TY  - JOUR
AU  - Banković, Jasna Z.
AU  - Joerg, Andrae
AU  - Todorović, Nataša
AU  - Podolski-Renić, Ana
AU  - Milošević, Zorica Z.
AU  - Miljković, Đorđe
AU  - Krause, Jannike
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola
AU  - Pešić, Milica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1018
AB  - Most chemotherapeutics harm normal cells causing severe side effects and induce the development of resistance in cancer cells. Antimicrobial peptides (AMPs), recognized as anticancer agents, may overcome these limitations. The most studied mechanism underlying multidrug resistance (MDR) is the over-expression of cell membrane transporter P-glycoprotein (P-gp), which extrudes a variety of hydrophobic drugs. Additionally, P-gp contributes to cell membrane composition and increases the net negative charge on cell surface. We postulated that NK-lysin derived cationic peptide NK-2 might discriminate and preferentially eliminate P-gp over-expressing cancer cells. To test this hypothesis, we employed MDR non-small cell lung carcinoma (NCI-H460/R) and colorectal carcinoma (DLD1-TxR) cell lines with high P-gp expression. MDR cancer cells that survived NK-2 treatment had decreased P-gp expression and were more susceptible to doxorubicin. We found that NK-2 more readily eliminated P-gp high-expressing cells. Acting in 'carpet-like' manner NK-2 co-localized with P-gp on the MDR cancer cell membrane. The inhibition of P-gp reduced the NK-2 effect in MDR cancer cells and, vice versa, NK-2 decreased P-gp transport activity. In conclusion, NK-2 could modulate MDR in unique way, eliminating the P-gp high-expressing cells from heterogeneous cancers and making them more vulnerable to classical drug treatment. (C) 2012 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Experimental Cell Research
T1  - The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation
IS  - 7
VL  - 319
DO  - 10.1016/j.yexcr.2012.12.017
SP  - 1013
EP  - 1027
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1018
ER  - 

@article{
author = "Banković, Jasna Z. and Joerg, Andrae and Todorović, Nataša and Podolski-Renić, Ana and Milošević, Zorica Z. and Miljković, Đorđe and Krause, Jannike and Ruždijić, Sabera and Tanić, Nikola and Pešić, Milica",
year = "2013",
abstract = "Most chemotherapeutics harm normal cells causing severe side effects and induce the development of resistance in cancer cells. Antimicrobial peptides (AMPs), recognized as anticancer agents, may overcome these limitations. The most studied mechanism underlying multidrug resistance (MDR) is the over-expression of cell membrane transporter P-glycoprotein (P-gp), which extrudes a variety of hydrophobic drugs. Additionally, P-gp contributes to cell membrane composition and increases the net negative charge on cell surface. We postulated that NK-lysin derived cationic peptide NK-2 might discriminate and preferentially eliminate P-gp over-expressing cancer cells. To test this hypothesis, we employed MDR non-small cell lung carcinoma (NCI-H460/R) and colorectal carcinoma (DLD1-TxR) cell lines with high P-gp expression. MDR cancer cells that survived NK-2 treatment had decreased P-gp expression and were more susceptible to doxorubicin. We found that NK-2 more readily eliminated P-gp high-expressing cells. Acting in 'carpet-like' manner NK-2 co-localized with P-gp on the MDR cancer cell membrane. The inhibition of P-gp reduced the NK-2 effect in MDR cancer cells and, vice versa, NK-2 decreased P-gp transport activity. In conclusion, NK-2 could modulate MDR in unique way, eliminating the P-gp high-expressing cells from heterogeneous cancers and making them more vulnerable to classical drug treatment. (C) 2012 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Experimental Cell Research",
title = "The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation",
number = "7",
volume = "319",
doi = "10.1016/j.yexcr.2012.12.017",
pages = "1013-1027",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1018"
}

Banković, J. Z., Joerg, A., Todorović, N., Podolski-Renić, A., Milošević, Z. Z., Miljković, Đ., Krause, J., Ruždijić, S., Tanić, N.,& Pešić, M.. (2013). The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation. in Experimental Cell Research
Elsevier., 319(7), 1013-1027.
https://doi.org/10.1016/j.yexcr.2012.12.017
https://hdl.handle.net/21.15107/rcub_ibiss_1018

Banković JZ, Joerg A, Todorović N, Podolski-Renić A, Milošević ZZ, Miljković Đ, Krause J, Ruždijić S, Tanić N, Pešić M. The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation. in Experimental Cell Research. 2013;319(7):1013-1027.
doi:10.1016/j.yexcr.2012.12.017
https://hdl.handle.net/21.15107/rcub_ibiss_1018 .

Banković, Jasna Z., Joerg, Andrae, Todorović, Nataša, Podolski-Renić, Ana, Milošević, Zorica Z., Miljković, Đorđe, Krause, Jannike, Ruždijić, Sabera, Tanić, Nikola, Pešić, Milica, "The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation" in Experimental Cell Research, 319, no. 7 (2013):1013-1027,
https://doi.org/10.1016/j.yexcr.2012.12.017 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1018 .