TY  - CONF
AU  - Horvat, Žolt
AU  - Čabrilo, Borislav
AU  - Paunović, Milan
AU  - Karapandža, Branko
AU  - Josipović, Jelena
AU  - Budinski, Ivana
AU  - Tošić, Božana
AU  - Bjelić Čabrilo, Olivera
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6630
AB  - Bats are the only true flying mammals, and their capacity for flight has contributed to their worldwide distribution. Consequently, they have important functional roles in terrestrial ecosystems in general. These
mammals are reliable bioindicators of the condition of the environments they inhabit and have a regulatory effect on the abundance of crepuscular and nocturnal insects. Many species of bats feed on insects that are either vectors of pathogens that cause diseases of animals and people, or agricultural pests that inflict grave economic losses. The endoparasitic helminths of bats have attracted relatively little attention from parasitologists; therefore, the aim of this study is to contribute to the knowledge on the intestinal Nematodes of Myotis bats in Serbia. We investigated the helminth fauna of 65 individual bats belonging to five different species of the genus Myotis (M. mystacinus, M. alcathoe, M. brandtii, M. blythii, M. myotis). The hosts were sampled from ten sites on the territory of Serbia. Four nematode species were identified: Molinostrongylus alatus, Capillaria neopulchra, Physaloptera sp. and Rictularia bovieri. Intestinal Nematodes infected 48 host individuals, resulting in a prevalence of 73.8%. Molinostrongylus alatus had the highest prevalence (64.6%) and mean abundance (8.2). None of the registered nematode species have zoonotic potential.
PB  - Plovdiv: Medical University of Plovdiv, University Publishing Center
C3  - Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
T1  - The intestinal nematode fauna of bats of the genus Myotis (Chiroptera: Vespertilionidae) in Serbia
SP  - 127
EP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6630
ER  - 

@conference{
author = "Horvat, Žolt and Čabrilo, Borislav and Paunović, Milan and Karapandža, Branko and Josipović, Jelena and Budinski, Ivana and Tošić, Božana and Bjelić Čabrilo, Olivera",
year = "2023",
abstract = "Bats are the only true flying mammals, and their capacity for flight has contributed to their worldwide distribution. Consequently, they have important functional roles in terrestrial ecosystems in general. These
mammals are reliable bioindicators of the condition of the environments they inhabit and have a regulatory effect on the abundance of crepuscular and nocturnal insects. Many species of bats feed on insects that are either vectors of pathogens that cause diseases of animals and people, or agricultural pests that inflict grave economic losses. The endoparasitic helminths of bats have attracted relatively little attention from parasitologists; therefore, the aim of this study is to contribute to the knowledge on the intestinal Nematodes of Myotis bats in Serbia. We investigated the helminth fauna of 65 individual bats belonging to five different species of the genus Myotis (M. mystacinus, M. alcathoe, M. brandtii, M. blythii, M. myotis). The hosts were sampled from ten sites on the territory of Serbia. Four nematode species were identified: Molinostrongylus alatus, Capillaria neopulchra, Physaloptera sp. and Rictularia bovieri. Intestinal Nematodes infected 48 host individuals, resulting in a prevalence of 73.8%. Molinostrongylus alatus had the highest prevalence (64.6%) and mean abundance (8.2). None of the registered nematode species have zoonotic potential.",
publisher = "Plovdiv: Medical University of Plovdiv, University Publishing Center",
journal = "Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria",
title = "The intestinal nematode fauna of bats of the genus Myotis (Chiroptera: Vespertilionidae) in Serbia",
pages = "127-128",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6630"
}

Horvat, Ž., Čabrilo, B., Paunović, M., Karapandža, B., Josipović, J., Budinski, I., Tošić, B.,& Bjelić Čabrilo, O.. (2023). The intestinal nematode fauna of bats of the genus Myotis (Chiroptera: Vespertilionidae) in Serbia. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
Plovdiv: Medical University of Plovdiv, University Publishing Center., 127-128.
https://hdl.handle.net/21.15107/rcub_ibiss_6630

Horvat Ž, Čabrilo B, Paunović M, Karapandža B, Josipović J, Budinski I, Tošić B, Bjelić Čabrilo O. The intestinal nematode fauna of bats of the genus Myotis (Chiroptera: Vespertilionidae) in Serbia. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria. 2023;:127-128.
https://hdl.handle.net/21.15107/rcub_ibiss_6630 .

Horvat, Žolt, Čabrilo, Borislav, Paunović, Milan, Karapandža, Branko, Josipović, Jelena, Budinski, Ivana, Tošić, Božana, Bjelić Čabrilo, Olivera, "The intestinal nematode fauna of bats of the genus Myotis (Chiroptera: Vespertilionidae) in Serbia" in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria (2023):127-128,
https://hdl.handle.net/21.15107/rcub_ibiss_6630 .

TY  - CONF
AU  - Tošić, Božana
AU  - Čabrilo, Borislav
AU  - Miljević, Milan
AU  - Blagojević, Jelena
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Bjelić Čabrilo, Olivera
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6509
AB  - As part of research activities focused on rodent helminth fauna in Serbia, over the course of the previous decade, we registered the pres-ence of species with zoonotic potential, Moniliformis moniliformis, an acan-thocephalan (or spiny-headed worm) intestinal parasite that primarily in-fects rats, mice and voles. The adult form of the species M. moniliformis was found in the small intestine of three individuals of the yellow-necked mouse (Apodemus flavicollis) in the localities of Donji Milanovac, Cer Moun-tain and Senokos. Another species of Acanthocephala, reported in earlier studies of Spermophilus citellus in Serbia, is Macracanthorhynchus hirudi-naceus, which also has zoonotic potential. Data on human infection with the aforementioned acanthocephalans are scarce all over the world, in-cluding Serbia, but they should not be ignored. Considering that the pres-ence of spiny-headed worms with zoonotic potential in our country was established during the last years of research, it is necessary to develop awareness of the potential risk for public health and actively monitor this group of helminths and their definitive hosts throughout the country. Reg-ular deratisation and disinsection in order to regulate the number of pop-ulations of rodents and insects that transmit acanthocephalans among other helminths in urban areas, are of great importance for the prevention of infection with these helminths. Implementing basic hygiene measures, as well as maintaining a healthy environment are essential measures to prevent the transmission of infection to humans. These proposed activities are in line with the One health approach, which stands at the intersection of human, animal and environmental health.
PB  - Plovdiv: Medical University of Plovdiv, University Publishing Center
C3  - Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
T1  - Acanthocephalans in Serbia: rare, but not unimportant
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6509
ER  - 

@conference{
author = "Tošić, Božana and Čabrilo, Borislav and Miljević, Milan and Blagojević, Jelena and Bajić, Branka and Rajičić, Marija and Budinski, Ivana and Bjelić Čabrilo, Olivera",
year = "2023",
abstract = "As part of research activities focused on rodent helminth fauna in Serbia, over the course of the previous decade, we registered the pres-ence of species with zoonotic potential, Moniliformis moniliformis, an acan-thocephalan (or spiny-headed worm) intestinal parasite that primarily in-fects rats, mice and voles. The adult form of the species M. moniliformis was found in the small intestine of three individuals of the yellow-necked mouse (Apodemus flavicollis) in the localities of Donji Milanovac, Cer Moun-tain and Senokos. Another species of Acanthocephala, reported in earlier studies of Spermophilus citellus in Serbia, is Macracanthorhynchus hirudi-naceus, which also has zoonotic potential. Data on human infection with the aforementioned acanthocephalans are scarce all over the world, in-cluding Serbia, but they should not be ignored. Considering that the pres-ence of spiny-headed worms with zoonotic potential in our country was established during the last years of research, it is necessary to develop awareness of the potential risk for public health and actively monitor this group of helminths and their definitive hosts throughout the country. Reg-ular deratisation and disinsection in order to regulate the number of pop-ulations of rodents and insects that transmit acanthocephalans among other helminths in urban areas, are of great importance for the prevention of infection with these helminths. Implementing basic hygiene measures, as well as maintaining a healthy environment are essential measures to prevent the transmission of infection to humans. These proposed activities are in line with the One health approach, which stands at the intersection of human, animal and environmental health.",
publisher = "Plovdiv: Medical University of Plovdiv, University Publishing Center",
journal = "Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria",
title = "Acanthocephalans in Serbia: rare, but not unimportant",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6509"
}

Tošić, B., Čabrilo, B., Miljević, M., Blagojević, J., Bajić, B., Rajičić, M., Budinski, I.,& Bjelić Čabrilo, O.. (2023). Acanthocephalans in Serbia: rare, but not unimportant. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
Plovdiv: Medical University of Plovdiv, University Publishing Center., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_6509

Tošić B, Čabrilo B, Miljević M, Blagojević J, Bajić B, Rajičić M, Budinski I, Bjelić Čabrilo O. Acanthocephalans in Serbia: rare, but not unimportant. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria. 2023;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_6509 .

Tošić, Božana, Čabrilo, Borislav, Miljević, Milan, Blagojević, Jelena, Bajić, Branka, Rajičić, Marija, Budinski, Ivana, Bjelić Čabrilo, Olivera, "Acanthocephalans in Serbia: rare, but not unimportant" in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria (2023):65,
https://hdl.handle.net/21.15107/rcub_ibiss_6509 .

TY  - CONF
AU  - Tošić, Božana
AU  - Čabrilo, Borislav
AU  - Miljević, Milan
AU  - Bjelić Čabrilo, Olivera
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6506
AB  - Heligmosomoides nematodes are frequent intestinal parasites of rodents, primarily mice and voles. The common species H. polygyrus is closely related to the highly prevalent nematodes infecting humans. It is assumed that the Asian species H. neopolygyrus arrived in Europe with its host Apodemus agrarius, and the presence of this nematode was first rec-orded in a striped field mouse in Poland in 2014. We present a preliminary report of two A. agrarius from the Special Nature Reserve Koviljsko-petrova-radinski rit and Kameničko Island in Serbia, which were found to be in-fected with H. neopolygyrus. Current research is based on phylogenetic analyses of cyt b sequences of Heligmosomoides isolated from Apodemus species. Preliminary results showed clustering of sequences isolates from A.agrarius from this study with those of H. neopolygyrus from A. agrariusfrom Poland, but also with H. polygyrus from A. agrarius (Poland) and A. ura-lensis (Russia). Due to the great morphological similarities of H. polygyrusand H. neopolygyrus, some specimens may have previously been misiden-tified as H. polygyrus, which is why the presence of H. neopolygyrus on theEuropean continent was only recently established. The most significantmorphological difference between the two species is seen in the proximalsection of external dorsal rays of the male's copulatory bursa, which is fili-form in H. neopolygyrus and swollen in H. polygyrus. The use of molecularmarkers may overcome the problems of morphological misidentificationin the future. Considering the biology and ecology of other Apodemus spe-cies, as well as voles, the presence of H. neopolygyrus can be expected inthese hosts as well.
PB  - Plovdiv: Medical University of Plovdiv, University Publishing Center
C3  - Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
T1  - First record of Heligmosomoides neopolygyrus in Serbia
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6506
ER  - 

@conference{
author = "Tošić, Božana and Čabrilo, Borislav and Miljević, Milan and Bjelić Čabrilo, Olivera and Bajić, Branka and Rajičić, Marija and Budinski, Ivana and Blagojević, Jelena",
year = "2023",
abstract = "Heligmosomoides nematodes are frequent intestinal parasites of rodents, primarily mice and voles. The common species H. polygyrus is closely related to the highly prevalent nematodes infecting humans. It is assumed that the Asian species H. neopolygyrus arrived in Europe with its host Apodemus agrarius, and the presence of this nematode was first rec-orded in a striped field mouse in Poland in 2014. We present a preliminary report of two A. agrarius from the Special Nature Reserve Koviljsko-petrova-radinski rit and Kameničko Island in Serbia, which were found to be in-fected with H. neopolygyrus. Current research is based on phylogenetic analyses of cyt b sequences of Heligmosomoides isolated from Apodemus species. Preliminary results showed clustering of sequences isolates from A.agrarius from this study with those of H. neopolygyrus from A. agrariusfrom Poland, but also with H. polygyrus from A. agrarius (Poland) and A. ura-lensis (Russia). Due to the great morphological similarities of H. polygyrusand H. neopolygyrus, some specimens may have previously been misiden-tified as H. polygyrus, which is why the presence of H. neopolygyrus on theEuropean continent was only recently established. The most significantmorphological difference between the two species is seen in the proximalsection of external dorsal rays of the male's copulatory bursa, which is fili-form in H. neopolygyrus and swollen in H. polygyrus. The use of molecularmarkers may overcome the problems of morphological misidentificationin the future. Considering the biology and ecology of other Apodemus spe-cies, as well as voles, the presence of H. neopolygyrus can be expected inthese hosts as well.",
publisher = "Plovdiv: Medical University of Plovdiv, University Publishing Center",
journal = "Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria",
title = "First record of Heligmosomoides neopolygyrus in Serbia",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6506"
}

Tošić, B., Čabrilo, B., Miljević, M., Bjelić Čabrilo, O., Bajić, B., Rajičić, M., Budinski, I.,& Blagojević, J.. (2023). First record of Heligmosomoides neopolygyrus in Serbia. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria
Plovdiv: Medical University of Plovdiv, University Publishing Center., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_6506

Tošić B, Čabrilo B, Miljević M, Bjelić Čabrilo O, Bajić B, Rajičić M, Budinski I, Blagojević J. First record of Heligmosomoides neopolygyrus in Serbia. in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria. 2023;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_6506 .

Tošić, Božana, Čabrilo, Borislav, Miljević, Milan, Bjelić Čabrilo, Olivera, Bajić, Branka, Rajičić, Marija, Budinski, Ivana, Blagojević, Jelena, "First record of Heligmosomoides neopolygyrus in Serbia" in Program and Abstract: International Conference One Health and Zoology; 2023 Sep 27–29; Hissarya, Bulgaria (2023):126,
https://hdl.handle.net/21.15107/rcub_ibiss_6506 .

TY  - JOUR
AU  - Jojić, Vida
AU  - Bajić, Aleksandar
AU  - Barišić Klisarić, Nataša
AU  - Bugarski-Stanojević, Vanja
AU  - Snoj, Aleš
AU  - Miljanović, Branko
AU  - Askeyev, Oleg
AU  - Askeyev, Igor
AU  - Marić, Saša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6390
AB  - This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.
PB  - Leiden: Brill
T2  - Contributions to Zoology
T1  - Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)
IS  - 5
VL  - 92
DO  - 10.1163/18759866-bja10051
SP  - 510
EP  - 532
ER  - 

@article{
author = "Jojić, Vida and Bajić, Aleksandar and Barišić Klisarić, Nataša and Bugarski-Stanojević, Vanja and Snoj, Aleš and Miljanović, Branko and Askeyev, Oleg and Askeyev, Igor and Marić, Saša",
year = "2023",
abstract = "This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.",
publisher = "Leiden: Brill",
journal = "Contributions to Zoology",
title = "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)",
number = "5",
volume = "92",
doi = "10.1163/18759866-bja10051",
pages = "510-532"
}

Jojić, V., Bajić, A., Barišić Klisarić, N., Bugarski-Stanojević, V., Snoj, A., Miljanović, B., Askeyev, O., Askeyev, I.,& Marić, S.. (2023). Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology
Leiden: Brill., 92(5), 510-532.
https://doi.org/10.1163/18759866-bja10051

Jojić V, Bajić A, Barišić Klisarić N, Bugarski-Stanojević V, Snoj A, Miljanović B, Askeyev O, Askeyev I, Marić S. Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology. 2023;92(5):510-532.
doi:10.1163/18759866-bja10051 .

Jojić, Vida, Bajić, Aleksandar, Barišić Klisarić, Nataša, Bugarski-Stanojević, Vanja, Snoj, Aleš, Miljanović, Branko, Askeyev, Oleg, Askeyev, Igor, Marić, Saša, "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)" in Contributions to Zoology, 92, no. 5 (2023):510-532,
https://doi.org/10.1163/18759866-bja10051 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.hbd-sbc.hr/kongres2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6564
AB  - Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
PB  - Zagreb: Hrvatsko biološko društvo
C3  - Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
T1  - Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
SP  - 231
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6564
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Karan, Jelena and Ivelja, Ivana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.",
publisher = "Zagreb: Hrvatsko biološko društvo",
journal = "Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska",
title = "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes",
pages = "231-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6564"
}

Marković Filipović, J., Kojić, D., Miler, M., Karan, J., Ivelja, I.,& Matavulj, M.. (2022). Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
Zagreb: Hrvatsko biološko društvo., 231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564

Marković Filipović J, Kojić D, Miler M, Karan J, Ivelja I, Matavulj M. Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. 2022;:231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Karan, Jelena, Ivelja, Ivana, Matavulj, Milica, "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes" in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska (2022):231-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

TY  - CONF
AU  - Bjelić Čabrilo, Olivera
AU  - Blagojević, Jelena
AU  - Čabrilo, Borislav
AU  - Miljević, Milan
AU  - Tošić, Božana
AU  - Savić, Sara
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6514
AB  - Iako je odavno poznato da postoji povezanost između zdravlja životne sredine, životinja i ljudi, svest o ovom konceptu je podignuta relativno nedavno. Koliko je ova povezanost jaka jasno se može uočiti kada antropogeni uticaj izvede prirodne ekosisteme iz stanja ravnoteže. Tokom proteklih decenija putovanja su sve češća, te čovek može preći velike razdaljine za kratko vreme. Jedna osoba ili kućni ljubimac koji putuje sa vlasnikom može preneti zaraznu bolest sa jednog kraja sveta na drugi za 24 časa, što može dovesti do velikih zdravstvenih problema, pa i epidemija u novoj sredini. U takvim slučajevima, intervencije i njihovi rezultati zavise od saradnje učesnika u održavanju javnog zdravlja ljudi, zdravlja životinja i životne sredine. Značajno mesto u tom procesu, pored medicinara i veterinara, imaju i biolozi i ekolozi, koji su eksperti za praćenje stanja ekosistema i kretanja populacija divljih vrsta životinja koje mogu biti vektori, prelazni, paratenični i definitivni domaćini patogena. Oni utvrđuju stanje sredine, stepen izmenjenosti pod antropogenim uticajem i nivo poremećaja brojčanih odnosa populacija u zajednici. Takođe, mogu utvrditi prisustvo patogena u divljim populacijama, što je naročito značajno tamo gde je moguć njihov kontakt sa ljudima i domaćim životinjama. Ovde će biti prikazan kratak pregled jednog broja takvih studija, čiji značaj je izuzetan, jer prisustvo patogena kod divljih životinja ukazuje na njegovo prisustvo u okruženju, odakle se lako prenosi na ljude
AB  - Иако је одавно познато да постоји повезаност између здравља животне средине,
животиња и људи, свест о овом концепту је подигнутa релативно недавно.1 Колико
је ова повезаност јака јасно се може уочити када антропогени утицај изведе
природне екосистеме из стања равнотеже. Током протеклих деценија путовања су
све чешћа, те човек може прећи велике раздаљине за кратко време. Једна особа или
кућни љубимац који путује са власником може пренети заразну болест са једног
краја света на други за 24 часа, што може довести до великих здравствених
проблема, па и епидемија у новој средини. У таквим случајевима, интервенције и
њихови резултати зависе од сарадње учесника у одржавању јавног здравља људи,
здравља животиња и животне средине. Значајно место у том процесу, поред
медицинара и ветеринара, имају и биолози и еколози, који су експерти за праћење
стања екосистема и кретања популација дивљих врста животиња које могу бити
вектори, прелазни, паратенични и дефинитивни домаћини патогена. Они утврђују
стање средине, степен измењености под антропогеним утицајем и ниво поремећаја
бројчаних односа популација у заједници. Такође, могу утврдити присуство
патогена у дивљим популацијама, што је нарочито значајно тамо где је могућ
њихов контакт са људима и домаћим животињама.1,2,3 Овде ће бити приказан
кратак преглед једног броја таквих студија, чији значај је изузетан, јер присуство
патогена код дивљих животиња указује на његово присуство у окружењу, одакле
се лако преноси на људе.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Zdrava životna sredina-zdravlje životinja-zdravlje ljudi: značaj koncepta Jedno zdravlje
T1  - Здрава животна средина-здравље животиња-здравље људи: значај концепта Једно здравље
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6514
ER  - 

@conference{
author = "Bjelić Čabrilo, Olivera and Blagojević, Jelena and Čabrilo, Borislav and Miljević, Milan and Tošić, Božana and Savić, Sara",
year = "2022",
abstract = "Iako je odavno poznato da postoji povezanost između zdravlja životne sredine, životinja i ljudi, svest o ovom konceptu je podignuta relativno nedavno. Koliko je ova povezanost jaka jasno se može uočiti kada antropogeni uticaj izvede prirodne ekosisteme iz stanja ravnoteže. Tokom proteklih decenija putovanja su sve češća, te čovek može preći velike razdaljine za kratko vreme. Jedna osoba ili kućni ljubimac koji putuje sa vlasnikom može preneti zaraznu bolest sa jednog kraja sveta na drugi za 24 časa, što može dovesti do velikih zdravstvenih problema, pa i epidemija u novoj sredini. U takvim slučajevima, intervencije i njihovi rezultati zavise od saradnje učesnika u održavanju javnog zdravlja ljudi, zdravlja životinja i životne sredine. Značajno mesto u tom procesu, pored medicinara i veterinara, imaju i biolozi i ekolozi, koji su eksperti za praćenje stanja ekosistema i kretanja populacija divljih vrsta životinja koje mogu biti vektori, prelazni, paratenični i definitivni domaćini patogena. Oni utvrđuju stanje sredine, stepen izmenjenosti pod antropogenim uticajem i nivo poremećaja brojčanih odnosa populacija u zajednici. Takođe, mogu utvrditi prisustvo patogena u divljim populacijama, što je naročito značajno tamo gde je moguć njihov kontakt sa ljudima i domaćim životinjama. Ovde će biti prikazan kratak pregled jednog broja takvih studija, čiji značaj je izuzetan, jer prisustvo patogena kod divljih životinja ukazuje na njegovo prisustvo u okruženju, odakle se lako prenosi na ljude, Иако је одавно познато да постоји повезаност између здравља животне средине,
животиња и људи, свест о овом концепту је подигнутa релативно недавно.1 Колико
је ова повезаност јака јасно се може уочити када антропогени утицај изведе
природне екосистеме из стања равнотеже. Током протеклих деценија путовања су
све чешћа, те човек може прећи велике раздаљине за кратко време. Једна особа или
кућни љубимац који путује са власником може пренети заразну болест са једног
краја света на други за 24 часа, што може довести до великих здравствених
проблема, па и епидемија у новој средини. У таквим случајевима, интервенције и
њихови резултати зависе од сарадње учесника у одржавању јавног здравља људи,
здравља животиња и животне средине. Значајно место у том процесу, поред
медицинара и ветеринара, имају и биолози и еколози, који су експерти за праћење
стања екосистема и кретања популација дивљих врста животиња које могу бити
вектори, прелазни, паратенични и дефинитивни домаћини патогена. Они утврђују
стање средине, степен измењености под антропогеним утицајем и ниво поремећаја
бројчаних односа популација у заједници. Такође, могу утврдити присуство
патогена у дивљим популацијама, што је нарочито значајно тамо где је могућ
њихов контакт са људима и домаћим животињама.1,2,3 Овде ће бити приказан
кратак преглед једног броја таквих студија, чији значај је изузетан, јер присуство
патогена код дивљих животиња указује на његово присуство у окружењу, одакле
се лако преноси на људе.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Zdrava životna sredina-zdravlje životinja-zdravlje ljudi: značaj koncepta Jedno zdravlje, Здрава животна средина-здравље животиња-здравље људи: значај концепта Једно здравље",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6514"
}

Bjelić Čabrilo, O., Blagojević, J., Čabrilo, B., Miljević, M., Tošić, B.,& Savić, S.. (2022). Zdrava životna sredina-zdravlje životinja-zdravlje ljudi: značaj koncepta Jedno zdravlje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_6514

Bjelić Čabrilo O, Blagojević J, Čabrilo B, Miljević M, Tošić B, Savić S. Zdrava životna sredina-zdravlje životinja-zdravlje ljudi: značaj koncepta Jedno zdravlje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_6514 .

Bjelić Čabrilo, Olivera, Blagojević, Jelena, Čabrilo, Borislav, Miljević, Milan, Tošić, Božana, Savić, Sara, "Zdrava životna sredina-zdravlje životinja-zdravlje ljudi: značaj koncepta Jedno zdravlje" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):129,
https://hdl.handle.net/21.15107/rcub_ibiss_6514 .

TY  - JOUR
AU  - Marković-Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Andrejić Višnjić, Bojana
AU  - Milošević, Verica
AU  - Cukuranovic Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Matavulj, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5480
AB  - Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.
T2  - International Journal of Morphology
T1  - Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study
IS  - 6
VL  - 40
DO  - 10.4067/s0717-95022022000601618
SP  - 1618
EP  - 1623
ER  - 

@article{
author = "Marković-Filipović, Jelena and Miler, Marko and Kojić, Danijela and Andrejić Višnjić, Bojana and Milošević, Verica and Cukuranovic Kokoris, Jovana and Đorđević, Miodrag and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.",
journal = "International Journal of Morphology",
title = "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study",
number = "6",
volume = "40",
doi = "10.4067/s0717-95022022000601618",
pages = "1618-1623"
}

Marković-Filipović, J., Miler, M., Kojić, D., Andrejić Višnjić, B., Milošević, V., Cukuranovic Kokoris, J., Đorđević, M.,& Matavulj, M.. (2022). Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology, 40(6), 1618-1623.
https://doi.org/10.4067/s0717-95022022000601618

Marković-Filipović J, Miler M, Kojić D, Andrejić Višnjić B, Milošević V, Cukuranovic Kokoris J, Đorđević M, Matavulj M. Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology. 2022;40(6):1618-1623.
doi:10.4067/s0717-95022022000601618 .

