TY  - JOUR
AU  - Bjekić-Macut, Jelica
AU  - Vukašin, Tamara
AU  - Velija-Ašimi, Zelija
AU  - Bureković, Azra
AU  - Zdravković, Marija
AU  - Andrić, Zoran
AU  - Branković, Marija
AU  - Crevar-Marinović, Slobodanka
AU  - Mandić, Tatjana
AU  - Stanojlović, Olivera
AU  - Vojnović-Milutinović, Danijela
AU  - Livadas, Sarantis
AU  - Mastorakos, George
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4307
AB  - Polycystic ovary syndrome (PCOS) is a frequent endocrine disease in women during reproductive period. It is considered a complex metabolic disorder with long-term metabolic, as well as reproductive consequences. Main pathophysiological pathways are related to the increased androgen levels and insulin resistance. Nowadays, genetic origins of PCOS are acknowledged, with numerous genes involved in the pathogenesis of hyperandrogenemia, insulin resistance, inflammation and disturbed folliculogenesis. Rotterdam diagnostic criteria are most widely accepted and four PCOS phenotypes have been recognized. Metabolic abnormalities are more common in phenotypes 1 and 2. Women with classic PCOS are more obese and typically have central type of obesity, more prevalently displaying dyslipidemia, insulin resistance and metabolic syndrome that could be associated with an increased risk of cardiovascular complications during life. Heterogeneity of phenotypes demands an individualized approach in the treatment of women with PCOS. Metabolic therapies involve a lifestyle intervention followed by the introduction of insulin sensitizers including metformin and inositols, glucagon-like peptide 1 receptor agonists (GLP-1 RA), as recently sodium glucose contransporter-2 (SGLT2) inhibitors. Addition of an insulin sensitizer to the standard infertility therapy such as CC improves ovulation and pregnancy rates. Our current review analyzes the contemporary knowledge of PCOS etiology and etiopathogenesis, its cardiometabolic risks and their outcomes, as well as therapeutic advances for women with PCOS.
PB  - Bentham Science Publishers
T2  - Current Pharmaceutical Design
T1  - Polycystic Ovary Syndrome: A Contemporary Clinical Approach
VL  - 27
DO  - 10.2174/1381612827666210119104721
SP  - 1
EP  - 9
ER  - 

@article{
author = "Bjekić-Macut, Jelica and Vukašin, Tamara and Velija-Ašimi, Zelija and Bureković, Azra and Zdravković, Marija and Andrić, Zoran and Branković, Marija and Crevar-Marinović, Slobodanka and Mandić, Tatjana and Stanojlović, Olivera and Vojnović-Milutinović, Danijela and Livadas, Sarantis and Mastorakos, George",
year = "2021",
abstract = "Polycystic ovary syndrome (PCOS) is a frequent endocrine disease in women during reproductive period. It is considered a complex metabolic disorder with long-term metabolic, as well as reproductive consequences. Main pathophysiological pathways are related to the increased androgen levels and insulin resistance. Nowadays, genetic origins of PCOS are acknowledged, with numerous genes involved in the pathogenesis of hyperandrogenemia, insulin resistance, inflammation and disturbed folliculogenesis. Rotterdam diagnostic criteria are most widely accepted and four PCOS phenotypes have been recognized. Metabolic abnormalities are more common in phenotypes 1 and 2. Women with classic PCOS are more obese and typically have central type of obesity, more prevalently displaying dyslipidemia, insulin resistance and metabolic syndrome that could be associated with an increased risk of cardiovascular complications during life. Heterogeneity of phenotypes demands an individualized approach in the treatment of women with PCOS. Metabolic therapies involve a lifestyle intervention followed by the introduction of insulin sensitizers including metformin and inositols, glucagon-like peptide 1 receptor agonists (GLP-1 RA), as recently sodium glucose contransporter-2 (SGLT2) inhibitors. Addition of an insulin sensitizer to the standard infertility therapy such as CC improves ovulation and pregnancy rates. Our current review analyzes the contemporary knowledge of PCOS etiology and etiopathogenesis, its cardiometabolic risks and their outcomes, as well as therapeutic advances for women with PCOS.",
publisher = "Bentham Science Publishers",
journal = "Current Pharmaceutical Design",
title = "Polycystic Ovary Syndrome: A Contemporary Clinical Approach",
volume = "27",
doi = "10.2174/1381612827666210119104721",
pages = "1-9"
}

Bjekić-Macut, J., Vukašin, T., Velija-Ašimi, Z., Bureković, A., Zdravković, M., Andrić, Z., Branković, M., Crevar-Marinović, S., Mandić, T., Stanojlović, O., Vojnović-Milutinović, D., Livadas, S.,& Mastorakos, G.. (2021). Polycystic Ovary Syndrome: A Contemporary Clinical Approach. in Current Pharmaceutical Design
Bentham Science Publishers., 27, 1-9.
https://doi.org/10.2174/1381612827666210119104721

Bjekić-Macut J, Vukašin T, Velija-Ašimi Z, Bureković A, Zdravković M, Andrić Z, Branković M, Crevar-Marinović S, Mandić T, Stanojlović O, Vojnović-Milutinović D, Livadas S, Mastorakos G. Polycystic Ovary Syndrome: A Contemporary Clinical Approach. in Current Pharmaceutical Design. 2021;27:1-9.
doi:10.2174/1381612827666210119104721 .

Bjekić-Macut, Jelica, Vukašin, Tamara, Velija-Ašimi, Zelija, Bureković, Azra, Zdravković, Marija, Andrić, Zoran, Branković, Marija, Crevar-Marinović, Slobodanka, Mandić, Tatjana, Stanojlović, Olivera, Vojnović-Milutinović, Danijela, Livadas, Sarantis, Mastorakos, George, "Polycystic Ovary Syndrome: A Contemporary Clinical Approach" in Current Pharmaceutical Design, 27 (2021):1-9,
https://doi.org/10.2174/1381612827666210119104721 . .

TY  - JOUR
AU  - Vojnović Milutinović, Danijela
AU  - Radovanović, Marina
AU  - Veličković, Nataša
AU  - Đorđević, Ana
AU  - Bursać, Biljana
AU  - Brkljačić, Jelena
AU  - Teofilović, Ana
AU  - Božić Antić, Ivana
AU  - Bjekić-Macut, Jelica
AU  - Shirif Zidane, Abdulbaset
AU  - Matić, Gordana
AU  - Macut, Đuro
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6331
AB  - Polycystic ovary syndrome is a heterogeneous endocrine and metabolic disorder associated with abdominal obesity, dyslipidemia and insulin resistance. Since abdominal obesity is characterized by low-grade inflammation, the aim of the study was to investigate whether visceral adipose tissue inflammation linked to abdominal obesity and dyslipidemia could lead to impaired insulin sensitivity in the animal model of polycystic ovary syndrome. 
Female Wistar rats were treated with nonaromatizable 5α dihydrotestosterone pellets in order to induce reproductive and metabolic characteristics of polycystic ovary syndrome. Glucose, triglycerides, non-esterified fatty acids and insulin were determined in blood plasma. Visceral adipose tissue inflammation was evaluated by the nuclear factor kappa B intracellular distribution, macrophage migration inhibitory factor protein level, as well as TNFα, IL6 and IL1β mRNA levels. Insulin sensitivity was assessed by intraperitoneal glucose tolerance test and homeostasis model assessment index, and through analysis of insulin signaling pathway in the visceral adipose tissue.
Dihydrotestosterone treatment led to increased body weight, abdominal obesity and elevated triglycerides and non-esterified fatty acids, which were accompanied by the activation of nuclear factor kappa B and increase in macrophage migration inhibitory factor, IL6 and IL1β levels in the visceral adipose tissue. In parallel, insulin sensitivity was affected in 5α dihydrotestosterone-treated animals only at the systemic, and not at the level of visceral adipose tissue. 
The results showed that abdominal obesity and dyslipidemia in the animal model of polycystic ovary syndrome were accompanied with low-grade inflammation in the visceral adipose tissue. However, these metabolic disturbances did not result in decreased tissue insulin sensitivity.
PB  - Stuttgart: Georg Thieme Verlag KG
T2  - Experimental and Clinical Endocrinology and Diabetes
T1  - Enhanced inflammation without impairment of insulin signaling in the visceral adipose tissue of 5α- dihydrotestosterone-induced animal model of polycystic ovary syndrome
IS  - 8
VL  - 125
DO  - 10.1055/s-0043-104531
SP  - 522
EP  - 529
ER  - 

@article{
author = "Vojnović Milutinović, Danijela and Radovanović, Marina and Veličković, Nataša and Đorđević, Ana and Bursać, Biljana and Brkljačić, Jelena and Teofilović, Ana and Božić Antić, Ivana and Bjekić-Macut, Jelica and Shirif Zidane, Abdulbaset and Matić, Gordana and Macut, Đuro",
year = "2017",
abstract = "Polycystic ovary syndrome is a heterogeneous endocrine and metabolic disorder associated with abdominal obesity, dyslipidemia and insulin resistance. Since abdominal obesity is characterized by low-grade inflammation, the aim of the study was to investigate whether visceral adipose tissue inflammation linked to abdominal obesity and dyslipidemia could lead to impaired insulin sensitivity in the animal model of polycystic ovary syndrome. 
Female Wistar rats were treated with nonaromatizable 5α dihydrotestosterone pellets in order to induce reproductive and metabolic characteristics of polycystic ovary syndrome. Glucose, triglycerides, non-esterified fatty acids and insulin were determined in blood plasma. Visceral adipose tissue inflammation was evaluated by the nuclear factor kappa B intracellular distribution, macrophage migration inhibitory factor protein level, as well as TNFα, IL6 and IL1β mRNA levels. Insulin sensitivity was assessed by intraperitoneal glucose tolerance test and homeostasis model assessment index, and through analysis of insulin signaling pathway in the visceral adipose tissue.
Dihydrotestosterone treatment led to increased body weight, abdominal obesity and elevated triglycerides and non-esterified fatty acids, which were accompanied by the activation of nuclear factor kappa B and increase in macrophage migration inhibitory factor, IL6 and IL1β levels in the visceral adipose tissue. In parallel, insulin sensitivity was affected in 5α dihydrotestosterone-treated animals only at the systemic, and not at the level of visceral adipose tissue. 
The results showed that abdominal obesity and dyslipidemia in the animal model of polycystic ovary syndrome were accompanied with low-grade inflammation in the visceral adipose tissue. However, these metabolic disturbances did not result in decreased tissue insulin sensitivity.",
publisher = "Stuttgart: Georg Thieme Verlag KG",
journal = "Experimental and Clinical Endocrinology and Diabetes",
title = "Enhanced inflammation without impairment of insulin signaling in the visceral adipose tissue of 5α- dihydrotestosterone-induced animal model of polycystic ovary syndrome",
number = "8",
volume = "125",
doi = "10.1055/s-0043-104531",
pages = "522-529"
}

Vojnović Milutinović, D., Radovanović, M., Veličković, N., Đorđević, A., Bursać, B., Brkljačić, J., Teofilović, A., Božić Antić, I., Bjekić-Macut, J., Shirif Zidane, A., Matić, G.,& Macut, Đ.. (2017). Enhanced inflammation without impairment of insulin signaling in the visceral adipose tissue of 5α- dihydrotestosterone-induced animal model of polycystic ovary syndrome. in Experimental and Clinical Endocrinology and Diabetes
Stuttgart: Georg Thieme Verlag KG., 125(8), 522-529.
https://doi.org/10.1055/s-0043-104531

Vojnović Milutinović D, Radovanović M, Veličković N, Đorđević A, Bursać B, Brkljačić J, Teofilović A, Božić Antić I, Bjekić-Macut J, Shirif Zidane A, Matić G, Macut Đ. Enhanced inflammation without impairment of insulin signaling in the visceral adipose tissue of 5α- dihydrotestosterone-induced animal model of polycystic ovary syndrome. in Experimental and Clinical Endocrinology and Diabetes. 2017;125(8):522-529.
doi:10.1055/s-0043-104531 .

