TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Mutić, Jelena
AU  - Đurđić, Slađana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6079
AB  - Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment
VL  - 273
DO  - 10.1016/j.cbpc.2023.109721
SP  - 109721
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Ilijin, Larisa and Mrdaković, Marija and Mutić, Jelena and Đurđić, Slađana and Perić Mataruga, Vesna",
year = "2023",
abstract = "Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment",
volume = "273",
doi = "10.1016/j.cbpc.2023.109721",
pages = "109721"
}

Matić, D., Vlahović, M., Grčić, A., Filipović, A., Ilijin, L., Mrdaković, M., Mutić, J., Đurđić, S.,& Perić Mataruga, V.. (2023). Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 273, 109721.
https://doi.org/10.1016/j.cbpc.2023.109721

Matić D, Vlahović M, Grčić A, Filipović A, Ilijin L, Mrdaković M, Mutić J, Đurđić S, Perić Mataruga V. Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2023;273:109721.
doi:10.1016/j.cbpc.2023.109721 .

Matić, Dragana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Ilijin, Larisa, Mrdaković, Marija, Mutić, Jelena, Đurđić, Slađana, Perić Mataruga, Vesna, "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 273 (2023):109721,
https://doi.org/10.1016/j.cbpc.2023.109721 . .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6074
AB  - Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.
PB  - San Diego: Academic Press Inc.
T2  - Environmental Research
T1  - Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature
IS  - 116461
IS  - Part 1
VL  - 236
DO  - 10.1016/j.envres.2023.116461
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2023",
abstract = "Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.",
publisher = "San Diego: Academic Press Inc.",
journal = "Environmental Research",
title = "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature",
number = "116461, Part 1",
volume = "236",
doi = "10.1016/j.envres.2023.116461"
}

Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2023). Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research
San Diego: Academic Press Inc.., 236(116461).
https://doi.org/10.1016/j.envres.2023.116461

Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Vlahović M, Perić Mataruga V. Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research. 2023;236(116461).
doi:10.1016/j.envres.2023.116461 .

Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature" in Environmental Research, 236, no. 116461 (2023),
https://doi.org/10.1016/j.envres.2023.116461 . .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5753
AB  - In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.
PB  - Academic Press
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species
VL  - 257
DO  - 10.1016/j.ecoenv.2023.114937
SP  - 114937
ER  - 

@article{
author = "Mrdaković, Marija and Filipović, Aleksandra and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Vlahović, Milena and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2023",
abstract = "In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.",
publisher = "Academic Press",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species",
volume = "257",
doi = "10.1016/j.ecoenv.2023.114937",
pages = "114937"
}

Mrdaković, M., Filipović, A., Ilijin, L., Grčić, A., Matić, D., Vlahović, M., Todorović, D.,& Perić Mataruga, V.. (2023). Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety
Academic Press., 257, 114937.
https://doi.org/10.1016/j.ecoenv.2023.114937

Mrdaković M, Filipović A, Ilijin L, Grčić A, Matić D, Vlahović M, Todorović D, Perić Mataruga V. Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety. 2023;257:114937.
doi:10.1016/j.ecoenv.2023.114937 .

Mrdaković, Marija, Filipović, Aleksandra, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Vlahović, Milena, Todorović, Dajana, Perić Mataruga, Vesna, "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species" in Ecotoxicology and Environmental Safety, 257 (2023):114937,
https://doi.org/10.1016/j.ecoenv.2023.114937 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5308
AB  - Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.
PB  - London: Nature Publishing Group
T2  - Scientific Reports
T1  - The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae
IS  - 12
DO  - 10.1038/s41598-022-26506-2
SP  - 21858
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Todorović, Dajana and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.",
publisher = "London: Nature Publishing Group",
journal = "Scientific Reports",
title = "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae",
number = "12",
doi = "10.1038/s41598-022-26506-2",
pages = "21858"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Todorović, D., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2022). The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports
London: Nature Publishing Group.(12), 21858.
https://doi.org/10.1038/s41598-022-26506-2

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Todorović D, Filipović A, Matić D, Perić Mataruga V. The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports. 2022;(12):21858.
doi:10.1038/s41598-022-26506-2 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Todorović, Dajana, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae" in Scientific Reports, no. 12 (2022):21858,
https://doi.org/10.1038/s41598-022-26506-2 . .

TY  - JOUR
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5361
AB  - Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.
PB  - Lahore: Zoological Society of Pakistan
T2  - Pakistan Journal of Zoology
T1  - The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae
DO  - 10.17582/journal.pjz/20211019131007
ER  - 

@article{
author = "Vlahović, Milena and Matić, Dragana and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Lazarević, Jelica and Perić Mataruga, Vesna",
year = "2022",
abstract = "Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.",
publisher = "Lahore: Zoological Society of Pakistan",
journal = "Pakistan Journal of Zoology",
title = "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae",
doi = "10.17582/journal.pjz/20211019131007"
}

Vlahović, M., Matić, D., Mrdaković, M., Ilijin, L., Grčić, A., Filipović, A., Lazarević, J.,& Perić Mataruga, V.. (2022). The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology
Lahore: Zoological Society of Pakistan..
https://doi.org/10.17582/journal.pjz/20211019131007

Vlahović M, Matić D, Mrdaković M, Ilijin L, Grčić A, Filipović A, Lazarević J, Perić Mataruga V. The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology. 2022;.
doi:10.17582/journal.pjz/20211019131007 .

Vlahović, Milena, Matić, Dragana, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Lazarević, Jelica, Perić Mataruga, Vesna, "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae" in Pakistan Journal of Zoology (2022),
https://doi.org/10.17582/journal.pjz/20211019131007 . .

