dc.creator | Janković, Aleksandra | |
dc.creator | Golic, Igor | |
dc.creator | Markelic, Milica | |
dc.creator | Stančić, Ana | |
dc.creator | Otašević, Vesna | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Korac, Aleksandra | |
dc.creator | Korać, Bato | |
dc.date.accessioned | 2016-05-23T10:59:41Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1469-7793 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1921 | |
dc.description.abstract | Conversion of white into brown adipose tissue may have important
implications in obesity resistance and treatment. Several browning
agents or conditions ignite thermogenesis in white adipose tissue (WAT).
To reveal the capacity of WAT to function in a brownish/burning mode
over the long term, we investigated the progression of the rat
retroperitoneal WAT (rpWAT) browning during 45days of cold acclimation.
During the early stages of cold acclimation, the majority of rpWAT
adipocytes underwent multilocularization and thermogenic-profile
induction, as demonstrated by the presence of a multitude of uncoupling
protein 1 (UCP1)-immunopositive paucilocular adipocytes containing
peroxisome proliferator-activated receptor (PPAR) coactivator-1 (PGC-1)
and PR domain-containing 16 (PRDM16) in their nuclei. After 45days, all
adipocytes remained PRDM16 immunopositive, but only a few multilocular
adipocytes rich in mitochondria remained UCP1/PGC-1 immunopositive.
Molecular evidence showed that thermogenic recruitment of rpWAT occurred
following cold exposure, but returned to starting levels after cold
acclimation. Compared with controls (22 +/- 1 degrees C), levels of UCP1
mRNA increased in parallel with PPAR (PPAR from days 1 to 7 and PGC-1 on
day 1). Transcriptional recruitment of rpWAT was followed by an increase
in UCP1 protein content (from days 1 to 21). Results clearly showed that
most of the adipocytes within rpWAT underwent transient brown-fat-like
thermogenic recruitment upon stimulation, but only a minority of cells
retained a brown adipose tissue-like phenotype after the attainment of
cold acclimation. Therefore, browning of WAT is dependent on both
maintaining the thermogenic response and retaining enough brown-like
thermogenically competent adipocytes in the long-term. Both aspects of
browning could be important for long-term energy homeostasis and
body-weight regulation. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}173054, 173055] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Journal of Physiology-London | |
dc.title | Two key temporally distinguishable molecular and cellular components of
white adipose tissue browning during cold acclimation | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Станциц, Aна; Корац, Aлександра; Бузаджић, Биљана Ј.; Оташевић, Весна; Голиц, Игор; Маркелиц, Милица; Кораћ, Бато М.; Јанковић, Aлександра; | |
dc.citation.issue | 15 | |
dc.citation.volume | 593 | |
dc.identifier.doi | 10.1113/JP270805 | |
dc.identifier.scopus | 2-s2.0-84936877196 | |
dc.identifier.wos | 000358789800010 | |
dc.citation.spage | 3267 | |
dc.citation.epage | 3280 | |
dc.type.version | publishedVersion | en |