Vojnović Milutinović, Danijela, Radovanović, Marina, Veličković, Nataša, Đorđević, Ana, Bursać, Biljana, Brkljačić, Jelena, Teofilović, Ana, Božić Antić, Ivana, Bjekić-Macut, Jelica, Shirif Zidane, Abdulbaset, Matić, Gordana, Macut, Đuro, "Enhanced inflammation without impairment of insulin signaling in the visceral adipose tissue of 5α- dihydrotestosterone-induced animal model of polycystic ovary syndrome" in Experimental and Clinical Endocrinology and Diabetes, 125, no. 8 (2017):522-529,
https://doi.org/10.1055/s-0043-104531 . .

TY  - JOUR
AU  - Radovanović, Marina
AU  - Macut, Đuro
AU  - Đorđević, Ana
AU  - Veličković, Nataša
AU  - Nestorović, Nataša
AU  - Bursać, Biljana
AU  - Božić-Antić, Ivana
AU  - Bjekić Macut, Jelica
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2022
UR  - https://www.sciencedirect.com/science/article/pii/S0303720714002706?via%3Dihub#ac0010
AB  - Polycystic ovary syndrome (PCOS) is a reproductive and metabolic
   disorder characterized by hyperandrogenism, ovulatory dysfunction,
   visceral obesity and insulin resistance. We hypothesized that changes in
   glucocorticoid metabolism and signaling in the visceral adipose tissue
   may contribute to disturbances of lipid metabolism in the rat model of
   PCOS obtained by 5 alpha-dihydrotestosterone (DHT) treatment of
   prepubertal female Wistar rats. The results confirmed that DHT treatment
   caused anovulation, obesity and dyslipidemia. Enhanced glucocorticoid
   prereceptor metabolism, assessed by elevated intracellular
   corticosterone and increased 11 beta-hydroxysteroid dehydrogenase type 1
   mRNA and protein levels, was accompanied by glucocorticoid receptor (GR)
   nuclear accumulation. In concert with the increased expression of
   GR-regulated prolipogenic genes (lipin-1, sterol regulatory element
   binding protein 1, fatty acid synthase, phosphoenolpyruvate
   carboxykinase), histological analyses revealed hypertrophic adipocytes.
   The results suggest that glucocorticoids influence lipid metabolism in
   the visceral adipose tissue in the way that may contribute to
   pathogenesis of metabolic disturbances associated with PCOS. (C) 2014
   Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue
IS  - C
VL  - 399
DO  - 10.1016/j.mce.2014.08.013
SP  - 22
EP  - 31
ER  - 

@article{
author = "Radovanović, Marina and Macut, Đuro and Đorđević, Ana and Veličković, Nataša and Nestorović, Nataša and Bursać, Biljana and Božić-Antić, Ivana and Bjekić Macut, Jelica and Matić, Gordana and Vojnović-Milutinović, Danijela",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is a reproductive and metabolic
   disorder characterized by hyperandrogenism, ovulatory dysfunction,
   visceral obesity and insulin resistance. We hypothesized that changes in
   glucocorticoid metabolism and signaling in the visceral adipose tissue
   may contribute to disturbances of lipid metabolism in the rat model of
   PCOS obtained by 5 alpha-dihydrotestosterone (DHT) treatment of
   prepubertal female Wistar rats. The results confirmed that DHT treatment
   caused anovulation, obesity and dyslipidemia. Enhanced glucocorticoid
   prereceptor metabolism, assessed by elevated intracellular
   corticosterone and increased 11 beta-hydroxysteroid dehydrogenase type 1
   mRNA and protein levels, was accompanied by glucocorticoid receptor (GR)
   nuclear accumulation. In concert with the increased expression of
   GR-regulated prolipogenic genes (lipin-1, sterol regulatory element
   binding protein 1, fatty acid synthase, phosphoenolpyruvate
   carboxykinase), histological analyses revealed hypertrophic adipocytes.
   The results suggest that glucocorticoids influence lipid metabolism in
   the visceral adipose tissue in the way that may contribute to
   pathogenesis of metabolic disturbances associated with PCOS. (C) 2014
   Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue",
number = "C",
volume = "399",
doi = "10.1016/j.mce.2014.08.013",
pages = "22-31"
}

Radovanović, M., Macut, Đ., Đorđević, A., Veličković, N., Nestorović, N., Bursać, B., Božić-Antić, I., Bjekić Macut, J., Matić, G.,& Vojnović-Milutinović, D.. (2015). Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue. in Molecular and Cellular Endocrinology, 399(C), 22-31.
https://doi.org/10.1016/j.mce.2014.08.013

Radovanović M, Macut Đ, Đorđević A, Veličković N, Nestorović N, Bursać B, Božić-Antić I, Bjekić Macut J, Matić G, Vojnović-Milutinović D. Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue. in Molecular and Cellular Endocrinology. 2015;399(C):22-31.
doi:10.1016/j.mce.2014.08.013 .

Radovanović, Marina, Macut, Đuro, Đorđević, Ana, Veličković, Nataša, Nestorović, Nataša, Bursać, Biljana, Božić-Antić, Ivana, Bjekić Macut, Jelica, Matić, Gordana, Vojnović-Milutinović, Danijela, "Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue" in Molecular and Cellular Endocrinology, 399, no. C (2015):22-31,
https://doi.org/10.1016/j.mce.2014.08.013 . .

TY  - JOUR
AU  - Macut, Djuro
AU  - Bozic-Antic, Ivana
AU  - Brkljačić, Jelena
AU  - Topalovic, Vladanka
AU  - Macut, Jelica Bjekic
AU  - Panidis, Dimitrios
AU  - Kotlica, Biljana Kastratovic
AU  - Papadakis, Efstathios
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2069
AB  - OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.
T2  - Hormones-International Journal of Endocrinology and Metabolism
T1  - The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome
IS  - 1
VL  - 14
SP  - 109
EP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2069
ER  - 

@article{
author = "Macut, Djuro and Bozic-Antic, Ivana and Brkljačić, Jelena and Topalovic, Vladanka and Macut, Jelica Bjekic and Panidis, Dimitrios and Kotlica, Biljana Kastratovic and Papadakis, Efstathios and Matić, Gordana and Vojnović-Milutinović, Danijela",
year = "2015",
abstract = "OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.",
journal = "Hormones-International Journal of Endocrinology and Metabolism",
title = "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome",
number = "1",
volume = "14",
pages = "109-117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2069"
}

Macut, D., Bozic-Antic, I., Brkljačić, J., Topalovic, V., Macut, J. B., Panidis, D., Kotlica, B. K., Papadakis, E., Matić, G.,& Vojnović-Milutinović, D.. (2015). The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism, 14(1), 109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069

Macut D, Bozic-Antic I, Brkljačić J, Topalovic V, Macut JB, Panidis D, Kotlica BK, Papadakis E, Matić G, Vojnović-Milutinović D. The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism. 2015;14(1):109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .

Macut, Djuro, Bozic-Antic, Ivana, Brkljačić, Jelena, Topalovic, Vladanka, Macut, Jelica Bjekic, Panidis, Dimitrios, Kotlica, Biljana Kastratovic, Papadakis, Efstathios, Matić, Gordana, Vojnović-Milutinović, Danijela, "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome" in Hormones-International Journal of Endocrinology and Metabolism, 14, no. 1 (2015):109-117,
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .

TY  - JOUR
AU  - Vojnović-Milutinović, Danijela
AU  - Macut, Djuro
AU  - Bozic-Antic, Ivana
AU  - Macut, Jelica Bjekic
AU  - Radovanović, Marina
AU  - Matić, Gordana
AU  - Brkljačić, Jelena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2157
AB  - Background: The polycystic ovary syndrome (PCOS) is a frequent endocrine
   disorder that affects women of reproductive age. As the syndrome is
   strongly associated with obesity, it is of interest to examine the gene
   expression differences that accompany its development and the associated
   metabolic disturbances. Real-time RT PCR is a standard method for
   studying changes in gene expression. However, to obtain accurate and
   reliable results, validation of reference genes is obligatory. The aim
   of this study was to identify a suitable reference for the normalization
   of gene expression in peripheral blood mononuclear cells (PBMCs) from
   obese and normal-weight women with PCOS.
   Methods: The expression stability of four potential reference genes:
   hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), beta-actin
   (BA), beta(2)-microglobulin (B2M) and glyceraldehyde-3-phosphate
   dehydrogenase (GAPDH), was assessed in PBMCs from healthy women, and
   from normal-weight and obese women with PCOS. The variability in the
   expression of potential reference genes was analyzed by the TaqMan
   real-time RT PCR method, using GeNorm and Norm Finder software packages.
   Results: Direct comparison of cycle threshold (Ct) values showed
   inter-individual variations for all validated genes, the Ct values of
   HPRT being less variable than those of BA, GAPDH and B2M. Both software
   packages pointed to HPRT as the most steadily expressed gene in the
   PBMCs of women with PCOS and healthy controls.
   Conclusions: Cross-validation of the expression stability of four
   potential reference genes identified HPRT as the most stable reference,
   suitable for further investigations of gene expression in PBMCs from
   women with PCOS.
T2  - Journal of Medical Biochemistry
T1  - Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome
IS  - 4
VL  - 33
DO  - 10.2478/jomb-2014-0029
SP  - 356
EP  - 363
ER  - 

@article{
author = "Vojnović-Milutinović, Danijela and Macut, Djuro and Bozic-Antic, Ivana and Macut, Jelica Bjekic and Radovanović, Marina and Matić, Gordana and Brkljačić, Jelena",
year = "2014",
abstract = "Background: The polycystic ovary syndrome (PCOS) is a frequent endocrine
   disorder that affects women of reproductive age. As the syndrome is
   strongly associated with obesity, it is of interest to examine the gene
   expression differences that accompany its development and the associated
   metabolic disturbances. Real-time RT PCR is a standard method for
   studying changes in gene expression. However, to obtain accurate and
   reliable results, validation of reference genes is obligatory. The aim
   of this study was to identify a suitable reference for the normalization
   of gene expression in peripheral blood mononuclear cells (PBMCs) from
   obese and normal-weight women with PCOS.
   Methods: The expression stability of four potential reference genes:
   hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), beta-actin
   (BA), beta(2)-microglobulin (B2M) and glyceraldehyde-3-phosphate
   dehydrogenase (GAPDH), was assessed in PBMCs from healthy women, and
   from normal-weight and obese women with PCOS. The variability in the
   expression of potential reference genes was analyzed by the TaqMan
   real-time RT PCR method, using GeNorm and Norm Finder software packages.
   Results: Direct comparison of cycle threshold (Ct) values showed
   inter-individual variations for all validated genes, the Ct values of
   HPRT being less variable than those of BA, GAPDH and B2M. Both software
   packages pointed to HPRT as the most steadily expressed gene in the
   PBMCs of women with PCOS and healthy controls.
   Conclusions: Cross-validation of the expression stability of four
   potential reference genes identified HPRT as the most stable reference,
   suitable for further investigations of gene expression in PBMCs from
   women with PCOS.",
journal = "Journal of Medical Biochemistry",
title = "Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome",
number = "4",
volume = "33",
doi = "10.2478/jomb-2014-0029",
pages = "356-363"
}

Vojnović-Milutinović, D., Macut, D., Bozic-Antic, I., Macut, J. B., Radovanović, M., Matić, G.,& Brkljačić, J.. (2014). Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome. in Journal of Medical Biochemistry, 33(4), 356-363.
https://doi.org/10.2478/jomb-2014-0029

Vojnović-Milutinović D, Macut D, Bozic-Antic I, Macut JB, Radovanović M, Matić G, Brkljačić J. Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome. in Journal of Medical Biochemistry. 2014;33(4):356-363.
doi:10.2478/jomb-2014-0029 .

Vojnović-Milutinović, Danijela, Macut, Djuro, Bozic-Antic, Ivana, Macut, Jelica Bjekic, Radovanović, Marina, Matić, Gordana, Brkljačić, Jelena, "Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome" in Journal of Medical Biochemistry, 33, no. 4 (2014):356-363,
https://doi.org/10.2478/jomb-2014-0029 . .