dc.creator | Misic, Danijela | |
dc.creator | Šiler, Branislav | |
dc.creator | Gasic, Uros | |
dc.creator | Avramov, Stevan | |
dc.creator | Živković, Suzana | |
dc.creator | Nestorović Živković, Jasmina | |
dc.creator | Milutinović, Milica | |
dc.creator | Tesic, Zivoslav | |
dc.date.accessioned | 2016-05-23T11:00:10Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0958-0344 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2087 | |
dc.description.abstract | Introduction - Nepeta species contain a variety of secondary
metabolites, including iridoid monoterpenes - nepetalactones and
phenolic acids - that are considered the main bioactive constituents.
This work represents the first attempt to comparatively explore
variations in these two major groups of secondary metabolites within the
genus.
Objective - To develop an efficient analytical methodology for
simultaneous analysis of nepetalactones and phenolic acids in methanol
extracts of selected Nepeta species, and to evaluate its potential
application in chemotaxonomic studies.
Material and methods - A UHPLC combined with linear-trap quadrupole
(LTQ) orbitrap MS method was used to characterise chemical diversity and
complexity of phenolics among 12 selected Nepeta species. A targeted
metabolomic approach using UHPLC coupled to a diode array detector (DAD)
and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was
developed and validated for quantitative analysis of six hydroxycinnamic
acid derivatives and four nepetalactones.
Results - Phenolic profiling provided a valuable database of bioactive
compounds in the plant group studied, including phenolic acids
(hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones,
flavonols and flavanones). Principal component analysis and cluster
analysis suggested the applicability of 10 targeted compounds as
chemomarkers for chemotaxonomic studies. Pearson's correlation analysis
revealed significant positive correlations between metabolites involved
in different biosynthetic pathways (phenylpropanoid or monoterpenoid).
Conclusion - The described targeted metabolomic approach proved to be
highly beneficial in designing a phytochemical overview of the genus
Nepeta, and might have applications in further clarification of
phylogenetic relations. Furthermore, it has the potential to be
implemented in a routine quality control of plant material and herbal
preparations. Copyright (C) 2014 John Wiley \& Sons, Ltd. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}173024, 172017]; FP7 RegPot project FCUB ERA GA
{[}256716] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Phytochemical Analysis | |
dc.subject | Chemotaxonomy | |
dc.subject | targeted metabolomic analysis | |
dc.subject | nepetalactone | |
dc.subject | phenolic
acids | |
dc.subject | catmint | |
dc.subject | catnip | |
dc.subject | Lamiaceae | |
dc.subject | Nepeta | |
dc.title | Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids
and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible
Application in Chemotaxonomic Studies | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aврамов, Стеван Н.; Гасиц, Урос; Тесиц, Зивослав; Мисиц, Данијела; Милутиновиц, Милица; Зивковиц, Јасмина Несторовиц; Шилер, Бранислав; Зивковиц, Сузана; | |
dc.citation.issue | 1 | |
dc.citation.volume | 26 | |
dc.identifier.doi | 10.1002/pca.2538 | |
dc.identifier.scopus | 2-s2.0-84917691460 | |
dc.identifier.wos | 000349963000010 | |
dc.citation.spage | 72 | |
dc.citation.epage | 85 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 | |