Marković-Filipović, Jelena, Miler, Marko, Kojić, Danijela, Andrejić Višnjić, Bojana, Milošević, Verica, Cukuranovic Kokoris, Jovana, Đorđević, Miodrag, Matavulj, Milica, "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study" in International Journal of Morphology, 40, no. 6 (2022):1618-1623,
https://doi.org/10.4067/s0717-95022022000601618 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6036
AB  - Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova.
AB  - Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova
T1  - Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова
SP  - 366
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6036
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Matavulj, Milica",
year = "2022",
abstract = "Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova., Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova, Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова",
pages = "366-366",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6036"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M.,& Matavulj, M.. (2022). Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036

Marković Filipović J, Ivelja I, Karan J, Miler M, Matavulj M. Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Matavulj, Milica, "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):366-366,
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

TY  - CONF
AU  - Bjelić Čabrilo, Olivera
AU  - Čabrilo, Borislav
AU  - Miljević, Milan
AU  - Tošić, Božana
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5193
AB  - Helminths represent hidden components of biodiversity in natural communities. Rather than elicit interest in their conservation, these endoparasitic worms tend to cause indifference or even disgust because of their appearance and way of life. However, they are present in every ecosystem and play a key role by integrating themselves into food webs and thereby securing their survival. Over the course of decades of research, 47 helminths were recorded in 10 rodent species in Serbia, with nematodes being the dominant group (29 species). Some helminths have high zoonotic potential due to their ability to infect humans, pets and domestic animals. This study focusses on hepatic helminths in a sample of six host species analysed over an eight-year period. Three tapeworm species and one nematode species were recorded, and their significance is broadly stated.
A total of 770 wild rodents belonging to the species Apodemus flavicollis, A. agrarius, A. sylvaticus, Myodes glareolus, Microtus arvalis, and M. subterraneus were captured from 42 sites in Serbia from 2013-2021, dissected, and examined for cysts and lesions. For confirmation of parasite species, DNA was extracted and mitochondrial marker 12S rDNA was amplified and sequenced. Nematode eggs and larvae were detected based on histological slides made from the liver samples. 
The livers of 47 hosts contained cysts or visible lesions (6.1%). 12S rDNA fragment amplified successfully in 13 larval samples (1.68%), and three Taenia species were identified. Hydatigera (Taenia) taeniaeformis was dominant, followed by T. martis and T. crassiceps. No cysts were found in A. sylvaticus and M. subterraneus. Histological examination of the liver confirmed the presence of the nematode Calodium hepaticum in all three Apodemus host species, with a prevalence of 0.9%.
Carnivores act as definitive hosts for all the registered tapeworm species: canids for T. crassiceps, felids for T. taeniaeformis (including domestic dogs and cats) and mustelids for T. martis. Rodents are intermediate hosts and carry cysts (cysticerci) on their livers. All three species have been confirmed to infect humans. Another tapeworm species whose larvae are found in rodent livers is Echinococcus multilocularis. This parasite has been found in foxes and jackals in Serbia, and it is only a matter of time before it is reported from rodents as well. Considering the grave clinical presentation of E. multilocularis infection in humans, increased vigilance and monitoring of its presence in intermediate and definitive hosts is advised. Both adults and eggs of C. hepaticum are found in rodent hepatic tissue but can infect other animals and humans. Infections are most frequently reported in children aged one to five and may be fatal.
While far from attractive in the most general sense, helminths are an important component of biodiversity in every ecosystem and affect the behaviour, fitness and abundance of its constituent species. They can indicate the condition and population dynamics of endangered species and overall ecosystem function. Due to their effects on humans and domestic animals, it is of key importance to monitor helminth diversity and populations in intermediate and definitive hosts.
PB  - Skopje: Macedonian Ecological Society
C3  - Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
T1  - Rodent hepatic helminths as components of hidden biodiversity
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5193
ER  - 

@conference{
author = "Bjelić Čabrilo, Olivera and Čabrilo, Borislav and Miljević, Milan and Tošić, Božana and Bajić, Branka and Rajičić, Marija and Budinski, Ivana and Blagojević, Jelena",
year = "2022",
abstract = "Helminths represent hidden components of biodiversity in natural communities. Rather than elicit interest in their conservation, these endoparasitic worms tend to cause indifference or even disgust because of their appearance and way of life. However, they are present in every ecosystem and play a key role by integrating themselves into food webs and thereby securing their survival. Over the course of decades of research, 47 helminths were recorded in 10 rodent species in Serbia, with nematodes being the dominant group (29 species). Some helminths have high zoonotic potential due to their ability to infect humans, pets and domestic animals. This study focusses on hepatic helminths in a sample of six host species analysed over an eight-year period. Three tapeworm species and one nematode species were recorded, and their significance is broadly stated.
A total of 770 wild rodents belonging to the species Apodemus flavicollis, A. agrarius, A. sylvaticus, Myodes glareolus, Microtus arvalis, and M. subterraneus were captured from 42 sites in Serbia from 2013-2021, dissected, and examined for cysts and lesions. For confirmation of parasite species, DNA was extracted and mitochondrial marker 12S rDNA was amplified and sequenced. Nematode eggs and larvae were detected based on histological slides made from the liver samples. 
The livers of 47 hosts contained cysts or visible lesions (6.1%). 12S rDNA fragment amplified successfully in 13 larval samples (1.68%), and three Taenia species were identified. Hydatigera (Taenia) taeniaeformis was dominant, followed by T. martis and T. crassiceps. No cysts were found in A. sylvaticus and M. subterraneus. Histological examination of the liver confirmed the presence of the nematode Calodium hepaticum in all three Apodemus host species, with a prevalence of 0.9%.
Carnivores act as definitive hosts for all the registered tapeworm species: canids for T. crassiceps, felids for T. taeniaeformis (including domestic dogs and cats) and mustelids for T. martis. Rodents are intermediate hosts and carry cysts (cysticerci) on their livers. All three species have been confirmed to infect humans. Another tapeworm species whose larvae are found in rodent livers is Echinococcus multilocularis. This parasite has been found in foxes and jackals in Serbia, and it is only a matter of time before it is reported from rodents as well. Considering the grave clinical presentation of E. multilocularis infection in humans, increased vigilance and monitoring of its presence in intermediate and definitive hosts is advised. Both adults and eggs of C. hepaticum are found in rodent hepatic tissue but can infect other animals and humans. Infections are most frequently reported in children aged one to five and may be fatal.
While far from attractive in the most general sense, helminths are an important component of biodiversity in every ecosystem and affect the behaviour, fitness and abundance of its constituent species. They can indicate the condition and population dynamics of endangered species and overall ecosystem function. Due to their effects on humans and domestic animals, it is of key importance to monitor helminth diversity and populations in intermediate and definitive hosts.",
publisher = "Skopje: Macedonian Ecological Society",
journal = "Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia",
title = "Rodent hepatic helminths as components of hidden biodiversity",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5193"
}

Bjelić Čabrilo, O., Čabrilo, B., Miljević, M., Tošić, B., Bajić, B., Rajičić, M., Budinski, I.,& Blagojević, J.. (2022). Rodent hepatic helminths as components of hidden biodiversity. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
Skopje: Macedonian Ecological Society., 50.
https://hdl.handle.net/21.15107/rcub_ibiss_5193

Bjelić Čabrilo O, Čabrilo B, Miljević M, Tošić B, Bajić B, Rajičić M, Budinski I, Blagojević J. Rodent hepatic helminths as components of hidden biodiversity. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia. 2022;:50.
https://hdl.handle.net/21.15107/rcub_ibiss_5193 .

