TY  - CONF
AU  - Jakubek, Patrycja
AU  - Rajić, Jovana
AU  - Baranowska, Monika
AU  - Vidaković, Melita
AU  - Namiesnik, Jacek
AU  - Bartoszek, Agnieszka
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6309
AB  - In our previous study, treatment of HT29 cell line with catechins induced dose-dependent changes in the expression of redox-related genes. Uniquely, only one gene (SRXN1, sulfiredoxin-1) was down-regulated upon treatment with 10 μM (-)-epigallocatechin (EGC). The aim of the current study was to investigate whether the observed down-regulation of SRXN1 expression was affected by epigenetic changes. HT29 cells were treated with catechins at different concentrations for 24 h and subsequently genomic DNA was isolated and bisulfite converted. DNA methylation profiles of selected regions within SRXN1 promoter were examined using Methylation-Specific PCR (MSP) and Methylation-Sensitive High Resolution Melting (MS-HRM). MSP analysis showed no differences in DNA methylation level between any of the treatments compared to control. However, the difference was observed when the bigger area of CpG island was analyzed by MS-HRM. Significant increase in DNA methylation level was observed after cell treatment with higher doses of EGC and (-)-epicatechin gallate (ECG). DNA demethylation requires oxidative modifications of methylated cytosine. Catechins, which are strong antioxidants, may lead to inhibition of DNA demethylation by changing cellular environment to more reduced state, especially in the case of higher doses. Thus, we report that catechins can act as methylation inducers and probably this function derives from their ability to influence cellular redox homeostasis.
PB  - Belgrade: NutRedox, COST Action CA16112
C3  - Nutraceuticals in balancing redox status in ageing and age-related diseases: Book of Abstracts. WGs Meeting of the NutRedOx COST Action CA16112; 2020 Mar 2-3; Belgrade, Serbia
T1  - The impact of catechins on DNA methylation level within promoter area of sulfiredoxin-1 gene in HT29 cell line
SP  - 25
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6309
ER  - 

@conference{
editor = "Dinić, Svetlana, Šunderić, Miloš, Vučić, Vesna, Vidović, Bojana",
author = "Jakubek, Patrycja and Rajić, Jovana and Baranowska, Monika and Vidaković, Melita and Namiesnik, Jacek and Bartoszek, Agnieszka",
year = "2020",
abstract = "In our previous study, treatment of HT29 cell line with catechins induced dose-dependent changes in the expression of redox-related genes. Uniquely, only one gene (SRXN1, sulfiredoxin-1) was down-regulated upon treatment with 10 μM (-)-epigallocatechin (EGC). The aim of the current study was to investigate whether the observed down-regulation of SRXN1 expression was affected by epigenetic changes. HT29 cells were treated with catechins at different concentrations for 24 h and subsequently genomic DNA was isolated and bisulfite converted. DNA methylation profiles of selected regions within SRXN1 promoter were examined using Methylation-Specific PCR (MSP) and Methylation-Sensitive High Resolution Melting (MS-HRM). MSP analysis showed no differences in DNA methylation level between any of the treatments compared to control. However, the difference was observed when the bigger area of CpG island was analyzed by MS-HRM. Significant increase in DNA methylation level was observed after cell treatment with higher doses of EGC and (-)-epicatechin gallate (ECG). DNA demethylation requires oxidative modifications of methylated cytosine. Catechins, which are strong antioxidants, may lead to inhibition of DNA demethylation by changing cellular environment to more reduced state, especially in the case of higher doses. Thus, we report that catechins can act as methylation inducers and probably this function derives from their ability to influence cellular redox homeostasis.",
publisher = "Belgrade: NutRedox, COST Action CA16112",
journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases: Book of Abstracts. WGs Meeting of the NutRedOx COST Action CA16112; 2020 Mar 2-3; Belgrade, Serbia",
title = "The impact of catechins on DNA methylation level within promoter area of sulfiredoxin-1 gene in HT29 cell line",
pages = "25-25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6309"
}

Dinić, S., Šunderić, M., Vučić, V., Vidović, B., Jakubek, P., Rajić, J., Baranowska, M., Vidaković, M., Namiesnik, J.,& Bartoszek, A.. (2020). The impact of catechins on DNA methylation level within promoter area of sulfiredoxin-1 gene in HT29 cell line. in Nutraceuticals in balancing redox status in ageing and age-related diseases: Book of Abstracts. WGs Meeting of the NutRedOx COST Action CA16112; 2020 Mar 2-3; Belgrade, Serbia
Belgrade: NutRedox, COST Action CA16112., 25-25.
https://hdl.handle.net/21.15107/rcub_ibiss_6309

Dinić S, Šunderić M, Vučić V, Vidović B, Jakubek P, Rajić J, Baranowska M, Vidaković M, Namiesnik J, Bartoszek A. The impact of catechins on DNA methylation level within promoter area of sulfiredoxin-1 gene in HT29 cell line. in Nutraceuticals in balancing redox status in ageing and age-related diseases: Book of Abstracts. WGs Meeting of the NutRedOx COST Action CA16112; 2020 Mar 2-3; Belgrade, Serbia. 2020;:25-25.
https://hdl.handle.net/21.15107/rcub_ibiss_6309 .

Dinić, Svetlana, Šunderić, Miloš, Vučić, Vesna, Vidović, Bojana, Jakubek, Patrycja, Rajić, Jovana, Baranowska, Monika, Vidaković, Melita, Namiesnik, Jacek, Bartoszek, Agnieszka, "The impact of catechins on DNA methylation level within promoter area of sulfiredoxin-1 gene in HT29 cell line" in Nutraceuticals in balancing redox status in ageing and age-related diseases: Book of Abstracts. WGs Meeting of the NutRedOx COST Action CA16112; 2020 Mar 2-3; Belgrade, Serbia (2020):25-25,
https://hdl.handle.net/21.15107/rcub_ibiss_6309 .

TY  - CONF
AU  - Jakubek, Patrycja
AU  - Baranowska, Monika
AU  - Rajić, Jovana
AU  - Vidaković, Melita
AU  - Namiesnik, Jacek
AU  - Bartoszek, Agnieszka
PY  - 2019
UR  - https://www.redox-medicine.com/images/2019/pdf/Paris_Redox_2019_-_Final_Agenda_-_V6.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6310
AB  - Introduction: Our previous research indicated that treatment of HT29 cells with different catechins affected the expression of redox-related genes in a dose dependent manner with only one gene (SRXN1) being down-regulated [1]. Since catechins were reported to affect DNA methylation levels [2], the objestive of current research was to find out wether the observed down-regulation of SRXN1 expression was caused by epigenetic changes.
Materials & Methods: Human colon adenocarcinoma HT29 cells were treated with 5 catechins at 4 concentrations for 24 hours and subsequently genomic DNA was isolated. To perform methylation analysis, DNA isolates were bisulfite converted using EZ DNA Methylation kit from Zymo Research (USA). DNA methylation profiles within the chosen fragment of promoter area of SRXN1 gene were examined using Methylation-Specific PCR and Methylation-Sensitive High Resolution Melting.
Results: According to Baranowska et al. [1], the treatment of HT29 cell line with 10 µM (-)-epigallocatechin caused down-regulation of SRXN1 gene. So, we hypothesized that the methylation level within the promoter CpG island of this gene will be increased by this compound and our presumption were confirmed. Besides, significant increase of DNA methylation was observed also for high dosed of (-)-epicatechin gallate.
Conclusion: Catechins may influence DNA methylation pattern of redox responsive genes.
PB  - Paris: University Pierre et Marie Curie
C3  - Abstracts book: 21st ISANH Interantional Conference on Oxidative stress, redox homeostasis and antioxidants; 2019 Jun 20-21; Paris, France
T1  - Modulation of DNA methylation profile of SRXN1 gene promoter in HT29 cells exposed to catechins of different redox activity
SP  - 42
EP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6310
ER  - 

@conference{
author = "Jakubek, Patrycja and Baranowska, Monika and Rajić, Jovana and Vidaković, Melita and Namiesnik, Jacek and Bartoszek, Agnieszka",
year = "2019",
abstract = "Introduction: Our previous research indicated that treatment of HT29 cells with different catechins affected the expression of redox-related genes in a dose dependent manner with only one gene (SRXN1) being down-regulated [1]. Since catechins were reported to affect DNA methylation levels [2], the objestive of current research was to find out wether the observed down-regulation of SRXN1 expression was caused by epigenetic changes.
Materials & Methods: Human colon adenocarcinoma HT29 cells were treated with 5 catechins at 4 concentrations for 24 hours and subsequently genomic DNA was isolated. To perform methylation analysis, DNA isolates were bisulfite converted using EZ DNA Methylation kit from Zymo Research (USA). DNA methylation profiles within the chosen fragment of promoter area of SRXN1 gene were examined using Methylation-Specific PCR and Methylation-Sensitive High Resolution Melting.
Results: According to Baranowska et al. [1], the treatment of HT29 cell line with 10 µM (-)-epigallocatechin caused down-regulation of SRXN1 gene. So, we hypothesized that the methylation level within the promoter CpG island of this gene will be increased by this compound and our presumption were confirmed. Besides, significant increase of DNA methylation was observed also for high dosed of (-)-epicatechin gallate.
Conclusion: Catechins may influence DNA methylation pattern of redox responsive genes.",
publisher = "Paris: University Pierre et Marie Curie",
journal = "Abstracts book: 21st ISANH Interantional Conference on Oxidative stress, redox homeostasis and antioxidants; 2019 Jun 20-21; Paris, France",
title = "Modulation of DNA methylation profile of SRXN1 gene promoter in HT29 cells exposed to catechins of different redox activity",
pages = "42-42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6310"
}

Jakubek, P., Baranowska, M., Rajić, J., Vidaković, M., Namiesnik, J.,& Bartoszek, A.. (2019). Modulation of DNA methylation profile of SRXN1 gene promoter in HT29 cells exposed to catechins of different redox activity. in Abstracts book: 21st ISANH Interantional Conference on Oxidative stress, redox homeostasis and antioxidants; 2019 Jun 20-21; Paris, France
Paris: University Pierre et Marie Curie., 42-42.
https://hdl.handle.net/21.15107/rcub_ibiss_6310

Jakubek P, Baranowska M, Rajić J, Vidaković M, Namiesnik J, Bartoszek A. Modulation of DNA methylation profile of SRXN1 gene promoter in HT29 cells exposed to catechins of different redox activity. in Abstracts book: 21st ISANH Interantional Conference on Oxidative stress, redox homeostasis and antioxidants; 2019 Jun 20-21; Paris, France. 2019;:42-42.
https://hdl.handle.net/21.15107/rcub_ibiss_6310 .

Jakubek, Patrycja, Baranowska, Monika, Rajić, Jovana, Vidaković, Melita, Namiesnik, Jacek, Bartoszek, Agnieszka, "Modulation of DNA methylation profile of SRXN1 gene promoter in HT29 cells exposed to catechins of different redox activity" in Abstracts book: 21st ISANH Interantional Conference on Oxidative stress, redox homeostasis and antioxidants; 2019 Jun 20-21; Paris, France (2019):42-42,
https://hdl.handle.net/21.15107/rcub_ibiss_6310 .

TY  - CONF
AU  - Jakubek, Patrycja
AU  - Baranowska, Monika
AU  - Rajić, Jovana
AU  - Vidaković, Melita
AU  - Bartoszek, Agnieszka
AU  - Namiesnik, Jacek
PY  - 2018
UR  - https://karger.com/lfg/article/11/1/19/188281/3rd-European-Summer-School-on-Nutrigenomics
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6311
AB  - Objectives: Year by year, a growing public interest in relationship between diet and ageing can be observed. In particular, this refers to antioxidants, a group of compounds present in food and beverages that are expected to have beneficial health effects. This group includes catechins (favan-3-ols), which are known to play a role in modulation of cellular redox homeostasis and various signaling pathways.
Our previous results have indicated that in HT29 cell line, changes in expression of some genes associated with oxidative stress response are dependent on concentration of catechins. In the subsequent experiments,, we aim at examining the methylation profiles in the promoter areas of up-regulated and down-regulated genes. The objective of the research is to find out whether the observed dose dependence is caused by epigenetic modulation of gene expression.
Methods: The investigation of the impact of different catechins on the expression of genes involved in redox homeostasis was conducted using Oxidative Stress and Antioxidant Defense PCR Array from Qiagen, while DNA methylation is examined using Methylation-Specific PCR (MS-PCR) and Methylation-Sensitive High Resolution Melting (MS-HRM) methods.
Results: A significant up-regulation of 3 genes (ALB, CCL5, HSPA1A) out of 84 included into antioxidant response gene array was observed after treatment of HT29 cell line with investigated catechins at 1 µM concentration. No such up-regulation was seen at 10 µM catechins. Moreover, at this higher dose (-)-epigallocatechin caused down-regulation of SRXN1 gene within the examined gene panel.
Conclusion: Our methylation analysis included two antioxidant response genes, SRXN1 and HSPA1A, whose expression due to its promoter methylation is already documented. Our analysis of the SRXN1 gene regulatory region revealed presence of 706 bp long CpG island that encompasses promotor region and transcription start site, hence conducive to epigenetic silencing via DNA methylation. The impact of DNA methylation on gene expression in control and catechin-treated HT29 cells is in progress.
PB  - Karger Publishers:
C3  - Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy
T1  - Catechins as Potential Epigenetic Modulators
DO  - 10.1159/000490753
SP  - 8
EP  - 8
ER  - 

@conference{
author = "Jakubek, Patrycja and Baranowska, Monika and Rajić, Jovana and Vidaković, Melita and Bartoszek, Agnieszka and Namiesnik, Jacek",
year = "2018",
abstract = "Objectives: Year by year, a growing public interest in relationship between diet and ageing can be observed. In particular, this refers to antioxidants, a group of compounds present in food and beverages that are expected to have beneficial health effects. This group includes catechins (favan-3-ols), which are known to play a role in modulation of cellular redox homeostasis and various signaling pathways.
Our previous results have indicated that in HT29 cell line, changes in expression of some genes associated with oxidative stress response are dependent on concentration of catechins. In the subsequent experiments,, we aim at examining the methylation profiles in the promoter areas of up-regulated and down-regulated genes. The objective of the research is to find out whether the observed dose dependence is caused by epigenetic modulation of gene expression.
Methods: The investigation of the impact of different catechins on the expression of genes involved in redox homeostasis was conducted using Oxidative Stress and Antioxidant Defense PCR Array from Qiagen, while DNA methylation is examined using Methylation-Specific PCR (MS-PCR) and Methylation-Sensitive High Resolution Melting (MS-HRM) methods.
Results: A significant up-regulation of 3 genes (ALB, CCL5, HSPA1A) out of 84 included into antioxidant response gene array was observed after treatment of HT29 cell line with investigated catechins at 1 µM concentration. No such up-regulation was seen at 10 µM catechins. Moreover, at this higher dose (-)-epigallocatechin caused down-regulation of SRXN1 gene within the examined gene panel.
Conclusion: Our methylation analysis included two antioxidant response genes, SRXN1 and HSPA1A, whose expression due to its promoter methylation is already documented. Our analysis of the SRXN1 gene regulatory region revealed presence of 706 bp long CpG island that encompasses promotor region and transcription start site, hence conducive to epigenetic silencing via DNA methylation. The impact of DNA methylation on gene expression in control and catechin-treated HT29 cells is in progress.",
publisher = "Karger Publishers:",
journal = "Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy",
title = "Catechins as Potential Epigenetic Modulators",
doi = "10.1159/000490753",
pages = "8-8"
}

Jakubek, P., Baranowska, M., Rajić, J., Vidaković, M., Bartoszek, A.,& Namiesnik, J.. (2018). Catechins as Potential Epigenetic Modulators. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy
Karger Publishers:., 8-8.
https://doi.org/10.1159/000490753

Jakubek P, Baranowska M, Rajić J, Vidaković M, Bartoszek A, Namiesnik J. Catechins as Potential Epigenetic Modulators. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy. 2018;:8-8.
doi:10.1159/000490753 .

Jakubek, Patrycja, Baranowska, Monika, Rajić, Jovana, Vidaković, Melita, Bartoszek, Agnieszka, Namiesnik, Jacek, "Catechins as Potential Epigenetic Modulators" in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy (2018):8-8,
https://doi.org/10.1159/000490753 . .