TY  - CONF
AU  - Belić, Maja
AU  - Tarkowská, Danuše
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5367
AB  - Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5367
ER  - 

@conference{
author = "Belić, Maja and Tarkowská, Danuše and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2022",
abstract = "Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5367"
}

Belić, M., Tarkowská, D., Zdravković-Korać, S.,& Milojević, J.. (2022). Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367

Belić M, Tarkowská D, Zdravković-Korać S, Milojević J. Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .

Belić, Maja, Tarkowská, Danuše, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):26,
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .