dc.creator | Ninković, Slavica | |
dc.creator | Ćosić, Tatjana | |
dc.creator | Vinterhalter, Branka | |
dc.creator | Uzelac, Branka | |
dc.creator | Cingel, Aleksandar | |
dc.creator | Savić, Jelena M. | |
dc.creator | Radović, Svetlana R | |
dc.date.accessioned | 2017-11-23T11:10:35Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2010 | sr |
dc.identifier.issn | 0167-6857 | sr |
dc.identifier.other | Rad_konverzija_3335 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1340 | |
dc.description.abstract | Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet. | en |
dc.description.sponsorship | Ministry of Science and Technology of Serbia [143026 B] | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Plant Cell Tissue and Organ Culture | sr |
dc.title | Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Узелац, Бранка Б; Нинковић, Славица Б.; Ђорђевић, Татјана; Винтерхалтер, Бранка С.; Цингел, Aлександар Д.; Савић, Јелена М.; Радовић, Светлана Р; | |
dc.citation.issue | 1 | sr |
dc.citation.volume | 103 | sr |
dc.citation.epage | 91 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1340 | |