TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .