TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5117
AB  - The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.
PB  - Basel: MDPI
T2  - Life
T1  - Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose
IS  - 10
VL  - 12
DO  - 10.3390/life12101585
SP  - 1585
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Sajid, Sumbal and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose",
number = "10",
volume = "12",
doi = "10.3390/life12101585",
pages = "1585"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life
Basel: MDPI., 12(10), 1585.
https://doi.org/10.3390/life12101585

Ćosić T, Motyka V, Raspor M, Sajid S, Devrnja N, Dobrev PI, Ninković S. Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life. 2022;12(10):1585.
doi:10.3390/life12101585 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Sajid, Sumbal, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose" in Life, 12, no. 10 (2022):1585,
https://doi.org/10.3390/life12101585 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dobrev, Petre I.
AU  - Marković, Marija
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4649
AB  - Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
VL  - 11
DO  - 10.1038/s41598-021-00866-7
SP  - 21471
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dobrev, Petre I. and Marković, Marija and Milošević, Snežana and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro",
volume = "11",
doi = "10.1038/s41598-021-00866-7",
pages = "21471"
}

Trifunović-Momčilov, M., Motyka, V., Dobrev, P. I., Marković, M., Milošević, S., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports
Springer Nature., 11, 21471.
https://doi.org/10.1038/s41598-021-00866-7

Trifunović-Momčilov M, Motyka V, Dobrev PI, Marković M, Milošević S, Jevremović S, Dragićević IČ, Subotić A. Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports. 2021;11:21471.
doi:10.1038/s41598-021-00866-7 .

Trifunović-Momčilov, Milana, Motyka, Václav, Dobrev, Petre I., Marković, Marija, Milošević, Snežana, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro" in Scientific Reports, 11 (2021):21471,
https://doi.org/10.1038/s41598-021-00866-7 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Simonović, Ana
AU  - Motyka, Václav
AU  - Dobrev, Petre I
AU  - Budimir, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2989
UR  - https://link.springer.com/article/10.1007%2Fs00709-015-0802-9#aboutcontent
AB  - Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.
PB  - Springer-Verlag Wien
T2  - Protoplasma
T1  - Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.
IS  - 2
VL  - 253
DO  - 10.1007/s00709-015-0802-9
SP  - 259
EP  - 75
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Simonović, Ana and Motyka, Václav and Dobrev, Petre I and Budimir, Snežana",
year = "2016",
abstract = "Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.",
publisher = "Springer-Verlag Wien",
journal = "Protoplasma",
title = "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.",
number = "2",
volume = "253",
doi = "10.1007/s00709-015-0802-9",
pages = "259-75"
}

Uzelac, B., Janošević, D., Simonović, A., Motyka, V., Dobrev, P. I.,& Budimir, S.. (2016). Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma
Springer-Verlag Wien., 253(2), 259-75.
https://doi.org/10.1007/s00709-015-0802-9

Uzelac B, Janošević D, Simonović A, Motyka V, Dobrev PI, Budimir S. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma. 2016;253(2):259-75.
doi:10.1007/s00709-015-0802-9 .

Uzelac, Branka, Janošević, Dušica, Simonović, Ana, Motyka, Václav, Dobrev, Petre I, Budimir, Snežana, "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro." in Protoplasma, 253, no. 2 (2016):259-75,
https://doi.org/10.1007/s00709-015-0802-9 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dragićević, Ivana C.
AU  - Petrić, Marija
AU  - Jevremović, Slađana
AU  - Malbeck, Jiří
AU  - Holik, Josef
AU  - Dobrev, Petre I.
AU  - Subotić, Angelina
PY  - 2016
UR  - https://link.springer.com/article/10.1007%2Fs00344-015-9558-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3381
AB  - Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.
T2  - Journal of Plant Growth Regulation
T1  - Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro
IS  - 2
VL  - 35
DO  - 10.1007/s00344-015-9558-x
SP  - 543
EP  - 552
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dragićević, Ivana C. and Petrić, Marija and Jevremović, Slađana and Malbeck, Jiří and Holik, Josef and Dobrev, Petre I. and Subotić, Angelina",
year = "2016",
abstract = "Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.",
journal = "Journal of Plant Growth Regulation",
title = "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro",
number = "2",
volume = "35",
doi = "10.1007/s00344-015-9558-x",
pages = "543-552"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I. C., Petrić, M., Jevremović, S., Malbeck, J., Holik, J., Dobrev, P. I.,& Subotić, A.. (2016). Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation, 35(2), 543-552.
https://doi.org/10.1007/s00344-015-9558-x

Trifunović-Momčilov M, Motyka V, Dragićević IC, Petrić M, Jevremović S, Malbeck J, Holik J, Dobrev PI, Subotić A. Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation. 2016;35(2):543-552.
doi:10.1007/s00344-015-9558-x .

Trifunović-Momčilov, Milana, Motyka, Václav, Dragićević, Ivana C., Petrić, Marija, Jevremović, Slađana, Malbeck, Jiří, Holik, Josef, Dobrev, Petre I., Subotić, Angelina, "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro" in Journal of Plant Growth Regulation, 35, no. 2 (2016):543-552,
https://doi.org/10.1007/s00344-015-9558-x . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Petrić, Marija
AU  - Holik, Josef
AU  - Malbeck, Jiri
AU  - Dobrev, Petre I.
AU  - Dragicevic, Ivana C.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2012
AB  - The plant hormones cytokinins (CKs) regulate a number of physiological
   processes. Their homeostasis is controlled by the rate of de novo
   synthesis and the rate of catabolism. The aim of this work was to
   analyze the content of total as well as individual groups of endogenous
   CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea
   Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable
   model system for studying physiological and morphological processes
   controlled by CKs. In this work we clearly demonstrate a significant
   effect of AtCKX transgenes on CK metabolism in transgenic centaury
   plants. However, shoots and roots of only one AtCKX1 line and three
   AtCKX2 lines with a significant reduction of bioactive CKs were
   obtained. We also show that changes in the CKs metabolism considerably
   affected endogenous indole-3-acetic acid (IAA) levels in plant tissues.
   All analyzed transgenic AtCKX centaury lines exhibited decreased amount
   of endogenous IAA in shoots as well as in roots. Consequently, the
   IAA/bioactive CK forms ratios showed a significant variation in the
   shoots and roots of all analyzed AtCKX centaury transformants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro
IS  - 2
VL  - 120
DO  - 10.1007/s11240-014-0646-6
SP  - 767
EP  - 777
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana and Petrić, Marija and Holik, Josef and Malbeck, Jiri and Dobrev, Petre I. and Dragicevic, Ivana C.",
year = "2015",
abstract = "The plant hormones cytokinins (CKs) regulate a number of physiological
   processes. Their homeostasis is controlled by the rate of de novo
   synthesis and the rate of catabolism. The aim of this work was to
   analyze the content of total as well as individual groups of endogenous
   CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea
   Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable
   model system for studying physiological and morphological processes
   controlled by CKs. In this work we clearly demonstrate a significant
   effect of AtCKX transgenes on CK metabolism in transgenic centaury
   plants. However, shoots and roots of only one AtCKX1 line and three
   AtCKX2 lines with a significant reduction of bioactive CKs were
   obtained. We also show that changes in the CKs metabolism considerably
   affected endogenous indole-3-acetic acid (IAA) levels in plant tissues.
   All analyzed transgenic AtCKX centaury lines exhibited decreased amount
   of endogenous IAA in shoots as well as in roots. Consequently, the
   IAA/bioactive CK forms ratios showed a significant variation in the
   shoots and roots of all analyzed AtCKX centaury transformants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro",
number = "2",
volume = "120",
doi = "10.1007/s11240-014-0646-6",
pages = "767-777"
}

Trifunović-Momčilov, M., Motyka, V., Cingel, A., Subotić, A., Jevremović, S., Petrić, M., Holik, J., Malbeck, J., Dobrev, P. I.,& Dragicevic, I. C.. (2015). Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro. in Plant Cell Tissue and Organ Culture, 120(2), 767-777.
https://doi.org/10.1007/s11240-014-0646-6

Trifunović-Momčilov M, Motyka V, Cingel A, Subotić A, Jevremović S, Petrić M, Holik J, Malbeck J, Dobrev PI, Dragicevic IC. Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro. in Plant Cell Tissue and Organ Culture. 2015;120(2):767-777.
doi:10.1007/s11240-014-0646-6 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, Petrić, Marija, Holik, Josef, Malbeck, Jiri, Dobrev, Petre I., Dragicevic, Ivana C., "Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro" in Plant Cell Tissue and Organ Culture, 120, no. 2 (2015):767-777,
https://doi.org/10.1007/s11240-014-0646-6 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Travnickova, Alena
AU  - Dobrev, Petre I.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1956
AB  - Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.
T2  - Plant Cell Tissue and Organ Culture
T1  - In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0742-2
SP  - 741
EP  - 760
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Savic, Jelena and Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Travnickova, Alena and Dobrev, Petre I. and Bohanec, Borut and Ninković, Slavica",
year = "2015",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.",
journal = "Plant Cell Tissue and Organ Culture",
title = "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0742-2",
pages = "741-760"
}

Ćosić, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnickova, A., Dobrev, P. I., Bohanec, B.,& Ninković, S.. (2015). In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture, 121(3), 741-760.
https://doi.org/10.1007/s11240-015-0742-2

Ćosić T, Motyka V, Raspor M, Savic J, Cingel A, Vinterhalter B, Vinterhalter D, Travnickova A, Dobrev PI, Bohanec B, Ninković S. In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture. 2015;121(3):741-760.
doi:10.1007/s11240-015-0742-2 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Savic, Jelena, Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Travnickova, Alena, Dobrev, Petre I., Bohanec, Borut, Ninković, Slavica, "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):741-760,
https://doi.org/10.1007/s11240-015-0742-2 . .