TY  - CONF
AU  - Škoro, Nikola
AU  - Jovanović, Olivera
AU  - Kumar, Amit
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Morina, Arian
AU  - Puač, Nevena
PY  - 2023
UR  - https://meetings.aps.org/Meeting/GEC23/Session/FF2.5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6427
AB  - Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.
PB  - American Physical Society
C3  - The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
T1  - Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6427
ER  - 

@conference{
author = "Škoro, Nikola and Jovanović, Olivera and Kumar, Amit and Živković, Suzana and Milutinović, Milica and Morina, Arian and Puač, Nevena",
year = "2023",
abstract = "Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.",
publisher = "American Physical Society",
journal = "The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA",
title = "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6427"
}

Škoro, N., Jovanović, O., Kumar, A., Živković, S., Milutinović, M., Morina, A.,& Puač, N.. (2023). Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
American Physical Society..
https://hdl.handle.net/21.15107/rcub_ibiss_6427

Škoro N, Jovanović O, Kumar A, Živković S, Milutinović M, Morina A, Puač N. Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

Škoro, Nikola, Jovanović, Olivera, Kumar, Amit, Živković, Suzana, Milutinović, Milica, Morina, Arian, Puač, Nevena, "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications" in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

TY  - JOUR
AU  - Kumar, Amit
AU  - Škoro, Nikola
AU  - Gernjak, Wolfgang
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Lumbaque, Elisabeth Cuervo
AU  - Farré, Maria José
AU  - Puač, Nevena
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0048969722082985
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5355
AB  - In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.
PB  - Elsevier B.V.
T2  - Science of The Total Environment
T1  - Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source
VL  - 864
DO  - 10.1016/j.scitotenv.2022.161194
SP  - 161194
ER  - 

@article{
author = "Kumar, Amit and Škoro, Nikola and Gernjak, Wolfgang and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Lumbaque, Elisabeth Cuervo and Farré, Maria José and Puač, Nevena",
year = "2023",
abstract = "In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.",
publisher = "Elsevier B.V.",
journal = "Science of The Total Environment",
title = "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source",
volume = "864",
doi = "10.1016/j.scitotenv.2022.161194",
pages = "161194"
}

Kumar, A., Škoro, N., Gernjak, W., Jovanović, O., Petrović, A., Živković, S., Lumbaque, E. C., Farré, M. J.,& Puač, N.. (2023). Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment
Elsevier B.V.., 864, 161194.
https://doi.org/10.1016/j.scitotenv.2022.161194

Kumar A, Škoro N, Gernjak W, Jovanović O, Petrović A, Živković S, Lumbaque EC, Farré MJ, Puač N. Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment. 2023;864:161194.
doi:10.1016/j.scitotenv.2022.161194 .

Kumar, Amit, Škoro, Nikola, Gernjak, Wolfgang, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Lumbaque, Elisabeth Cuervo, Farré, Maria José, Puač, Nevena, "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source" in Science of The Total Environment, 864 (2023):161194,
https://doi.org/10.1016/j.scitotenv.2022.161194 . .

TY  - CONF
AU  - Puač, Nevena
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - https://meetings.aps.org/Meeting/GEC22/Session/EF3.1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6323
AB  - The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.
PB  - American Physical Society
C3  - The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
T1  - Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells
SP  - EF3.00001
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6323
ER  - 

@conference{
author = "Puač, Nevena and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.",
publisher = "American Physical Society",
journal = "The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan",
title = "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells",
pages = "EF3.00001",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6323"
}

Puač, N., Jovanović, O., Petrović, A., Živković, S., Milutinović, M., Malović, G.,& Škoro, N.. (2022). Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
American Physical Society., EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323

Puač N, Jovanović O, Petrović A, Živković S, Milutinović M, Malović G, Škoro N. Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan. 2022;:EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

Puač, Nevena, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Škoro, Nikola, "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells" in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan (2022):EF3.00001,
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4938
AB  - Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.
PB  - Basel: MDPI
T2  - Plants
T1  - Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma
IS  - 7
VL  - 11
DO  - 10.3390/plants11070907
SP  - 907
ER  - 

@article{
author = "Škoro, Nikola and Živković, Suzana and Jevremović, Slađana and Puač, Nevena",
year = "2022",
abstract = "Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma",
number = "7",
volume = "11",
doi = "10.3390/plants11070907",
pages = "907"
}

Škoro, N., Živković, S., Jevremović, S.,& Puač, N.. (2022). Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants
Basel: MDPI., 11(7), 907.
https://doi.org/10.3390/plants11070907

Škoro N, Živković S, Jevremović S, Puač N. Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants. 2022;11(7):907.
doi:10.3390/plants11070907 .

Škoro, Nikola, Živković, Suzana, Jevremović, Slađana, Puač, Nevena, "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma" in Plants, 11, no. 7 (2022):907,
https://doi.org/10.3390/plants11070907 . .

TY  - CONF
AU  - Stevanović, Katarina
AU  - Pajić, Tanja
AU  - Živić, Miroslav
AU  - Krmpot, Aleksandar
AU  - Rabasović, Mihailo
AU  - Todorović, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5742
AB  - Prisustvo i aktivni karakter ćelijskog zida koji prekriva plazma membranu filamentoznih gljiva su do sad bili nesavladive prepreke da joj se pristupi elektrofiziološkim metodama na način koji bi omogućio kvalitetno registrovanje jonskih struja. Nedavno, mi smo razvili i optimizovali protokol koji omogućava kvalitetno snimanje struja, metodom nametnute voltaže na deliću membrane, sa plazma membrane protoplasta gljive oslobođenih od zida subćelijskom mikrohirurgijom korišćenjem femtosekundnog Ti:Sa lasera.1,2 Među registrovanim plazmamembranskim strujama protoplasta gljive dominantne su struje nošene različitim anjonskim vrstama. Na osnovu potencijala reverzije u asimetričnim jonskim uslovima identifikovani tipovi jonskih struja su, u najvećem broju slučajeva, bili slabo (42%) ili izrazito (35%) selektivni za hlor u prisustvu K, NO3, glutamatnog jona i visokih koncentracija Ca+2. Među nekoliko različitih struja slabije selektivnosti za hlor, daleko najzastupljenija je struja, konduktivnosti 21 ± 1 pS, prisutna u 42% svih kontakata registracije. Analiza aktivnosti pojedinačnih kanala ove, najzastupljenije, struje pokazuje da verovatnoća otvorenosti kanala slabo raste sa depolarizacijom membrane (Po(-80) = 0,3; Po(50) = 0,45) i opada na više depolarisanim vrednostima (Po(80) = 0,2). Učestalost paketića aktivnosti je najveća na hiperpolarisanim potencijalima (f(-80 mV) > 200 Hz) i dvostruko manja na depolarisanim (f(80 mV) < 100 Hz).
AB  - Присуство и активни карактер ћелијског зида који прекрива плазма мембрану
филаментозних гљива су до сад били несавладиве препреке да јој се приступи
електрофизиолошким методама на начин који би омогућио квалитетно
регистровање јонских струја. Недавно, ми смо развили и оптимизовали протокол
који омогућава квалитетно снимање струја, методом наметнуте волтаже на делићу
мембране, са плазма мембране протопласта гљиве ослобођених од зида
субћелијском микрохирургијом коришћењем фемтосекундног Ti:Sa ласера.1,2 Међу
регистрованим плазмамембранским струјама протопласта гљиве доминантне су
струје ношене различитим анјонским врстама. На основу потенцијала реверзије у
асиметричним јонским условима идентификовани типови јонских струја су, у
највећем броју случајева, били слабо (42%) или изразито (35%) селективни за хлор
у присуству K, NO3, глутаматног јона и високих концентрација Ca+2. Међу
неколико различитих струја слабије селективности за хлор, далеко најзаступљенија
је струја, кондуктивности 21 ± 1 pS, присутна у 42% свих контаката регистрације.
Анализа активности појединачних канала ове, најзаступљеније, струје показује да
вероватноћа отворености канала слабо расте са деполаризацијом мембране (Po(-80) =
0,3; Po(50) = 0,45) и опада на више деполарисаним вредностима (Po(80) = 0,2).
Учесталост пакетића активности је највећа на хиперполарисаним потенцијалима
(f(-80 mV) > 200 Hz) и двоструко мања на деполарисаним (f(80 mV) < 100 Hz).
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dominantne struje plazma membrane gljive Phycomyces blakesleeanus registrovane sa protoplasta dobijenih mikrohirurgijom ćelijskog zida femtosekundnim laserom
T1  - Доминантне струје плазма мембране гљиве Phycomyces blakesleeanus регистроване са протопласта добијених микрохирургијом ћелијског зида фемтосекундним ласером
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5742
ER  - 

@conference{
author = "Stevanović, Katarina and Pajić, Tanja and Živić, Miroslav and Krmpot, Aleksandar and Rabasović, Mihailo and Todorović, Nataša",
year = "2022",
abstract = "Prisustvo i aktivni karakter ćelijskog zida koji prekriva plazma membranu filamentoznih gljiva su do sad bili nesavladive prepreke da joj se pristupi elektrofiziološkim metodama na način koji bi omogućio kvalitetno registrovanje jonskih struja. Nedavno, mi smo razvili i optimizovali protokol koji omogućava kvalitetno snimanje struja, metodom nametnute voltaže na deliću membrane, sa plazma membrane protoplasta gljive oslobođenih od zida subćelijskom mikrohirurgijom korišćenjem femtosekundnog Ti:Sa lasera.1,2 Među registrovanim plazmamembranskim strujama protoplasta gljive dominantne su struje nošene različitim anjonskim vrstama. Na osnovu potencijala reverzije u asimetričnim jonskim uslovima identifikovani tipovi jonskih struja su, u najvećem broju slučajeva, bili slabo (42%) ili izrazito (35%) selektivni za hlor u prisustvu K, NO3, glutamatnog jona i visokih koncentracija Ca+2. Među nekoliko različitih struja slabije selektivnosti za hlor, daleko najzastupljenija je struja, konduktivnosti 21 ± 1 pS, prisutna u 42% svih kontakata registracije. Analiza aktivnosti pojedinačnih kanala ove, najzastupljenije, struje pokazuje da verovatnoća otvorenosti kanala slabo raste sa depolarizacijom membrane (Po(-80) = 0,3; Po(50) = 0,45) i opada na više depolarisanim vrednostima (Po(80) = 0,2). Učestalost paketića aktivnosti je najveća na hiperpolarisanim potencijalima (f(-80 mV) > 200 Hz) i dvostruko manja na depolarisanim (f(80 mV) < 100 Hz)., Присуство и активни карактер ћелијског зида који прекрива плазма мембрану
филаментозних гљива су до сад били несавладиве препреке да јој се приступи
електрофизиолошким методама на начин који би омогућио квалитетно
регистровање јонских струја. Недавно, ми смо развили и оптимизовали протокол
који омогућава квалитетно снимање струја, методом наметнуте волтаже на делићу
мембране, са плазма мембране протопласта гљиве ослобођених од зида
субћелијском микрохирургијом коришћењем фемтосекундног Ti:Sa ласера.1,2 Међу
регистрованим плазмамембранским струјама протопласта гљиве доминантне су
струје ношене различитим анјонским врстама. На основу потенцијала реверзије у
асиметричним јонским условима идентификовани типови јонских струја су, у
највећем броју случајева, били слабо (42%) или изразито (35%) селективни за хлор
у присуству K, NO3, глутаматног јона и високих концентрација Ca+2. Међу
неколико различитих струја слабије селективности за хлор, далеко најзаступљенија
је струја, кондуктивности 21 ± 1 pS, присутна у 42% свих контаката регистрације.
Анализа активности појединачних канала ове, најзаступљеније, струје показује да
вероватноћа отворености канала слабо расте са деполаризацијом мембране (Po(-80) =
0,3; Po(50) = 0,45) и опада на више деполарисаним вредностима (Po(80) = 0,2).
Учесталост пакетића активности је највећа на хиперполарисаним потенцијалима
(f(-80 mV) > 200 Hz) и двоструко мања на деполарисаним (f(80 mV) < 100 Hz).",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dominantne struje plazma membrane gljive Phycomyces blakesleeanus registrovane sa protoplasta dobijenih mikrohirurgijom ćelijskog zida femtosekundnim laserom, Доминантне струје плазма мембране гљиве Phycomyces blakesleeanus регистроване са протопласта добијених микрохирургијом ћелијског зида фемтосекундним ласером",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5742"
}

Stevanović, K., Pajić, T., Živić, M., Krmpot, A., Rabasović, M.,& Todorović, N.. (2022). Dominantne struje plazma membrane gljive Phycomyces blakesleeanus registrovane sa protoplasta dobijenih mikrohirurgijom ćelijskog zida femtosekundnim laserom. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5742

Stevanović K, Pajić T, Živić M, Krmpot A, Rabasović M, Todorović N. Dominantne struje plazma membrane gljive Phycomyces blakesleeanus registrovane sa protoplasta dobijenih mikrohirurgijom ćelijskog zida femtosekundnim laserom. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5742 .

Stevanović, Katarina, Pajić, Tanja, Živić, Miroslav, Krmpot, Aleksandar, Rabasović, Mihailo, Todorović, Nataša, "Dominantne struje plazma membrane gljive Phycomyces blakesleeanus registrovane sa protoplasta dobijenih mikrohirurgijom ćelijskog zida femtosekundnim laserom" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5742 .

TY  - CONF
AU  - Pajić, Tanja
AU  - Kozakijević, Suzana
AU  - Lukičić, Jovana
AU  - Živić, Miroslav
AU  - Krmpot, Aleksandar
AU  - Rabasović, Mihailo
AU  - Todorović, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5744
AB  - Biološka aktivnost selena zavisi od njegove doze i hemijskog oblika. U mikrotragovima povećava antioksidativni kapacitet, dok je u višim koncentracijama toksičan zbog stvaranja reaktivnih vrsta kiseonika i potencijalnog oštećenja mitohondrijalne membrane. Končaste gljive imaju sposobnost koncentrisanja selena u micelijumu i predstavljaju jedan od glavnih puteva za njegov ulazak u ekosisteme. U ovom radu je ispitivan uticaj hroničnog i akutnog dejstva natrijum selenita (uobičajen oblik selena u ishrani) na morfologiju i zastupljenost mitohondrija u živim ćelijama končaste gljive Phycomyces blakesleeanus tokom eksponencijalne faze rasta (EFR). Za in vivo oslikavanje morfologije mitohondrija i njihove dinamike u odgovoru na različite koncentracije selenita korišćena je multifotonska mikroskopija koja omogućava trodimenzionalno oslikavanje u visokoj rezoluciji i smanjeno fotooštećenje i fotoizbeljivanje uzorka zahvaljujući upotrebi infracrvenih ultrabrzih impulsnih lasera. Hronični tretmani selenitom su doveli do uočljivog trenda smanjenja zastupljenosti mitohondrija sa povećanjem koncentracije selenita. Izloženost hifa selenitu, tokom akutnih i hroničnih tretmana, rezultiralo je povećanjem zastupljenosti tubularne morfologije mitohondrija, koja je u akutnom tretmanu većim koncentracijama selenita (0,75 i 1 mM) bila povećana za 50% u odnosu na kontrolu. Kod mlađih hifa EFR izloženih hroničnom delovanju selenita (0,5 do 1 mM) tubularne mitohondrije su bile višestruko duže od onih u starijoj populaciji.
AB  - Биолошка активност селена зависи од његове дозе и хемијског облика. У
микротраговима повећава антиоксидативни капацитет, док је у вишим
концентрацијама токсичан због стварања реактивних врста кисеоника и
потенцијалног оштећења митохондријалне мембране. Кончасте гљиве имају
способност концентрисања селена у мицелијуму и представљају један од главних
путева за његов улазак у екосистеме. У овом раду је испитиван утицај хроничног и
акутног дејства натријум селенита (уобичајен облик селена у исхрани) на
морфологију и заступљеност митохондрија у живим ћелијама кончасте гљиве
Phycomyces blakesleeanus током експоненцијалне фазе раста (ЕФР). За in vivo
осликавање морфологије митохондрија и њихове динамике у одговору на
различите концентрације селенита коришћена је мултифотонска микроскопија која
омогућава тродимензионално осликавање у високој резолуцији и смањено
фотооштећење и фотоизбељивање узорка захваљујући употреби инфрацрвених
ултрабрзих импулсних ласера. Хронични третмани селенитом су довели до
уочљивог тренда смањења заступљености митохондрија са повећањем
концентрације селенита. Изложеност хифа селениту, током акутних и хроничних
третмана, резултирало је повећањем заступљености тубуларне морфологије
митохондрија, која је у акутном третману већим концентрацијама селенита (0,75 и
1 mМ) била повећана за 50% у односу на контролу. Код млађих хифа ЕФР
изложених хроничном деловању селенита (0,5 до 1 mМ) тубуларне митохондрије
су биле вишеструко дуже од оних у старијој популацији.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - In vivo oslikavanje pojedinačnih hifa Phycomyces blakesleeanus metodom multifotonske mikroskopije: uticaj selenita na morfologiju i funkciju mitohondrija
T1  - In vivo осликавање појединачних хифа Phycomyces blakesleeanus методом мултифотонске микроскопије: утицај селенита на морфологију и функцију митохондрија
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5744
ER  - 

@conference{
author = "Pajić, Tanja and Kozakijević, Suzana and Lukičić, Jovana and Živić, Miroslav and Krmpot, Aleksandar and Rabasović, Mihailo and Todorović, Nataša",
year = "2022",
abstract = "Biološka aktivnost selena zavisi od njegove doze i hemijskog oblika. U mikrotragovima povećava antioksidativni kapacitet, dok je u višim koncentracijama toksičan zbog stvaranja reaktivnih vrsta kiseonika i potencijalnog oštećenja mitohondrijalne membrane. Končaste gljive imaju sposobnost koncentrisanja selena u micelijumu i predstavljaju jedan od glavnih puteva za njegov ulazak u ekosisteme. U ovom radu je ispitivan uticaj hroničnog i akutnog dejstva natrijum selenita (uobičajen oblik selena u ishrani) na morfologiju i zastupljenost mitohondrija u živim ćelijama končaste gljive Phycomyces blakesleeanus tokom eksponencijalne faze rasta (EFR). Za in vivo oslikavanje morfologije mitohondrija i njihove dinamike u odgovoru na različite koncentracije selenita korišćena je multifotonska mikroskopija koja omogućava trodimenzionalno oslikavanje u visokoj rezoluciji i smanjeno fotooštećenje i fotoizbeljivanje uzorka zahvaljujući upotrebi infracrvenih ultrabrzih impulsnih lasera. Hronični tretmani selenitom su doveli do uočljivog trenda smanjenja zastupljenosti mitohondrija sa povećanjem koncentracije selenita. Izloženost hifa selenitu, tokom akutnih i hroničnih tretmana, rezultiralo je povećanjem zastupljenosti tubularne morfologije mitohondrija, koja je u akutnom tretmanu većim koncentracijama selenita (0,75 i 1 mM) bila povećana za 50% u odnosu na kontrolu. Kod mlađih hifa EFR izloženih hroničnom delovanju selenita (0,5 do 1 mM) tubularne mitohondrije su bile višestruko duže od onih u starijoj populaciji., Биолошка активност селена зависи од његове дозе и хемијског облика. У
микротраговима повећава антиоксидативни капацитет, док је у вишим
концентрацијама токсичан због стварања реактивних врста кисеоника и
потенцијалног оштећења митохондријалне мембране. Кончасте гљиве имају
способност концентрисања селена у мицелијуму и представљају један од главних
путева за његов улазак у екосистеме. У овом раду је испитиван утицај хроничног и
акутног дејства натријум селенита (уобичајен облик селена у исхрани) на
морфологију и заступљеност митохондрија у живим ћелијама кончасте гљиве
Phycomyces blakesleeanus током експоненцијалне фазе раста (ЕФР). За in vivo
осликавање морфологије митохондрија и њихове динамике у одговору на
различите концентрације селенита коришћена је мултифотонска микроскопија која
омогућава тродимензионално осликавање у високој резолуцији и смањено
фотооштећење и фотоизбељивање узорка захваљујући употреби инфрацрвених
ултрабрзих импулсних ласера. Хронични третмани селенитом су довели до
уочљивог тренда смањења заступљености митохондрија са повећањем
концентрације селенита. Изложеност хифа селениту, током акутних и хроничних
третмана, резултирало је повећањем заступљености тубуларне морфологије
митохондрија, која је у акутном третману већим концентрацијама селенита (0,75 и
1 mМ) била повећана за 50% у односу на контролу. Код млађих хифа ЕФР
изложених хроничном деловању селенита (0,5 до 1 mМ) тубуларне митохондрије
су биле вишеструко дуже од оних у старијој популацији.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "In vivo oslikavanje pojedinačnih hifa Phycomyces blakesleeanus metodom multifotonske mikroskopije: uticaj selenita na morfologiju i funkciju mitohondrija, In vivo осликавање појединачних хифа Phycomyces blakesleeanus методом мултифотонске микроскопије: утицај селенита на морфологију и функцију митохондрија",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5744"
}

Pajić, T., Kozakijević, S., Lukičić, J., Živić, M., Krmpot, A., Rabasović, M.,& Todorović, N.. (2022). In vivo oslikavanje pojedinačnih hifa Phycomyces blakesleeanus metodom multifotonske mikroskopije: uticaj selenita na morfologiju i funkciju mitohondrija. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_5744

Pajić T, Kozakijević S, Lukičić J, Živić M, Krmpot A, Rabasović M, Todorović N. In vivo oslikavanje pojedinačnih hifa Phycomyces blakesleeanus metodom multifotonske mikroskopije: uticaj selenita na morfologiju i funkciju mitohondrija. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_5744 .

Pajić, Tanja, Kozakijević, Suzana, Lukičić, Jovana, Živić, Miroslav, Krmpot, Aleksandar, Rabasović, Mihailo, Todorović, Nataša, "In vivo oslikavanje pojedinačnih hifa Phycomyces blakesleeanus metodom multifotonske mikroskopije: uticaj selenita na morfologiju i funkciju mitohondrija" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):28,
https://hdl.handle.net/21.15107/rcub_ibiss_5744 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Zoran Lj.
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5268
AB  - The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5268
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Petrović, Zoran Lj. and Škoro, Nikola and Puač, Nevena",
year = "2022",
abstract = "The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5268"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Petrović, Z. Lj., Škoro, N.,& Puač, N.. (2022). Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268

Živković S, Jevremović S, Gašić U, Milutinović M, Petrović ZL, Škoro N, Puač N. Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Petrović, Zoran Lj., Škoro, Nikola, Puač, Nevena, "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):137,
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Devrnja, Nina
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Puač, Nevena
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5269
AB  - Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Molecular response to PAW in model plant species
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5269
ER  - 

@conference{
author = "Milutinović, Milica and Jovanović, Olivera and Devrnja, Nina and Todorović, Miloš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Puač, Nevena and Škoro, Nikola",
year = "2022",
abstract = "Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Molecular response to PAW in model plant species",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5269"
}

Milutinović, M., Jovanović, O., Devrnja, N., Todorović, M., Živković, S., Savić, J., Skorić, M., Puač, N.,& Škoro, N.. (2022). Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269

Milutinović M, Jovanović O, Devrnja N, Todorović M, Živković S, Savić J, Skorić M, Puač N, Škoro N. Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

Milutinović, Milica, Jovanović, Olivera, Devrnja, Nina, Todorović, Miloš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Puač, Nevena, Škoro, Nikola, "Molecular response to PAW in model plant species" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

TY  - CONF
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5271
AB  - The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.
C3  - Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands
T1  - Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5271
ER  - 

@conference{
author = "Puač, Nevena and Živković, Suzana and Milutinović, Milica and Jovanović, Olivera and Petrović, Anđelija and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.",
journal = "Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands",
title = "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions",
pages = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5271"
}

Puač, N., Živković, S., Milutinović, M., Jovanović, O., Petrović, A., Malović, G.,& Škoro, N.. (2022). Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands, 16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271

Puač N, Živković S, Milutinović M, Jovanović O, Petrović A, Malović G, Škoro N. Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands. 2022;:16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

Puač, Nevena, Živković, Suzana, Milutinović, Milica, Jovanović, Olivera, Petrović, Anđelija, Malović, Gordana, Škoro, Nikola, "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions" in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands (2022):16,
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4414
AB  - Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.
PB  - National Institute for Lasers, Plasma and Radiation Physics
PB  - Alexandru Ioan Cuza University
PB  - Romanian Physical Society
C3  - 19th International conference on plasma physics and applications, CPPA 2021
T1  - Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4414
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2021",
abstract = "Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.",
publisher = "National Institute for Lasers, Plasma and Radiation Physics, Alexandru Ioan Cuza University, Romanian Physical Society",
journal = "19th International conference on plasma physics and applications, CPPA 2021",
title = "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4414"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Puač, N., Škoro, N.,& Petrović, Z. L.. (2021). Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021
National Institute for Lasers, Plasma and Radiation Physics., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414

Živković S, Jevremović S, Gašić U, Milutinović M, Puač N, Škoro N, Petrović ZL. Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli" in 19th International conference on plasma physics and applications, CPPA 2021 (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

TY  - CONF
AU  - Živković, Suzana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4396
AB  - Recent scientific, technological and methodological advances in the fields of plasma physics and plant
physiology offer a wide range of possibilities for employment of plasma technology in treatment of
plants. Application of PAW or direct plasma treatment represent an alternative and valuable approach
to plant biotechnology being chemically-free, energy efficient, and environmentally friendly. However,
the distinct mechanisms responsible for the positive effects of plasma treatment on plant cells are
still vague. Plant calli cultures (calli grown aseptically on semisolid agar medium) and calli suspension
cultures (calli grown aseptically in liquid medium in test tubes, shake flasks, or bioreactors) with or
without addition of plant growth regulators are used to illustrate changes in physiological response of
plant cells/tissues induced by plasma treatment. Key challenges and obstacles to achieve precise and
localized in vivo treatments of living cells and tissues will be discussed in light of current knowledge in
the field.
PB  - Sarajevo: University of East Sarajevo
PB  - Zvornik: Faculty of Technology
C3  - I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina
T1  - Plasma treatment of plants: puzzle games for biologists and physicists
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4396
ER  - 

@conference{
author = "Živković, Suzana",
year = "2021",
abstract = "Recent scientific, technological and methodological advances in the fields of plasma physics and plant
physiology offer a wide range of possibilities for employment of plasma technology in treatment of
plants. Application of PAW or direct plasma treatment represent an alternative and valuable approach
to plant biotechnology being chemically-free, energy efficient, and environmentally friendly. However,
the distinct mechanisms responsible for the positive effects of plasma treatment on plant cells are
still vague. Plant calli cultures (calli grown aseptically on semisolid agar medium) and calli suspension
cultures (calli grown aseptically in liquid medium in test tubes, shake flasks, or bioreactors) with or
without addition of plant growth regulators are used to illustrate changes in physiological response of
plant cells/tissues induced by plasma treatment. Key challenges and obstacles to achieve precise and
localized in vivo treatments of living cells and tissues will be discussed in light of current knowledge in
the field.",
publisher = "Sarajevo: University of East Sarajevo, Zvornik: Faculty of Technology",
journal = "I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina",
title = "Plasma treatment of plants: puzzle games for biologists and physicists",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4396"
}

Živković, S.. (2021). Plasma treatment of plants: puzzle games for biologists and physicists. in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina
Sarajevo: University of East Sarajevo., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_4396

Živković S. Plasma treatment of plants: puzzle games for biologists and physicists. in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina. 2021;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_4396 .

Živković, Suzana, "Plasma treatment of plants: puzzle games for biologists and physicists" in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina (2021):8,
https://hdl.handle.net/21.15107/rcub_ibiss_4396 .