TY  - CONF
AU  - Mihailović, Mirjana
AU  - Petrović, Miodrag
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Ivanović-Matić, Svetlana
AU  - Martinović, Vesna
AU  - Arambašić Jovanović, Jelena
AU  - Poznanović, Goran
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5653
AB  - Metallothioneins (MTs) constitute a family of low-molecular  weight, cysteine­ rich and heat stable 
proteins involved in the binding and regulation of essential metals, such as copper and zinc, and 
in the detoxification of these and other non-essential metals, such as cadmium and mercury. 
Previously we showed that the induction of MTs in Merluccius merluccius and Mui/us barbatus 
correlated with elevated concentrations of Cu and Pb, determined by chemical analysis of the 
seawater from Bar and Valdanos. In the present study we studied protein expression of MTs in the 
hepatopancreas of several seawater fish species: Red mullet (Mui/us barbatus), European hake 
(Merluccius merluccius), Tub gurnard (Trig/a lucerna) and Thinlip mullet  (Liza ramada), from the 
Monetengrin Adriatic coastline. Western blot analysis revealed the highest induction of MTs in the 
hepatopancreas in the following order: Merluccius merluccius, Trig/a lucerna, Mui/us barbatus and 
Liza ramada. Considering that MTs play a role in the metabolism of essential metals, they  are 
constitutively expressed. The presence of elevated concentrations of both essential and toxic 
metals provokes the induction of MTs. These results are in correlation with literature data showing 
that fish species differ in their detoxification capacities and amounts of accumulated  metals.  We 
 conclude that the level of induction of MTs in the hepatopancreas of the examined fish species 
correlates with the level of their reliability as bioindicator species in heavy metal 
biomonitoring.
PB  - Kotor, Montenegro : University of Montenegro, Institute of Marine Biology
C3  - Book of Abstracts: 1st International scientific conference integrated coastal zone management in the Adriatic sea; 2014 Sep 29 - Oct 1; Kotor, Montenegro
T1  - Species-specific expression of metallothionein in the hepatopancreas of seawater fish from the Montenegrin Adriatic coastline
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5653
ER  - 

@conference{
author = "Mihailović, Mirjana and Petrović, Miodrag and Grdović, Nevena and Dinić, Svetlana and Uskoković, Aleksandra and Vidaković, Melita and Grigorov, Ilijana and Bogojević, Desanka and Ivanović-Matić, Svetlana and Martinović, Vesna and Arambašić Jovanović, Jelena and Poznanović, Goran",
year = "2014",
abstract = "Metallothioneins (MTs) constitute a family of low-molecular  weight, cysteine­ rich and heat stable 
proteins involved in the binding and regulation of essential metals, such as copper and zinc, and 
in the detoxification of these and other non-essential metals, such as cadmium and mercury. 
Previously we showed that the induction of MTs in Merluccius merluccius and Mui/us barbatus 
correlated with elevated concentrations of Cu and Pb, determined by chemical analysis of the 
seawater from Bar and Valdanos. In the present study we studied protein expression of MTs in the 
hepatopancreas of several seawater fish species: Red mullet (Mui/us barbatus), European hake 
(Merluccius merluccius), Tub gurnard (Trig/a lucerna) and Thinlip mullet  (Liza ramada), from the 
Monetengrin Adriatic coastline. Western blot analysis revealed the highest induction of MTs in the 
hepatopancreas in the following order: Merluccius merluccius, Trig/a lucerna, Mui/us barbatus and 
Liza ramada. Considering that MTs play a role in the metabolism of essential metals, they  are 
constitutively expressed. The presence of elevated concentrations of both essential and toxic 
metals provokes the induction of MTs. These results are in correlation with literature data showing 
that fish species differ in their detoxification capacities and amounts of accumulated  metals.  We 
 conclude that the level of induction of MTs in the hepatopancreas of the examined fish species 
correlates with the level of their reliability as bioindicator species in heavy metal 
biomonitoring.",
publisher = "Kotor, Montenegro : University of Montenegro, Institute of Marine Biology",
journal = "Book of Abstracts: 1st International scientific conference integrated coastal zone management in the Adriatic sea; 2014 Sep 29 - Oct 1; Kotor, Montenegro",
title = "Species-specific expression of metallothionein in the hepatopancreas of seawater fish from the Montenegrin Adriatic coastline",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5653"
}

Mihailović, M., Petrović, M., Grdović, N., Dinić, S., Uskoković, A., Vidaković, M., Grigorov, I., Bogojević, D., Ivanović-Matić, S., Martinović, V., Arambašić Jovanović, J.,& Poznanović, G.. (2014). Species-specific expression of metallothionein in the hepatopancreas of seawater fish from the Montenegrin Adriatic coastline. in Book of Abstracts: 1st International scientific conference integrated coastal zone management in the Adriatic sea; 2014 Sep 29 - Oct 1; Kotor, Montenegro
Kotor, Montenegro : University of Montenegro, Institute of Marine Biology., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_5653

Mihailović M, Petrović M, Grdović N, Dinić S, Uskoković A, Vidaković M, Grigorov I, Bogojević D, Ivanović-Matić S, Martinović V, Arambašić Jovanović J, Poznanović G. Species-specific expression of metallothionein in the hepatopancreas of seawater fish from the Montenegrin Adriatic coastline. in Book of Abstracts: 1st International scientific conference integrated coastal zone management in the Adriatic sea; 2014 Sep 29 - Oct 1; Kotor, Montenegro. 2014;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_5653 .

Mihailović, Mirjana, Petrović, Miodrag, Grdović, Nevena, Dinić, Svetlana, Uskoković, Aleksandra, Vidaković, Melita, Grigorov, Ilijana, Bogojević, Desanka, Ivanović-Matić, Svetlana, Martinović, Vesna, Arambašić Jovanović, Jelena, Poznanović, Goran, "Species-specific expression of metallothionein in the hepatopancreas of seawater fish from the Montenegrin Adriatic coastline" in Book of Abstracts: 1st International scientific conference integrated coastal zone management in the Adriatic sea; 2014 Sep 29 - Oct 1; Kotor, Montenegro (2014):28,
https://hdl.handle.net/21.15107/rcub_ibiss_5653 .

TY  - CONF
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Bogojević, Desanka
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Grigorov, Ilijana
AU  - Ivanović-Matić, Svetlana
AU  - Martinović, Vesna
AU  - Petrović, Miodrag
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Poznanović, Goran
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5656
AB  - In order to ,issess effects of environme ntal pollutants on aqu at ic ecosystem s, different biomarkers may be exa mined.  The  pha se  I  biotransforma t ion e nzymes, notab ly CYP l A, definitely belong to t he most sensitive fish biomarker s known at present. CYP l A is a well-established biomarker  of  exposure  of  fish  to  xenobiotic  compounds.  The  CY P l A  protein  le vels can  be  determined  immunologicaly  ,  using   mono-   or polyclonal  antibodies  with  ELISA,  Western-blotting   or   histochemic  al technique  s.  Generally,  a  good  correlation  is  observed  between  CYP I A mRNA , protein le ve l s and CYP I A activity.  ln  this  work  we  per fo rm ed Weste r-b lot analys is and our aim was to characterize the  in du c ti on  of  CYPIA in he patopanc reas of the  European  hake-Merluccius mer /ucc ius and Red mul let-Mu l/us barbatus and in  winter  and  spring  at  the  mouth  of  th e river  Bojana.  Both  species  are  of  considerable  commercial  import ance Whereas intersp ecies differences in protein profiles were established, no qualitative  or  quantitative  intraspecies  or  seasonal  variations  in  protein  profiles were observed. Western blot analysis with a polyclonal antibody to  CYP1A reve aled the CYP I A expression in both  examined  fish  species  in winter  at  the  very  low  level.   However,   the   relative   concent!"ations   of CYP I A were increased in sprin g. The apparent initiation of  the  defens e response in fish  suggests  that  the  contamin  ants  present  in  seawater  have  an eco toxico logic al potentia l. These findings could be interpreted as an ea rly­ warning  signal for the deleter ious effects  of  the  pollutants.  The  higher  le vel of CYP l A in spr in g could  be connecte d with increased antopo genic ac ti v ity at the mouth  of  the  river Bojana  in  this  part  of the year.
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5656
ER  - 

@conference{
author = "Mihailović, Mirjana and Arambašić Jovanović, Jelena and Bogojević, Desanka and Dinić, Svetlana and Grdović, Nevena and Grigorov, Ilijana and Ivanović-Matić, Svetlana and Martinović, Vesna and Petrović, Miodrag and Uskoković, Aleksandra and Vidaković, Melita and Poznanović, Goran",
year = "2007",
abstract = "In order to ,issess effects of environme ntal pollutants on aqu at ic ecosystem s, different biomarkers may be exa mined.  The  pha se  I  biotransforma t ion e nzymes, notab ly CYP l A, definitely belong to t he most sensitive fish biomarker s known at present. CYP l A is a well-established biomarker  of  exposure  of  fish  to  xenobiotic  compounds.  The  CY P l A  protein  le vels can  be  determined  immunologicaly  ,  using   mono-   or polyclonal  antibodies  with  ELISA,  Western-blotting   or   histochemic  al technique  s.  Generally,  a  good  correlation  is  observed  between  CYP I A mRNA , protein le ve l s and CYP I A activity.  ln  this  work  we  per fo rm ed Weste r-b lot analys is and our aim was to characterize the  in du c ti on  of  CYPIA in he patopanc reas of the  European  hake-Merluccius mer /ucc ius and Red mul let-Mu l/us barbatus and in  winter  and  spring  at  the  mouth  of  th e river  Bojana.  Both  species  are  of  considerable  commercial  import ance Whereas intersp ecies differences in protein profiles were established, no qualitative  or  quantitative  intraspecies  or  seasonal  variations  in  protein  profiles were observed. Western blot analysis with a polyclonal antibody to  CYP1A reve aled the CYP I A expression in both  examined  fish  species  in winter  at  the  very  low  level.   However,   the   relative   concent!"ations   of CYP I A were increased in sprin g. The apparent initiation of  the  defens e response in fish  suggests  that  the  contamin  ants  present  in  seawater  have  an eco toxico logic al potentia l. These findings could be interpreted as an ea rly­ warning  signal for the deleter ious effects  of  the  pollutants.  The  higher  le vel of CYP l A in spr in g could  be connecte d with increased antopo genic ac ti v ity at the mouth  of  the  river Bojana  in  this  part  of the year.",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5656"
}

Mihailović, M., Arambašić Jovanović, J., Bogojević, D., Dinić, S., Grdović, N., Grigorov, I., Ivanović-Matić, S., Martinović, V., Petrović, M., Uskoković, A., Vidaković, M.,& Poznanović, G.. (2007). Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology..
https://hdl.handle.net/21.15107/rcub_ibiss_5656

Mihailović M, Arambašić Jovanović J, Bogojević D, Dinić S, Grdović N, Grigorov I, Ivanović-Matić S, Martinović V, Petrović M, Uskoković A, Vidaković M, Poznanović G. Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;.
https://hdl.handle.net/21.15107/rcub_ibiss_5656 .

Mihailović, Mirjana, Arambašić Jovanović, Jelena, Bogojević, Desanka, Dinić, Svetlana, Grdović, Nevena, Grigorov, Ilijana, Ivanović-Matić, Svetlana, Martinović, Vesna, Petrović, Miodrag, Uskoković, Aleksandra, Vidaković, Melita, Poznanović, Goran, "Seasonal depending expression of CYP1A in the hepatopancreas of Merluccius merluccius and Mullus barbatus at the mouth of the river Bojana" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_5656 .