TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Stanojević, Olja
AU  - Janakiev, Tamara
AU  - Tadić, Vojin
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://www.pjoes.com/doi/10.15244/pjoes/81687
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3255
AB  - In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
T2  - Polish Journal of Environmental Studies
T1  - Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/81687
SP  - 811
EP  - 822
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Stanojević, Olja and Janakiev, Tamara and Tadić, Vojin and Stanković, Slaviša",
year = "2019",
abstract = "In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.",
journal = "Polish Journal of Environmental Studies",
title = "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II",
number = "2",
volume = "28",
doi = "10.15244/pjoes/81687",
pages = "811-822"
}

Radulović, O., Petrić, M., Raspor, M., Stanojević, O., Janakiev, T., Tadić, V.,& Stanković, S.. (2019). Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies, 28(2), 811-822.
https://doi.org/10.15244/pjoes/81687

Radulović O, Petrić M, Raspor M, Stanojević O, Janakiev T, Tadić V, Stanković S. Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies. 2019;28(2):811-822.
doi:10.15244/pjoes/81687 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Stanojević, Olja, Janakiev, Tamara, Tadić, Vojin, Stanković, Slaviša, "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II" in Polish Journal of Environmental Studies, 28, no. 2 (2019):811-822,
https://doi.org/10.15244/pjoes/81687 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Tadić, Vojin
AU  - Jovanović, Petar
AU  - Zečević, Vladan
PY  - 2019
UR  - http://www.journalssystem.com/pjoes/Assessment-of-in-vitro-multiplication-of-Lemna-minor-in-the-presence-of-phenol-plant,84921,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3251
AB  - The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.
T2  - Polish Journal of Environmental Studies
T1  - Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/84921
SP  - 803
EP  - 809
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Tadić, Vojin and Jovanović, Petar and Zečević, Vladan",
year = "2019",
abstract = "The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.",
journal = "Polish Journal of Environmental Studies",
title = "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I",
number = "2",
volume = "28",
doi = "10.15244/pjoes/84921",
pages = "803-809"
}

Radulović, O., Petrić, M., Raspor, M., Tadić, V., Jovanović, P.,& Zečević, V.. (2019). Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies, 28(2), 803-809.
https://doi.org/10.15244/pjoes/84921

Radulović O, Petrić M, Raspor M, Tadić V, Jovanović P, Zečević V. Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies. 2019;28(2):803-809.
doi:10.15244/pjoes/84921 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Tadić, Vojin, Jovanović, Petar, Zečević, Vladan, "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I" in Polish Journal of Environmental Studies, 28, no. 2 (2019):803-809,
https://doi.org/10.15244/pjoes/84921 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Uzelac, Branka
AU  - Milošević, Snežana
AU  - Vujčić, Zoran
AU  - Stevanović, Jasmina
AU  - Tadić, Jovan
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2956
AB  - We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
T2  - Scientia Horticulturae
T1  - Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1
VL  - 231
DO  - 10.1016/j.scienta.2017.12.025
SP  - 210
EP  - 218
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Uzelac, Branka and Milošević, Snežana and Vujčić, Zoran and Stevanović, Jasmina and Tadić, Jovan",
year = "2018",
abstract = "We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
journal = "Scientia Horticulturae",
title = "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1",
volume = "231",
doi = "10.1016/j.scienta.2017.12.025",
pages = "210-218"
}

Tadić, V., Petrić, M., Uzelac, B., Milošević, S., Vujčić, Z., Stevanović, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae, 231, 210-218.
https://doi.org/10.1016/j.scienta.2017.12.025

Tadić V, Petrić M, Uzelac B, Milošević S, Vujčić Z, Stevanović J, Tadić J. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae. 2018;231:210-218.
doi:10.1016/j.scienta.2017.12.025 .

Tadić, Vojin, Petrić, Marija, Uzelac, Branka, Milošević, Snežana, Vujčić, Zoran, Stevanović, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1" in Scientia Horticulturae, 231 (2018):210-218,
https://doi.org/10.1016/j.scienta.2017.12.025 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Tadić, Jovan
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Prodanović, Olivera
AU  - Ćosić, Tatjana
AU  - Vujčić, Zoran
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307471
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2955
AB  - In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL−1 during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of proline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.
T2  - Scientia Horticulturae
T1  - Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2
VL  - 232
DO  - 10.1016/j.scienta.2017.12.024
SP  - 71
EP  - 83
ER  - 

@article{
author = "Tadić, Vojin and Tadić, Jovan and Milošević, Snežana and Cingel, Aleksandar and Prodanović, Olivera and Ćosić, Tatjana and Vujčić, Zoran",
year = "2018",
abstract = "In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL−1 during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of proline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.",
journal = "Scientia Horticulturae",
title = "Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2",
volume = "232",
doi = "10.1016/j.scienta.2017.12.024",
pages = "71-83"
}

Tadić, V., Tadić, J., Milošević, S., Cingel, A., Prodanović, O., Ćosić, T.,& Vujčić, Z.. (2018). Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2. in Scientia Horticulturae, 232, 71-83.
https://doi.org/10.1016/j.scienta.2017.12.024

Tadić V, Tadić J, Milošević S, Cingel A, Prodanović O, Ćosić T, Vujčić Z. Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2. in Scientia Horticulturae. 2018;232:71-83.
doi:10.1016/j.scienta.2017.12.024 .

Tadić, Vojin, Tadić, Jovan, Milošević, Snežana, Cingel, Aleksandar, Prodanović, Olivera, Ćosić, Tatjana, Vujčić, Zoran, "Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2" in Scientia Horticulturae, 232 (2018):71-83,
https://doi.org/10.1016/j.scienta.2017.12.024 . .