TY  - CONF
AU  - Perić Mataruga, Vesna
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5314
AB  - Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field
SP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5314
ER  - 

@conference{
author = "Perić Mataruga, Vesna and Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra",
year = "2022",
abstract = "Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field",
pages = "3",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5314"
}

Perić Mataruga, V., Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Matić, D., Grčić, A.,& Filipović, A.. (2022). Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314

Perić Mataruga V, Todorović D, Ilijin L, Vlahović M, Mrdaković M, Matić D, Grčić A, Filipović A. Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

Perić Mataruga, Vesna, Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):3,
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

TY  - CONF
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Petković, Branka
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5313
AB  - Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field
SP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5313
ER  - 

@conference{
author = "Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Petković, Branka and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2022",
abstract = "Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field",
pages = "2",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5313"
}

Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Petković, B., Matić, D., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2022). Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313

Todorović D, Ilijin L, Vlahović M, Mrdaković M, Petković B, Matić D, Grčić A, Filipović A, Perić Mataruga V. Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Petković, Branka, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):2,
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

TY  - CONF
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5133
AB  - Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu.
AB  - Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
T1  - Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
SP  - 356
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5133
ER  - 

@conference{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu., Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae), Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)",
pages = "356",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5133"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D.,& Perić Mataruga, V.. (2022). Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Perić Mataruga V. Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Perić Mataruga, Vesna, "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):356,
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5132
AB  - Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација.
AB  - Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje
T1  - Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење
SP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5132
ER  - 

@conference{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација., Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje, Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење",
pages = "357",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5132"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2022). Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):357,
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4089
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=en
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4409
AB  - In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch - off in the expression of constitutive and/or inducible HSP70 isoforms.
T2  - Anais da Academia Brasileira de Ciencias
T1  - Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.
IS  - 2
VL  - 93
DO  - 10.1590/0001-3765202120190118
SP  - e20190118
ER  - 

@article{
author = "Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch - off in the expression of constitutive and/or inducible HSP70 isoforms.",
journal = "Anais da Academia Brasileira de Ciencias",
title = "Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.",
number = "2",
volume = "93",
doi = "10.1590/0001-3765202120190118",
pages = "e20190118"
}

Ilijin, L., Mrdaković, M., Todorović, D., Vlahović, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2021). Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.. in Anais da Academia Brasileira de Ciencias, 93(2), e20190118.
https://doi.org/10.1590/0001-3765202120190118

Ilijin L, Mrdaković M, Todorović D, Vlahović M, Grčić A, Filipović A, Perić Mataruga V. Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.. in Anais da Academia Brasileira de Ciencias. 2021;93(2):e20190118.
doi:10.1590/0001-3765202120190118 .

Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields." in Anais da Academia Brasileira de Ciencias, 93, no. 2 (2021):e20190118,
https://doi.org/10.1590/0001-3765202120190118 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S153204562100199X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4480
AB  - Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109172
SP  - 109172
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109172",
pages = "109172"
}

Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Todorović, D.,& Perić Mataruga, V.. (2021). Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 250, 109172.
https://doi.org/10.1016/j.cbpc.2021.109172

Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Todorović D, Perić Mataruga V. Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109172.
doi:10.1016/j.cbpc.2021.109172 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Todorović, Dajana, Perić Mataruga, Vesna, "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109172,
https://doi.org/10.1016/j.cbpc.2021.109172 . .

TY  - JOUR
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621001502
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4482
AB  - Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.
VL  - 249
DO  - 10.1016/j.cbpc.2021.109123
SP  - 109123
ER  - 

@article{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2021",
abstract = "Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.",
volume = "249",
doi = "10.1016/j.cbpc.2021.109123",
pages = "109123"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2021). Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 249, 109123.
https://doi.org/10.1016/j.cbpc.2021.109123

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Vlahović M, Perić Mataruga V. Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;249:109123.
doi:10.1016/j.cbpc.2021.109123 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 249 (2021):109123,
https://doi.org/10.1016/j.cbpc.2021.109123 . .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4913
AB  - INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene
SP  - 65
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4913
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene",
pages = "65-66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4913"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913

Mimica-Dukić N, Pajević S, Mandić A, Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Perić Mataruga V. Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):65-66,
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

TY  - CONF
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4912
AB  - INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations
SP  - 77
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4912
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Mrdaković, Marija and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations",
pages = "77-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4912"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Mrdaković, M.,& Perić Mataruga, V.. (2021). Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912

Mimica-Dukić N, Pajević S, Mandić A, Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Mrdaković M, Perić Mataruga V. Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Mrdaković, Marija, Perić Mataruga, Vesna, "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):77-78,
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

TY  - THES
AU  - Filipović, Aleksandra
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4668
AB  - Fluoranten je jedan od najrasprostranjenijih policikličnih aromatičnih ugljovodonika, prisutan u lišću različitih biljaka kojima se hrane larve polifagnih vrsta insekata Lymantria dispar i Euproctis chrysorrhoea. Cilj ove doktorske disertacije je sticanje uvida u obrasce fizioloških odgovora larvi L. dispar i E. chrysorrhoea (Lepidoptera: Erebidae) na prisustvo sredinski relevantnih koncentracija fluorantena u hrani i rasvetljavanje mehanizama odbrane od njegovog štetnog delovanja, što doprinosi sagledavanju načina njihovog prilagođavanja stresnim uslovima u okruženju. Ispitivan je hronični uticaj dve koncentracije fluorantena (6,7 i 67 ng/g suve mase hrane) na larve L. dispar i E. chrysorrhoea, poreklom iz prirodnih populacija. Antioksidativni i detoksifikacioni enzimi srednjeg creva larvi su pokazali promene aktivnosti pod uticajem fluorantena, sa izraženijim odgovorima u tkivu srednjeg creva, kao i razlike u obrascima odgovora između vrsta. Uočeno je smanjenje ekspresije Hsp70 u celom srednjem crevu, a različiti odgovori Hsp70 u mozgu larvi. Kod obe vrste je došlo i do značajnih promena aktivnosti digestivnih enzima i njihovih izoformi u srednjem crevu larvi izlaganih fluorantenu. Detektovana je inhibicija aktivnosti određenih digestivnih enzima i njihovih izoformi kod larvi L. dispar, dok je kod larvi E. chrysorrhoea zabeleženo povećanje aktivnosti većeg broja enzima ove grupe, kao i ekspresija izoformi pojedinih enzima kod larvi izlaganih fluorantenu. Kanonijskom diskriminacionom analizom su potvrđene razlike između vrsta kada su u pitanju odgovori antioksidativnih i digestivnih enzima na prisustvo fluorantena u ishrani. Alokacija energetskih resursa ka mehanizmima odbrane, u prisustvu fluorantena, odrazila se na osobine životne istorije larvi obe vrste.
AB  - Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbons, present in the leaves of various plants used for food by larvae of polyphagous insect species Lymantria dispar and Euproctis chrysorrhoea. The aim of dissertation was to gain insight into the patterns of physiological responses of L. dispar and E. chrysorrhoea larvae (Lepidoptera: Erebidae) to the presence of environmentally relevant concentrations of fluoranthene in food and to elucidate the mechanisms of defense against its harmful effects, which contributes to perceive the way they adjust to stressful environmental conditions. Chronic effects of fluoranthene (6.7 and 67 ng/g dry food weight) on L. dispar and E. chrysorrhoea larvae, originating from natural populations, were examined. Antioxidative and detoxification enzymes of midgut showed changes of activity under the influence of fluoranthene, with more pronounced responses in midgut tissue, and between-species differences in patterns of responses. Decreased expression of Hsp70 in the midgut, and different responses of Hsp70 in the brain of larvae were noticed. Significant changes of the activity of digestive enzymes and their isoforms in the midgut of larvae of both species exposed to fluoranthene were noticed. Exposure to fluorantene inhibited the activity of certain digestive enzymes and their isoforms of L. dispar larvae, and increased the activity of a number of enzymes and expression of isoforms of certain enzymes of E. chrysorrhoea larvae. Canonical discriminant analysis confirmed between-species differences in responses of antioxidative and digestive enzymes to the presence of fluoranthene in the diet. The allocation of energy resources to defense mechanisms affected the life history traits of treated larvae of both species.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena
T1  - Physiological adjustment of larvae of gypsy moth, Lymantria dispar L., and brown-tail moth, Euproctis chrysorrhoea L., (Lepidoptera) to the effects of fluoranthene
SP  - 1
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4668
ER  - 

@phdthesis{
author = "Filipović, Aleksandra",
year = "2021",
abstract = "Fluoranten je jedan od najrasprostranjenijih policikličnih aromatičnih ugljovodonika, prisutan u lišću različitih biljaka kojima se hrane larve polifagnih vrsta insekata Lymantria dispar i Euproctis chrysorrhoea. Cilj ove doktorske disertacije je sticanje uvida u obrasce fizioloških odgovora larvi L. dispar i E. chrysorrhoea (Lepidoptera: Erebidae) na prisustvo sredinski relevantnih koncentracija fluorantena u hrani i rasvetljavanje mehanizama odbrane od njegovog štetnog delovanja, što doprinosi sagledavanju načina njihovog prilagođavanja stresnim uslovima u okruženju. Ispitivan je hronični uticaj dve koncentracije fluorantena (6,7 i 67 ng/g suve mase hrane) na larve L. dispar i E. chrysorrhoea, poreklom iz prirodnih populacija. Antioksidativni i detoksifikacioni enzimi srednjeg creva larvi su pokazali promene aktivnosti pod uticajem fluorantena, sa izraženijim odgovorima u tkivu srednjeg creva, kao i razlike u obrascima odgovora između vrsta. Uočeno je smanjenje ekspresije Hsp70 u celom srednjem crevu, a različiti odgovori Hsp70 u mozgu larvi. Kod obe vrste je došlo i do značajnih promena aktivnosti digestivnih enzima i njihovih izoformi u srednjem crevu larvi izlaganih fluorantenu. Detektovana je inhibicija aktivnosti određenih digestivnih enzima i njihovih izoformi kod larvi L. dispar, dok je kod larvi E. chrysorrhoea zabeleženo povećanje aktivnosti većeg broja enzima ove grupe, kao i ekspresija izoformi pojedinih enzima kod larvi izlaganih fluorantenu. Kanonijskom diskriminacionom analizom su potvrđene razlike između vrsta kada su u pitanju odgovori antioksidativnih i digestivnih enzima na prisustvo fluorantena u ishrani. Alokacija energetskih resursa ka mehanizmima odbrane, u prisustvu fluorantena, odrazila se na osobine životne istorije larvi obe vrste., Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbons, present in the leaves of various plants used for food by larvae of polyphagous insect species Lymantria dispar and Euproctis chrysorrhoea. The aim of dissertation was to gain insight into the patterns of physiological responses of L. dispar and E. chrysorrhoea larvae (Lepidoptera: Erebidae) to the presence of environmentally relevant concentrations of fluoranthene in food and to elucidate the mechanisms of defense against its harmful effects, which contributes to perceive the way they adjust to stressful environmental conditions. Chronic effects of fluoranthene (6.7 and 67 ng/g dry food weight) on L. dispar and E. chrysorrhoea larvae, originating from natural populations, were examined. Antioxidative and detoxification enzymes of midgut showed changes of activity under the influence of fluoranthene, with more pronounced responses in midgut tissue, and between-species differences in patterns of responses. Decreased expression of Hsp70 in the midgut, and different responses of Hsp70 in the brain of larvae were noticed. Significant changes of the activity of digestive enzymes and their isoforms in the midgut of larvae of both species exposed to fluoranthene were noticed. Exposure to fluorantene inhibited the activity of certain digestive enzymes and their isoforms of L. dispar larvae, and increased the activity of a number of enzymes and expression of isoforms of certain enzymes of E. chrysorrhoea larvae. Canonical discriminant analysis confirmed between-species differences in responses of antioxidative and digestive enzymes to the presence of fluoranthene in the diet. The allocation of energy resources to defense mechanisms affected the life history traits of treated larvae of both species.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena, Physiological adjustment of larvae of gypsy moth, Lymantria dispar L., and brown-tail moth, Euproctis chrysorrhoea L., (Lepidoptera) to the effects of fluoranthene",
pages = "1-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4668"
}

Filipović, A.. (2021). Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena. in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-118.
https://hdl.handle.net/21.15107/rcub_ibiss_4668

Filipović A. Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena. in Faculty of Biology, University of Belgrade. 2021;:1-118.
https://hdl.handle.net/21.15107/rcub_ibiss_4668 .

Filipović, Aleksandra, "Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena" in Faculty of Biology, University of Belgrade (2021):1-118,
https://hdl.handle.net/21.15107/rcub_ibiss_4668 .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4283
AB  - Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.
PB  - Elsevier
T2  - Environmntal Pollution
T1  - Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants
VL  - 288
DO  - 10.1016/j.envpol.2021.117706
SP  - 117706
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.",
publisher = "Elsevier",
journal = "Environmntal Pollution",
title = "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants",
volume = "288",
doi = "10.1016/j.envpol.2021.117706",
pages = "117706"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution
Elsevier., 288, 117706.
https://doi.org/10.1016/j.envpol.2021.117706

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution. 2021;288:117706.
doi:10.1016/j.envpol.2021.117706 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants" in Environmntal Pollution, 288 (2021):117706,
https://doi.org/10.1016/j.envpol.2021.117706 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3606
AB  - Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.
T2  - Ecological Indicators
T1  - Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment
VL  - 112
DO  - 10.1016/j.ecolind.2020.106136
SP  - 106136
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Mrdaković, Marija and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2020",
abstract = "Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.",
journal = "Ecological Indicators",
title = "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment",
volume = "112",
doi = "10.1016/j.ecolind.2020.106136",
pages = "106136"
}

Matić, D., Vlahović, M., Ilijin, L., Mrdaković, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2020). Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators, 112, 106136.
https://doi.org/10.1016/j.ecolind.2020.106136

Matić D, Vlahović M, Ilijin L, Mrdaković M, Grčić A, Filipović A, Perić Mataruga V. Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators. 2020;112:106136.
doi:10.1016/j.ecolind.2020.106136 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Mrdaković, Marija, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment" in Ecological Indicators, 112 (2020):106136,
https://doi.org/10.1016/j.ecolind.2020.106136 . .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Đurašević, Siniša
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4403
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3647
AB  - Plant vegetation accumulates polycyclic aromatic hydrocarbons (PAHs) among which benzo[a]pyrene (B[a]P) is recognized as being very toxic, including cancerogenic. Lymantria dispar L. larvae are sensitive to changes in the environment, providing potential signs of pollutant presence. We examined the chronic effects of two concentrations of B[a] P on the activity of carboxylesterase (CaE), acetylcholinesterase (AChE) and heat shock protein 70 (Hsp70) levels in the brain tissue of two populations of L. dispar larvae, originating from unpolluted and polluted habitats. We found that the relative growth rate was significantly lower in both populations and that only larvae from polluted forests were sensitive to low B[a]P concentrations, exhibiting a significant increase in brain tissue CaE activity and Hsp70 concentration. AChE activity showed no changes in response to B[a]P exposure in either population. Examined biochemical parameters indicate that their sensitivity to chronic treatment with B[a]P was highly dependent on the pre-exposure history of L. dispar larvae, suggesting that they could be promising biomarkers of B[a]P and PAH pollution in forest ecosystems
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests
IS  - 4
VL  - 71
DO  - 10.2298/abs190620056g
SP  - 735
EP  - 745
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Đurašević, Siniša and Perić Mataruga, Vesna",
year = "2019",
abstract = "Plant vegetation accumulates polycyclic aromatic hydrocarbons (PAHs) among which benzo[a]pyrene (B[a]P) is recognized as being very toxic, including cancerogenic. Lymantria dispar L. larvae are sensitive to changes in the environment, providing potential signs of pollutant presence. We examined the chronic effects of two concentrations of B[a] P on the activity of carboxylesterase (CaE), acetylcholinesterase (AChE) and heat shock protein 70 (Hsp70) levels in the brain tissue of two populations of L. dispar larvae, originating from unpolluted and polluted habitats. We found that the relative growth rate was significantly lower in both populations and that only larvae from polluted forests were sensitive to low B[a]P concentrations, exhibiting a significant increase in brain tissue CaE activity and Hsp70 concentration. AChE activity showed no changes in response to B[a]P exposure in either population. Examined biochemical parameters indicate that their sensitivity to chronic treatment with B[a]P was highly dependent on the pre-exposure history of L. dispar larvae, suggesting that they could be promising biomarkers of B[a]P and PAH pollution in forest ecosystems",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests",
number = "4",
volume = "71",
doi = "10.2298/abs190620056g",
pages = "735-745"
}

Grčić, A., Ilijin, L., Mrdaković, M., Vlahović, M., Filipović, A., Đurašević, S.,& Perić Mataruga, V.. (2019). Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests. in Archives of Biological Sciences
Serbian Biological Society., 71(4), 735-745.
https://doi.org/10.2298/abs190620056g

Grčić A, Ilijin L, Mrdaković M, Vlahović M, Filipović A, Đurašević S, Perić Mataruga V. Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests. in Archives of Biological Sciences. 2019;71(4):735-745.
doi:10.2298/abs190620056g .

Grčić, Anja, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Đurašević, Siniša, Perić Mataruga, Vesna, "Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests" in Archives of Biological Sciences, 71, no. 4 (2019):735-745,
https://doi.org/10.2298/abs190620056g . .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Grčić, Anja
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-019-04133-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3238
AB  - Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants which exert detrimental effects on living beings. Considering the health risk associated with exposure to these pollutants, their presence in food increases efforts to establish early-warning indicators of pollution. We aimed to examine the effects of environmentally relevant concentrations of fluoranthene (0.2 ng and 18 ng/g dry weight of diet) on the activities of midgut antioxidant and detoxification enzymes in Blaptica dubia. Significant changes of superoxide dismutase and catalase activities, recorded at the higher fluoranthene concentration regardless of the exposure time, suggest that they may be used as biomarkers of PAH pollution. Increased GST activity and decreased total GSH content, detected upon acute exposure to the lower concentration, indicate processes of detoxification. Reorganization of B. dubia mechanisms of defense in response to oxidative stress caused by exposure to dietary PAH point to the necessity for further examination of fluoranthene actions.
PB  - Springer Verlag
T2  - Environmental Science and Pollution Research
T1  - Effects of dietary fluoranthene on nymphs of Blaptica dubia S. (Blattodea: Blaberidae).
IS  - 6
VL  - 26
DO  - 10.1007/s11356-019-04133-1
SP  - 6216
EP  - 6222
ER  - 

@article{
author = "Mrdaković, Marija and Ilijin, Larisa and Vlahović, Milena and Filipović, Aleksandra and Grčić, Anja and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2019",
abstract = "Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants which exert detrimental effects on living beings. Considering the health risk associated with exposure to these pollutants, their presence in food increases efforts to establish early-warning indicators of pollution. We aimed to examine the effects of environmentally relevant concentrations of fluoranthene (0.2 ng and 18 ng/g dry weight of diet) on the activities of midgut antioxidant and detoxification enzymes in Blaptica dubia. Significant changes of superoxide dismutase and catalase activities, recorded at the higher fluoranthene concentration regardless of the exposure time, suggest that they may be used as biomarkers of PAH pollution. Increased GST activity and decreased total GSH content, detected upon acute exposure to the lower concentration, indicate processes of detoxification. Reorganization of B. dubia mechanisms of defense in response to oxidative stress caused by exposure to dietary PAH point to the necessity for further examination of fluoranthene actions.",
publisher = "Springer Verlag",
journal = "Environmental Science and Pollution Research",
title = "Effects of dietary fluoranthene on nymphs of Blaptica dubia S. (Blattodea: Blaberidae).",
number = "6",
volume = "26",
doi = "10.1007/s11356-019-04133-1",
pages = "6216-6222"
}

Mrdaković, M., Ilijin, L., Vlahović, M., Filipović, A., Grčić, A., Todorović, D.,& Perić Mataruga, V.. (2019). Effects of dietary fluoranthene on nymphs of Blaptica dubia S. (Blattodea: Blaberidae).. in Environmental Science and Pollution Research
Springer Verlag., 26(6), 6216-6222.
https://doi.org/10.1007/s11356-019-04133-1

Mrdaković M, Ilijin L, Vlahović M, Filipović A, Grčić A, Todorović D, Perić Mataruga V. Effects of dietary fluoranthene on nymphs of Blaptica dubia S. (Blattodea: Blaberidae).. in Environmental Science and Pollution Research. 2019;26(6):6216-6222.
doi:10.1007/s11356-019-04133-1 .

Mrdaković, Marija, Ilijin, Larisa, Vlahović, Milena, Filipović, Aleksandra, Grčić, Anja, Todorović, Dajana, Perić Mataruga, Vesna, "Effects of dietary fluoranthene on nymphs of Blaptica dubia S. (Blattodea: Blaberidae)." in Environmental Science and Pollution Research, 26, no. 6 (2019):6216-6222,
https://doi.org/10.1007/s11356-019-04133-1 . .

TY  - CONF
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Grčić, Anja
AU  - Perić Mataruga, Vesna
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3982
AB  - Policiklični aromatični ugljovodonici (PAH) su organske zagađujuće materije koje u najvećoj meri nastaju kao posledica antropogenih aktivnosti. PAH su široko rasprostranjeni u životnoj sredini, štetni efekti njihovog prisustva detektovani su na različitim nivoima biološke organizacije biljaka i životinja, a brojni su ispoljili toksično i kancerogeno delovanje na ljude. Fluoranten (Flann) je jedan od najrasprostranjenijih PAH, često indikator prisustva drugih jedinjenja ove grupe zagađivača i jedan od dominantnih u lišću listopadnih i četinarskih vrsta koje su biljke hraniteljke polifagnih vrsta insekata Lymantria dispar L. i Euproctis chrysorrhoea L. Cilj ispitivanja je bio ustanoviti aktivnost antioksidativnih enzima superoksid dismutaze (SOD) i katalaze (CAT) i ekspresiju njihovih izoformi, u homogenatima hemolimfe larvi L. dispar i E. chrysorrhoea izlaganih hroničnom delovanju sredinski relevantnih koncentracija Flann (6.7 i 67 ng/g suve mase veštačke hrane). Ekspresija izoformi SOD u hemolimfi larvi L. dispar, zavisna od koncentracije Flann, sugeriše efikasnu eliminaciju superoxid anjon radikala i pri smanjenoj aktivnosti enzima, dok značajno povećanje aktivnosti CAT ukazuje na protektivnu ulogu enzima u uslovima povećanja koncentracije H2O2. Aktivnost SOD u hemolimfi larvi E. chrysorrhoea se nije promenila, dok značajno redukovana aktivnost CAT može ukazivati na uključenje drugih mehanizama zaštite od toksičnog delovanja organskog zagađivača i produkata njegovog metabolizma.
PB  - Belgrade: Serbian Biological Society
C3  - Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 155
T1  - Delovanje fluorantena, policikličnog aromatičnog ugljovodonika, na aktivnost enzima superoksid dismutaze i katalaze u hemolimfi larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.)
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3982
ER  - 

@conference{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Vlahović, Milena and Todorović, Dajana and Grčić, Anja and Perić Mataruga, Vesna",
year = "2018",
abstract = "Policiklični aromatični ugljovodonici (PAH) su organske zagađujuće materije koje u najvećoj meri nastaju kao posledica antropogenih aktivnosti. PAH su široko rasprostranjeni u životnoj sredini, štetni efekti njihovog prisustva detektovani su na različitim nivoima biološke organizacije biljaka i životinja, a brojni su ispoljili toksično i kancerogeno delovanje na ljude. Fluoranten (Flann) je jedan od najrasprostranjenijih PAH, često indikator prisustva drugih jedinjenja ove grupe zagađivača i jedan od dominantnih u lišću listopadnih i četinarskih vrsta koje su biljke hraniteljke polifagnih vrsta insekata Lymantria dispar L. i Euproctis chrysorrhoea L. Cilj ispitivanja je bio ustanoviti aktivnost antioksidativnih enzima superoksid dismutaze (SOD) i katalaze (CAT) i ekspresiju njihovih izoformi, u homogenatima hemolimfe larvi L. dispar i E. chrysorrhoea izlaganih hroničnom delovanju sredinski relevantnih koncentracija Flann (6.7 i 67 ng/g suve mase veštačke hrane). Ekspresija izoformi SOD u hemolimfi larvi L. dispar, zavisna od koncentracije Flann, sugeriše efikasnu eliminaciju superoxid anjon radikala i pri smanjenoj aktivnosti enzima, dok značajno povećanje aktivnosti CAT ukazuje na protektivnu ulogu enzima u uslovima povećanja koncentracije H2O2. Aktivnost SOD u hemolimfi larvi E. chrysorrhoea se nije promenila, dok značajno redukovana aktivnost CAT može ukazivati na uključenje drugih mehanizama zaštite od toksičnog delovanja organskog zagađivača i produkata njegovog metabolizma.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 155",
title = "Delovanje fluorantena, policikličnog aromatičnog ugljovodonika, na aktivnost enzima superoksid dismutaze i katalaze u hemolimfi larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.)",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3982"
}

Filipović, A., Mrdaković, M., Ilijin, L., Vlahović, M., Todorović, D., Grčić, A.,& Perić Mataruga, V.. (2018). Delovanje fluorantena, policikličnog aromatičnog ugljovodonika, na aktivnost enzima superoksid dismutaze i katalaze u hemolimfi larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.). in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 155
Belgrade: Serbian Biological Society., 155.
https://hdl.handle.net/21.15107/rcub_ibiss_3982

Filipović A, Mrdaković M, Ilijin L, Vlahović M, Todorović D, Grčić A, Perić Mataruga V. Delovanje fluorantena, policikličnog aromatičnog ugljovodonika, na aktivnost enzima superoksid dismutaze i katalaze u hemolimfi larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.). in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 155. 2018;:155.
https://hdl.handle.net/21.15107/rcub_ibiss_3982 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Vlahović, Milena, Todorović, Dajana, Grčić, Anja, Perić Mataruga, Vesna, "Delovanje fluorantena, policikličnog aromatičnog ugljovodonika, na aktivnost enzima superoksid dismutaze i katalaze u hemolimfi larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.)" in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 155 (2018):155,
https://hdl.handle.net/21.15107/rcub_ibiss_3982 .

TY  - CONF
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3962
AB  - Razvoj industrije je tokom poslednjih decenija doveo do povećanja koncentracije izuzetno toksičnog teškog metala kadmijuma u životnoj sredini, što je uslovilo potrebu za stalnim unapređivanjem biomonitoringa. Kod insekata se oralno uneti kadmijum akumulira primarno u crevu, gde utiče na aktivnost digestivnih enzima. Cilj ove studije bio je proceniti specifične aktivnosti tripsina i leucin aminopeptidaze (LAP) i detektovati njihove izoforme u srednjem crevu larvi Lymantria dispar kao potencijalne biomarkere zagađenosti kadmijumom u zavisnosti od porekla populacije. Legla gubara sakupljena su na dve lokacije – u nezagađenoj šumi na planini Kosmaj, 60 km od Beograda, i na zagađenoj lokaciji uz Ibarsku magistralu u Beogradu. Jedinke su hronično bile izložene koncentracijama od 50 i 100 μg Cd/g suve hrane od izleganja do četvrtog stupnja, kada su žrtvovane. U populaciji sa Kosmaja oba tretmana su dovela do sniženja aktivnosti tripsina i LAP, pri čemu je aktivnost LAP na višoj koncentraciji metala bila značajno viša u odnosu na nižu koncentraciju. Kod larvi sa zagađenog lokaliteta zabeležen je samo rast aktivnosti LAP nakon tretmana sa 50 μg Cd/g suve hrane. Tri izoforme tripsina i šest izoformi LAP detektovano je u obe populacije u različitom broju i intenzitetu zavisno od koncentracije kadmijuma. Rezultati sugerišu da bi parametri aktivnosti tripsina mogli biti adekvatni biomarkeri kontaminacije kadmijumom u populacijama koje prethodno nisu bile izložene zagađujućim supstancama.
PB  - Belgrade: Serbian Biological Society
C3  - Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156
T1  - Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu
SP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3962
ER  - 

