TY  - JOUR
AU  - Todorović, Smilja
AU  - Simeunović, Valentina
AU  - Prvulović, Milica
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Sokanović, Srđan
AU  - Kanazir, Selma
AU  - Mladenović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6373
AB  - Insulin is known to be a key hormone in the regulation of peripheral glucose homeostasis, but beyond that, its effects on the brain are now undisputed. Impairments in insulin signaling in the brain, including changes in insulin levels, are thought to contribute significantly to declines in cognitive performance, especially during aging. As one of the most widely studied experimental interventions, dietary restriction (DR) is considered to delay the neurodegenerative processes associated with aging. Recently, however, data began to suggest that the onset and duration of a restrictive diet play a critical role in the putative beneficial outcome. Because the effects of DR on insulin signaling in the brain have been poorly studied, we decided to examine the effects of DR that differed in onset and duration: long-term DR (LTDR), medium-term DR (MTDR), and short-term DR (STDR) on the expression of proteins involved in insulin signaling in the hippocampus of 18- and 24-month-old male Wistar rats. We found that DR-induced changes in insulin levels in the brain may be independent of what happens in the periphery after restricted feeding. Significantly changed insulin content in the hippocampus, together with altered insulin signaling were found under the influence of DR, but the outcome was highly dependent on the onset and duration of DR.
PB  - Hoboken: John Wiley and Sons
T2  - Biofactors
T1  - Dietary restriction alters insulin signaling pathway in the brain
DO  - 10.1002/biof.2018
ER  - 

@article{
author = "Todorović, Smilja and Simeunović, Valentina and Prvulović, Milica and Dakić, Tamara and Jevđović, Tanja and Sokanović, Srđan and Kanazir, Selma and Mladenović, Aleksandra",
year = "2023",
abstract = "Insulin is known to be a key hormone in the regulation of peripheral glucose homeostasis, but beyond that, its effects on the brain are now undisputed. Impairments in insulin signaling in the brain, including changes in insulin levels, are thought to contribute significantly to declines in cognitive performance, especially during aging. As one of the most widely studied experimental interventions, dietary restriction (DR) is considered to delay the neurodegenerative processes associated with aging. Recently, however, data began to suggest that the onset and duration of a restrictive diet play a critical role in the putative beneficial outcome. Because the effects of DR on insulin signaling in the brain have been poorly studied, we decided to examine the effects of DR that differed in onset and duration: long-term DR (LTDR), medium-term DR (MTDR), and short-term DR (STDR) on the expression of proteins involved in insulin signaling in the hippocampus of 18- and 24-month-old male Wistar rats. We found that DR-induced changes in insulin levels in the brain may be independent of what happens in the periphery after restricted feeding. Significantly changed insulin content in the hippocampus, together with altered insulin signaling were found under the influence of DR, but the outcome was highly dependent on the onset and duration of DR.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Biofactors",
title = "Dietary restriction alters insulin signaling pathway in the brain",
doi = "10.1002/biof.2018"
}

Todorović, S., Simeunović, V., Prvulović, M., Dakić, T., Jevđović, T., Sokanović, S., Kanazir, S.,& Mladenović, A.. (2023). Dietary restriction alters insulin signaling pathway in the brain. in Biofactors
Hoboken: John Wiley and Sons..
https://doi.org/10.1002/biof.2018

Todorović S, Simeunović V, Prvulović M, Dakić T, Jevđović T, Sokanović S, Kanazir S, Mladenović A. Dietary restriction alters insulin signaling pathway in the brain. in Biofactors. 2023;.
doi:10.1002/biof.2018 .

Todorović, Smilja, Simeunović, Valentina, Prvulović, Milica, Dakić, Tamara, Jevđović, Tanja, Sokanović, Srđan, Kanazir, Selma, Mladenović, Aleksandra, "Dietary restriction alters insulin signaling pathway in the brain" in Biofactors (2023),
https://doi.org/10.1002/biof.2018 . .

TY  - JOUR
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Vujović, Predrag
AU  - Mladenović, Aleksandra
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/12/6546
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5018
AB  - Striving for longevity is neither a recent human desire nor a novel scientific field. The first article on this topic was published in 1838, when the average human life expectancy was approximately 40 years. Although nowadays people on average live almost as twice as long, we still (and perhaps more than ever) look for new ways to extend our lifespan. During this seemingly endless journey of discovering efficient methods to prolong life, humans were enthusiastic regarding several approaches, one of which is caloric restriction (CR). Where does CR, initially considered universally beneficial for extending both lifespan and health span, stand today? Does a lifelong decrease in food consumption represent one of the secrets of centenarians' long and healthy life? Do we still believe that if we eat less, we will live longer? This review aims to summarize the current literature on CR as a potential life-prolonging intervention in humans and discusses metabolic pathways that underlie this effect.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?
IS  - 12
VL  - 23
DO  - 10.3390/ijms23126546
SP  - 6546
ER  - 

@article{
author = "Dakić, Tamara and Jevđović, Tanja and Vujović, Predrag and Mladenović, Aleksandra",
year = "2022",
abstract = "Striving for longevity is neither a recent human desire nor a novel scientific field. The first article on this topic was published in 1838, when the average human life expectancy was approximately 40 years. Although nowadays people on average live almost as twice as long, we still (and perhaps more than ever) look for new ways to extend our lifespan. During this seemingly endless journey of discovering efficient methods to prolong life, humans were enthusiastic regarding several approaches, one of which is caloric restriction (CR). Where does CR, initially considered universally beneficial for extending both lifespan and health span, stand today? Does a lifelong decrease in food consumption represent one of the secrets of centenarians' long and healthy life? Do we still believe that if we eat less, we will live longer? This review aims to summarize the current literature on CR as a potential life-prolonging intervention in humans and discusses metabolic pathways that underlie this effect.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?",
number = "12",
volume = "23",
doi = "10.3390/ijms23126546",
pages = "6546"
}

Dakić, T., Jevđović, T., Vujović, P.,& Mladenović, A.. (2022). The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?. in International Journal of Molecular Sciences
Basel: MDPI., 23(12), 6546.
https://doi.org/10.3390/ijms23126546

Dakić T, Jevđović T, Vujović P, Mladenović A. The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?. in International Journal of Molecular Sciences. 2022;23(12):6546.
doi:10.3390/ijms23126546 .

Dakić, Tamara, Jevđović, Tanja, Vujović, Predrag, Mladenović, Aleksandra, "The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?" in International Journal of Molecular Sciences, 23, no. 12 (2022):6546,
https://doi.org/10.3390/ijms23126546 . .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Bogdanović, Ljiljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Jasnić, Nebojša
AU  - Vujović, Predrag
AU  - Dakić, Tamara
AU  - Todorović, Zoran
AU  - Đorđević, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6333
AB  - Acute renal ischemia/reperfusion (I/R) is a temporary restriction of kidney blood supply, followed by blood flow restoration and re-oxygenation. During I/R, decreased oxygen supply disturbs ion transport, intracellular ATP, calcium and pH levels, and numerous signalling pathways. Upon reperfusion, a restoration of oxygen level rises a reactive oxygen species generation, cytokines and chemokines release from activated tissue-resident macrophages, and infiltration of pro-inflammatory neutrophils into ischemic tissues. All these changes result in cell swelling and rupturing, and consequent necrotic or apoptotic cell death. Meldonium is an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, which acts by shifting energy production from fatty acid oxidation to glycolysis. We investigated the effects of a 4-week meldonium pre-treatment with 300 mg/kg b.m./day of rats subjected to a well-established experimental model of renal I/R, with ischemia lasting for 45 minutes, followed by 4 hours of reperfusion. The degree of apoptosis and necrosis was evaluated by measuring renal pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, serum and kidney levels of necrotic marker - high mobility group box 1 protein (HMGB1), together with the kidney histology analysis. Our results showed that apoptotic and necrotic cell death occur simultaneously under I/R conditions, judging by the renal Bax/Bcl2 ratio rise (2.7-fold), increase in serum (22%) and renal (30%) levels of HMGB1, as well as severe tubular necrosis with dilatation of the tubular structure, cast formation, tubular lumina dilatation, brush border reduction, and loss in some renal areas cells. Meldonium pretreatment reduced the elevated Bax/Bcl2 ratio by 35%, as well as the serum and renal HMGB1 levels by 20% and notably diminished histological evidence of renal I/R necrotic injury, especially regarding tubular structures. These findings proved that meldonium protects renal cells against I/R-induced necrosis and apoptosis.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches
T1  - Meldonium prevents acute ishemia/reperfusion inducend-renal cells death in rats
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6333
ER  - 

@conference{
author = "Đurašević, Siniša and Stojković, Maja and Bogdanović, Ljiljana and Grigorov, Ilijana and Bogojević, Desanka and Jasnić, Nebojša and Vujović, Predrag and Dakić, Tamara and Todorović, Zoran and Đorđević, Jelena",
year = "2019",
abstract = "Acute renal ischemia/reperfusion (I/R) is a temporary restriction of kidney blood supply, followed by blood flow restoration and re-oxygenation. During I/R, decreased oxygen supply disturbs ion transport, intracellular ATP, calcium and pH levels, and numerous signalling pathways. Upon reperfusion, a restoration of oxygen level rises a reactive oxygen species generation, cytokines and chemokines release from activated tissue-resident macrophages, and infiltration of pro-inflammatory neutrophils into ischemic tissues. All these changes result in cell swelling and rupturing, and consequent necrotic or apoptotic cell death. Meldonium is an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, which acts by shifting energy production from fatty acid oxidation to glycolysis. We investigated the effects of a 4-week meldonium pre-treatment with 300 mg/kg b.m./day of rats subjected to a well-established experimental model of renal I/R, with ischemia lasting for 45 minutes, followed by 4 hours of reperfusion. The degree of apoptosis and necrosis was evaluated by measuring renal pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, serum and kidney levels of necrotic marker - high mobility group box 1 protein (HMGB1), together with the kidney histology analysis. Our results showed that apoptotic and necrotic cell death occur simultaneously under I/R conditions, judging by the renal Bax/Bcl2 ratio rise (2.7-fold), increase in serum (22%) and renal (30%) levels of HMGB1, as well as severe tubular necrosis with dilatation of the tubular structure, cast formation, tubular lumina dilatation, brush border reduction, and loss in some renal areas cells. Meldonium pretreatment reduced the elevated Bax/Bcl2 ratio by 35%, as well as the serum and renal HMGB1 levels by 20% and notably diminished histological evidence of renal I/R necrotic injury, especially regarding tubular structures. These findings proved that meldonium protects renal cells against I/R-induced necrosis and apoptosis.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches",
title = "Meldonium prevents acute ishemia/reperfusion inducend-renal cells death in rats",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6333"
}

Đurašević, S., Stojković, M., Bogdanović, L., Grigorov, I., Bogojević, D., Jasnić, N., Vujović, P., Dakić, T., Todorović, Z.,& Đorđević, J.. (2019). Meldonium prevents acute ishemia/reperfusion inducend-renal cells death in rats. in Immunology at the Confluence of Multidisciplinary Approaches
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 86.
https://hdl.handle.net/21.15107/rcub_ibiss_6333

Đurašević S, Stojković M, Bogdanović L, Grigorov I, Bogojević D, Jasnić N, Vujović P, Dakić T, Todorović Z, Đorđević J. Meldonium prevents acute ishemia/reperfusion inducend-renal cells death in rats. in Immunology at the Confluence of Multidisciplinary Approaches. 2019;:86.
https://hdl.handle.net/21.15107/rcub_ibiss_6333 .

Đurašević, Siniša, Stojković, Maja, Bogdanović, Ljiljana, Grigorov, Ilijana, Bogojević, Desanka, Jasnić, Nebojša, Vujović, Predrag, Dakić, Tamara, Todorović, Zoran, Đorđević, Jelena, "Meldonium prevents acute ishemia/reperfusion inducend-renal cells death in rats" in Immunology at the Confluence of Multidisciplinary Approaches (2019):86,
https://hdl.handle.net/21.15107/rcub_ibiss_6333 .

TY  - JOUR
AU  - Dakić, Tamara B.
AU  - Jevđović, Tanja V.
AU  - Perić, Mina I.
AU  - Bjelobaba, Ivana
AU  - Markelić, Milica B.
AU  - Milutinović, Bojana S.
AU  - Lakić, Iva V.
AU  - Jasnić, Nebojša I.
AU  - Đorđević, Jelena D.
AU  - Vujović, Predrag Z.
PY  - 2017
UR  - http://doi.wiley.com/10.1111/ejn.13607
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2779
AB  - In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.
T2  - European Journal of Neuroscience
T1  - Short-term fasting promotes insulin expression in rat hypothalamus
IS  - 1
VL  - 46
DO  - 10.1111/ejn.13607
SP  - 1730
EP  - 1737
ER  - 

@article{
author = "Dakić, Tamara B. and Jevđović, Tanja V. and Perić, Mina I. and Bjelobaba, Ivana and Markelić, Milica B. and Milutinović, Bojana S. and Lakić, Iva V. and Jasnić, Nebojša I. and Đorđević, Jelena D. and Vujović, Predrag Z.",
year = "2017",
abstract = "In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.",
journal = "European Journal of Neuroscience",
title = "Short-term fasting promotes insulin expression in rat hypothalamus",
number = "1",
volume = "46",
doi = "10.1111/ejn.13607",
pages = "1730-1737"
}

Dakić, T. B., Jevđović, T. V., Perić, M. I., Bjelobaba, I., Markelić, M. B., Milutinović, B. S., Lakić, I. V., Jasnić, N. I., Đorđević, J. D.,& Vujović, P. Z.. (2017). Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience, 46(1), 1730-1737.
https://doi.org/10.1111/ejn.13607

Dakić TB, Jevđović TV, Perić MI, Bjelobaba I, Markelić MB, Milutinović BS, Lakić IV, Jasnić NI, Đorđević JD, Vujović PZ. Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience. 2017;46(1):1730-1737.
doi:10.1111/ejn.13607 .

Dakić, Tamara B., Jevđović, Tanja V., Perić, Mina I., Bjelobaba, Ivana, Markelić, Milica B., Milutinović, Bojana S., Lakić, Iva V., Jasnić, Nebojša I., Đorđević, Jelena D., Vujović, Predrag Z., "Short-term fasting promotes insulin expression in rat hypothalamus" in European Journal of Neuroscience, 46, no. 1 (2017):1730-1737,
https://doi.org/10.1111/ejn.13607 . .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3422
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3422
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis",
pages = "167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3422"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):167,
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3423
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3423
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3423"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):168,
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .