dc.creator | Dragicevic, Milan | |
dc.creator | Todorović, Slađana | |
dc.creator | Bogdanović, Milica | |
dc.creator | Filipović, Biljana | |
dc.creator | Music, Danijela | |
dc.creator | Simonović, Ana | |
dc.date.accessioned | 2016-05-23T11:00:33Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0981-9428 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2209 | |
dc.description.abstract | Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation,
which catalyzes the formation of glutamine from ammonia and glutamate.
Plant GS isoforms are multimeric enzymes, recently shown to be decamers.
The Arabidopsis genome encodes five cytosolic (GS1) proteins labeled as
GLN1;1 through GLN1; 5 and one chloroplastic (GS2) isoform, GLN2;0.
However, as many as 11 GS activity bands were resolved from different
Arabidopsis tissues by Native PAGE and activity staining. Western
analysis showed that all 11 isoforms are composed exclusively of 40 kDa
GS1 subunits. Of five GS1 genes, only GLN1;1, GLN1;2 and GLN1;3
transcripts accumulated to significant levels in vegetative tissues,
indicating that only subunits encoded by these three genes produce the
11-band zymogram. Even though the GS2 gene also had significant
expression, the corresponding activity was not detected, probably due to
inactivation. To resolve the subunit composition of 11 active GS1
isoforms, homozygous knockout mutants deficient in the expression of
different GS1 genes were selected from the progeny of T-DNA insertional
SALK and SAIL lines. Comparison of GS isoenzyme patterns of the selected
GS1 knockout mutants indicated that all of the detected isoforms consist
of varying proportions of GLN1;1, GLN1;2 and GLN1;3 subunits, and that
GLN1 :1 and GLN1;3, as well as GLN1;2 and GLN1;3 and possibly GLN1;1 and
GLN1;2 proteins combine in all proportions to form active homo- and
heterodecamers. (C) 2014 Elsevier Masson SAS. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}ON173024] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Plant Physiology and Biochemistry | |
dc.subject | Glutamine synthetase | |
dc.subject | Knockout mutants | |
dc.subject | T-DNA insertion | |
dc.subject | Subunit
composition | |
dc.subject | Arabidopsis thaliana | |
dc.subject | Isoforms | |
dc.title | Knockout mutants as a tool to identify the subunit composition of
Arabidopsis glutamine synthetase isoforms | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Симоновиц, Aна; Богдановиц, Милица; Филиповиц, Биљана; Мусиц, Данијела; Драгицевиц, Милан; Тодоровиц, Сладана; | |
dc.citation.volume | 79 | |
dc.identifier.doi | 10.1016/j.plaphy.2014.02.023 | |
dc.identifier.scopus | 2-s2.0-84896481926 | |
dc.identifier.wos | 000335618500001 | |
dc.citation.spage | 1 | |
dc.citation.epage | 9 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 | |