TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Zoran Lj.
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5268
AB  - The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5268
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Petrović, Zoran Lj. and Škoro, Nikola and Puač, Nevena",
year = "2022",
abstract = "The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5268"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Petrović, Z. Lj., Škoro, N.,& Puač, N.. (2022). Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268

Živković S, Jevremović S, Gašić U, Milutinović M, Petrović ZL, Škoro N, Puač N. Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Petrović, Zoran Lj., Škoro, Nikola, Puač, Nevena, "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):137,
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Devrnja, Nina
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Puač, Nevena
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5269
AB  - Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Molecular response to PAW in model plant species
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5269
ER  - 

@conference{
author = "Milutinović, Milica and Jovanović, Olivera and Devrnja, Nina and Todorović, Miloš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Puač, Nevena and Škoro, Nikola",
year = "2022",
abstract = "Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Molecular response to PAW in model plant species",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5269"
}

Milutinović, M., Jovanović, O., Devrnja, N., Todorović, M., Živković, S., Savić, J., Skorić, M., Puač, N.,& Škoro, N.. (2022). Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269

Milutinović M, Jovanović O, Devrnja N, Todorović M, Živković S, Savić J, Skorić M, Puač N, Škoro N. Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

Milutinović, Milica, Jovanović, Olivera, Devrnja, Nina, Todorović, Miloš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Puač, Nevena, Škoro, Nikola, "Molecular response to PAW in model plant species" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .