dc.creator | Velickovic, Ksenija | |
dc.creator | Markelic, Milica | |
dc.creator | Golic, Igor | |
dc.creator | Otašević, Vesna | |
dc.creator | Stančić, Ana | |
dc.creator | Janković, Aleksandra | |
dc.creator | Vucetic, Milica | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Korać, Bato | |
dc.creator | Korac, Aleksandra | |
dc.date.accessioned | 2016-05-23T11:00:36Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1436-6215 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2227 | |
dc.description.abstract | Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are
important intestinal neurotransmitters that coexist in the gut enteric
nervous system and play an important role in intestinal physiology
(e.g., absorption, motility, fluid secretion and smooth muscle
relaxation). It is also known that cold exposure alters several aspects
of gastrointestinal physiology and induces hyperphagia to meet increased
metabolic demands, but there are no data regarding NO and VIP
involvement in intestinal response during acclimation to cold. The
objective of this study was to determine the influence of long-term
l-arginine supplementation on the expression of the three isoforms of
nitric oxide synthase (NOS) and VIP in small intestine of rats
acclimated to room temperature or cold.
Animals (six per group) acclimated to room temperature (22 +/- A 1 A
degrees C) and cold (4 +/- A 1 A degrees C), respectively, were treated
with 2.25 \% l-arginine, a substrate for NOSs, or with 0.01 \% N
(omega)-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45
days. The topographical distribution of VIP and NOSs expression in small
intestine was studied by immunohistochemistry, and ImageJ software was
used for semiquantitative densitometric analysis of their
immunoexpression.
Long-term dietary l-arginine supplementation increases VIP and NOSs
immunoexpression at room temperature while at cold increases the
endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat
small intestine.
Our results demonstrate that long-term dietary l-arginine
supplementation modulates NOSs and VIP immunoexpression in rat small
intestine with respect to ambient temperature, pointing out the eNOS as
a predominant NOS isoform with an immunoexpression pattern similar to
VIP. | en |
dc.description.sponsorship | Serbian Ministry of Education, Science and Technological Development
{[}173055] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | European Journal of Nutrition | |
dc.subject | Arginine | |
dc.subject | Small intestine | |
dc.subject | Nitric oxide synthase | |
dc.subject | Vasoactive intestinal
peptide | |
dc.title | Long-term dietary L-arginine supplementation increases endothelial
nitric oxide synthase and vasoactive intestinal peptide immunoexpression
in rat small intestine | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Корац, Aлександра; Маркелиц, Милица; Голиц, Игор; Оташевић, Весна; Станциц, Aна; Јанковић, Aлександра; Вуцетиц, Милица; Бузаджић, Биљана Ј.; Кораћ, Бато М.; Велицковиц, Ксенија; | |
dc.citation.issue | 3 | |
dc.citation.volume | 53 | |
dc.identifier.doi | 10.1007/s00394-013-0585-8 | |
dc.identifier.scopus | 2-s2.0-84928706558 | |
dc.identifier.wos | 000334054400012 | |
dc.citation.spage | 813 | |
dc.citation.epage | 821 | |
dc.type.version | publishedVersion | en |