Bjelić Čabrilo, Olivera, Čabrilo, Borislav, Miljević, Milan, Tošić, Božana, Bajić, Branka, Rajičić, Marija, Budinski, Ivana, Blagojević, Jelena, "Rodent hepatic helminths as components of hidden biodiversity" in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia (2022):50,
https://hdl.handle.net/21.15107/rcub_ibiss_5193 .

TY  - CONF
AU  - Čabrilo, Borislav
AU  - Tošić, Božana
AU  - Miljević, Milan
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Bajić, Branka
AU  - Bjelić Čabrilo, Olivera
AU  - Blagojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5192
AB  - Endoparasites are organisms that live in a very specific type of habitat: the internal organs of their hosts. The effect of host characteristics on parasites and vice versa can thus be compared to the interplay between free-living organisms and their environment. Host-parasite interactions are astoundingly complex and many studies to this date have attempted to shed more light on them. In the current study, we investigated the relationship between selected host traits and intestinal nematode abundance. The intestinal tracts of 76 rodents of seven species (Apodemus agrarius, A. flavicollis, A. sylvaticus, Micromys minutus, Microtus arvalis, M. agrestis, Mus musculus) from 6 localities in the Vojvodina province of Serbia were searched for nematode parasites. Biometric parameters (body mass, body, tail, hindfoot and ear length) and spleen mass were measured for all hosts. The intestinal tract of the dissected rodents was cut open and its contents examined under stereomicroscope; nematodes were collected and stored in 70% alcohol prior to species identification. The most common nematode species in the host sample was the trichostrongyloid Heligmosomoides polygyrus. To test for possible cause-and-effect relationships, linear regression was carried out with spleen mass as the response variable and body mass, body, tail, hindfoot and ear length, number of H. polygyrus and total number of nematodes as predictor variables. A separate linear regression used H. polygyrus and total nematode abundance as response variables, and host body mass and length as predictor variables. Body mass (F = 21.259, p < 0.001), body length (F = 18.208, p < 0.001), tail length (F = 4.465, p = 0.038), ear length (F = 5.181, p = 0.026) and hindfoot length (F = 5.089, p = 0.027) all significantly and positively influenced spleen mass. Conversely, abundance of H. polygyrus and intestinal nematodes in general had no significant effect on spleen mass. However, body mass (F = 23.173, p < 0.001) and body length (F = 9.746, p = 0.003) of the host significantly and positively affected H. polygyrus abundance; neither factor influenced total nematode abundance. The regression results suggest that there is a positive cause-and-effect relationship between host body size (mass and length) and the number of H. polygyrus worms. Such a result provides support for the hypothesis that larger hosts should be more infected because they represent better targets for parasites. While practically invisible, parasites are an important component of every community on planet Earth. With this in mind, disentangling the complicated biotic interactions between host characteristics and parasite abundance is an important part of ecological studies, as it provides us with a better understanding of processes and relationships within any given ecosystem.
PB  - Skopje: Macedonian Ecological Society
C3  - Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
T1  - Relationship between host traits and intestinal nematode abundance in rodents of Vojvodine province, Serbia
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5192
ER  - 

@conference{
author = "Čabrilo, Borislav and Tošić, Božana and Miljević, Milan and Budinski, Ivana and Rajičić, Marija and Bajić, Branka and Bjelić Čabrilo, Olivera and Blagojević, Jelena",
year = "2022",
abstract = "Endoparasites are organisms that live in a very specific type of habitat: the internal organs of their hosts. The effect of host characteristics on parasites and vice versa can thus be compared to the interplay between free-living organisms and their environment. Host-parasite interactions are astoundingly complex and many studies to this date have attempted to shed more light on them. In the current study, we investigated the relationship between selected host traits and intestinal nematode abundance. The intestinal tracts of 76 rodents of seven species (Apodemus agrarius, A. flavicollis, A. sylvaticus, Micromys minutus, Microtus arvalis, M. agrestis, Mus musculus) from 6 localities in the Vojvodina province of Serbia were searched for nematode parasites. Biometric parameters (body mass, body, tail, hindfoot and ear length) and spleen mass were measured for all hosts. The intestinal tract of the dissected rodents was cut open and its contents examined under stereomicroscope; nematodes were collected and stored in 70% alcohol prior to species identification. The most common nematode species in the host sample was the trichostrongyloid Heligmosomoides polygyrus. To test for possible cause-and-effect relationships, linear regression was carried out with spleen mass as the response variable and body mass, body, tail, hindfoot and ear length, number of H. polygyrus and total number of nematodes as predictor variables. A separate linear regression used H. polygyrus and total nematode abundance as response variables, and host body mass and length as predictor variables. Body mass (F = 21.259, p < 0.001), body length (F = 18.208, p < 0.001), tail length (F = 4.465, p = 0.038), ear length (F = 5.181, p = 0.026) and hindfoot length (F = 5.089, p = 0.027) all significantly and positively influenced spleen mass. Conversely, abundance of H. polygyrus and intestinal nematodes in general had no significant effect on spleen mass. However, body mass (F = 23.173, p < 0.001) and body length (F = 9.746, p = 0.003) of the host significantly and positively affected H. polygyrus abundance; neither factor influenced total nematode abundance. The regression results suggest that there is a positive cause-and-effect relationship between host body size (mass and length) and the number of H. polygyrus worms. Such a result provides support for the hypothesis that larger hosts should be more infected because they represent better targets for parasites. While practically invisible, parasites are an important component of every community on planet Earth. With this in mind, disentangling the complicated biotic interactions between host characteristics and parasite abundance is an important part of ecological studies, as it provides us with a better understanding of processes and relationships within any given ecosystem.",
publisher = "Skopje: Macedonian Ecological Society",
journal = "Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia",
title = "Relationship between host traits and intestinal nematode abundance in rodents of Vojvodine province, Serbia",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5192"
}

Čabrilo, B., Tošić, B., Miljević, M., Budinski, I., Rajičić, M., Bajić, B., Bjelić Čabrilo, O.,& Blagojević, J.. (2022). Relationship between host traits and intestinal nematode abundance in rodents of Vojvodine province, Serbia. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
Skopje: Macedonian Ecological Society., 164.
https://hdl.handle.net/21.15107/rcub_ibiss_5192

Čabrilo B, Tošić B, Miljević M, Budinski I, Rajičić M, Bajić B, Bjelić Čabrilo O, Blagojević J. Relationship between host traits and intestinal nematode abundance in rodents of Vojvodine province, Serbia. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia. 2022;:164.
https://hdl.handle.net/21.15107/rcub_ibiss_5192 .

Čabrilo, Borislav, Tošić, Božana, Miljević, Milan, Budinski, Ivana, Rajičić, Marija, Bajić, Branka, Bjelić Čabrilo, Olivera, Blagojević, Jelena, "Relationship between host traits and intestinal nematode abundance in rodents of Vojvodine province, Serbia" in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia (2022):164,
https://hdl.handle.net/21.15107/rcub_ibiss_5192 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6577
AB  - INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver
SP  - 137
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6577
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver",
pages = "137-138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6577"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577

Marković Filipović J, Ivelja I, Karan J, Miler M, Milošević V, Matavulj M. Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):137-138,
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6578
AB  - INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Acrylamide treatment affects oxidative stress parameters in rat hepatocytes
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6578
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6578"
}

Marković Filipović, J., Kojić, D., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578

Marković Filipović J, Kojić D, Miler M, Milošević V, Matavulj M. Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

TY  - JOUR
AU  - Abramović, Biljana F.
AU  - Uzelac, Maria M.
AU  - Armaković, Sanja J.
AU  - Gašić, Uroš
AU  - Četojević-Simin, Dragana D.
AU  - Armaković, Stevan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4205
AB  - In this work, we have experimentally and computationally investigated the process of hydrolysis and photolysis of cephalosporin antibiotics with ceftriaxone (CEF) as a model compound. The CEF hydrolysis was investigated in ultrapure and natural water, at 25 ± 1 °C and 4 ± 1 °C in the dark. It was found that CEF after 100 and 900 days at 25 ± 1°C and 4 ± 1 °C, respectively practically completely removed from ultrapure water. The CEF hydrolysis in natural water was five and three times slower at 25 ± 1 °C and 4 ± 1 °C, respectively than in ultrapure water. Further, the efficiency of direct photolysis (solar/UVA-B) and solar/H2O2 treatment of CEF was investigated. Under UVA-B radiation 95.6% of CEF was removed after 60 min, while for the same time of solar radiation degradation was practically not observed (only 3.2%). Also, the effects of different concentrations of H2O2 (0–150 mM) in the presence/absence of solar radiation were studied. The most efficient solar/H2O2 treatment was in the presence of 90 mM H2O2, whereby 66.8% of CEF was removed after 60 min (41.8% by indirect photolysis, 21.8% by H2O2-oxidation, and 3.2% by direct photolysis). Radial distribution functions (RDF) provided information about the distribution of water around the CEF molecule. Aside from the RDF, investigation of intramolecular noncovalent interactions and calculations of bond dissociation energies for hydrogen abstraction enabled understanding of degradation mechanism of CEF. In order to investigate sensitivity of CEF towards the radical attacks, the concept of Fukui functions was used. The structures of intermediates and degradation pathways were suggested by UHPLC-LTQ OrbiTrap MS and density functional theory calculations. Toxicity assessments showed that intermediates formed during hydrolysis exerted only mild cell growth effects in selected cell lines.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Experimental and computational study of hydrolysis and photolysis of antibiotic ceftriaxone: Degradation kinetics, pathways, and toxicity
VL  - 768
DO  - 10.1016/j.scitotenv.2021.144991
SP  - 144991
ER  - 

@article{
author = "Abramović, Biljana F. and Uzelac, Maria M. and Armaković, Sanja J. and Gašić, Uroš and Četojević-Simin, Dragana D. and Armaković, Stevan",
year = "2021",
abstract = "In this work, we have experimentally and computationally investigated the process of hydrolysis and photolysis of cephalosporin antibiotics with ceftriaxone (CEF) as a model compound. The CEF hydrolysis was investigated in ultrapure and natural water, at 25 ± 1 °C and 4 ± 1 °C in the dark. It was found that CEF after 100 and 900 days at 25 ± 1°C and 4 ± 1 °C, respectively practically completely removed from ultrapure water. The CEF hydrolysis in natural water was five and three times slower at 25 ± 1 °C and 4 ± 1 °C, respectively than in ultrapure water. Further, the efficiency of direct photolysis (solar/UVA-B) and solar/H2O2 treatment of CEF was investigated. Under UVA-B radiation 95.6% of CEF was removed after 60 min, while for the same time of solar radiation degradation was practically not observed (only 3.2%). Also, the effects of different concentrations of H2O2 (0–150 mM) in the presence/absence of solar radiation were studied. The most efficient solar/H2O2 treatment was in the presence of 90 mM H2O2, whereby 66.8% of CEF was removed after 60 min (41.8% by indirect photolysis, 21.8% by H2O2-oxidation, and 3.2% by direct photolysis). Radial distribution functions (RDF) provided information about the distribution of water around the CEF molecule. Aside from the RDF, investigation of intramolecular noncovalent interactions and calculations of bond dissociation energies for hydrogen abstraction enabled understanding of degradation mechanism of CEF. In order to investigate sensitivity of CEF towards the radical attacks, the concept of Fukui functions was used. The structures of intermediates and degradation pathways were suggested by UHPLC-LTQ OrbiTrap MS and density functional theory calculations. Toxicity assessments showed that intermediates formed during hydrolysis exerted only mild cell growth effects in selected cell lines.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Experimental and computational study of hydrolysis and photolysis of antibiotic ceftriaxone: Degradation kinetics, pathways, and toxicity",
volume = "768",
doi = "10.1016/j.scitotenv.2021.144991",
pages = "144991"
}

Abramović, B. F., Uzelac, M. M., Armaković, S. J., Gašić, U., Četojević-Simin, D. D.,& Armaković, S.. (2021). Experimental and computational study of hydrolysis and photolysis of antibiotic ceftriaxone: Degradation kinetics, pathways, and toxicity. in Science of the Total Environment
Elsevier B.V.., 768, 144991.
https://doi.org/10.1016/j.scitotenv.2021.144991

Abramović BF, Uzelac MM, Armaković SJ, Gašić U, Četojević-Simin DD, Armaković S. Experimental and computational study of hydrolysis and photolysis of antibiotic ceftriaxone: Degradation kinetics, pathways, and toxicity. in Science of the Total Environment. 2021;768:144991.
doi:10.1016/j.scitotenv.2021.144991 .

Abramović, Biljana F., Uzelac, Maria M., Armaković, Sanja J., Gašić, Uroš, Četojević-Simin, Dragana D., Armaković, Stevan, "Experimental and computational study of hydrolysis and photolysis of antibiotic ceftriaxone: Degradation kinetics, pathways, and toxicity" in Science of the Total Environment, 768 (2021):144991,
https://doi.org/10.1016/j.scitotenv.2021.144991 . .

TY  - JOUR
AU  - Popović, Željko D.
AU  - Maier, Vítězslav
AU  - Avramov, Miloš
AU  - Uzelac, Iva
AU  - Gošić-Dondo, Snežana
AU  - Blagojević, Duško
AU  - Koštál, Vladimír
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.768593/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4689
AB  - The European corn borer Ostrinia nubilalis is a pest species, whose fifth instar larvae gradually develop cold hardiness during diapause. The physiological changes underlying diapause progression and cold hardiness development are still insufficiently understood in insects. Here, we follow a complex of changes related to energy metabolism during cold acclimation (5°C) of diapausing larvae and compare this to warm-acclimated (22°C) and non-diapause controls. Capillary electrophoresis of nucleotides and coenzymes has shown that in gradually cold-acclimated groups concentrations of ATP/ADP and, consequently, energy charge slowly decrease during diapause, while the concentration of AMP increases, especially in the first months of diapause. Also, the activity of cytochrome c oxidase (COX), as well as the concentrations of NAD + and GMP, decline in cold-acclimated groups, until the latter part of diapause, when they recover. Relative expression of NADH dehydrogenase ( nd1 ), coenzyme Q-cytochrome c reductase ( uqcr ), COX, ATP synthase ( atp ), ADP/ATP translocase ( ant ), and prohibitin 2 ( phb2 ) is supressed in cold-acclimated larvae during the first months of diapause and gradually increases toward the termination of diapause. Contrary to this, NADP + and UMP levels significantly increased in the first few months of diapause, after gradual cold acclimation, which is in connection with the biosynthesis of cryoprotective molecules, as well as regeneration of small antioxidants. Our findings evidence the existence of a cold-induced energy-saving program that facilitates long-term maintenance of larval diapause, as well as gradual development of cold hardiness. In contrast, warm acclimation induced faster depletion of ATP, ADP, UMP, NAD + , and NADP + , as well as higher activity of COX and generally higher expression of all energy-related genes in comparison to cold-acclimated larvae. Moreover, such unusually high metabolic activity, driven by high temperatures, lead to premature mortality in the warm-acclimated group after 2 months of diapause. Thus, our findings strongly support the importance of low temperature exposure in early diapause for gradual cold hardiness acquisition, successful maintenance of the resting state and return to active development. Moreover, they demonstrate potentially adverse effects of global climate changes and subsequent increase in winter temperatures on cold-adapted terrestrial organisms in temperate and subpolar regions.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Physiology
T1  - Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)
VL  - 12
DO  - 10.3389/fphys.2021.768593
SP  - 768593
ER  - 

@article{
author = "Popović, Željko D. and Maier, Vítězslav and Avramov, Miloš and Uzelac, Iva and Gošić-Dondo, Snežana and Blagojević, Duško and Koštál, Vladimír",
year = "2021",
abstract = "The European corn borer Ostrinia nubilalis is a pest species, whose fifth instar larvae gradually develop cold hardiness during diapause. The physiological changes underlying diapause progression and cold hardiness development are still insufficiently understood in insects. Here, we follow a complex of changes related to energy metabolism during cold acclimation (5°C) of diapausing larvae and compare this to warm-acclimated (22°C) and non-diapause controls. Capillary electrophoresis of nucleotides and coenzymes has shown that in gradually cold-acclimated groups concentrations of ATP/ADP and, consequently, energy charge slowly decrease during diapause, while the concentration of AMP increases, especially in the first months of diapause. Also, the activity of cytochrome c oxidase (COX), as well as the concentrations of NAD + and GMP, decline in cold-acclimated groups, until the latter part of diapause, when they recover. Relative expression of NADH dehydrogenase ( nd1 ), coenzyme Q-cytochrome c reductase ( uqcr ), COX, ATP synthase ( atp ), ADP/ATP translocase ( ant ), and prohibitin 2 ( phb2 ) is supressed in cold-acclimated larvae during the first months of diapause and gradually increases toward the termination of diapause. Contrary to this, NADP + and UMP levels significantly increased in the first few months of diapause, after gradual cold acclimation, which is in connection with the biosynthesis of cryoprotective molecules, as well as regeneration of small antioxidants. Our findings evidence the existence of a cold-induced energy-saving program that facilitates long-term maintenance of larval diapause, as well as gradual development of cold hardiness. In contrast, warm acclimation induced faster depletion of ATP, ADP, UMP, NAD + , and NADP + , as well as higher activity of COX and generally higher expression of all energy-related genes in comparison to cold-acclimated larvae. Moreover, such unusually high metabolic activity, driven by high temperatures, lead to premature mortality in the warm-acclimated group after 2 months of diapause. Thus, our findings strongly support the importance of low temperature exposure in early diapause for gradual cold hardiness acquisition, successful maintenance of the resting state and return to active development. Moreover, they demonstrate potentially adverse effects of global climate changes and subsequent increase in winter temperatures on cold-adapted terrestrial organisms in temperate and subpolar regions.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Physiology",
title = "Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)",
volume = "12",
doi = "10.3389/fphys.2021.768593",
pages = "768593"
}

Popović, Ž. D., Maier, V., Avramov, M., Uzelac, I., Gošić-Dondo, S., Blagojević, D.,& Koštál, V.. (2021). Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.). in Frontiers in Physiology
Lausanne: Frontiers Media S.A.., 12, 768593.
https://doi.org/10.3389/fphys.2021.768593

Popović ŽD, Maier V, Avramov M, Uzelac I, Gošić-Dondo S, Blagojević D, Koštál V. Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.). in Frontiers in Physiology. 2021;12:768593.
doi:10.3389/fphys.2021.768593 .

Popović, Željko D., Maier, Vítězslav, Avramov, Miloš, Uzelac, Iva, Gošić-Dondo, Snežana, Blagojević, Duško, Koštál, Vladimír, "Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.)" in Frontiers in Physiology, 12 (2021):768593,
https://doi.org/10.3389/fphys.2021.768593 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Čelić, Tatjana
AU  - Pihler, Ivan
AU  - Blagojević, Duško
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417&tlng=en
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4699
AB  - The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.
PB  - Parana, Brazil: Technology Institute of Paraná
T2  - Brazilian Archives of Biology and Technology
T1  - Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut
VL  - 64
DO  - 10.1590/1678-4324-2021200773
SP  - e21200773
ER  - 

@article{
author = "Vukašinović, Elvira and Purać, Jelena and Kojić, Danijela and Čelić, Tatjana and Pihler, Ivan and Blagojević, Duško",
year = "2021",
abstract = "The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.",
publisher = "Parana, Brazil: Technology Institute of Paraná",
journal = "Brazilian Archives of Biology and Technology",
title = "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut",
volume = "64",
doi = "10.1590/1678-4324-2021200773",
pages = "e21200773"
}

Vukašinović, E., Purać, J., Kojić, D., Čelić, T., Pihler, I.,& Blagojević, D.. (2021). Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology
Parana, Brazil: Technology Institute of Paraná., 64, e21200773.
https://doi.org/10.1590/1678-4324-2021200773

Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology. 2021;64:e21200773.
doi:10.1590/1678-4324-2021200773 .

Vukašinović, Elvira, Purać, Jelena, Kojić, Danijela, Čelić, Tatjana, Pihler, Ivan, Blagojević, Duško, "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut" in Brazilian Archives of Biology and Technology, 64 (2021):e21200773,
https://doi.org/10.1590/1678-4324-2021200773 . .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32777467
UR  - https://radar.ibiss.bg.ac.rs/123456789/3861
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
SP  - 108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852",
pages = "108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević D, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško, Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph." in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .