TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4318
AB  - Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
T1  - Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4318
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2018",
abstract = "Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts",
title = "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4318"
}

Uzelac, B., Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2018). Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318

Uzelac B, Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts. 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

Uzelac, Branka, Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

TY  - CONF
AU  - Dragićević, Ivana
AU  - Kovačević, Milica
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4320
AB  - Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
T1  - Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4320
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Dragićević, Ivana and Kovačević, Milica and Motyka, Vaclav and Ninković, Slavica and Raspor, Martin",
year = "2018",
abstract = "Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia",
title = "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4320"
}

Uzelac, B., Dragićević, I., Kovačević, M., Motyka, V., Ninković, S.,& Raspor, M.. (2018). Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320

Uzelac B, Dragićević I, Kovačević M, Motyka V, Ninković S, Raspor M. Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia. 2018;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .

Uzelac, Branka, Dragićević, Ivana, Kovačević, Milica, Motyka, Vaclav, Ninković, Slavica, Raspor, Martin, "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro" in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia (2018):26,
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .