dc.creator | Sarac, Zorica | |
dc.creator | Matejic, Jelena S. | |
dc.creator | Stojanovic-Radic, Zorica Z. | |
dc.creator | Veselinovic, Jovana B. | |
dc.creator | Džamić, Ana M. | |
dc.creator | Bojović, Srđan | |
dc.creator | Marin, Petar D. | |
dc.date.accessioned | 2016-05-23T11:00:17Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1879-0534 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2127 | |
dc.description.abstract | In the current work, in vitro antioxidant, antibacterial, and antifungal
activites of the needle terpenes of three taxa of Pinus nigra from
Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
The black pine essential oils showed generally weak antioxidative
properties tested by two methods (DPPH and ABTS scavenging assays),
where the highest activity was identified in P. nigra var. banatica
(IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
aureus and Bacillus cereus) showed sensitivity against essential oils of
all three P. nigra taxa. The tested oils have been shown to possess
inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
banatica exhibited the highest and ssp. nigra the lowest antimicrobial
action. In order to determine potential compounds that are responsible
for alternative mode of action, molecular docking simulations inside
FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
were the most abundant terpenoid (germacrene D-4-ol) and its
structurally similar terpene (germacrene D), both present in all three
essential oils. It was determined that the oxygenated form of the
molecule creates stable bonds with investigated enzyme FtsZ, and that
this compound, through this mechanism of action participates in the
antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education and Science of the Republic of Serbia {[}173029] | en |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Computers in Biology and Medicine | |
dc.subject | Pinus nigra | |
dc.subject | Needle terpenes | |
dc.subject | Biological activity | |
dc.subject | Bacillus cereus | |
dc.subject | Molecular docking | |
dc.title | Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
inhibition by selected compounds as contribution to their antimicrobial
activity | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Стојановиц-Радиц, Зорица З.; Бојовић, Срђан; Веселиновиц, Јована Б.; Сарац, Зорица; Матејиц, Јелена С.; Марин, Петар Д.; Джамић, Aна М.; | |
dc.citation.volume | 54 | |
dc.identifier.doi | 10.1016/j.compbiomed.2014.08.022 | |
dc.identifier.scopus | 2-s2.0-84937107510 | |
dc.identifier.wos | 000345189800009 | |
dc.citation.spage | 72 | |
dc.citation.epage | 78 | |
dc.type.version | publishedVersion | en |