dc.creator | Trikka, Fotini A. | |
dc.creator | Nikolaidis, Alexandros | |
dc.creator | Ignea, Codruta | |
dc.creator | Tsaballa, Aphrodite | |
dc.creator | Tziveleka, Leto-Aikaterini | |
dc.creator | Ioannou, Efstathia | |
dc.creator | Roussis, Vassilios | |
dc.creator | Stea, Eleni A. | |
dc.creator | Božić, Dragana | |
dc.creator | Argiriou, Anagnostis | |
dc.creator | Kanellis, Angelos K. | |
dc.creator | Kampranis, Sotirios C. | |
dc.creator | Makris, Antonios M. | |
dc.date.accessioned | 2016-05-23T11:00:53Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2335 | |
dc.description.abstract | Background: Salvia diterpenes have been found to have health promoting
properties. Among them, carnosic acid and carnosol, tanshinones and
sclareol are well known for their cardiovascular, antitumor,
antiinflammatory and antioxidant activities. However, many of these
compounds are not available at a constant supply and developing
biotechnological methods for their production could provide a
sustainable alternative. The transcriptome of S. pomifera glandular
trichomes was analysed aiming to identify genes that could be used in
the engineering of synthetic microbial systems.
Results: In the present study, a thorough metabolite analysis of S.
pomifera leaves led to the isolation and structure elucidation of
carnosic acid-family metabolites including one new natural product.
These labdane diterpenes seem to be synthesized through miltiradiene and
ferruginol. Transcriptomic analysis of the glandular trichomes from the
S. pomifera leaves revealed two genes likely involved in miltiradiene
synthesis. Their products were identified and the corresponding enzymes
were characterized as copalyl diphosphate synthase (SpCDS) and
miltiradiene synthase (SpMilS). In addition, several CYP-encoding
transcripts were identified providing a valuable resource for the
identification of the biosynthetic mechanism responsible for the
production of carnosic acid-family metabolites in S. pomifera.
Conclusions: Our work has uncovered the key enzymes involved in
miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The
transcriptomic dataset obtained provides a valuable tool for the
identification of the CYPs involved in the synthesis of carnosic
acid-family metabolites. | en |
dc.description.sponsorship | General Secretariat of Research and Technology (GSRT) {[}09-SYN-23-879];
grant SEE-ERA. NET PLUS {[}ERA 64/01]; grant KRIPIS {[}MIS 448840] | |
dc.language | English | |
dc.relation | General Secretariat of Research and Technology (GSRT) grant 09-SYN-23-879, grant SEE-ERA. NET PLUS: ERA 64/01 and grant KRIPIS (grant no. MIS 448840) | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Bmc Genomics | |
dc.subject | Miltiradiene | |
dc.subject | S. pomifera | |
dc.subject | Diterpenes | |
dc.subject | Cytochrome P450 | |
dc.title | Combined metabolome and transcriptome profiling provides new insights
into diterpene biosynthesis in S. pomifera glandular trichomes | en |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Трикка, Фотини A.; Иоанноу, Ефстатхиа; Роуссис, Вассилиос; Тзивелека, Лето-Aикатерини; Тсабалла, Aпхродите; Стеа, Елени A.; Aргириоу, Aнагностис; Канеллис, Aнгелос К.; Кампранис, Сотириос Ц.; Макрис, Aнтониос М.; Божић, Драгана; Игнеа, Цодрута; Николаидис, Aлеxандрос; | |
dc.rights.holder | © 2015 Trikka et al | |
dc.citation.issue | 935 | |
dc.citation.volume | 16 | |
dc.identifier.doi | 10.1186/s12864-015-2147-3 | |
dc.identifier.scopus | 2-s2.0-84963593778 | |
dc.identifier.wos | 000365283800010 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/6082/document.pdf | |
dc.citation.rank | M21 | |