TY  - JOUR
AU  - Karamysheva, Tatyana
AU  - Romanenko, Svetlana
AU  - Makunin, Alexey
AU  - Rajičić, Marija
AU  - Bogdanov, Alexey
AU  - Trifonov, Vladimir
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Orishchenko, Konstantin
AU  - Rubtsov, Nikolay
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/34359988
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8305704
UR  - https://www.mdpi.com/2073-4409/10/7/1819
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4453
AB  - The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.
T2  - Cells
T1  - New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis
IS  - 7
VL  - 10
DO  - 10.3390/cells10071819
SP  - 1819
ER  - 

@article{
author = "Karamysheva, Tatyana and Romanenko, Svetlana and Makunin, Alexey and Rajičić, Marija and Bogdanov, Alexey and Trifonov, Vladimir and Blagojević, Jelena and Vujošević, Mladen and Orishchenko, Konstantin and Rubtsov, Nikolay",
year = "2021",
abstract = "The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.",
journal = "Cells",
title = "New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis",
number = "7",
volume = "10",
doi = "10.3390/cells10071819",
pages = "1819"
}

Karamysheva, T., Romanenko, S., Makunin, A., Rajičić, M., Bogdanov, A., Trifonov, V., Blagojević, J., Vujošević, M., Orishchenko, K.,& Rubtsov, N.. (2021). New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis. in Cells, 10(7), 1819.
https://doi.org/10.3390/cells10071819

Karamysheva T, Romanenko S, Makunin A, Rajičić M, Bogdanov A, Trifonov V, Blagojević J, Vujošević M, Orishchenko K, Rubtsov N. New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis. in Cells. 2021;10(7):1819.
doi:10.3390/cells10071819 .

Karamysheva, Tatyana, Romanenko, Svetlana, Makunin, Alexey, Rajičić, Marija, Bogdanov, Alexey, Trifonov, Vladimir, Blagojević, Jelena, Vujošević, Mladen, Orishchenko, Konstantin, Rubtsov, Nikolay, "New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis" in Cells, 10, no. 7 (2021):1819,
https://doi.org/10.3390/cells10071819 . .