chemistry",
booktitle = "Do Molluscs Assemblages Reflect River Typology: A Case Study of Montenegro",
doi = "10.1007/698_2020_487",
pages = "265-285"
}

Raković, M., Paunović, M., Tomović, J., Popović, N., Csányi, B., Jovanović, M., Glöer, P.,& Pešić, V.. (2020). Do Molluscs Assemblages Reflect River Typology: A Case Study of Montenegro. in The Handbook of Environmental Chemistry
Springer., 265-285.
https://doi.org/10.1007/698_2020_487

Raković M, Paunović M, Tomović J, Popović N, Csányi B, Jovanović M, Glöer P, Pešić V. Do Molluscs Assemblages Reflect River Typology: A Case Study of Montenegro. in The Handbook of Environmental Chemistry. 2020;:265-285.
doi:10.1007/698_2020_487 .

Raković, Maja, Paunović, Momir, Tomović, Jelena, Popović, Nataša, Csányi, Béla, Jovanović, Milica, Glöer, Peter, Pešić, Vladimir, "Do Molluscs Assemblages Reflect River Typology: A Case Study of Montenegro" in The Handbook of Environmental Chemistry (2020):265-285,
https://doi.org/10.1007/698_2020_487 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Mitić Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Đukanović, Stefana
AU  - Marković, Tatjana
AU  - Tasić, Gvozden
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32707220
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3831
AB  - OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.
VL  - 117
DO  - 10.1016/j.archoralbio.2020.104842
SP  - 104842
ER  - 

@article{
author = "Marinković, Jelena and Mitić Ćulafić, Dragana and Nikolić, Biljana and Đukanović, Stefana and Marković, Tatjana and Tasić, Gvozden and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.",
volume = "117",
doi = "10.1016/j.archoralbio.2020.104842",
pages = "104842"
}

Marinković, J., Mitić Ćulafić, D., Nikolić, B., Đukanović, S., Marković, T., Tasić, G., Ćirić, A.,& Marković, D.. (2020). Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology
Elsevier Ltd., 117, 104842.
https://doi.org/10.1016/j.archoralbio.2020.104842

Marinković J, Mitić Ćulafić D, Nikolić B, Đukanović S, Marković T, Tasić G, Ćirić A, Marković D. Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology. 2020;117:104842.
doi:10.1016/j.archoralbio.2020.104842 .

Marinković, Jelena, Mitić Ćulafić, Dragana, Nikolić, Biljana, Đukanović, Stefana, Marković, Tatjana, Tasić, Gvozden, Ćirić, Ana, Marković, Dejan, "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals." in Archives of Oral Biology, 117 (2020):104842,
https://doi.org/10.1016/j.archoralbio.2020.104842 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Nikolić, Sonja Z.
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2020
UR  - https://www.mdpi.com/2076-2615/10/4/603
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3649
AB  - Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.
T2  - Animals
T1  - The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)
IS  - 4
VL  - 10
DO  - 10.3390/ani10040603
SP  - 603
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Nikolić, Sonja Z. and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Prokić, Marko",
year = "2020",
abstract = "Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.",
journal = "Animals",
title = "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)",
number = "4",
volume = "10",
doi = "10.3390/ani10040603",
pages = "603"
}

Petrović, T., Vučić, T., Nikolić, S. Z., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Prokić, M.. (2020). The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals, 10(4), 603.
https://doi.org/10.3390/ani10040603

Petrović T, Vučić T, Nikolić SZ, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Prokić M. The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals. 2020;10(4):603.
doi:10.3390/ani10040603 .

Petrović, Tamara, Vučić, Tijana, Nikolić, Sonja Z., Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Prokić, Marko, "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)" in Animals, 10, no. 4 (2020):603,
https://doi.org/10.3390/ani10040603 . .

TY  - JOUR
AU  - Đuknić, Jelena
AU  - Jovanović, Vladimir
AU  - Čanak Atlagić, Jelena
AU  - Anđus, Stefan
AU  - Paunović, Momir
AU  - Živić, Ivana
AU  - Popović, Nataša
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3644
AB  - Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 are two species of the Simulium reptans group whose distribution is unclear because of their confusing taxonomy and systematics. Their genetic variability is well known for populations in northern and central Europe and shows that both species have two forms; however, the genetic variability of these species in southern and eastern Europe is unknown. To identify the status of these two species in southeast Europe, mtDNA was extracted from 19 individuals from 12 localities across the Balkan Peninsula. Phylogenetic analysis confirmed the existence of two species with 7.38–7.94% divergence. Each species was comprised of two clades, with 2.31% and 1.43% interclade divergence for S. reptans and S. reptantoides , respectively. This study revealed the presence of both species across the Balkans and that S. reptans occurs in this area in only one form ( S. reptans B), while S. reptantoides is found in two genetic forms (A and B).
PB  - Pensoft Publishers
T2  - ZooKeys
T1  - Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula
VL  - 922
DO  - 10.3897/zookeys.922.49306
SP  - 141
EP  - 155
ER  - 

@article{
author = "Đuknić, Jelena and Jovanović, Vladimir and Čanak Atlagić, Jelena and Anđus, Stefan and Paunović, Momir and Živić, Ivana and Popović, Nataša",
year = "2020",
abstract = "Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 are two species of the Simulium reptans group whose distribution is unclear because of their confusing taxonomy and systematics. Their genetic variability is well known for populations in northern and central Europe and shows that both species have two forms; however, the genetic variability of these species in southern and eastern Europe is unknown. To identify the status of these two species in southeast Europe, mtDNA was extracted from 19 individuals from 12 localities across the Balkan Peninsula. Phylogenetic analysis confirmed the existence of two species with 7.38–7.94% divergence. Each species was comprised of two clades, with 2.31% and 1.43% interclade divergence for S. reptans and S. reptantoides , respectively. This study revealed the presence of both species across the Balkans and that S. reptans occurs in this area in only one form ( S. reptans B), while S. reptantoides is found in two genetic forms (A and B).",
publisher = "Pensoft Publishers",
journal = "ZooKeys",
title = "Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula",
volume = "922",
doi = "10.3897/zookeys.922.49306",
pages = "141-155"
}

Đuknić, J., Jovanović, V., Čanak Atlagić, J., Anđus, S., Paunović, M., Živić, I.,& Popović, N.. (2020). Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula. in ZooKeys
Pensoft Publishers., 922, 141-155.
https://doi.org/10.3897/zookeys.922.49306

Đuknić J, Jovanović V, Čanak Atlagić J, Anđus S, Paunović M, Živić I, Popović N. Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula. in ZooKeys. 2020;922:141-155.
doi:10.3897/zookeys.922.49306 .

Đuknić, Jelena, Jovanović, Vladimir, Čanak Atlagić, Jelena, Anđus, Stefan, Paunović, Momir, Živić, Ivana, Popović, Nataša, "Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula" in ZooKeys, 922 (2020):141-155,
https://doi.org/10.3897/zookeys.922.49306 . .