TY  - JOUR
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Blagojević, Duško
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Nestorović, Nataša
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0041008X1730474X
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29217487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2942
AB  - This study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.
T2  - Toxicology and Applied Pharmacology
T2  - Toxicology and Applied Pharmacology
T1  - Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.
VL  - 339
DO  - 10.1016/j.taap.2017.12.001
SP  - 73
EP  - 84
ER  - 

@article{
author = "Jarić, Ivana and Živanović, Jasmina and Miler, Marko and Ajdžanović, Vladimir and Blagojević, Duško and Ristić, Nataša and Milošević, Verica and Nestorović, Nataša",
year = "2018",
abstract = "This study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.",
journal = "Toxicology and Applied Pharmacology, Toxicology and Applied Pharmacology",
title = "Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.",
volume = "339",
doi = "10.1016/j.taap.2017.12.001",
pages = "73-84"
}

Jarić, I., Živanović, J., Miler, M., Ajdžanović, V., Blagojević, D., Ristić, N., Milošević, V.,& Nestorović, N.. (2018). Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.. in Toxicology and Applied Pharmacology, 339, 73-84.
https://doi.org/10.1016/j.taap.2017.12.001

Jarić I, Živanović J, Miler M, Ajdžanović V, Blagojević D, Ristić N, Milošević V, Nestorović N. Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.. in Toxicology and Applied Pharmacology. 2018;339:73-84.
doi:10.1016/j.taap.2017.12.001 .

Jarić, Ivana, Živanović, Jasmina, Miler, Marko, Ajdžanović, Vladimir, Blagojević, Duško, Ristić, Nataša, Milošević, Verica, Nestorović, Nataša, "Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats." in Toxicology and Applied Pharmacology, 339 (2018):73-84,
https://doi.org/10.1016/j.taap.2017.12.001 . .