@conference{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Mrdaković, Marija and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2018",
abstract = "Razvoj industrije je tokom poslednjih decenija doveo do povećanja koncentracije izuzetno toksičnog teškog metala kadmijuma u životnoj sredini, što je uslovilo potrebu za stalnim unapređivanjem biomonitoringa. Kod insekata se oralno uneti kadmijum akumulira primarno u crevu, gde utiče na aktivnost digestivnih enzima. Cilj ove studije bio je proceniti specifične aktivnosti tripsina i leucin aminopeptidaze (LAP) i detektovati njihove izoforme u srednjem crevu larvi Lymantria dispar kao potencijalne biomarkere zagađenosti kadmijumom u zavisnosti od porekla populacije. Legla gubara sakupljena su na dve lokacije – u nezagađenoj šumi na planini Kosmaj, 60 km od Beograda, i na zagađenoj lokaciji uz Ibarsku magistralu u Beogradu. Jedinke su hronično bile izložene koncentracijama od 50 i 100 μg Cd/g suve hrane od izleganja do četvrtog stupnja, kada su žrtvovane. U populaciji sa Kosmaja oba tretmana su dovela do sniženja aktivnosti tripsina i LAP, pri čemu je aktivnost LAP na višoj koncentraciji metala bila značajno viša u odnosu na nižu koncentraciju. Kod larvi sa zagađenog lokaliteta zabeležen je samo rast aktivnosti LAP nakon tretmana sa 50 μg Cd/g suve hrane. Tri izoforme tripsina i šest izoformi LAP detektovano je u obe populacije u različitom broju i intenzitetu zavisno od koncentracije kadmijuma. Rezultati sugerišu da bi parametri aktivnosti tripsina mogli biti adekvatni biomarkeri kontaminacije kadmijumom u populacijama koje prethodno nisu bile izložene zagađujućim supstancama.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156",
title = "Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3962"
}

Matić, D., Vlahović, M., Ilijin, L., Mrdaković, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2018). Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu. in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156
Belgrade: Serbian Biological Society., 156.
https://hdl.handle.net/21.15107/rcub_ibiss_3962

Matić D, Vlahović M, Ilijin L, Mrdaković M, Grčić A, Filipović A, Perić Mataruga V. Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu. in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156. 2018;:156.
https://hdl.handle.net/21.15107/rcub_ibiss_3962 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Mrdaković, Marija, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Tripsin i leucin aminopeptidaza srednjeg creva larvi gubara (Lymantria dispar L.) kao potencijalni biomarkeri hroničnog izlaganja kadmijumu" in Miroslav Živić, Branka Petković, urednici. Drugi kongres biologa Srbije - osnovna i primenjena istrazivanja, metodika nastave; 2018 Sep 25-30; Kladovo, Srbija; Knjiga sažetaka, str. 156 (2018):156,
https://hdl.handle.net/21.15107/rcub_ibiss_3962 .

TY  - GEN
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2017
UR  - http://link.springer.com/10.1007/s11356-017-9898-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2834
AB  - The effects of different concentrations of benzo(a)pyrene (BaP) (2, 10, 20, 100, 200, and 2000 ng/g dry food weight) on the activity of acetylcholinesterase (AChE) and concentration of heat-shock protein 70 (hsp70) in fifth instar Lymantria dispar brain tissue were investigated. Inhibition of AChE is a general effect biomarker at the individual level. We observed the most efficient inhibition of this enzyme at medium and high BaP concentrations (20, 100, 200, and 2000 ng/g dry food weight). Western blot analysis revealed the presence of two hsp 70 isoforms, with changed amounts depending on the BaP concentration. A fluctuating response in hsp70 concentration to ingested BaP was observed: with decreased levels in the groups fed 2 and 100 ng BaP/g dry food weight, but increased concentrations in the groups given 10 and 2000 ng/g dry food weight. Even though we detected changes in AChE activity and hsp70 concentration in L. dispar brain tissue upon exposure to different BaP concentrations, we cannot characterize them as sensitive and reliable biomarkers for this xenobiotic in L. dispar caterpillars.
PB  - Springer Verlag
T2  - Environmental Science and Pollution Research
T1  - Acetylcholinesterase and heat shock protein 70 response in larval brain tissue of Lymantria dispar L. (Lepidoptera, Limantriidae) upon chronic exposure to benzo(a)pyrene
IS  - 25
VL  - 24
DO  - 10.1007/s11356-017-9898-0
SP  - 20818
EP  - 20823
ER  - 

@misc{
author = "Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2017",
abstract = "The effects of different concentrations of benzo(a)pyrene (BaP) (2, 10, 20, 100, 200, and 2000 ng/g dry food weight) on the activity of acetylcholinesterase (AChE) and concentration of heat-shock protein 70 (hsp70) in fifth instar Lymantria dispar brain tissue were investigated. Inhibition of AChE is a general effect biomarker at the individual level. We observed the most efficient inhibition of this enzyme at medium and high BaP concentrations (20, 100, 200, and 2000 ng/g dry food weight). Western blot analysis revealed the presence of two hsp 70 isoforms, with changed amounts depending on the BaP concentration. A fluctuating response in hsp70 concentration to ingested BaP was observed: with decreased levels in the groups fed 2 and 100 ng BaP/g dry food weight, but increased concentrations in the groups given 10 and 2000 ng/g dry food weight. Even though we detected changes in AChE activity and hsp70 concentration in L. dispar brain tissue upon exposure to different BaP concentrations, we cannot characterize them as sensitive and reliable biomarkers for this xenobiotic in L. dispar caterpillars.",
publisher = "Springer Verlag",
journal = "Environmental Science and Pollution Research",
title = "Acetylcholinesterase and heat shock protein 70 response in larval brain tissue of Lymantria dispar L. (Lepidoptera, Limantriidae) upon chronic exposure to benzo(a)pyrene",
number = "25",
volume = "24",
doi = "10.1007/s11356-017-9898-0",
pages = "20818-20823"
}

Ilijin, L., Mrdaković, M., Vlahović, M., Matić, D., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2017). Acetylcholinesterase and heat shock protein 70 response in larval brain tissue of Lymantria dispar L. (Lepidoptera, Limantriidae) upon chronic exposure to benzo(a)pyrene. in Environmental Science and Pollution Research
Springer Verlag., 24(25), 20818-20823.
https://doi.org/10.1007/s11356-017-9898-0

Ilijin L, Mrdaković M, Vlahović M, Matić D, Grčić A, Filipović A, Perić Mataruga V. Acetylcholinesterase and heat shock protein 70 response in larval brain tissue of Lymantria dispar L. (Lepidoptera, Limantriidae) upon chronic exposure to benzo(a)pyrene. in Environmental Science and Pollution Research. 2017;24(25):20818-20823.
doi:10.1007/s11356-017-9898-0 .

Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Acetylcholinesterase and heat shock protein 70 response in larval brain tissue of Lymantria dispar L. (Lepidoptera, Limantriidae) upon chronic exposure to benzo(a)pyrene" in Environmental Science and Pollution Research, 24, no. 25 (2017):20818-20823,
https://doi.org/10.1007/s11356-017-9898-0 . .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0045653517304526
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2727
AB  - Anthropogenic activity in industrial development has imposed great threats to the environment and wildlife in the form of persistent organic pollutants. Polycyclic aromatic hydrocarbons (PAH) tend to accumulate in vegetation foliage which is the main food source of polyphagous insect species Lymantria dispar L. Origin and multigenerational adaptation of L. dispar population to environmental challenges strongly condition the enzymes’ sensitivity to pollutants. In this study, our aim was to investigate response of the superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) to the chronic dietary exposure of benzo[a]pyrene in the midgut tissues and hemolymph of two L. dispar populations originating from unpolluted and polluted forest habitat. Midgut tissue of the larvae from the polluted forest showed significant increase in SOD, CAT and GST activity, while in unpolluted forest's larvae SOD and CAT showed elevated activities in hemolymph. L. dispar populations adapted to different level of pollution in their environment and expressed distinct tissue-dependent antioxidative enzyme sensitivity to benzo[a]pyrene diet, implying high potential for further elucidation of these enzymes as molecular biomarkers.
PB  - Elsevier
T2  - Chemosphere
T1  - Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae ) larvae from unpolluted and polluted forests
VL  - 179
DO  - 10.1016/j.chemosphere.2017.03.083
SP  - 10
EP  - 19
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2017",
abstract = "Anthropogenic activity in industrial development has imposed great threats to the environment and wildlife in the form of persistent organic pollutants. Polycyclic aromatic hydrocarbons (PAH) tend to accumulate in vegetation foliage which is the main food source of polyphagous insect species Lymantria dispar L. Origin and multigenerational adaptation of L. dispar population to environmental challenges strongly condition the enzymes’ sensitivity to pollutants. In this study, our aim was to investigate response of the superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) to the chronic dietary exposure of benzo[a]pyrene in the midgut tissues and hemolymph of two L. dispar populations originating from unpolluted and polluted forest habitat. Midgut tissue of the larvae from the polluted forest showed significant increase in SOD, CAT and GST activity, while in unpolluted forest's larvae SOD and CAT showed elevated activities in hemolymph. L. dispar populations adapted to different level of pollution in their environment and expressed distinct tissue-dependent antioxidative enzyme sensitivity to benzo[a]pyrene diet, implying high potential for further elucidation of these enzymes as molecular biomarkers.",
publisher = "Elsevier",
journal = "Chemosphere",
title = "Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae ) larvae from unpolluted and polluted forests",
volume = "179",
doi = "10.1016/j.chemosphere.2017.03.083",
pages = "10-19"
}

Grčić, A., Ilijin, L., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2017). Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae ) larvae from unpolluted and polluted forests. in Chemosphere
Elsevier., 179, 10-19.
https://doi.org/10.1016/j.chemosphere.2017.03.083

Grčić A, Ilijin L, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae ) larvae from unpolluted and polluted forests. in Chemosphere. 2017;179:10-19.
doi:10.1016/j.chemosphere.2017.03.083 .

Grčić, Anja, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae ) larvae from unpolluted and polluted forests" in Chemosphere, 179 (2017):10-19,
https://doi.org/10.1016/j.chemosphere.2017.03.083 . .