TY  - JOUR
AU  - Ilijin, Larisa
AU  - Nikolić, Maria Vesna
AU  - Vasiljević, Zorka
AU  - Todorović, Dajana
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Tadić, Nenad
AU  - Perić- Mataruga, Vesna
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6698
AB  - In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap
IS  - Part 2
VL  - 268
DO  - 10.1016/j.ijbiomac.2024.131731
SP  - 131731
ER  - 

@article{
author = "Ilijin, Larisa and Nikolić, Maria Vesna and Vasiljević, Zorka and Todorović, Dajana and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Tadić, Nenad and Perić- Mataruga, Vesna",
year = "2024",
abstract = "In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap",
number = "Part 2",
volume = "268",
doi = "10.1016/j.ijbiomac.2024.131731",
pages = "131731"
}

Ilijin, L., Nikolić, M. V., Vasiljević, Z., Todorović, D., Mrdaković, M., Vlahović, M., Matić, D., Tadić, N.,& Perić- Mataruga, V.. (2024). Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules
Elsevier., 268(Part 2), 131731.
https://doi.org/10.1016/j.ijbiomac.2024.131731

Ilijin L, Nikolić MV, Vasiljević Z, Todorović D, Mrdaković M, Vlahović M, Matić D, Tadić N, Perić- Mataruga V. Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules. 2024;268(Part 2):131731.
doi:10.1016/j.ijbiomac.2024.131731 .

Ilijin, Larisa, Nikolić, Maria Vesna, Vasiljević, Zorka, Todorović, Dajana, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Tadić, Nenad, Perić- Mataruga, Vesna, "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap" in International Journal of Biological Macromolecules, 268, no. Part 2 (2024):131731,
https://doi.org/10.1016/j.ijbiomac.2024.131731 . .

TY  - CONF
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Mirčić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6524
AB  - Aluminum (Al) is the most abundant metal in the Earth’s crust. It has a variety of applications in many
branches of industry, medicine, pharmacy and cosmetics. The mechanisms of toxicity of this metal
have not yet been elucidated. So far, the effects of pesticide containing Al have been investigated, while
the results describing the effect of different concentrations of this metal on phytophagous insects are
rare, especially in Serbia. In our experiment, larvae of gypsy moth (Lymantria dispar L., Erebidae) were
exposed to a chronic effect of dietary aluminum (treatment, T) (T1=50; T2 =250, T3=500 and T4=1000
microg/g of dry food), from hatching until the third day of the fifth larval stage (V3). Fitness traits
(relative growth rate – RGR, larval mass at V3, and development time – DT) were monitored, as the first
indicators of aluminum influence on growth and development.
Statistically significant differences were detected in all fitness traits between T2 and T4. In more detail,
differences were noticed between: Control – and treatments (T1 and T2) for larval mass and DT; Control
– and treatments (T3 and T4) for RGR and DT; as well as between T1 and T4 for RGR and larval mass. On
treatments T1, T2, T3, there are significant positive correlations between larval mass and RGR, while on
T2, T3 and T4, there are significant negative correlations between RGR and DT. Changes in insect fitness
traits can be the first warning signal of the aluminum presence in food.
AB  - Aluminijum (Al) je najzastupljeniji metal u Zemljinoj kori. Ovaj metal ima različite primene u mnogim
granama industrije, uključujući hranu, medicinu, farmaciju i kozmetiku. Mehanizmi toksičnosti ovog
metala još uvek nisu dovoljno razjašnjeni. Do sada su najčešće ispitivani efekti pesticida koji sadrže
aluminijum, dok su rezultati koji opisuju dejstvo različitih koncentracija ovog metala na fitofagne
insekte retki, posebno u Srbiji. U našem eksperimentu, larve gubara (Limantria dispar L., Erebidae)
bile su izložene hroničnom dejstvu aluminijuma (tretman, T) u hrani (T1=50; T2 =250, T3=500 i T4=1000
microg/g suve hrane), od izleganja do trećeg dana petog larvenog stupnja (V3). Praćene su osobine
fitnesa (relativna brzina rasta - RGR, masa larve (V3) i vreme razvića - DT), kao prvi pokazatelji uticaja
aluminijuma na rast i razvoj.
Statistički značajna razlika je otkrivena kod svih osobina fitnesa na T2 i T4. Primećene su značajne razlike:
između kontrole - i tretmana (T1 i T2) za masu larve i DT; kontrole - i tretmana (T3 i T4) za RGR i DT; kao
i između T1 i T4 za RGR i masu larve. Na tretmanima T1, T2, T3 postoje značajne pozitivne korelacije
između mase larvi i RGR, dok na T2, T3 i T4 postoje značajne negativne korelacije između RGR i DT.
Promene osobina fitnesa insekata mogu biti prvi signal upozorenja na prisustvo aluminijuma u hrani
PB  - Belgrade: Serbian Society of Toxicology
C3  - Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
T1  - Chronic effect of dietary aluminum on fitness traits in Lymantria dispar L. (Erebidae)
T1  - Hronični efekat aluminijuma u hrani na osobine fitnesa kod larvi Lymantria dispar L. (Erebidae)
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6524
ER  - 

@conference{
author = "Vlahović, Milena and Matić, Dragana and Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Mirčić, Dejan and Perić Mataruga, Vesna",
year = "2023",
abstract = "Aluminum (Al) is the most abundant metal in the Earth’s crust. It has a variety of applications in many
branches of industry, medicine, pharmacy and cosmetics. The mechanisms of toxicity of this metal
have not yet been elucidated. So far, the effects of pesticide containing Al have been investigated, while
the results describing the effect of different concentrations of this metal on phytophagous insects are
rare, especially in Serbia. In our experiment, larvae of gypsy moth (Lymantria dispar L., Erebidae) were
exposed to a chronic effect of dietary aluminum (treatment, T) (T1=50; T2 =250, T3=500 and T4=1000
microg/g of dry food), from hatching until the third day of the fifth larval stage (V3). Fitness traits
(relative growth rate – RGR, larval mass at V3, and development time – DT) were monitored, as the first
indicators of aluminum influence on growth and development.
Statistically significant differences were detected in all fitness traits between T2 and T4. In more detail,
differences were noticed between: Control – and treatments (T1 and T2) for larval mass and DT; Control
– and treatments (T3 and T4) for RGR and DT; as well as between T1 and T4 for RGR and larval mass. On
treatments T1, T2, T3, there are significant positive correlations between larval mass and RGR, while on
T2, T3 and T4, there are significant negative correlations between RGR and DT. Changes in insect fitness
traits can be the first warning signal of the aluminum presence in food., Aluminijum (Al) je najzastupljeniji metal u Zemljinoj kori. Ovaj metal ima različite primene u mnogim
granama industrije, uključujući hranu, medicinu, farmaciju i kozmetiku. Mehanizmi toksičnosti ovog
metala još uvek nisu dovoljno razjašnjeni. Do sada su najčešće ispitivani efekti pesticida koji sadrže
aluminijum, dok su rezultati koji opisuju dejstvo različitih koncentracija ovog metala na fitofagne
insekte retki, posebno u Srbiji. U našem eksperimentu, larve gubara (Limantria dispar L., Erebidae)
bile su izložene hroničnom dejstvu aluminijuma (tretman, T) u hrani (T1=50; T2 =250, T3=500 i T4=1000
microg/g suve hrane), od izleganja do trećeg dana petog larvenog stupnja (V3). Praćene su osobine
fitnesa (relativna brzina rasta - RGR, masa larve (V3) i vreme razvića - DT), kao prvi pokazatelji uticaja
aluminijuma na rast i razvoj.
Statistički značajna razlika je otkrivena kod svih osobina fitnesa na T2 i T4. Primećene su značajne razlike:
između kontrole - i tretmana (T1 i T2) za masu larve i DT; kontrole - i tretmana (T3 i T4) za RGR i DT; kao
i između T1 i T4 za RGR i masu larve. Na tretmanima T1, T2, T3 postoje značajne pozitivne korelacije
između mase larvi i RGR, dok na T2, T3 i T4 postoje značajne negativne korelacije između RGR i DT.
Promene osobina fitnesa insekata mogu biti prvi signal upozorenja na prisustvo aluminijuma u hrani",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "Chronic effect of dietary aluminum on fitness traits in Lymantria dispar L. (Erebidae), Hronični efekat aluminijuma u hrani na osobine fitnesa kod larvi Lymantria dispar L. (Erebidae)",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6524"
}

Vlahović, M., Matić, D., Ilijin, L., Mrdaković, M., Todorović, D., Mirčić, D.,& Perić Mataruga, V.. (2023). Chronic effect of dietary aluminum on fitness traits in Lymantria dispar L. (Erebidae). in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
Belgrade: Serbian Society of Toxicology., 89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6524

Vlahović M, Matić D, Ilijin L, Mrdaković M, Todorović D, Mirčić D, Perić Mataruga V. Chronic effect of dietary aluminum on fitness traits in Lymantria dispar L. (Erebidae). in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia. 2023;:89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6524 .

Vlahović, Milena, Matić, Dragana, Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Mirčić, Dejan, Perić Mataruga, Vesna, "Chronic effect of dietary aluminum on fitness traits in Lymantria dispar L. (Erebidae)" in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia (2023):89-90,
https://hdl.handle.net/21.15107/rcub_ibiss_6524 .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Mutić, Jelena
AU  - Đurđić, Slađana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6079
AB  - Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment
VL  - 273
DO  - 10.1016/j.cbpc.2023.109721
SP  - 109721
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Ilijin, Larisa and Mrdaković, Marija and Mutić, Jelena and Đurđić, Slađana and Perić Mataruga, Vesna",
year = "2023",
abstract = "Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment",
volume = "273",
doi = "10.1016/j.cbpc.2023.109721",
pages = "109721"
}

Matić, D., Vlahović, M., Grčić, A., Filipović, A., Ilijin, L., Mrdaković, M., Mutić, J., Đurđić, S.,& Perić Mataruga, V.. (2023). Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 273, 109721.
https://doi.org/10.1016/j.cbpc.2023.109721

Matić D, Vlahović M, Grčić A, Filipović A, Ilijin L, Mrdaković M, Mutić J, Đurđić S, Perić Mataruga V. Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2023;273:109721.
doi:10.1016/j.cbpc.2023.109721 .

Matić, Dragana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Ilijin, Larisa, Mrdaković, Marija, Mutić, Jelena, Đurđić, Slađana, Perić Mataruga, Vesna, "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 273 (2023):109721,
https://doi.org/10.1016/j.cbpc.2023.109721 . .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6074
AB  - Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.
PB  - San Diego: Academic Press Inc.
T2  - Environmental Research
T1  - Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature
IS  - 116461
IS  - Part 1
VL  - 236
DO  - 10.1016/j.envres.2023.116461
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2023",
abstract = "Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.",
publisher = "San Diego: Academic Press Inc.",
journal = "Environmental Research",
title = "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature",
number = "116461, Part 1",
volume = "236",
doi = "10.1016/j.envres.2023.116461"
}

Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2023). Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research
San Diego: Academic Press Inc.., 236(116461).
https://doi.org/10.1016/j.envres.2023.116461

Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Vlahović M, Perić Mataruga V. Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research. 2023;236(116461).
doi:10.1016/j.envres.2023.116461 .

Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature" in Environmental Research, 236, no. 116461 (2023),
https://doi.org/10.1016/j.envres.2023.116461 . .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5753
AB  - In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.
PB  - Academic Press
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species
VL  - 257
DO  - 10.1016/j.ecoenv.2023.114937
SP  - 114937
ER  - 

@article{
author = "Mrdaković, Marija and Filipović, Aleksandra and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Vlahović, Milena and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2023",
abstract = "In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.",
publisher = "Academic Press",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species",
volume = "257",
doi = "10.1016/j.ecoenv.2023.114937",
pages = "114937"
}

Mrdaković, M., Filipović, A., Ilijin, L., Grčić, A., Matić, D., Vlahović, M., Todorović, D.,& Perić Mataruga, V.. (2023). Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety
Academic Press., 257, 114937.
https://doi.org/10.1016/j.ecoenv.2023.114937

Mrdaković M, Filipović A, Ilijin L, Grčić A, Matić D, Vlahović M, Todorović D, Perić Mataruga V. Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety. 2023;257:114937.
doi:10.1016/j.ecoenv.2023.114937 .

Mrdaković, Marija, Filipović, Aleksandra, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Vlahović, Milena, Todorović, Dajana, Perić Mataruga, Vesna, "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species" in Ecotoxicology and Environmental Safety, 257 (2023):114937,
https://doi.org/10.1016/j.ecoenv.2023.114937 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5308
AB  - Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.
PB  - London: Nature Publishing Group
T2  - Scientific Reports
T1  - The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae
IS  - 12
DO  - 10.1038/s41598-022-26506-2
SP  - 21858
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Todorović, Dajana and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.",
publisher = "London: Nature Publishing Group",
journal = "Scientific Reports",
title = "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae",
number = "12",
doi = "10.1038/s41598-022-26506-2",
pages = "21858"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Todorović, D., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2022). The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports
London: Nature Publishing Group.(12), 21858.
https://doi.org/10.1038/s41598-022-26506-2

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Todorović D, Filipović A, Matić D, Perić Mataruga V. The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports. 2022;(12):21858.
doi:10.1038/s41598-022-26506-2 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Todorović, Dajana, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae" in Scientific Reports, no. 12 (2022):21858,
https://doi.org/10.1038/s41598-022-26506-2 . .

TY  - JOUR
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5361
AB  - Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.
PB  - Lahore: Zoological Society of Pakistan
T2  - Pakistan Journal of Zoology
T1  - The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae
DO  - 10.17582/journal.pjz/20211019131007
ER  - 

@article{
author = "Vlahović, Milena and Matić, Dragana and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Lazarević, Jelica and Perić Mataruga, Vesna",
year = "2022",
abstract = "Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.",
publisher = "Lahore: Zoological Society of Pakistan",
journal = "Pakistan Journal of Zoology",
title = "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae",
doi = "10.17582/journal.pjz/20211019131007"
}

Vlahović, M., Matić, D., Mrdaković, M., Ilijin, L., Grčić, A., Filipović, A., Lazarević, J.,& Perić Mataruga, V.. (2022). The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology
Lahore: Zoological Society of Pakistan..
https://doi.org/10.17582/journal.pjz/20211019131007

Vlahović M, Matić D, Mrdaković M, Ilijin L, Grčić A, Filipović A, Lazarević J, Perić Mataruga V. The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology. 2022;.
doi:10.17582/journal.pjz/20211019131007 .

Vlahović, Milena, Matić, Dragana, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Lazarević, Jelica, Perić Mataruga, Vesna, "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae" in Pakistan Journal of Zoology (2022),
https://doi.org/10.17582/journal.pjz/20211019131007 . .

TY  - CONF
AU  - Perić Mataruga, Vesna
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5314
AB  - Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field
SP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5314
ER  - 

@conference{
author = "Perić Mataruga, Vesna and Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra",
year = "2022",
abstract = "Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field",
pages = "3",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5314"
}

Perić Mataruga, V., Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Matić, D., Grčić, A.,& Filipović, A.. (2022). Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314

Perić Mataruga V, Todorović D, Ilijin L, Vlahović M, Mrdaković M, Matić D, Grčić A, Filipović A. Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

Perić Mataruga, Vesna, Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):3,
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

TY  - CONF
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Petković, Branka
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5313
AB  - Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field
SP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5313
ER  - 

@conference{
author = "Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Petković, Branka and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2022",
abstract = "Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field",
pages = "2",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5313"
}

Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Petković, B., Matić, D., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2022). Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313

Todorović D, Ilijin L, Vlahović M, Mrdaković M, Petković B, Matić D, Grčić A, Filipović A, Perić Mataruga V. Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Petković, Branka, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):2,
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

TY  - CONF
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5133
AB  - Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu.
AB  - Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
T1  - Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
SP  - 356
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5133
ER  - 

@conference{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu., Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae), Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)",
pages = "356",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5133"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D.,& Perić Mataruga, V.. (2022). Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Perić Mataruga V. Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Perić Mataruga, Vesna, "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):356,
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5132
AB  - Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација.
AB  - Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje
T1  - Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење
SP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5132
ER  - 

@conference{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација., Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje, Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење",
pages = "357",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5132"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2022). Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):357,
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4089
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S153204562100199X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4480
AB  - Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109172
SP  - 109172
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109172",
pages = "109172"
}

Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Todorović, D.,& Perić Mataruga, V.. (2021). Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 250, 109172.
https://doi.org/10.1016/j.cbpc.2021.109172

Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Todorović D, Perić Mataruga V. Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109172.
doi:10.1016/j.cbpc.2021.109172 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Todorović, Dajana, Perić Mataruga, Vesna, "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109172,
https://doi.org/10.1016/j.cbpc.2021.109172 . .

TY  - JOUR
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621001502
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4482
AB  - Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.
VL  - 249
DO  - 10.1016/j.cbpc.2021.109123
SP  - 109123
ER  - 

@article{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2021",
abstract = "Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.",
volume = "249",
doi = "10.1016/j.cbpc.2021.109123",
pages = "109123"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2021). Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 249, 109123.
https://doi.org/10.1016/j.cbpc.2021.109123

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Vlahović M, Perić Mataruga V. Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;249:109123.
doi:10.1016/j.cbpc.2021.109123 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 249 (2021):109123,
https://doi.org/10.1016/j.cbpc.2021.109123 . .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4913
AB  - INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene
SP  - 65
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4913
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene",
pages = "65-66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4913"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913

Mimica-Dukić N, Pajević S, Mandić A, Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Perić Mataruga V. Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):65-66,
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

TY  - CONF
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4912
AB  - INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations
SP  - 77
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4912
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Mrdaković, Marija and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations",
pages = "77-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4912"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Mrdaković, M.,& Perić Mataruga, V.. (2021). Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912

Mimica-Dukić N, Pajević S, Mandić A, Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Mrdaković M, Perić Mataruga V. Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Mrdaković, Marija, Perić Mataruga, Vesna, "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):77-78,
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

TY  - CONF
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4784
AB  - INTRODUCTION: Environmental temperature has a direct impact on the development of phytophagous insects, and an indirect, through influence on their host plant composition. Alkaline phosphatases (ALP) and total acid phosphatases (tot ACP) are midgut enzymes included in metabolic processes. Previous contact of population with various stressors, but also their ability to overcome the effects of the raised temperature (thermotolerance), can modify the response of these enzymes to increased environmental temperature.
OBJECTIVES: Our aim was to compare the differences in responses of midgut ALP and tot ACP and expression of their isoforms to increased environmental temperature with and without induced thermotolerance, in gypsy moth 5th instar caterpillars from unpolluted and polluted habitats.
METHOD / DESIGN: Caterpillars were hatched from egg masses collected in unpolluted (UP population) and polluted forest (PP population). They were reared at 23°C (PP23 and UP 23) and 28°C (PP28 and UP28) until 3rd day of 5th instar. In both populations a group of individuals were exposed to 28°C for 24 h (induced thermotolerance) at the beginning of the 4th instar. Afterwards they were returned to 23°C until the sacrification (PP23In and UP23In) or exposed to 28°C for 72h before sacrification on the 3rd day of 5th instar (PP28In and UP28In). The activity of enzymes was measured spectrophotometrically, using p-nitrophenyl phosphate (pNPP) as substrate, under alkaline conditions for ALP and acid conditions for tot ACP. Isoforms of both enzymes were detected on 12% polyacrylamide gel native PAGE. 
RESULTS: In UP groups, midgut ALP showed increased activity upon exposure to 28°C, with and without induced thermotolerance, while in PP caterpillars induced thermotolerance was the only factor that elevated ALP activity. Two way ANOVA analysis revealed that the interaction of temperature treatments and population origin (unpolluted vs polluted forest) was extremely significant (F3,67=27.6, p<0.0001) for changes in midgut ALP activity, as well as the individual influence of increased temperature (F3,67=30.9, p<0.0001) and the origin of the population (F1,67=28.6, p<0.0001). Three ALP isoforms were detected. Isoform 1 was present only in PP groups exposed to 28°C, second is present in all experimental groups, and the third showed lower band density in PP treatments in comparison to UP. In UP23In tot ACP activity was elevated, while in PP treatments it was decreased. The interaction of temperature and population origin was extremely significant for tot ACP activity (two way ANOVA, F3,72=10.48, p<0.0001), while their individual influence was not. Four isoforms of tot ACP were detected on gel. Isoform 1 was present only in PP groups, isoform 2 has higher density in both populations and all treatments in comparison to controls. High band density of isoform 3 is present in all experimental groups, while induced thermotolerance and increased temperature, in both population, increased band density of isoform 4.
CONCLUSIONS: Increased environmental temperature and induced thermotolerance have different effects on the activity of both enzymes in caterpillars from unpolluted and polluted habitats. ALP activity was more sensitive to thermal treatments in individuals originating from unpolluted forests, in comparison to those from polluted habitats, where, on the other hand, completely new isoform was detected upon exposure to increased temperature. Tot ACP activity was decreased in all treatments in caterpillars from polluted habitats and a new isoform band was detected on native gels, while in those from unpolluted forest, induced thermotolerance had the effect on the activity of tot ACP. Obtained results indicate the differences in sensitivity to increased environmental temperature between populations with different histories of exposure to pollution and that they must be considered as well.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021
T1  - Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4784
ER  - 

@conference{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION: Environmental temperature has a direct impact on the development of phytophagous insects, and an indirect, through influence on their host plant composition. Alkaline phosphatases (ALP) and total acid phosphatases (tot ACP) are midgut enzymes included in metabolic processes. Previous contact of population with various stressors, but also their ability to overcome the effects of the raised temperature (thermotolerance), can modify the response of these enzymes to increased environmental temperature.
OBJECTIVES: Our aim was to compare the differences in responses of midgut ALP and tot ACP and expression of their isoforms to increased environmental temperature with and without induced thermotolerance, in gypsy moth 5th instar caterpillars from unpolluted and polluted habitats.
METHOD / DESIGN: Caterpillars were hatched from egg masses collected in unpolluted (UP population) and polluted forest (PP population). They were reared at 23°C (PP23 and UP 23) and 28°C (PP28 and UP28) until 3rd day of 5th instar. In both populations a group of individuals were exposed to 28°C for 24 h (induced thermotolerance) at the beginning of the 4th instar. Afterwards they were returned to 23°C until the sacrification (PP23In and UP23In) or exposed to 28°C for 72h before sacrification on the 3rd day of 5th instar (PP28In and UP28In). The activity of enzymes was measured spectrophotometrically, using p-nitrophenyl phosphate (pNPP) as substrate, under alkaline conditions for ALP and acid conditions for tot ACP. Isoforms of both enzymes were detected on 12% polyacrylamide gel native PAGE. 
RESULTS: In UP groups, midgut ALP showed increased activity upon exposure to 28°C, with and without induced thermotolerance, while in PP caterpillars induced thermotolerance was the only factor that elevated ALP activity. Two way ANOVA analysis revealed that the interaction of temperature treatments and population origin (unpolluted vs polluted forest) was extremely significant (F3,67=27.6, p<0.0001) for changes in midgut ALP activity, as well as the individual influence of increased temperature (F3,67=30.9, p<0.0001) and the origin of the population (F1,67=28.6, p<0.0001). Three ALP isoforms were detected. Isoform 1 was present only in PP groups exposed to 28°C, second is present in all experimental groups, and the third showed lower band density in PP treatments in comparison to UP. In UP23In tot ACP activity was elevated, while in PP treatments it was decreased. The interaction of temperature and population origin was extremely significant for tot ACP activity (two way ANOVA, F3,72=10.48, p<0.0001), while their individual influence was not. Four isoforms of tot ACP were detected on gel. Isoform 1 was present only in PP groups, isoform 2 has higher density in both populations and all treatments in comparison to controls. High band density of isoform 3 is present in all experimental groups, while induced thermotolerance and increased temperature, in both population, increased band density of isoform 4.
CONCLUSIONS: Increased environmental temperature and induced thermotolerance have different effects on the activity of both enzymes in caterpillars from unpolluted and polluted habitats. ALP activity was more sensitive to thermal treatments in individuals originating from unpolluted forests, in comparison to those from polluted habitats, where, on the other hand, completely new isoform was detected upon exposure to increased temperature. Tot ACP activity was decreased in all treatments in caterpillars from polluted habitats and a new isoform band was detected on native gels, while in those from unpolluted forest, induced thermotolerance had the effect on the activity of tot ACP. Obtained results indicate the differences in sensitivity to increased environmental temperature between populations with different histories of exposure to pollution and that they must be considered as well.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021",
title = "Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4784"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Matić, D., Todorović, D.,& Perić Mataruga, V.. (2021). Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021
Novi Sad: Faculty of Sciences, University of Novi Sad., 64.
https://hdl.handle.net/21.15107/rcub_ibiss_4784

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Matić D, Todorović D, Perić Mataruga V. Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021. 2021;:64.
https://hdl.handle.net/21.15107/rcub_ibiss_4784 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Todorović, Dajana, Perić Mataruga, Vesna, "Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 (2021):64,
https://hdl.handle.net/21.15107/rcub_ibiss_4784 .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4283
AB  - Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.
PB  - Elsevier
T2  - Environmntal Pollution
T1  - Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants
VL  - 288
DO  - 10.1016/j.envpol.2021.117706
SP  - 117706
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.",
publisher = "Elsevier",
journal = "Environmntal Pollution",
title = "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants",
volume = "288",
doi = "10.1016/j.envpol.2021.117706",
pages = "117706"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution
Elsevier., 288, 117706.
https://doi.org/10.1016/j.envpol.2021.117706

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution. 2021;288:117706.
doi:10.1016/j.envpol.2021.117706 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants" in Environmntal Pollution, 288 (2021):117706,
https://doi.org/10.1016/j.envpol.2021.117706 . .

TY  - JOUR
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3935
UR  - https://link.springer.com/article/10.1134/S0022093020030072
AB  - A crucial enzyme in feeding and survival of Lymantria dispar (gypsy moth) caterpillars is β-glucosidase. Considering that this herbivorous species is one of the most damaging pests, a study of the enzyme that hydrolyzes plant polysaccharides is of invaluable significance. The knowledge of glucosidase alterations helps clarify our understanding of the insect pest feeding strategy in highly polluted environments. We sought to find out whether acute and chronic exposures to cadmium (10 and 30 μg Cd/g dry food), as well as the recovery from long-term treatment, influence the specific activity of midgut β-glucosidase. Cadmium ingestion did not alter enzyme activity compared to control, regardless of the treatment duration and metal concentration. The only statistically significant increase in enzyme activity was detected during the recovery after chronic treatment at 10 μg Cd/g dry food compared to the chronic cadmium effect at the same metal concentration. During chronic treatment at a lower cadmium concentration (10 μg Cd/g dry food), the phenotypic plasticity index was higher than at the recovery from the same concentration, while the variability of plasticity among these treatments was higher after recovery. Only one enzyme isoform was detected, which indicates its ability to hydrolyze several substrates. We concluded that this enzyme is probably essential for larval nutrition and survival in the presence of high amounts of cadmium in the food.
PB  - Springer Nature
T2  - Journal of Evolutionary Biochemistry and Physiology
T1  - Effect of cadmium dietary intake on midgut β-glucosidase of Lymantria dispar larvae
IS  - 3
VL  - 56
DO  - 10.1134/S0022093020030072
SP  - 243
EP  - 251
ER  - 

@article{
author = "Vlahović, Milena and Matić, Dragana and Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Lazarević, Jelica and Perić Mataruga, Vesna",
year = "2020",
abstract = "A crucial enzyme in feeding and survival of Lymantria dispar (gypsy moth) caterpillars is β-glucosidase. Considering that this herbivorous species is one of the most damaging pests, a study of the enzyme that hydrolyzes plant polysaccharides is of invaluable significance. The knowledge of glucosidase alterations helps clarify our understanding of the insect pest feeding strategy in highly polluted environments. We sought to find out whether acute and chronic exposures to cadmium (10 and 30 μg Cd/g dry food), as well as the recovery from long-term treatment, influence the specific activity of midgut β-glucosidase. Cadmium ingestion did not alter enzyme activity compared to control, regardless of the treatment duration and metal concentration. The only statistically significant increase in enzyme activity was detected during the recovery after chronic treatment at 10 μg Cd/g dry food compared to the chronic cadmium effect at the same metal concentration. During chronic treatment at a lower cadmium concentration (10 μg Cd/g dry food), the phenotypic plasticity index was higher than at the recovery from the same concentration, while the variability of plasticity among these treatments was higher after recovery. Only one enzyme isoform was detected, which indicates its ability to hydrolyze several substrates. We concluded that this enzyme is probably essential for larval nutrition and survival in the presence of high amounts of cadmium in the food.",
publisher = "Springer Nature",
journal = "Journal of Evolutionary Biochemistry and Physiology",
title = "Effect of cadmium dietary intake on midgut β-glucosidase of Lymantria dispar larvae",
number = "3",
volume = "56",
doi = "10.1134/S0022093020030072",
pages = "243-251"
}

Vlahović, M., Matić, D., Ilijin, L., Mrdaković, M., Todorović, D., Lazarević, J.,& Perić Mataruga, V.. (2020). Effect of cadmium dietary intake on midgut β-glucosidase of Lymantria dispar larvae. in Journal of Evolutionary Biochemistry and Physiology
Springer Nature., 56(3), 243-251.
https://doi.org/10.1134/S0022093020030072

Vlahović M, Matić D, Ilijin L, Mrdaković M, Todorović D, Lazarević J, Perić Mataruga V. Effect of cadmium dietary intake on midgut β-glucosidase of Lymantria dispar larvae. in Journal of Evolutionary Biochemistry and Physiology. 2020;56(3):243-251.
doi:10.1134/S0022093020030072 .

Vlahović, Milena, Matić, Dragana, Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Lazarević, Jelica, Perić Mataruga, Vesna, "Effect of cadmium dietary intake on midgut β-glucosidase of Lymantria dispar larvae" in Journal of Evolutionary Biochemistry and Physiology, 56, no. 3 (2020):243-251,
https://doi.org/10.1134/S0022093020030072 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3606
AB  - Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.
T2  - Ecological Indicators
T1  - Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment
VL  - 112
DO  - 10.1016/j.ecolind.2020.106136
SP  - 106136
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Mrdaković, Marija and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2020",
abstract = "Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.",
journal = "Ecological Indicators",
title = "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment",
volume = "112",
doi = "10.1016/j.ecolind.2020.106136",
pages = "106136"
}

Matić, D., Vlahović, M., Ilijin, L., Mrdaković, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2020). Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators, 112, 106136.
https://doi.org/10.1016/j.ecolind.2020.106136

Matić D, Vlahović M, Ilijin L, Mrdaković M, Grčić A, Filipović A, Perić Mataruga V. Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators. 2020;112:106136.
doi:10.1016/j.ecolind.2020.106136 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Mrdaković, Marija, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment" in Ecological Indicators, 112 (2020):106136,
https://doi.org/10.1016/j.ecolind.2020.106136 . .

TY  - JOUR
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Prokić, Marko
AU  - Matić, Dragana
AU  - Vlahović, Milena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0045653518322197?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3233
AB  - Cadmium (Cd) can display a variety of different effects on living organisms. The objectives of the present study were to investigate Cd bioaccumulation and differences in parameters of oxidative stress (activities of the enzymes: superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase, glutathione S-transferase, and amounts of non-enzymatic free sulfhydryl groups and total glutathione) and cholinesterase activity in larval brain and midgut tissues of the polyphagous forest insect Lymantria dispar collected from unpolluted and polluted oak forests. Fourth instar L. dispar caterpillars from the unpolluted forest had higher body mass but accumulated more Cd in comparison to caterpillars from the polluted forest. In both populations the midgut was more sensitive than the brain to the prooxidative effects of Cd. Enzyme activities and amounts of non-enzymatic parameters tended to be greater in midgut tissues than in the brain, except for cholinesterase activity. Parameters of oxidative stress had higher values in caterpillar tissues from the polluted than from the unpolluted oak forest. The observed differences between the two natural populations point to the importance of knowing the history of population exposure to environmental pollution when monitoring forest ecosystems.
PB  - Elsevier Ltd.
T2  - Chemosphere
T1  - Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests.
VL  - 218
DO  - 10.1016/j.chemosphere.2018.11.112
SP  - 416
EP  - 424
ER  - 

@article{
author = "Perić Mataruga, Vesna and Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Prokić, Marko and Matić, Dragana and Vlahović, Milena",
year = "2019",
abstract = "Cadmium (Cd) can display a variety of different effects on living organisms. The objectives of the present study were to investigate Cd bioaccumulation and differences in parameters of oxidative stress (activities of the enzymes: superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase, glutathione S-transferase, and amounts of non-enzymatic free sulfhydryl groups and total glutathione) and cholinesterase activity in larval brain and midgut tissues of the polyphagous forest insect Lymantria dispar collected from unpolluted and polluted oak forests. Fourth instar L. dispar caterpillars from the unpolluted forest had higher body mass but accumulated more Cd in comparison to caterpillars from the polluted forest. In both populations the midgut was more sensitive than the brain to the prooxidative effects of Cd. Enzyme activities and amounts of non-enzymatic parameters tended to be greater in midgut tissues than in the brain, except for cholinesterase activity. Parameters of oxidative stress had higher values in caterpillar tissues from the polluted than from the unpolluted oak forest. The observed differences between the two natural populations point to the importance of knowing the history of population exposure to environmental pollution when monitoring forest ecosystems.",
publisher = "Elsevier Ltd.",
journal = "Chemosphere",
title = "Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests.",
volume = "218",
doi = "10.1016/j.chemosphere.2018.11.112",
pages = "416-424"
}

Perić Mataruga, V., Ilijin, L., Mrdaković, M., Todorović, D., Prokić, M., Matić, D.,& Vlahović, M.. (2019). Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests.. in Chemosphere
Elsevier Ltd.., 218, 416-424.
https://doi.org/10.1016/j.chemosphere.2018.11.112

Perić Mataruga V, Ilijin L, Mrdaković M, Todorović D, Prokić M, Matić D, Vlahović M. Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests.. in Chemosphere. 2019;218:416-424.
doi:10.1016/j.chemosphere.2018.11.112 .

Perić Mataruga, Vesna, Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Prokić, Marko, Matić, Dragana, Vlahović, Milena, "Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests." in Chemosphere, 218 (2019):416-424,
https://doi.org/10.1016/j.chemosphere.2018.11.112 . .

TY  - THES
AU  - Matić, Dragana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3082
AB  - Razvoj industrije i poljoprivrede, kao i globalna urbanizacija, doveli su do pojave velikog broja polutanata u životnoj sredini, među kojima je i izuzetno toksični teški metal kadmijum. Cilj ove disertacije bila je procena larvi gubara (Lymantria dispar, L.) kao potencijalnog bioindikatora zagađenosti kadmijumom. Ispitani su odgovori izabranih fizioloških parametara na različitim nivoima biološke organizacije u prisustvu dve koncentracije metala (50 i 100 μg Cd/g suve hrane). Radi ispitivanja efekta porekla populacije, legla gubara sakupljena su na dve nezagađene lokacije (Kosmaj i Homoljske planine) i dve zagađene (pored Ibarske magistrale i borskog rudnika). Larve sa Kosmaja odlikuje veća osetljivost digestivnih enzima srednjeg creva (proteaza, alkalnih i kiselih fosfataza i nespecifičnih esteraza) na tretmane, naročito na nižu koncentraciju kadmijuma, u odnosu na one uz Ibarsku magistralu. Od ispitanih detoksifikacionih mehanizama u crevu, indukcija Hsp70 značajnija je kod kosmajskih larvi, a sinteza metalotioneina kod ibarskih. Trend sniženja aktivnosti antioksidativnih enzima, superoksid dismutaze i katalaze, uočen je kod obe populacije. U svim grupama tretiranim kadmijumom najveća količina ovog metala eliminiše se putem fecesa, ali intenzivnije u ibarskoj populaciji u odnosu na kosmajsku nakon tretmana sa 50 μg Cd/g suve hrane. Kod homoljskih gusenica tretmani kadmijumom doveli su do povećanja nivoa oštećenja DNK u hemocitama i dozno-zavisnog sniženja njihove vijabilnosti, dok je u populaciji iz Bora zabeleženo samo smanjenje vijabilnosti nakon primene više koncentracije metala. Štetni efekti kadmijuma i visoka cena detoksifikacionih mehanizama odrazili su se i na osobine fitnesa u vidu promena mase larvi, relativne brzine rasta i trajanja razvića kod svih populacija u različitom stepenu. Primena većine ispitanih parametara (specifične aktivnosti tripsina, nelizozomskih kiselih fosfataza i nespecifičnih esteraza, nivoa ekspresije Hsp70, vijabilnosti hemocita i nivoa oštećenja DNK) kao biomarkera zagađenosti životne sredine kadmijumom ograničena je na osetljive populacije gubara koje prethodno nisu bile izložene polutantima, dok bi koncentracija metalotioneina mogla biti podobna kao biomarker kod populacija sa lokacija kontaminiranih tokom dužeg perioda.
AB  - Industrial and agricultural development as well as global urbanization have caused considerable increase in the environmental pollution including extremely toxic heavy metal cadmium. The aim of this dissertation was to evaluate gypsy moth (Lymantria dispar, L.) larvae as bioindicators of cadmium contamination. Parameters at different levels of biological organization were assessed after chronic exposure of larvae to the metal (50 and 100 μg Cd/g dry food). To investigate the effects of population origin, gypsy moth egg-masses were collected from two unpolluted localities on Kosmaj and Homolje mountains and two polluted sites near the busy Ibar highway and Bor copper mine. Midgut digestive enzymes (proteases, alkaline and acid phosphatases and nonspecific esterases) of Kosmaj larvae were more sensitive to cadmium, especially to lower metal concentration, compared to those of Ibar larvae. Hsp70 induction was the most prominent midgut detoxification mechanism against cadmium in the population from the unpolluted forest, whereas metallothionein synthesis was the most significant in larvae from the polluted site. Activities of antioxidative enzymes, superoxide dismutase and catalase, exhibited a decreasing trend in both populations. The primary route of cadmium elimination was via feces in all metal-fed groups, but after an exposure to 50 μg Cd/g dry food metal content was significantly higher in feces of Kosmaj larvae compared to those from the highway locality. Exposure of caterpillars from the Homolje mountains to the metal led to the higher level of DNA damage in the hemocytes and a dose-dependent decrease in cell viability, whereas in Bor population only hemocyte viability was reduced after the treatment with 100 μg Cd/g dry food. Devastating cadmium effects and energetically expensive detoxification mechanisms affected the fitness traits (larval mass, relative growth rate or development parameters) in all populations. The application of selected parameters (specific activities of trypsin, nonlisosomal acid phosphatases and nonspecific esterases, Hsp70 expression, hemocyte viability and DNA damage level) as biomarkers of environmental cadmium pollution is limited mostly to sensitive populations that have not been previously exposed to pollutants. Metallothionein concentration might be suitable as a biomarker for the populations at locations that have been contaminated for a long period.
PB  - Belgrade: Universiy of Belgrade, Faculty of Biology
T2  - Universiy of Belgrade, Faculty of Biology
T1  - Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK
T1  - Potential role of gypsy moth (Lymantria dispar, L.) as a bioindicator of environmental pollution: the impact of cadmium chronic exposure on the development, midgut digestive enzyme activities, detoxification mechanisms and DNA damage level
SP  - 1
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3082
ER  - 

@phdthesis{
author = "Matić, Dragana",
year = "2018",
abstract = "Razvoj industrije i poljoprivrede, kao i globalna urbanizacija, doveli su do pojave velikog broja polutanata u životnoj sredini, među kojima je i izuzetno toksični teški metal kadmijum. Cilj ove disertacije bila je procena larvi gubara (Lymantria dispar, L.) kao potencijalnog bioindikatora zagađenosti kadmijumom. Ispitani su odgovori izabranih fizioloških parametara na različitim nivoima biološke organizacije u prisustvu dve koncentracije metala (50 i 100 μg Cd/g suve hrane). Radi ispitivanja efekta porekla populacije, legla gubara sakupljena su na dve nezagađene lokacije (Kosmaj i Homoljske planine) i dve zagađene (pored Ibarske magistrale i borskog rudnika). Larve sa Kosmaja odlikuje veća osetljivost digestivnih enzima srednjeg creva (proteaza, alkalnih i kiselih fosfataza i nespecifičnih esteraza) na tretmane, naročito na nižu koncentraciju kadmijuma, u odnosu na one uz Ibarsku magistralu. Od ispitanih detoksifikacionih mehanizama u crevu, indukcija Hsp70 značajnija je kod kosmajskih larvi, a sinteza metalotioneina kod ibarskih. Trend sniženja aktivnosti antioksidativnih enzima, superoksid dismutaze i katalaze, uočen je kod obe populacije. U svim grupama tretiranim kadmijumom najveća količina ovog metala eliminiše se putem fecesa, ali intenzivnije u ibarskoj populaciji u odnosu na kosmajsku nakon tretmana sa 50 μg Cd/g suve hrane. Kod homoljskih gusenica tretmani kadmijumom doveli su do povećanja nivoa oštećenja DNK u hemocitama i dozno-zavisnog sniženja njihove vijabilnosti, dok je u populaciji iz Bora zabeleženo samo smanjenje vijabilnosti nakon primene više koncentracije metala. Štetni efekti kadmijuma i visoka cena detoksifikacionih mehanizama odrazili su se i na osobine fitnesa u vidu promena mase larvi, relativne brzine rasta i trajanja razvića kod svih populacija u različitom stepenu. Primena većine ispitanih parametara (specifične aktivnosti tripsina, nelizozomskih kiselih fosfataza i nespecifičnih esteraza, nivoa ekspresije Hsp70, vijabilnosti hemocita i nivoa oštećenja DNK) kao biomarkera zagađenosti životne sredine kadmijumom ograničena je na osetljive populacije gubara koje prethodno nisu bile izložene polutantima, dok bi koncentracija metalotioneina mogla biti podobna kao biomarker kod populacija sa lokacija kontaminiranih tokom dužeg perioda., Industrial and agricultural development as well as global urbanization have caused considerable increase in the environmental pollution including extremely toxic heavy metal cadmium. The aim of this dissertation was to evaluate gypsy moth (Lymantria dispar, L.) larvae as bioindicators of cadmium contamination. Parameters at different levels of biological organization were assessed after chronic exposure of larvae to the metal (50 and 100 μg Cd/g dry food). To investigate the effects of population origin, gypsy moth egg-masses were collected from two unpolluted localities on Kosmaj and Homolje mountains and two polluted sites near the busy Ibar highway and Bor copper mine. Midgut digestive enzymes (proteases, alkaline and acid phosphatases and nonspecific esterases) of Kosmaj larvae were more sensitive to cadmium, especially to lower metal concentration, compared to those of Ibar larvae. Hsp70 induction was the most prominent midgut detoxification mechanism against cadmium in the population from the unpolluted forest, whereas metallothionein synthesis was the most significant in larvae from the polluted site. Activities of antioxidative enzymes, superoxide dismutase and catalase, exhibited a decreasing trend in both populations. The primary route of cadmium elimination was via feces in all metal-fed groups, but after an exposure to 50 μg Cd/g dry food metal content was significantly higher in feces of Kosmaj larvae compared to those from the highway locality. Exposure of caterpillars from the Homolje mountains to the metal led to the higher level of DNA damage in the hemocytes and a dose-dependent decrease in cell viability, whereas in Bor population only hemocyte viability was reduced after the treatment with 100 μg Cd/g dry food. Devastating cadmium effects and energetically expensive detoxification mechanisms affected the fitness traits (larval mass, relative growth rate or development parameters) in all populations. The application of selected parameters (specific activities of trypsin, nonlisosomal acid phosphatases and nonspecific esterases, Hsp70 expression, hemocyte viability and DNA damage level) as biomarkers of environmental cadmium pollution is limited mostly to sensitive populations that have not been previously exposed to pollutants. Metallothionein concentration might be suitable as a biomarker for the populations at locations that have been contaminated for a long period.",
publisher = "Belgrade: Universiy of Belgrade, Faculty of Biology",
journal = "Universiy of Belgrade, Faculty of Biology",
title = "Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK, Potential role of gypsy moth (Lymantria dispar, L.) as a bioindicator of environmental pollution: the impact of cadmium chronic exposure on the development, midgut digestive enzyme activities, detoxification mechanisms and DNA damage level",
pages = "1-135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3082"
}

Matić, D.. (2018). Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK. in Universiy of Belgrade, Faculty of Biology
Belgrade: Universiy of Belgrade, Faculty of Biology., 1-135.
https://hdl.handle.net/21.15107/rcub_ibiss_3082

Matić D. Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK. in Universiy of Belgrade, Faculty of Biology. 2018;:1-135.
https://hdl.handle.net/21.15107/rcub_ibiss_3082 .

Matić, Dragana, "Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK" in Universiy of Belgrade, Faculty of Biology (2018):1-135,
https://hdl.handle.net/21.15107/rcub_ibiss_3082 .

TY  - CONF
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3962
AB  - Razvoj industrije je tokom poslednjih decenija doveo do povećanja koncentracije izuzetno toksičnog teškog metala kadmijuma u životnoj sredini, što je uslovilo potrebu za stalnim unapređivanjem biomonitoringa. Kod insekata se oralno uneti kadmijum akumulira primarno u crevu, gde utiče na aktivnost digestivnih enzima. Cilj ove studije bio je proceniti specifične aktivnosti tripsina i leucin aminopeptidaze (LAP) i detektovati njihove izoforme u srednjem crevu larvi Lymantria dispar kao potencijalne biomarkere zagađenosti kadmijumom u zavisnosti od porekla populacije. Legla gubara sakupljena su na dve lokacije – u nezagađenoj šumi na planini Kosmaj, 60 km od Beograda, i na zagađenoj lokaciji uz Ibarsku magistralu u Beogradu. Jedinke su hronično bile izložene koncentracijama od 50 i 100 μg Cd/g suve hrane od izleganja do četvrtog stupnja, kada su žrtvovane. U populaciji sa Kosmaja oba tretmana su dovela do sniženja aktivnosti tripsina i LAP, pri čemu je aktivnost LAP na višoj koncentraciji metala bila značajno viša u odnosu na nižu koncentraciju. Kod larvi sa zagađenog lokaliteta zabeležen je samo rast aktivnosti LAP nakon tretmana sa 50 μg Cd/g suve hrane. Tri izoforme tripsina i šest izoformi LAP detektovano je u obe populacije u različitom broju i intenzitetu zavisno od koncentracije kadmijuma. Rezultati sugerišu da bi parametri aktivnosti tripsina mogli biti adekvatni biomarkeri kontaminacije kadmijumom u populacijama koje prethodno nisu bile izložene zagađujućim supstancama.
PB  - Belgrade: Serbian Biological Society
C3  - Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156
T1  - Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu
SP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3962
ER  - 

@conference{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Mrdaković, Marija and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2018",
abstract = "Razvoj industrije je tokom poslednjih decenija doveo do povećanja koncentracije izuzetno toksičnog teškog metala kadmijuma u životnoj sredini, što je uslovilo potrebu za stalnim unapređivanjem biomonitoringa. Kod insekata se oralno uneti kadmijum akumulira primarno u crevu, gde utiče na aktivnost digestivnih enzima. Cilj ove studije bio je proceniti specifične aktivnosti tripsina i leucin aminopeptidaze (LAP) i detektovati njihove izoforme u srednjem crevu larvi Lymantria dispar kao potencijalne biomarkere zagađenosti kadmijumom u zavisnosti od porekla populacije. Legla gubara sakupljena su na dve lokacije – u nezagađenoj šumi na planini Kosmaj, 60 km od Beograda, i na zagađenoj lokaciji uz Ibarsku magistralu u Beogradu. Jedinke su hronično bile izložene koncentracijama od 50 i 100 μg Cd/g suve hrane od izleganja do četvrtog stupnja, kada su žrtvovane. U populaciji sa Kosmaja oba tretmana su dovela do sniženja aktivnosti tripsina i LAP, pri čemu je aktivnost LAP na višoj koncentraciji metala bila značajno viša u odnosu na nižu koncentraciju. Kod larvi sa zagađenog lokaliteta zabeležen je samo rast aktivnosti LAP nakon tretmana sa 50 μg Cd/g suve hrane. Tri izoforme tripsina i šest izoformi LAP detektovano je u obe populacije u različitom broju i intenzitetu zavisno od koncentracije kadmijuma. Rezultati sugerišu da bi parametri aktivnosti tripsina mogli biti adekvatni biomarkeri kontaminacije kadmijumom u populacijama koje prethodno nisu bile izložene zagađujućim supstancama.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156",
title = "Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3962"
}

Matić, D., Vlahović, M., Ilijin, L., Mrdaković, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2018). Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu. in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156
Belgrade: Serbian Biological Society., 156.
https://hdl.handle.net/21.15107/rcub_ibiss_3962

Matić D, Vlahović M, Ilijin L, Mrdaković M, Grčić A, Filipović A, Perić Mataruga V. Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu. in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156. 2018;:156.
https://hdl.handle.net/21.15107/rcub_ibiss_3962 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Mrdaković, Marija, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu" in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156 (2018):156,
https://hdl.handle.net/21.15107/rcub_ibiss_3962 .

TY  - CONF
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Grčić, Anja
AU  - Perić Mataruga, Vesna
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3955
AB  - Cadmium ranks among the most toxic metals accumulating in the environment. We are searching for sensitive biomarkers relevant to forest ecosystems using gypsy moth, as it is a highly adapted and very prevalent insect species. We determined the activity and isozyme pattern of non-specific esterases in the midgut on the 3rd day of fourth instar larvae after exposure to cadmium (50 and 100 μg Cd/g dry food, Cd1 and Cd2, respectively) from hatching until sacrifice. The larvae originated from two locations, an unpolluted forest on Kosmaj mountain, 50 km far from Belgrade (UP), and the polluted Lipovica forest,
located close to a main highway near Belgrade (PP). Published data about the role of esterases in metal detoxification are insufficient. The zymogram revealed at least seven isoforms. In larvae from the unpolluted locality both levels of cadmium (50 and 100 μg Cd/g dry food) led to significant inhibition of esterase activity compared to controls, while no effect of cadmium treatment occurred in caterpillars from the polluted locality. Enzyme activity in non-treated larvae was significantly higher in the Kosmaj population. The presence and intensity of the isoforms varied depending on cadmium treatment. The alterations and abundance depended on the metal concentration and pointed to a multifunctional role and possible involvement in the wide spectrum of physiological processes in stressful conditions. Therefore, specific activity of midgut esterase can be considered as a potential biomarker of dietary cadmium only in populations that originate from unpolluted sites.
PB  - Brussels, Belgium: Longdom Publishing
C3  - 15th Euro Global Summit on Toxicology and Applied Pharmacology; July 02-04, 2018 in Berlin, Germany; Journal of Clinical Toxicology
T1  - Influence of chronic dietary exposure to cadmium on midgut esterases in gypsy moth (Lymantria dispar L.) caterpillars
VL  - 8
DO  - 10.4172/2161-0495-C1-028
SP  - 103
ER  - 

@conference{
author = "Vlahović, Milena and Matić, Dragana and Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Grčić, Anja and Perić Mataruga, Vesna",
year = "2018",
abstract = "Cadmium ranks among the most toxic metals accumulating in the environment. We are searching for sensitive biomarkers relevant to forest ecosystems using gypsy moth, as it is a highly adapted and very prevalent insect species. We determined the activity and isozyme pattern of non-specific esterases in the midgut on the 3rd day of fourth instar larvae after exposure to cadmium (50 and 100 μg Cd/g dry food, Cd1 and Cd2, respectively) from hatching until sacrifice. The larvae originated from two locations, an unpolluted forest on Kosmaj mountain, 50 km far from Belgrade (UP), and the polluted Lipovica forest,
located close to a main highway near Belgrade (PP). Published data about the role of esterases in metal detoxification are insufficient. The zymogram revealed at least seven isoforms. In larvae from the unpolluted locality both levels of cadmium (50 and 100 μg Cd/g dry food) led to significant inhibition of esterase activity compared to controls, while no effect of cadmium treatment occurred in caterpillars from the polluted locality. Enzyme activity in non-treated larvae was significantly higher in the Kosmaj population. The presence and intensity of the isoforms varied depending on cadmium treatment. The alterations and abundance depended on the metal concentration and pointed to a multifunctional role and possible involvement in the wide spectrum of physiological processes in stressful conditions. Therefore, specific activity of midgut esterase can be considered as a potential biomarker of dietary cadmium only in populations that originate from unpolluted sites.",
publisher = "Brussels, Belgium: Longdom Publishing",
journal = "15th Euro Global Summit on Toxicology and Applied Pharmacology; July 02-04, 2018 in Berlin, Germany; Journal of Clinical Toxicology",
title = "Influence of chronic dietary exposure to cadmium on midgut esterases in gypsy moth (Lymantria dispar L.) caterpillars",
volume = "8",
doi = "10.4172/2161-0495-C1-028",
pages = "103"
}

Vlahović, M., Matić, D., Ilijin, L., Mrdaković, M., Todorović, D., Grčić, A.,& Perić Mataruga, V.. (2018). Influence of chronic dietary exposure to cadmium on midgut esterases in gypsy moth (Lymantria dispar L.) caterpillars. in 15th Euro Global Summit on Toxicology and Applied Pharmacology; July 02-04, 2018 in Berlin, Germany; Journal of Clinical Toxicology
Brussels, Belgium: Longdom Publishing., 8, 103.
https://doi.org/10.4172/2161-0495-C1-028

Vlahović M, Matić D, Ilijin L, Mrdaković M, Todorović D, Grčić A, Perić Mataruga V. Influence of chronic dietary exposure to cadmium on midgut esterases in gypsy moth (Lymantria dispar L.) caterpillars. in 15th Euro Global Summit on Toxicology and Applied Pharmacology; July 02-04, 2018 in Berlin, Germany; Journal of Clinical Toxicology. 2018;8:103.
doi:10.4172/2161-0495-C1-028 .

Vlahović, Milena, Matić, Dragana, Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Grčić, Anja, Perić Mataruga, Vesna, "Influence of chronic dietary exposure to cadmium on midgut esterases in gypsy moth (Lymantria dispar L.) caterpillars" in 15th Euro Global Summit on Toxicology and Applied Pharmacology; July 02-04, 2018 in Berlin, Germany; Journal of Clinical Toxicology, 8 (2018):103,
https://doi.org/10.4172/2161-0495-C1-028 . .