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Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures
dc.creator | Otašević, Vesna | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Korac, Aleksandra B | |
dc.creator | Stančić, Ana | |
dc.creator | Janković, Aleksandra | |
dc.creator | Vučetić, Milica | |
dc.creator | Korać, Bato | |
dc.date.accessioned | 2017-11-23T11:08:36Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2011 | sr |
dc.identifier.issn | 0306-4565 | sr |
dc.identifier.other | Rad_konverzija_3276 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1281 | |
dc.description.abstract | Study elucidates molecular mechanisms underlying activation of catalase and glutathione peroxidase (GSH-Px) during cold-exposure by examining time-dependent changes in their mRNA, protein expression and activity, in interscapular brown adipose tissue (IBAT) of rats kept at room temperature (22 +/- 1 degrees C), or subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days. To examine involvement of nitric oxide (*NO) in catalase and GSH-Px regulation, rats were treated with L-arginine or N((1))-nitro-L-arginine-methyl ester (L-NAME). In all groups, cold increased catalase mRNA, protein expression and activity from day 3 to day 45 of acclimation. However, cold-acclimation increased GSH-Px mRNA content on day 3, protein content from day 1 to day 45 and activity no sooner than on day 45. At room temperature, 21- and 45-day L-arginine and L-NAME supplementation increased catalase and GSH-Px mRNA expression, but decreased their activity. Concomitantly, catalase protein content decreased, while GSH-Px protein expression increased compared to control. Data show that cold is dominant stimulus that determines IBAT catalase and GSH-Px responses, by setting tissue metabolic state. In thermogenic-active tissue, catalase is regulated on transcriptional level, while GSH-Px is regulated post-translationally. On the other side, it was shown that *NO affects catalase and GSH-Px only in thermogenic-inactive tissue. (C) 2011 Elsevier Ltd. All rights reserved. | en |
dc.description.sponsorship | Ministry of Science and Technological Development of the Republic of Serbia [173055]; COST, Action [FA0602] | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Journal of Thermal Biology | sr |
dc.title | Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Бузаджић, Биљана Ј.; Оташевић, Весна; Кораћ, Бато М.; Вучетић, Милица; Јанковић, Aлександра; Станчић, Aна; Корац, Aлександра Б; | |
dc.citation.issue | 5 | sr |
dc.citation.volume | 36 | sr |
dc.citation.epage | 276 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1281 |
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