TY  - CONF
AU  - Dožić, Aleksandra
AU  - Ćetković, Dejan
AU  - Despotović, Ana
AU  - Janjetović, Kristina
AU  - Zogović, Nevena
AU  - Antonijević, Đorđe
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5739
AB  - The aim of this study was to investigate the properties of experimental calcium aluminate (CA) dental cement, synthesized from CaO×Al2O3 and CaCO3. Calcium silicate (Portland cement, PC) served as a control. The elastic modulus and maximum stress obtained using the universal testing machine showed that CA has greater mechanical resistance than the control PC. Scanning electron microscopy (SEM) analysis of cements specimens before and after soaking in phosphate buffer saline showed that hydrated cements exposed crystal particles of calcite and new aluminum containing phases on their surfaces, suggesting their bioactive potential. Biocompatibility of the CA dental cement was evaluated by observing L929 cells morphology using phase-contrast microscopy. Cells treated with CA extract preserved their structural integrity without any changes in cell morphology, but with a slightly inhibited proliferation rate after 24h treatment, while PC induced changes typical for cell death.
PB  - Belgrade: Society of Physical Chemists of Serbia
C3  - Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia
T1  - Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement
SP  - 327
EP  - 330
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5739
ER  - 

@conference{
author = "Dožić, Aleksandra and Ćetković, Dejan and Despotović, Ana and Janjetović, Kristina and Zogović, Nevena and Antonijević, Đorđe",
year = "2022",
abstract = "The aim of this study was to investigate the properties of experimental calcium aluminate (CA) dental cement, synthesized from CaO×Al2O3 and CaCO3. Calcium silicate (Portland cement, PC) served as a control. The elastic modulus and maximum stress obtained using the universal testing machine showed that CA has greater mechanical resistance than the control PC. Scanning electron microscopy (SEM) analysis of cements specimens before and after soaking in phosphate buffer saline showed that hydrated cements exposed crystal particles of calcite and new aluminum containing phases on their surfaces, suggesting their bioactive potential. Biocompatibility of the CA dental cement was evaluated by observing L929 cells morphology using phase-contrast microscopy. Cells treated with CA extract preserved their structural integrity without any changes in cell morphology, but with a slightly inhibited proliferation rate after 24h treatment, while PC induced changes typical for cell death.",
publisher = "Belgrade: Society of Physical Chemists of Serbia",
journal = "Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia",
title = "Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement",
pages = "327-330",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5739"
}

Dožić, A., Ćetković, D., Despotović, A., Janjetović, K., Zogović, N.,& Antonijević, Đ.. (2022). Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia
Belgrade: Society of Physical Chemists of Serbia., 327-330.
https://hdl.handle.net/21.15107/rcub_ibiss_5739

Dožić A, Ćetković D, Despotović A, Janjetović K, Zogović N, Antonijević Đ. Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia. 2022;:327-330.
https://hdl.handle.net/21.15107/rcub_ibiss_5739 .

Dožić, Aleksandra, Ćetković, Dejan, Despotović, Ana, Janjetović, Kristina, Zogović, Nevena, Antonijević, Đorđe, "Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement" in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia (2022):327-330,
https://hdl.handle.net/21.15107/rcub_ibiss_5739 .

TY  - CONF
AU  - Ćetković, Dejan
AU  - Dožić, Aleksandra
AU  - Despotović, Ana
AU  - Janjetović, Kristina
AU  - Zogović, Nevena
AU  - Antonijević, Đorđe
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5738
AB  - This study aimed to investigate the influence of ZrO2, Bi2O3 and SrF2 added as radiopacifying agents (30wt.%) into calcium silicate/hydroxyapatite-based dental cement on its physical and biological properties. Among investigated cements, the mixture containing Bi2O3 had the highest values of elastic modules and toughness, similarly to control – mineral trioxide aggregate (MTA). SEM analysis of all hydrated cements has shown that bioactive calcite and tobermorite phases were formed. Crystal violet assay showed that pure (undiluted) extracts of experimental cements did not affect cell viability, while MTA exhibited an extremely cytotoxic effect on L929 cells. In 1:4 dilutions all experimental mixtures significantly increased cell proliferation potential after 72h in comparison to untreated cells and MTA, which cytotoxic effect diminished with dilutions. Further studies are needed to determine which radiopacifyer has the most desirable properties for adequate dental cement fabrication.
PB  - Belgrade: Society of Physical Chemists of Serbia
C3  - Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia
T1  - Effect of various radiopacifiers on selected physical properties and cytotoxicity of calcium silicate based dental cement enriched with hydroxyapatite
SP  - 323
EP  - 326
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5738
ER  - 

@conference{
author = "Ćetković, Dejan and Dožić, Aleksandra and Despotović, Ana and Janjetović, Kristina and Zogović, Nevena and Antonijević, Đorđe",
year = "2022",
abstract = "This study aimed to investigate the influence of ZrO2, Bi2O3 and SrF2 added as radiopacifying agents (30wt.%) into calcium silicate/hydroxyapatite-based dental cement on its physical and biological properties. Among investigated cements, the mixture containing Bi2O3 had the highest values of elastic modules and toughness, similarly to control – mineral trioxide aggregate (MTA). SEM analysis of all hydrated cements has shown that bioactive calcite and tobermorite phases were formed. Crystal violet assay showed that pure (undiluted) extracts of experimental cements did not affect cell viability, while MTA exhibited an extremely cytotoxic effect on L929 cells. In 1:4 dilutions all experimental mixtures significantly increased cell proliferation potential after 72h in comparison to untreated cells and MTA, which cytotoxic effect diminished with dilutions. Further studies are needed to determine which radiopacifyer has the most desirable properties for adequate dental cement fabrication.",
publisher = "Belgrade: Society of Physical Chemists of Serbia",
journal = "Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia",
title = "Effect of various radiopacifiers on selected physical properties and cytotoxicity of calcium silicate based dental cement enriched with hydroxyapatite",
pages = "323-326",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5738"
}

Ćetković, D., Dožić, A., Despotović, A., Janjetović, K., Zogović, N.,& Antonijević, Đ.. (2022). Effect of various radiopacifiers on selected physical properties and cytotoxicity of calcium silicate based dental cement enriched with hydroxyapatite. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia
Belgrade: Society of Physical Chemists of Serbia., 323-326.
https://hdl.handle.net/21.15107/rcub_ibiss_5738

Ćetković D, Dožić A, Despotović A, Janjetović K, Zogović N, Antonijević Đ. Effect of various radiopacifiers on selected physical properties and cytotoxicity of calcium silicate based dental cement enriched with hydroxyapatite. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia. 2022;:323-326.
https://hdl.handle.net/21.15107/rcub_ibiss_5738 .

Ćetković, Dejan, Dožić, Aleksandra, Despotović, Ana, Janjetović, Kristina, Zogović, Nevena, Antonijević, Đorđe, "Effect of various radiopacifiers on selected physical properties and cytotoxicity of calcium silicate based dental cement enriched with hydroxyapatite" in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 2; 2022 Sep 26-30; Belgrade, Serbia (2022):323-326,
https://hdl.handle.net/21.15107/rcub_ibiss_5738 .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biočanin, Vladimir
AU  - Milošević, Miloš
AU  - Trišić, Dijana
AU  - Lazović, Vladimir
AU  - Zogović, Nevena
AU  - Milašin, Jelena
AU  - Ilić, Dragan
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0272884221020794
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4464
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
PB  - Oxford : Elsevier
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
IS  - 20
VL  - 47
DO  - 10.1016/j.ceramint.2021.07.052
SP  - 28913
EP  - 28923
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biočanin, Vladimir and Milošević, Miloš and Trišić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milašin, Jelena and Ilić, Dragan",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
publisher = "Oxford : Elsevier",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
number = "20",
volume = "47",
doi = "10.1016/j.ceramint.2021.07.052",
pages = "28913-28923"
}

Antonijević, Đ., Despotović, A., Biočanin, V., Milošević, M., Trišić, D., Lazović, V., Zogović, N., Milašin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International
Oxford : Elsevier., 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biočanin V, Milošević M, Trišić D, Lazović V, Zogović N, Milašin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biočanin, Vladimir, Milošević, Miloš, Trišić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milašin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biočanin, Vladimir
AU  - Milošević, Miloš
AU  - Trišić, Dijana
AU  - Lazović, Vladimir
AU  - Zogović, Nevena
AU  - Milašin, Jelena
AU  - Ilić, Dragan
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0272884221020794
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4445
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
PB  - Oxford: Elsevier Ltd
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
IS  - 20
VL  - 47
DO  - 10.1016/j.ceramint.2021.07.052
SP  - 28913
EP  - 28923
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biočanin, Vladimir and Milošević, Miloš and Trišić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milašin, Jelena and Ilić, Dragan",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
publisher = "Oxford: Elsevier Ltd",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
number = "20",
volume = "47",
doi = "10.1016/j.ceramint.2021.07.052",
pages = "28913-28923"
}

Antonijević, Đ., Despotović, A., Biočanin, V., Milošević, M., Trišić, D., Lazović, V., Zogović, N., Milašin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International
Oxford: Elsevier Ltd., 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biočanin V, Milošević M, Trišić D, Lazović V, Zogović N, Milašin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biočanin, Vladimir, Milošević, Miloš, Trišić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milašin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .