TY  - JOUR
AU  - Tatalović, Nikola
AU  - Vidonja Uzelac, Teodora
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4662
AB  - Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS
production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since
the role of KATP channels and β -adrenoceptors in ROS cellular circuit was established here we
explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e.,
28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca2+-stimulated) and contractility
and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and
frequency of spontaneous active uteri immediately after addition that was prevented by propranolol
( 1 and  2 adrenoceptors selective antagonists) and glibenclamide (KATP sensitive channels inhibitor;
only frequency) pre-treatment. In Ca2+-stimulated uteri, ibogaine decreased both amplitude and
frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering,
while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in
SOD1 and an increase in CAT activity after 2 h. In Ca2+-stimulated uterus, there was also a decrease
of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity
increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of
antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β -adrenergic
receptors and KATP channels mediated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111792
SP  - 1792
ER  - 

@article{
author = "Tatalović, Nikola and Vidonja Uzelac, Teodora and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Bresjanac, Mara and Blagojević, Duško",
year = "2021",
abstract = "Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS
production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since
the role of KATP channels and β -adrenoceptors in ROS cellular circuit was established here we
explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e.,
28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca2+-stimulated) and contractility
and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and
frequency of spontaneous active uteri immediately after addition that was prevented by propranolol
( 1 and  2 adrenoceptors selective antagonists) and glibenclamide (KATP sensitive channels inhibitor;
only frequency) pre-treatment. In Ca2+-stimulated uteri, ibogaine decreased both amplitude and
frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering,
while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in
SOD1 and an increase in CAT activity after 2 h. In Ca2+-stimulated uterus, there was also a decrease
of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity
increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of
antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β -adrenergic
receptors and KATP channels mediated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated",
number = "11",
volume = "10",
doi = "10.3390/antiox10111792",
pages = "1792"
}

Tatalović, N., Vidonja Uzelac, T., Oreščanin Dušić, Z., Nikolić-Kokić, A., Bresjanac, M.,& Blagojević, D.. (2021). Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. in Antioxidants
Basel: MDPI., 10(11), 1792.
https://doi.org/10.3390/antiox10111792

Tatalović N, Vidonja Uzelac T, Oreščanin Dušić Z, Nikolić-Kokić A, Bresjanac M, Blagojević D. Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated. in Antioxidants. 2021;10(11):1792.
doi:10.3390/antiox10111792 .

Tatalović, Nikola, Vidonja Uzelac, Teodora, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Bresjanac, Mara, Blagojević, Duško, "Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated" in Antioxidants, 10, no. 11 (2021):1792,
https://doi.org/10.3390/antiox10111792 . .