dc.creator | Rajičić, Marija | |
dc.creator | Adnađević, Tanja | |
dc.creator | Stamenković, Gorana | |
dc.creator | Blagojević, Jelena | |
dc.creator | Vujošević, Mladen | |
dc.date.accessioned | 2016-05-23T11:00:05Z | |
dc.date.available | 2016-05-23T11:00:05Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1820-6069 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2065 | |
dc.description.abstract | B chromosomes (Bs) are a very heterogeneous group of extra chromosomes.
In various species Bs occur with different nucleotide sequences ranging
from repetitive to protein coding. In yellow-necked field mice, Apodemus
flavicollis Bs are small euchromatic chromosomes and untill now, only
few molecular analyses have been conducted. In this study we examined A.
flavicollis individuals with different number of Bs for presence of two
genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in
three Canidae species and one Cervidae species. This gene is a coding
receptor critical for proliferation and cell differentiation of
hematopoietic, melanoblast and primordial germ cells, and is highly
conserved within mammals. While using semiquantitative PCR, we did not
notice any difference in the C-KIT band intensity among animals with
different number of Bs (0-3). The presence of only one copy of C-KIT
gene was confirmed using real time-PCR on genomic DNA of A. flavicollis
specimens with different number of Bs. rRNA genes in eukaryotes' genome
are organized like units of tandem repeated sequences. The units form
distinct clusters on one to several chromosome pairs. rRNA genes were
found on Bs in different species including two species of genus
Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA
gene about three times that of the calibrator 0 B sample. This result
can indicate the presence of 18S rRNA gene on Bs, but its confirmation
requires the implementation of other methods. Still, we can neither
confirm nor deny the existence of pseudogen of tested target genes, or
lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our
findings are further discussed. | en |
dc.description.sponsorship | Ministry of Science and Technological Development of the Republic of
Serbia {[}173003] | |
dc.language | English | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173003/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Genetika-Belgrade | |
dc.subject | Apodemus flavicollis | |
dc.subject | B chromosomes | |
dc.subject | semiquantitative
PCR | |
dc.title | Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Стаменковић, Горана; Вујосевиц, Младен; Благојевић, Јелена; Рајициц, Марија; Aднађевић, Тања Б.; | |
dc.rights.holder | © Serbian Genetics Society | |
dc.citation.issue | 1 | |
dc.citation.volume | 47 | |
dc.identifier.doi | 10.2298/GENSR1501311R | |
dc.identifier.scopus | 2-s2.0-84929303311 | |
dc.identifier.wos | 000355248900028 | |
dc.citation.spage | 311 | |
dc.citation.epage | 321 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/5605/0534-00121501311R.pdf | |