TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Stepanović, Ana
AU  - Terzić Jovanović, Nataša
AU  - Korać, Aleksandra
AU  - Zlatović, Mario
AU  - Nikolić, Igor
AU  - Opsenica, Igor
AU  - Pešić, Milica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6664
AB  - Two novel hybrid compounds, CON1 and CON2, have been developed by combining sclareol (SC) and doxorubicin (DOX) into a single molecular entity. These hybrid compounds have a 1:1 molar ratio of covalently linked SC and DOX. They have demonstrated promising anticancer properties, especially in glioblastoma cells, and have also shown potential in treating multidrug-resistant (MDR) cancer cells that express the P-glycoprotein (P-gp) membrane transporter. CON1 and CON2 form nanoparticles, as confirmed by Zetasizer, transmission electron microscopy (TEM), and chemical modeling. TEM also showed that CON1 and CON2 can be found in glioblastoma cells, specifically in the cytoplasm, different organelles, nucleus, and nucleolus. To examine the anticancer properties, the U87 glioblastoma cell line, and its corresponding multidrug-resistant U87-TxR cell line, as well as patient-derived astrocytoma grade 3 cells (ASC), were used, while normal human lung fibroblasts were used to determine the selectivity. CON1 and CON2 exhibited better resistance and selectivity profiles than DOX, showing less cytotoxicity, as evidenced by real-time cell analysis, DNA damage determination, cell death induction, mitochondrial respiration, and mitochondrial membrane depolarization studies. Cell cycle analysis and the β-galactosidase activity assay suggested that glioblastoma cells die by senescence following CON1 treatment. Overall, CON1 and CON2 showed great potential as they have better anticancer features than DOX. They are promising candidates for additional preclinical and clinical studies on glioblastoma.
PB  - Elsevier Masson SAS
T2  - Biomedicine & Pharmacotherapy
T1  - Novel hybrid compounds of sclareol and doxorubicin as potential anticancer nanotherapy for glioblastoma
VL  - 174
DO  - 10.1016/j.biopha.2024.116496
SP  - 116496
ER  - 

@article{
author = "Stepanović, Ana and Terzić Jovanović, Nataša and Korać, Aleksandra and Zlatović, Mario and Nikolić, Igor and Opsenica, Igor and Pešić, Milica",
year = "2024",
abstract = "Two novel hybrid compounds, CON1 and CON2, have been developed by combining sclareol (SC) and doxorubicin (DOX) into a single molecular entity. These hybrid compounds have a 1:1 molar ratio of covalently linked SC and DOX. They have demonstrated promising anticancer properties, especially in glioblastoma cells, and have also shown potential in treating multidrug-resistant (MDR) cancer cells that express the P-glycoprotein (P-gp) membrane transporter. CON1 and CON2 form nanoparticles, as confirmed by Zetasizer, transmission electron microscopy (TEM), and chemical modeling. TEM also showed that CON1 and CON2 can be found in glioblastoma cells, specifically in the cytoplasm, different organelles, nucleus, and nucleolus. To examine the anticancer properties, the U87 glioblastoma cell line, and its corresponding multidrug-resistant U87-TxR cell line, as well as patient-derived astrocytoma grade 3 cells (ASC), were used, while normal human lung fibroblasts were used to determine the selectivity. CON1 and CON2 exhibited better resistance and selectivity profiles than DOX, showing less cytotoxicity, as evidenced by real-time cell analysis, DNA damage determination, cell death induction, mitochondrial respiration, and mitochondrial membrane depolarization studies. Cell cycle analysis and the β-galactosidase activity assay suggested that glioblastoma cells die by senescence following CON1 treatment. Overall, CON1 and CON2 showed great potential as they have better anticancer features than DOX. They are promising candidates for additional preclinical and clinical studies on glioblastoma.",
publisher = "Elsevier Masson SAS",
journal = "Biomedicine & Pharmacotherapy",
title = "Novel hybrid compounds of sclareol and doxorubicin as potential anticancer nanotherapy for glioblastoma",
volume = "174",
doi = "10.1016/j.biopha.2024.116496",
pages = "116496"
}

Stepanović, A., Terzić Jovanović, N., Korać, A., Zlatović, M., Nikolić, I., Opsenica, I.,& Pešić, M.. (2024). Novel hybrid compounds of sclareol and doxorubicin as potential anticancer nanotherapy for glioblastoma. in Biomedicine & Pharmacotherapy
Elsevier Masson SAS., 174, 116496.
https://doi.org/10.1016/j.biopha.2024.116496

Stepanović A, Terzić Jovanović N, Korać A, Zlatović M, Nikolić I, Opsenica I, Pešić M. Novel hybrid compounds of sclareol and doxorubicin as potential anticancer nanotherapy for glioblastoma. in Biomedicine & Pharmacotherapy. 2024;174:116496.
doi:10.1016/j.biopha.2024.116496 .

Stepanović, Ana, Terzić Jovanović, Nataša, Korać, Aleksandra, Zlatović, Mario, Nikolić, Igor, Opsenica, Igor, Pešić, Milica, "Novel hybrid compounds of sclareol and doxorubicin as potential anticancer nanotherapy for glioblastoma" in Biomedicine & Pharmacotherapy, 174 (2024):116496,
https://doi.org/10.1016/j.biopha.2024.116496 . .

TY  - JOUR
AU  - Jevtić, Ivana I.
AU  - Suručić, Relja V.
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Kostić-Rajačić, Slađana V.
AU  - Andrić, Deana
AU  - Penjišević, Jelena Z.
PY  - 2024
UR  - https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4662547
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6648
AB  - Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T2  - Bioorganic & Medicinal Chemistry
T1  - Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study
VL  - 101
DO  - 10.1016/j.bmc.2024.117649
SP  - 117649
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6648
ER  - 

@article{
author = "Jevtić, Ivana I. and Suručić, Relja V. and Tovilović-Kovačević, Gordana and Zogović, Nevena and Kostić-Rajačić, Slađana V. and Andrić, Deana and Penjišević, Jelena Z.",
year = "2024",
abstract = "Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Bioorganic & Medicinal Chemistry",
title = "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study",
volume = "101",
doi = "10.1016/j.bmc.2024.117649",
pages = "117649",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6648"
}

Jevtić, I. I., Suručić, R. V., Tovilović-Kovačević, G., Zogović, N., Kostić-Rajačić, S. V., Andrić, D.,& Penjišević, J. Z.. (2024). Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 101, 117649.
https://doi.org/10.1016/j.bmc.2024.117649
https://hdl.handle.net/21.15107/rcub_ibiss_6648

Jevtić II, Suručić RV, Tovilović-Kovačević G, Zogović N, Kostić-Rajačić SV, Andrić D, Penjišević JZ. Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry. 2024;101:117649.
doi:10.1016/j.bmc.2024.117649
https://hdl.handle.net/21.15107/rcub_ibiss_6648 .

Jevtić, Ivana I., Suručić, Relja V., Tovilović-Kovačević, Gordana, Zogović, Nevena, Kostić-Rajačić, Slađana V., Andrić, Deana, Penjišević, Jelena Z., "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study" in Bioorganic & Medicinal Chemistry, 101 (2024):117649,
https://doi.org/10.1016/j.bmc.2024.117649 .,
https://hdl.handle.net/21.15107/rcub_ibiss_6648 .

TY  - JOUR
AU  - Jevtić, Ivana I.
AU  - Suručić, Relja V.
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Kostić-Rajačić, Slađana V.
AU  - Andrić, Deana
AU  - Penjišević, Jelena Z.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6629
UR  - https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4662547
AB  - Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.
PB  - Amsterdam: Elsevier Ltd
T2  - Bioorganic & Medicinal Chemistry
T1  - Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study
VL  - 101
DO  - 10.1016/j.bmc.2024.117649
SP  - 117649
ER  - 

@article{
author = "Jevtić, Ivana I. and Suručić, Relja V. and Tovilović-Kovačević, Gordana and Zogović, Nevena and Kostić-Rajačić, Slađana V. and Andrić, Deana and Penjišević, Jelena Z.",
year = "2024",
abstract = "Simple and scalable synthetic approach was used for the preparation of thirteen novel tacrine derivatives consisting of tacrine and N-aryl-piperidine-4-carboxamide moiety connected by a five-methylene group linker. An anti-Alzheimer disease (AD) potential of newly designed tacrine derivatives was evaluated against two important AD targets, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In vitro pharmacological evaluation showed strong ChE inhibitory activity of all compounds, with IC50 values ranging from 117.5 to 455 nM for AChE and 34 to 324 nM for BuChE. As a representative of the series with the best cytotoxicity / ChE inhibitory activity ratio, expressed as the selectivity index (SI), 2-chlorobenzoyl derivative demonstrated mixed-type inhibition on AChE and BuChE, suggesting binding to both CAS and PAS of the enzymes. It also exhibited antioxidant capacity and neuroprotective potential against amyloid-β (Aβ) toxicity in the culture of neuron-like cells. In-depth computational analysis corroborated well with in vitro ChE inhibition, illuminating that all compounds exhibit significant potential in targeting both enzymes. Molecular dynamics (MD) simulations revealed that 2-chlorobenzoyl derivative, created complexes with AChE and BuChE that demonstrated sufficient stability throughout the observed MD simulation. Computationally predicted ADME properties indicated that these compounds should have good blood–brain barrier (BBB) permeability, an important factor for CNS-targeting drugs. Overall, all tested compounds showed promising pharmacological behavior, highlighting the multi-target potential of 2- chlorobenzoyl derivative which should be further investigated as a new lead in the drug development process.",
publisher = "Amsterdam: Elsevier Ltd",
journal = "Bioorganic & Medicinal Chemistry",
title = "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study",
volume = "101",
doi = "10.1016/j.bmc.2024.117649",
pages = "117649"
}

Jevtić, I. I., Suručić, R. V., Tovilović-Kovačević, G., Zogović, N., Kostić-Rajačić, S. V., Andrić, D.,& Penjišević, J. Z.. (2024). Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry
Amsterdam: Elsevier Ltd., 101, 117649.
https://doi.org/10.1016/j.bmc.2024.117649

Jevtić II, Suručić RV, Tovilović-Kovačević G, Zogović N, Kostić-Rajačić SV, Andrić D, Penjišević JZ. Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study. in Bioorganic & Medicinal Chemistry. 2024;101:117649.
doi:10.1016/j.bmc.2024.117649 .

Jevtić, Ivana I., Suručić, Relja V., Tovilović-Kovačević, Gordana, Zogović, Nevena, Kostić-Rajačić, Slađana V., Andrić, Deana, Penjišević, Jelena Z., "Multi-target potential of newly designed tacrine-derived cholinesterase inhibitors: Synthesis, computational and pharmacological study" in Bioorganic & Medicinal Chemistry, 101 (2024):117649,
https://doi.org/10.1016/j.bmc.2024.117649 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Ljujić, Jovana
AU  - Ćosić, Tatjana
AU  - Stupar, Sofija
AU  - Milutinović, Milica
AU  - Savić, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6632
AB  - β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030353
SP  - 353
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Ljujić, Jovana and Ćosić, Tatjana and Stupar, Sofija and Milutinović, Milica and Savić, Jelena",
year = "2024",
abstract = "β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils
(FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation
method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and
monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups
occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds
from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation.
Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from
both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology
and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the
Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the
developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of
CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic
efficacy of treated larvae remained in line with that of the control larvae. Future investigations will
focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and
potential application.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation",
number = "3",
volume = "14",
doi = "10.3390/biom14030353",
pages = "353"
}

Devrnja, N., Anđelković, B., Ljujić, J., Ćosić, T., Stupar, S., Milutinović, M.,& Savić, J.. (2024). Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules
Basel: MDPI., 14(3), 353.
https://doi.org/10.3390/biom14030353

Devrnja N, Anđelković B, Ljujić J, Ćosić T, Stupar S, Milutinović M, Savić J. Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation. in Biomolecules. 2024;14(3):353.
doi:10.3390/biom14030353 .

Devrnja, Nina, Anđelković, Boban, Ljujić, Jovana, Ćosić, Tatjana, Stupar, Sofija, Milutinović, Milica, Savić, Jelena, "Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation" in Biomolecules, 14, no. 3 (2024):353,
https://doi.org/10.3390/biom14030353 . .

TY  - JOUR
AU  - Jakovljević, Danijel
AU  - Nikolić, Milan
AU  - Jovanović, Vesna
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić-Kokić, Aleksandra
AU  - Novaković, Emilija
AU  - Miljević, Čedo
AU  - Milovanovć, Maja
AU  - Blagojević, Duško
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6510
AB  - Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood
IS  - 1
VL  - 17
DO  - 10.3390/ph17010130
SP  - 130
ER  - 

@article{
author = "Jakovljević, Danijel and Nikolić, Milan and Jovanović, Vesna and Vidonja Uzelac, Teodora and Nikolić-Kokić, Aleksandra and Novaković, Emilija and Miljević, Čedo and Milovanovć, Maja and Blagojević, Duško",
year = "2024",
abstract = "Background: Epilepsy is a chronic brain disease affecting millions of people worldwide,
but little is known about the impact of anti-seizure medications on redox homeostasis. Methods:
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in
monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase,
catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin
content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and
total glutathione in the plasma of epilepsy patients and drug-naïve patients. Results: The results
showed that lamotrigine therapy led to lower superoxide dismutase activity (p < 0.005) and lower
concentrations of total thiols (p < 0.01) and lipid peroxides (p < 0.01) compared to controls. On the
other hand, therapy with carbamazepine increased nitrite levels (p < 0.01) but reduced superoxide
dismutase activity (p < 0.005). In the valproate group, only a decrease in catalase activity was observed
(p < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in
erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls
were separated from all others. Conclusions: Monotherapy with anti-seizure medications discretely
alters redox homeostasis, followed by distinct relationships between antioxidant components.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood",
number = "1",
volume = "17",
doi = "10.3390/ph17010130",
pages = "130"
}

Jakovljević, D., Nikolić, M., Jovanović, V., Vidonja Uzelac, T., Nikolić-Kokić, A., Novaković, E., Miljević, Č., Milovanovć, M.,& Blagojević, D.. (2024). Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals
Basel: MDPI., 17(1), 130.
https://doi.org/10.3390/ph17010130

Jakovljević D, Nikolić M, Jovanović V, Vidonja Uzelac T, Nikolić-Kokić A, Novaković E, Miljević Č, Milovanovć M, Blagojević D. Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood. in Pharmaceuticals. 2024;17(1):130.
doi:10.3390/ph17010130 .

Jakovljević, Danijel, Nikolić, Milan, Jovanović, Vesna, Vidonja Uzelac, Teodora, Nikolić-Kokić, Aleksandra, Novaković, Emilija, Miljević, Čedo, Milovanovć, Maja, Blagojević, Duško, "Influence of Long-Term Anti-Seizure Medications on Redox Parameters in Human Blood" in Pharmaceuticals, 17, no. 1 (2024):130,
https://doi.org/10.3390/ph17010130 . .

TY  - DATA
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina
AU  - Ljujić, Jovana
AU  - Mitić, Zorica
AU  - Nikolić, Biljana
AU  - Marin, Petar
AU  - Tešević, Vele
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6490
AB  - We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6490
ER  - 

@misc{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina and Ljujić, Jovana and Mitić, Zorica and Nikolić, Biljana and Marin, Petar and Tešević, Vele",
year = "2024",
abstract = "We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6490"
}

Milanović, S., Milenković, I., Lazarević, J., Todosijević, M., Ljujić, J., Mitić, Z., Nikolić, B., Marin, P.,& Tešević, V.. (2024). Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6490

Milanović S, Milenković I, Lazarević J, Todosijević M, Ljujić J, Mitić Z, Nikolić B, Marin P, Tešević V. Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina, Ljujić, Jovana, Mitić, Zorica, Nikolić, Biljana, Marin, Petar, Tešević, Vele, "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Krstić, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6557
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - Basel: MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
IS  - 24
VL  - 12
DO  - 10.3390/plants12244181
SP  - 4181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan and Krstić, Gordana",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
number = "24",
volume = "12",
doi = "10.3390/plants12244181",
pages = "4181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S.,& Krstić, G.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
Basel: MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, Krstić, Gordana, "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - CONF
AU  - Opsenica, Igor
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Jovanović, Mirna
AU  - Novaković, Miroslav
AU  - Pešić, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6382
AB  - Derivatization of artemisinin, a natural sesquiterpene lactone, and its synthetic analog artesunate, is of significant interest in medicinal chemistry due to their versatile biological activity, including antimalarial and anticancer. The importance of the pyrimidine scaffold in medicinal chemistry is evidenced by its presence in many natural products and approved drugs, as well as in numerous biologically active compounds.
In this study, we report the synthesis of several novel hybrid molecules comprising two pharmacophores
(artesunic acid and pyrimidine scaffold) and their activity against sensitive and multidrug‐resistant (MDR)
human non‐small cell lung carcinoma (NSCLC) cells. The synthesis of novel artemisinin-pyrimidine hybrid
molecules was accomplished via amide bond formation between artesunic acid and pyrimidine derivatives. A lead compound was identified through structure activity relationship (SAR) studies. Several hybrids were capable of evading the MDR phenotype, increasing the sensitivity of MDR NSCLC cells toward doxorubicin and displayed inhibitory activity against P-glycoprotein.
PB  - Cambridge: Royal Society of Chemistry
C3  - Book of abstracts: 22nd European Symposium on Organic Chemistry Ghent: ESOC23GHENT; 2023 Jul 9-13; Ghent, Belgium
T1  - Synthesis of Novel Artemisinin Derivatives With Potent Anticancer Activities Against Multidrug-resistant Cancer Cells
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6382
ER  - 

@conference{
author = "Opsenica, Igor and Koračak, Ljiljana and Lupšić, Ema and Jovanović, Mirna and Novaković, Miroslav and Pešić, Milica",
year = "2023",
abstract = "Derivatization of artemisinin, a natural sesquiterpene lactone, and its synthetic analog artesunate, is of significant interest in medicinal chemistry due to their versatile biological activity, including antimalarial and anticancer. The importance of the pyrimidine scaffold in medicinal chemistry is evidenced by its presence in many natural products and approved drugs, as well as in numerous biologically active compounds.
In this study, we report the synthesis of several novel hybrid molecules comprising two pharmacophores
(artesunic acid and pyrimidine scaffold) and their activity against sensitive and multidrug‐resistant (MDR)
human non‐small cell lung carcinoma (NSCLC) cells. The synthesis of novel artemisinin-pyrimidine hybrid
molecules was accomplished via amide bond formation between artesunic acid and pyrimidine derivatives. A lead compound was identified through structure activity relationship (SAR) studies. Several hybrids were capable of evading the MDR phenotype, increasing the sensitivity of MDR NSCLC cells toward doxorubicin and displayed inhibitory activity against P-glycoprotein.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "Book of abstracts: 22nd European Symposium on Organic Chemistry Ghent: ESOC23GHENT; 2023 Jul 9-13; Ghent, Belgium",
title = "Synthesis of Novel Artemisinin Derivatives With Potent Anticancer Activities Against Multidrug-resistant Cancer Cells",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6382"
}

Opsenica, I., Koračak, L., Lupšić, E., Jovanović, M., Novaković, M.,& Pešić, M.. (2023). Synthesis of Novel Artemisinin Derivatives With Potent Anticancer Activities Against Multidrug-resistant Cancer Cells. in Book of abstracts: 22nd European Symposium on Organic Chemistry Ghent: ESOC23GHENT; 2023 Jul 9-13; Ghent, Belgium
Cambridge: Royal Society of Chemistry., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_6382

Opsenica I, Koračak L, Lupšić E, Jovanović M, Novaković M, Pešić M. Synthesis of Novel Artemisinin Derivatives With Potent Anticancer Activities Against Multidrug-resistant Cancer Cells. in Book of abstracts: 22nd European Symposium on Organic Chemistry Ghent: ESOC23GHENT; 2023 Jul 9-13; Ghent, Belgium. 2023;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_6382 .

Opsenica, Igor, Koračak, Ljiljana, Lupšić, Ema, Jovanović, Mirna, Novaković, Miroslav, Pešić, Milica, "Synthesis of Novel Artemisinin Derivatives With Potent Anticancer Activities Against Multidrug-resistant Cancer Cells" in Book of abstracts: 22nd European Symposium on Organic Chemistry Ghent: ESOC23GHENT; 2023 Jul 9-13; Ghent, Belgium (2023):213,
https://hdl.handle.net/21.15107/rcub_ibiss_6382 .

TY  - CONF
AU  - Koračak, Ljiljana K.
AU  - Lupšić, Ema
AU  - Jovanović, Mirna
AU  - Novaković, Miroslav
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6383
AB  - Značaj artemizinina i njegovih derivata se ogleda u biološkoj aktivnosti jer osim što su
našli primjenu kao efikasni lijekovi za liječenje malarije, pokazuju i antitumorsku
aktivnost. Pirimidinsko jezgro je važno zbog prisustva ovog strukturnog motiva u
prirodnim proizvodima, u odobrenim lijekovima, ali i u biološki aktivnim molekulima. U
okviru ovog istraživanja prijavljena je sinteza novih hibridnih molekula dobijenih
povezivanjem dvije farmakofore, kao i njihova antitumorska aktivnost na rezistentnim i
osjetljivim ćelijama nesitnoćelijskog karcinoma pluća.
AB  - In addition to being used for the effective treatment of malaria, artemisinin and derivatives also exhibit anticancer activity. The importance of the pyrimidine scaffold is evidenced by its presence in natural products and approved drugs, as well as in biologically active compounds. In this study, we report the synthesis of novel hybrid molecules comprising two pharmacophores and their activity against sensitive and multidrug‐resistant human non‐small cell lung carcinoma cells.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia
T1  - Sinteza novih derivata artemizinina sa antitumorskom aktivnošću na rezistentne ćelije raka
T1  - Synthesis of novel artemisinin derivatives with anticancer activity against multidrug-resistant cancer cells
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6383
ER  - 

@conference{
author = "Koračak, Ljiljana K. and Lupšić, Ema and Jovanović, Mirna and Novaković, Miroslav and Pešić, Milica and Opsenica, Igor M.",
year = "2023",
abstract = "Značaj artemizinina i njegovih derivata se ogleda u biološkoj aktivnosti jer osim što su
našli primjenu kao efikasni lijekovi za liječenje malarije, pokazuju i antitumorsku
aktivnost. Pirimidinsko jezgro je važno zbog prisustva ovog strukturnog motiva u
prirodnim proizvodima, u odobrenim lijekovima, ali i u biološki aktivnim molekulima. U
okviru ovog istraživanja prijavljena je sinteza novih hibridnih molekula dobijenih
povezivanjem dvije farmakofore, kao i njihova antitumorska aktivnost na rezistentnim i
osjetljivim ćelijama nesitnoćelijskog karcinoma pluća., In addition to being used for the effective treatment of malaria, artemisinin and derivatives also exhibit anticancer activity. The importance of the pyrimidine scaffold is evidenced by its presence in natural products and approved drugs, as well as in biologically active compounds. In this study, we report the synthesis of novel hybrid molecules comprising two pharmacophores and their activity against sensitive and multidrug‐resistant human non‐small cell lung carcinoma cells.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia",
title = "Sinteza novih derivata artemizinina sa antitumorskom aktivnošću na rezistentne ćelije raka, Synthesis of novel artemisinin derivatives with anticancer activity against multidrug-resistant cancer cells",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6383"
}

Koračak, L. K., Lupšić, E., Jovanović, M., Novaković, M., Pešić, M.,& Opsenica, I. M.. (2023). Sinteza novih derivata artemizinina sa antitumorskom aktivnošću na rezistentne ćelije raka. in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia
Belgrade: Serbian Chemical Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_6383

Koračak LK, Lupšić E, Jovanović M, Novaković M, Pešić M, Opsenica IM. Sinteza novih derivata artemizinina sa antitumorskom aktivnošću na rezistentne ćelije raka. in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia. 2023;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_6383 .

Koračak, Ljiljana K., Lupšić, Ema, Jovanović, Mirna, Novaković, Miroslav, Pešić, Milica, Opsenica, Igor M., "Sinteza novih derivata artemizinina sa antitumorskom aktivnošću na rezistentne ćelije raka" in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia (2023):70,
https://hdl.handle.net/21.15107/rcub_ibiss_6383 .

TY  - CONF
AU  - Sibinčić, Nikolina
AU  - Krstić Ristivojević, Maja
AU  - Stojanović, Marijana
AU  - Mladenović Stokanić, Maja
AU  - Vasović, Tamara
AU  - Ćirković Veličković, Tanja
AU  - Stojadinović, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6318
AB  - The SARS-CoV-2 nucleocapsid (N) protein plays a significant role in the coronavirus life cycle and participates in a variety of critical events following viral invasion1. In infected patients, high titers of immunoglobulin G (IgG) targeting N protein were detected and correlated with the clinical course of the disease2. Therefore, N protein and anti-N protein IgGs were recognized as important diagnostic indicators of COVID-19 infection in serological and quick antigen tests3. In this study, we optimized the expression of the recombinant form of SARS-CoV-2 N protein in a mammalian cell line HEK293T by comparing the transfection efficiency between Polyethylenimine (PEI) and Calcium Phosphate (CaP) DNA-complexing agents. Transfection potency was tested at different cell confluence and passage number, in several cell culture media, pre-transfection and post-transfection media change and in conditions of reduced serum. Chloroquine and glycerol treatments were included to enhance transfection efficiency as they might inhibit DNA degradation in lysosomes or increase membrane permeability. Protein expression was monitored in cell supernatants up to 7 days post-transfection in dot-bot and Western blot using anti-N protein antibodies. Both transfection methods have shown moderate to relatively high transfection efficiency dependent on the applied conditions, making them affordable and easy to use techniques for recombinant N protein production on a small-scale in adherent mammalian systems. PEI acts as a good delivery system regardless of the presence of the fetal bovine serum (FBS), while CaP transfection is more dependent on the presence of FBS which in turn favors N protein degradation. However, we have optimized both methods to achieve optimal expression of unfragmented N-protein in serum-free conditions. Apart from setting up a cost-effective platform for expression of N protein in mammalian cells, we plan on investigating the mechanisms behind the PEI and CaP non-viral gene delivery systems as there are still some uncertainties in the scientific community.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Expression of recombinant SARS-CoV-2 nucleocapsid protein in mammalian cells
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6318
ER  - 

@conference{
author = "Sibinčić, Nikolina and Krstić Ristivojević, Maja and Stojanović, Marijana and Mladenović Stokanić, Maja and Vasović, Tamara and Ćirković Veličković, Tanja and Stojadinović, Marija",
year = "2023",
abstract = "The SARS-CoV-2 nucleocapsid (N) protein plays a significant role in the coronavirus life cycle and participates in a variety of critical events following viral invasion1. In infected patients, high titers of immunoglobulin G (IgG) targeting N protein were detected and correlated with the clinical course of the disease2. Therefore, N protein and anti-N protein IgGs were recognized as important diagnostic indicators of COVID-19 infection in serological and quick antigen tests3. In this study, we optimized the expression of the recombinant form of SARS-CoV-2 N protein in a mammalian cell line HEK293T by comparing the transfection efficiency between Polyethylenimine (PEI) and Calcium Phosphate (CaP) DNA-complexing agents. Transfection potency was tested at different cell confluence and passage number, in several cell culture media, pre-transfection and post-transfection media change and in conditions of reduced serum. Chloroquine and glycerol treatments were included to enhance transfection efficiency as they might inhibit DNA degradation in lysosomes or increase membrane permeability. Protein expression was monitored in cell supernatants up to 7 days post-transfection in dot-bot and Western blot using anti-N protein antibodies. Both transfection methods have shown moderate to relatively high transfection efficiency dependent on the applied conditions, making them affordable and easy to use techniques for recombinant N protein production on a small-scale in adherent mammalian systems. PEI acts as a good delivery system regardless of the presence of the fetal bovine serum (FBS), while CaP transfection is more dependent on the presence of FBS which in turn favors N protein degradation. However, we have optimized both methods to achieve optimal expression of unfragmented N-protein in serum-free conditions. Apart from setting up a cost-effective platform for expression of N protein in mammalian cells, we plan on investigating the mechanisms behind the PEI and CaP non-viral gene delivery systems as there are still some uncertainties in the scientific community.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Expression of recombinant SARS-CoV-2 nucleocapsid protein in mammalian cells",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6318"
}

Sibinčić, N., Krstić Ristivojević, M., Stojanović, M., Mladenović Stokanić, M., Vasović, T., Ćirković Veličković, T.,& Stojadinović, M.. (2023). Expression of recombinant SARS-CoV-2 nucleocapsid protein in mammalian cells. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6318

Sibinčić N, Krstić Ristivojević M, Stojanović M, Mladenović Stokanić M, Vasović T, Ćirković Veličković T, Stojadinović M. Expression of recombinant SARS-CoV-2 nucleocapsid protein in mammalian cells. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6318 .

Sibinčić, Nikolina, Krstić Ristivojević, Maja, Stojanović, Marijana, Mladenović Stokanić, Maja, Vasović, Tamara, Ćirković Veličković, Tanja, Stojadinović, Marija, "Expression of recombinant SARS-CoV-2 nucleocapsid protein in mammalian cells" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6318 .

TY  - JOUR
AU  - Platanić-Arizanović, Lena
AU  - Gligorijević, Nikola
AU  - Cvijetić, Ilija
AU  - Mijatović, Aleksandar
AU  - Krstić-Ristivojević, Maja
AU  - Minić, Simeon
AU  - Nikolić-Kokić, Aleksandra
AU  - Miljević, Čedo
AU  - Nikolić, Milan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6028
AB  - : Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human
hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching
at different temperatures and data obtained from the van’t Hoff diagram and molecular docking
indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site
for all drugs in the central cavity near αβ interfaces and is dominantly mediated through hydrophobic
forces. The association constants were lower-moderate strength (~104 M−1
), the highest observed
for clozapine (2.2 × 104 M−1 at 25 ◦C). The clozapine binding showed “friendly” effects: increased
α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation.
On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing
ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital
role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the
obtained findings is briefly discussed.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?
IS  - 10
VL  - 24
DO  - 10.3390/ijms24108921
SP  - 8921
ER  - 

@article{
author = "Platanić-Arizanović, Lena and Gligorijević, Nikola and Cvijetić, Ilija and Mijatović, Aleksandar and Krstić-Ristivojević, Maja and Minić, Simeon and Nikolić-Kokić, Aleksandra and Miljević, Čedo and Nikolić, Milan",
year = "2023",
abstract = ": Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human
hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching
at different temperatures and data obtained from the van’t Hoff diagram and molecular docking
indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site
for all drugs in the central cavity near αβ interfaces and is dominantly mediated through hydrophobic
forces. The association constants were lower-moderate strength (~104 M−1
), the highest observed
for clozapine (2.2 × 104 M−1 at 25 ◦C). The clozapine binding showed “friendly” effects: increased
α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation.
On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing
ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital
role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the
obtained findings is briefly discussed.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?",
number = "10",
volume = "24",
doi = "10.3390/ijms24108921",
pages = "8921"
}

Platanić-Arizanović, L., Gligorijević, N., Cvijetić, I., Mijatović, A., Krstić-Ristivojević, M., Minić, S., Nikolić-Kokić, A., Miljević, Č.,& Nikolić, M.. (2023). Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences
Basel: MDPI., 24(10), 8921.
https://doi.org/10.3390/ijms24108921

Platanić-Arizanović L, Gligorijević N, Cvijetić I, Mijatović A, Krstić-Ristivojević M, Minić S, Nikolić-Kokić A, Miljević Č, Nikolić M. Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences. 2023;24(10):8921.
doi:10.3390/ijms24108921 .

Platanić-Arizanović, Lena, Gligorijević, Nikola, Cvijetić, Ilija, Mijatović, Aleksandar, Krstić-Ristivojević, Maja, Minić, Simeon, Nikolić-Kokić, Aleksandra, Miljević, Čedo, Nikolić, Milan, "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?" in International Journal of Molecular Sciences, 24, no. 10 (2023):8921,
https://doi.org/10.3390/ijms24108921 . .

TY  - CONF
AU  - Stojković, Pavle A.
AU  - Stepanović, Ana
AU  - Lupšić, Ema
AU  - Terzić Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5911
AB  - Sklareol, biološki aktivan diterpen, je iskorišćen kao polazna supstanca za sintezu novih hibridnih molekula sa 1,2,4-triazolo[1,5-a]-pirimidinskim jezgrom (Slika 1). Svi derivati sklareola su testirani na ćelijsku liniju ljudskog glioblastoma U 7 i ćelijsku liniju U 7-TxR koja ispoljava višestruku rezistenciju na lekove. Jedinjenja su modifikovala aktivnost P-glikoproteina u sličnoj meri kao P-gp inhibitor treće generacije – tarikvidar. Ispitan je uticaj novih jedinjenja na različite ćelijske procese među kojima su ćelijski ciklus i ćelijska smrt, kao i na koncentraciju reaktivnih kiseoničnih i azotnih vrsta (ROS/RNS) u ćelijama glioblastoma i na potencijal membrane mitohondrija.
AB  - Sclareol, a biologically active diterpenoid, was used as the starting material for the synthesis of novel hybrid molecules containing the 1,2,4-triazolo[1,5-a]-pyrimidine moiety. All sclareol derivatives were tested on human glioblastoma U87 and multi-drug resistant U87-TxR cells. Hybrid compounds decreased P-gp activity to the same extent as a third generation P-gp inhibitor - tariquidar. We examined the effect of novel compounds on various cellular processes including the cell cycle and cell death, as well as their influence on the levels of reactive oxygen and nitrogen species (ROS/RNS) and mitochondrial membrane potential in glioblastoma cells.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia
T1  - Sinteza i citotoksičnost novih derivata sklareola
T1  - Synthesis and cytotoxic activity of novel sclareol derivatives
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5911
ER  - 

@conference{
author = "Stojković, Pavle A. and Stepanović, Ana and Lupšić, Ema and Terzić Jovanović, Nataša and Novaković, Miroslav and Pešić, Milica and Opsenica, Igor M.",
year = "2023",
abstract = "Sklareol, biološki aktivan diterpen, je iskorišćen kao polazna supstanca za sintezu novih hibridnih molekula sa 1,2,4-triazolo[1,5-a]-pirimidinskim jezgrom (Slika 1). Svi derivati sklareola su testirani na ćelijsku liniju ljudskog glioblastoma U 7 i ćelijsku liniju U 7-TxR koja ispoljava višestruku rezistenciju na lekove. Jedinjenja su modifikovala aktivnost P-glikoproteina u sličnoj meri kao P-gp inhibitor treće generacije – tarikvidar. Ispitan je uticaj novih jedinjenja na različite ćelijske procese među kojima su ćelijski ciklus i ćelijska smrt, kao i na koncentraciju reaktivnih kiseoničnih i azotnih vrsta (ROS/RNS) u ćelijama glioblastoma i na potencijal membrane mitohondrija., Sclareol, a biologically active diterpenoid, was used as the starting material for the synthesis of novel hybrid molecules containing the 1,2,4-triazolo[1,5-a]-pyrimidine moiety. All sclareol derivatives were tested on human glioblastoma U87 and multi-drug resistant U87-TxR cells. Hybrid compounds decreased P-gp activity to the same extent as a third generation P-gp inhibitor - tariquidar. We examined the effect of novel compounds on various cellular processes including the cell cycle and cell death, as well as their influence on the levels of reactive oxygen and nitrogen species (ROS/RNS) and mitochondrial membrane potential in glioblastoma cells.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia",
title = "Sinteza i citotoksičnost novih derivata sklareola, Synthesis and cytotoxic activity of novel sclareol derivatives",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5911"
}

Stojković, P. A., Stepanović, A., Lupšić, E., Terzić Jovanović, N., Novaković, M., Pešić, M.,& Opsenica, I. M.. (2023). Sinteza i citotoksičnost novih derivata sklareola. in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia
Belgrade: Serbian Chemical Society., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_5911

Stojković PA, Stepanović A, Lupšić E, Terzić Jovanović N, Novaković M, Pešić M, Opsenica IM. Sinteza i citotoksičnost novih derivata sklareola. in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia. 2023;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_5911 .

Stojković, Pavle A., Stepanović, Ana, Lupšić, Ema, Terzić Jovanović, Nataša, Novaković, Miroslav, Pešić, Milica, Opsenica, Igor M., "Sinteza i citotoksičnost novih derivata sklareola" in Book of Abstracts and Proceedings: 59th meeting of the Serbian Chemical Society; 2023 Jun 1-2; Novi Sad, Serbia (2023):77,
https://hdl.handle.net/21.15107/rcub_ibiss_5911 .

TY  - JOUR
AU  - Stojković, Pavle
AU  - Stepanović, Ana
AU  - Lupšić, Ema
AU  - Terzić Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Pešić, Milica
AU  - Opsenica, Igor M.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5910
AB  - The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo
[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic
properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine
linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f
consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and
11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out
of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 μM. The
concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding
multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and
normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven
compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All
compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a,
12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased
P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its
precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial
membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma
cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress
accompanied by inhibition of mitochondria.
PB  - Academic Press Inc.
T2  - Bioorganic Chemistry
T1  - Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells
VL  - 138
DO  - 10.1016/j.bioorg.2023.106605
SP  - 106605
ER  - 

@article{
author = "Stojković, Pavle and Stepanović, Ana and Lupšić, Ema and Terzić Jovanović, Nataša and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Pešić, Milica and Opsenica, Igor M.",
year = "2023",
abstract = "The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo
[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic
properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine
linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f
consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and
11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out
of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 μM. The
concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding
multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and
normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven
compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All
compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a,
12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased
P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its
precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial
membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma
cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress
accompanied by inhibition of mitochondria.",
publisher = "Academic Press Inc.",
journal = "Bioorganic Chemistry",
title = "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells",
volume = "138",
doi = "10.1016/j.bioorg.2023.106605",
pages = "106605"
}

Stojković, P., Stepanović, A., Lupšić, E., Terzić Jovanović, N., Novaković, M., Nedialkov, P., Trendafilova, A., Pešić, M.,& Opsenica, I. M.. (2023). Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry
Academic Press Inc.., 138, 106605.
https://doi.org/10.1016/j.bioorg.2023.106605

Stojković P, Stepanović A, Lupšić E, Terzić Jovanović N, Novaković M, Nedialkov P, Trendafilova A, Pešić M, Opsenica IM. Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry. 2023;138:106605.
doi:10.1016/j.bioorg.2023.106605 .

Stojković, Pavle, Stepanović, Ana, Lupšić, Ema, Terzić Jovanović, Nataša, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Pešić, Milica, Opsenica, Igor M., "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells" in Bioorganic Chemistry, 138 (2023):106605,
https://doi.org/10.1016/j.bioorg.2023.106605 . .

TY  - JOUR
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Terzić Jovanović, Nataša
AU  - Jovanović, Mirna
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Zlatović, Mario
AU  - Pešić, Milica
AU  - Opsenica, Igor
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5890
AB  - The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.
PB  - Cambridge: Royal Society of Chemistry
T2  - New Journal of Chemistry
T1  - Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells
IS  - 14
VL  - 47
DO  - 10.1039/D3NJ00427A
SP  - 6844
EP  - 6855
ER  - 

@article{
author = "Koračak, Ljiljana and Lupšić, Ema and Terzić Jovanović, Nataša and Jovanović, Mirna and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Zlatović, Mario and Pešić, Milica and Opsenica, Igor",
year = "2023",
abstract = "The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "New Journal of Chemistry",
title = "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells",
number = "14",
volume = "47",
doi = "10.1039/D3NJ00427A",
pages = "6844-6855"
}

Koračak, L., Lupšić, E., Terzić Jovanović, N., Jovanović, M., Novaković, M., Nedialkov, P., Trendafilova, A., Zlatović, M., Pešić, M.,& Opsenica, I.. (2023). Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry
Cambridge: Royal Society of Chemistry., 47(14), 6844-6855.
https://doi.org/10.1039/D3NJ00427A

Koračak L, Lupšić E, Terzić Jovanović N, Jovanović M, Novaković M, Nedialkov P, Trendafilova A, Zlatović M, Pešić M, Opsenica I. Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry. 2023;47(14):6844-6855.
doi:10.1039/D3NJ00427A .

Koračak, Ljiljana, Lupšić, Ema, Terzić Jovanović, Nataša, Jovanović, Mirna, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Zlatović, Mario, Pešić, Milica, Opsenica, Igor, "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells" in New Journal of Chemistry, 47, no. 14 (2023):6844-6855,
https://doi.org/10.1039/D3NJ00427A . .

TY  - JOUR
AU  - Mikavica, Ivana
AU  - Ranđelović, Dragana
AU  - Đorđević, Vladan
AU  - Rakić, Tamara
AU  - Gajić, Gordana
AU  - Mutić, Jelena
PY  - 2023
UR  - https://link.springer.com/10.1007/s11356-022-22110-z
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5092
AB  - The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types-cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.
PB  - Heidelberg: Springer Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types
VL  - 30
DO  - 10.1007/s11356-022-22110-z
SP  - 979
EP  - 995
ER  - 

@article{
author = "Mikavica, Ivana and Ranđelović, Dragana and Đorđević, Vladan and Rakić, Tamara and Gajić, Gordana and Mutić, Jelena",
year = "2023",
abstract = "The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types-cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.",
publisher = "Heidelberg: Springer Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types",
volume = "30",
doi = "10.1007/s11356-022-22110-z",
pages = "979-995"
}

Mikavica, I., Ranđelović, D., Đorđević, V., Rakić, T., Gajić, G.,& Mutić, J.. (2023). Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research
Heidelberg: Springer Heidelberg., 30, 979-995.
https://doi.org/10.1007/s11356-022-22110-z

Mikavica I, Ranđelović D, Đorđević V, Rakić T, Gajić G, Mutić J. Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research. 2023;30:979-995.
doi:10.1007/s11356-022-22110-z .

Mikavica, Ivana, Ranđelović, Dragana, Đorđević, Vladan, Rakić, Tamara, Gajić, Gordana, Mutić, Jelena, "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types" in Environmental Science and Pollution Research, 30 (2023):979-995,
https://doi.org/10.1007/s11356-022-22110-z . .

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar M.
AU  - Gašić, Uroš
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka M.
AU  - Trifković, Jelena Đ.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5162
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Oxford: Elsevier Sci Ltd.
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
DO  - 10.1016/j.foodchem.2022.134184
SP  - 134184
ER  - 

@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar M. and Gašić, Uroš and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka M. and Trifković, Jelena Đ.",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity as one of the most important quality assurances, phenolic profiling by high-performance thin layer chromatography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry) revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Oxford: Elsevier Sci Ltd.",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
doi = "10.1016/j.foodchem.2022.134184",
pages = "134184"
}

Krstić, Đ. D., Ristivojević, P. M., Gašić, U., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Oxford: Elsevier Sci Ltd.., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184

Krstić ĐD, Ristivojević PM, Gašić U, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica DM, Trifković JĐ. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .

Krstić, Đurđa D., Ristivojević, Petar M., Gašić, Uroš, Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka M., Trifković, Jelena Đ., "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6594
AB  - In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.
PB  - Skopje: Macedonian Pharmaceutical Association
C3  - 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
T1  - Anti-melanoma effects of ingenanes isolated from Euphorbia species
DO  - 10.33320/maced.pharm.bull.2022.68.04.006
SP  - 23
EP  - 24
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Jovanović Stojanov, Sofija and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.",
publisher = "Skopje: Macedonian Pharmaceutical Association",
journal = "11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia",
title = "Anti-melanoma effects of ingenanes isolated from Euphorbia species",
doi = "10.33320/maced.pharm.bull.2022.68.04.006",
pages = "23-24"
}

Krstić, G., Jadranin, M., Jovanović Stojanov, S., Pešić, M., Tešević, V.,& Milosavljević, S.. (2022). Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
Skopje: Macedonian Pharmaceutical Association., 23-24.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006

Krstić G, Jadranin M, Jovanović Stojanov S, Pešić M, Tešević V, Milosavljević S. Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia. 2022;:23-24.
doi:10.33320/maced.pharm.bull.2022.68.04.006 .

Krstić, Gordana, Jadranin, Milka, Jovanović Stojanov, Sofija, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, "Anti-melanoma effects of ingenanes isolated from Euphorbia species" in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia (2022):23-24,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022004757
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4983
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
DO  - 10.1016/j.indcrop.2022.114992
SP  - 114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites , such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus . Histochemical staining of leaves' trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus , grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) and two Gram-positive bacteria ( Bacillus cereus and Micrococcus flavus ). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent -3 β -acetoxy-13- epi -manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent -3 β -acetoxy-13- epi -manoyl oxide and its precursor ent -3 β -hydroxy-13- epi -manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
doi = "10.1016/j.indcrop.2022.114992",
pages = "114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products
Elsevier B.V.., 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković B, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić U, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban, Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš, Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš
AU  - Đurović, Dejan
AU  - Tešić, Živoslav
AU  - Fotirić Akšić, Milica
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9653822
UR  - https://www.mdpi.com/1420-3049/27/21/7651
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5239
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - Basel: MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
IS  - 21
VL  - 27
DO  - 10.3390/molecules27217651
SP  - 7651
ER  - 

@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš and Đurović, Dejan and Tešić, Živoslav and Fotirić Akšić, Milica and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
number = "21",
volume = "27",
doi = "10.3390/molecules27217651",
pages = "7651"
}

Horvacki, N., Andrić, F., Gašić, U., Đurović, D., Tešić, Ž., Fotirić Akšić, M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
Basel: MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651

Horvacki N, Andrić F, Gašić U, Đurović D, Tešić Ž, Fotirić Akšić M, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš, Đurović, Dejan, Tešić, Živoslav, Fotirić Akšić, Milica, Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .

TY  - JOUR
AU  - Trifunović, Sara
AU  - Smiljanić, Katarina
AU  - Sickmann, Albert
AU  - Solari, Fiorella A.
AU  - Kolarević, Stoimir
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
PY  - 2022
UR  - https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-022-02102-w
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9285873
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5082
AB  - BACKGROUND Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. METHODS In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. RESULTS E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. CONCLUSIONS Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.
PB  - London: BMC
T2  - Respiratory Research
T1  - Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.
IS  - 1
VL  - 23
DO  - 10.1186/s12931-022-02102-w
SP  - 191
ER  - 

@article{
author = "Trifunović, Sara and Smiljanić, Katarina and Sickmann, Albert and Solari, Fiorella A. and Kolarević, Stoimir and Divac Rankov, Aleksandra and Ljujić, Mila",
year = "2022",
abstract = "BACKGROUND Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. METHODS In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. RESULTS E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. CONCLUSIONS Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.",
publisher = "London: BMC",
journal = "Respiratory Research",
title = "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.",
number = "1",
volume = "23",
doi = "10.1186/s12931-022-02102-w",
pages = "191"
}

Trifunović, S., Smiljanić, K., Sickmann, A., Solari, F. A., Kolarević, S., Divac Rankov, A.,& Ljujić, M.. (2022). Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.. in Respiratory Research
London: BMC., 23(1), 191.
https://doi.org/10.1186/s12931-022-02102-w

Trifunović S, Smiljanić K, Sickmann A, Solari FA, Kolarević S, Divac Rankov A, Ljujić M. Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.. in Respiratory Research. 2022;23(1):191.
doi:10.1186/s12931-022-02102-w .

Trifunović, Sara, Smiljanić, Katarina, Sickmann, Albert, Solari, Fiorella A., Kolarević, Stoimir, Divac Rankov, Aleksandra, Ljujić, Mila, "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells." in Respiratory Research, 23, no. 1 (2022):191,
https://doi.org/10.1186/s12931-022-02102-w . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav
AU  - Vovk, Irena
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941463/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9263698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5080
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.
VL  - 9
DO  - 10.3389/fnut.2022.941463
SP  - 941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.",
volume = "9",
doi = "10.3389/fnut.2022.941463",
pages = "941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić U, Baošić R, Joksimović K, Pezo L, Tešić Ž, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš, Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav, Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia." in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .