TY  - CONF
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Miljić, Dragana
AU  - Ćirić, Jasmina
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Köhrle, Josef
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6206
AB  - Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.
PB  - Bioscientifica
C3  - 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
T1  - Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats
DO  - 10.1530/endoabs.56.gp246
SP  - GP246
ER  - 

@conference{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Miljić, Dragana and Ćirić, Jasmina and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Köhrle, Josef and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.",
publisher = "Bioscientifica",
journal = "20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain",
title = "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats",
doi = "10.1530/endoabs.56.gp246",
pages = "GP246"
}

Šošić-Jurjević, B., Lütjohann, D., Miljić, D., Ćirić, J., Trifunović, S., Ajdžanović, V., Filipović, B., Köhrle, J., Ušćebrka, G.,& Milošević, V.. (2018). Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
Bioscientifica., GP246.
https://doi.org/10.1530/endoabs.56.gp246

Šošić-Jurjević B, Lütjohann D, Miljić D, Ćirić J, Trifunović S, Ajdžanović V, Filipović B, Köhrle J, Ušćebrka G, Milošević V. Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain. 2018;:GP246.
doi:10.1530/endoabs.56.gp246 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Miljić, Dragana, Ćirić, Jasmina, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Köhrle, Josef, Ušćebrka, Gordana, Milošević, Verica, "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats" in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain (2018):GP246,
https://doi.org/10.1530/endoabs.56.gp246 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Wirth, Eva Katrin
AU  - Živanović, Jasmina
AU  - Radulović, Niko
AU  - Janković, Snežana
AU  - Milošević, Verica
AU  - Köhrle, Josef
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6134
AB  - We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh beta, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T-3-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T-4 bound to Tg, determined thyroid T-4 levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T-4 levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats.
PB  - Elsevier Inc
T2  - Toxicology and Applied Pharmacology
T1  - Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats
IS  - 2
VL  - 278
DO  - 10.1016/j.taap.2014.04.018
SP  - 124
EP  - 134
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Filipović, Branko and Wirth, Eva Katrin and Živanović, Jasmina and Radulović, Niko and Janković, Snežana and Milošević, Verica and Köhrle, Josef",
year = "2014",
abstract = "We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh beta, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T-3-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T-4 bound to Tg, determined thyroid T-4 levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T-4 levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats.",
publisher = "Elsevier Inc",
journal = "Toxicology and Applied Pharmacology",
title = "Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats",
number = "2",
volume = "278",
doi = "10.1016/j.taap.2014.04.018",
pages = "124-134"
}

Šošić-Jurjević, B., Filipović, B., Wirth, E. K., Živanović, J., Radulović, N., Janković, S., Milošević, V.,& Köhrle, J.. (2014). Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. in Toxicology and Applied Pharmacology
Elsevier Inc., 278(2), 124-134.
https://doi.org/10.1016/j.taap.2014.04.018

Šošić-Jurjević B, Filipović B, Wirth EK, Živanović J, Radulović N, Janković S, Milošević V, Köhrle J. Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. in Toxicology and Applied Pharmacology. 2014;278(2):124-134.
doi:10.1016/j.taap.2014.04.018 .

Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, Köhrle, Josef, "Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats" in Toxicology and Applied Pharmacology, 278, no. 2 (2014):124-134,
https://doi.org/10.1016/j.taap.2014.04.018 . .