TY  - CONF
AU  - Božić, Iva
AU  - Savić, Danijela
AU  - Laketa, Danijela
AU  - Nedeljković, Nadežda
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6059
AB  - Microglial cells are immune cells of the central nervous system (CNS) that
play a major role in its surveillance. Changes in CNS homeostasis, invading
pathogens or neuron impairment, lead to activation of microglial cells that
quickly proliferate, acquire amoeboid morphology and produce toxic
mediators such as nitric oxide (NO) and pro-inflammatory cytokines. These
changes are regulated by transcription factors, most importantly NF-κB.
Although microglial activation is important for maintaining tissue
homeostasis, excessive activation leads to chronic inflammation that can
damage healthy neurons. Substances that suppress microglial activation are
potential therapeutics for neurodegenerative diseases. Benfotiamine (Sbenzoylthiamine-
O-monophosphate) is a synthetic derivative of vitamin B1
that has anti-inflammatory properties. In this study we investigated antiinflammatory
properties of benfotiamine on activated microglia in vitro.
BV-2 microglia were pre-treated with benfotiamine, stimulated with LPS
and their viability and morphology were evaluated. LPS induced prominent
alterations in cell morphology, enlargement of cell bodies and spreading of
multiple processes. Pre-treatment with benfotiamine before LPS
stimulation suppressed these morphological changes. Additionally,
benfotiamine diminished LPS induced NO production, without altering cell
viability. Furthermore, benfotiamine decreased LPS induced production of
pro-inflammatory cytokines TNF- α and IL-6, while increasing production
of anti-inflammatory cytokine IL-10. Analysis of NF-κB activation
revealed that benfotiamine's effects were mediated by this transcription
factor. In conclusion, benfotiamine exerts anti-inflammatory properties in LPS
activated microglia in vitro and should be further investigated as a potential
therapeutic for neurodegenerative diseases.
PB  - Belgrade: Immunological Society of Serbia
C3  - 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia
T1  - Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6059
ER  - 

@conference{
editor = "Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko",
author = "Božić, Iva and Savić, Danijela and Laketa, Danijela and Nedeljković, Nadežda and Peković, Sanja and Lavrnja, Irena",
year = "2015",
abstract = "Microglial cells are immune cells of the central nervous system (CNS) that
play a major role in its surveillance. Changes in CNS homeostasis, invading
pathogens or neuron impairment, lead to activation of microglial cells that
quickly proliferate, acquire amoeboid morphology and produce toxic
mediators such as nitric oxide (NO) and pro-inflammatory cytokines. These
changes are regulated by transcription factors, most importantly NF-κB.
Although microglial activation is important for maintaining tissue
homeostasis, excessive activation leads to chronic inflammation that can
damage healthy neurons. Substances that suppress microglial activation are
potential therapeutics for neurodegenerative diseases. Benfotiamine (Sbenzoylthiamine-
O-monophosphate) is a synthetic derivative of vitamin B1
that has anti-inflammatory properties. In this study we investigated antiinflammatory
properties of benfotiamine on activated microglia in vitro.
BV-2 microglia were pre-treated with benfotiamine, stimulated with LPS
and their viability and morphology were evaluated. LPS induced prominent
alterations in cell morphology, enlargement of cell bodies and spreading of
multiple processes. Pre-treatment with benfotiamine before LPS
stimulation suppressed these morphological changes. Additionally,
benfotiamine diminished LPS induced NO production, without altering cell
viability. Furthermore, benfotiamine decreased LPS induced production of
pro-inflammatory cytokines TNF- α and IL-6, while increasing production
of anti-inflammatory cytokine IL-10. Analysis of NF-κB activation
revealed that benfotiamine's effects were mediated by this transcription
factor. In conclusion, benfotiamine exerts anti-inflammatory properties in LPS
activated microglia in vitro and should be further investigated as a potential
therapeutic for neurodegenerative diseases.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia",
title = "Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6059"
}

Lukić, M., Jonjic, S., Nikolich-Zugich, J., Božić, I., Savić, D., Laketa, D., Nedeljković, N., Peković, S.,& Lavrnja, I.. (2015). Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia
Belgrade: Immunological Society of Serbia., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_6059

Lukić M, Jonjic S, Nikolich-Zugich J, Božić I, Savić D, Laketa D, Nedeljković N, Peković S, Lavrnja I. Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia. 2015;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_6059 .

Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko, Božić, Iva, Savić, Danijela, Laketa, Danijela, Nedeljković, Nadežda, Peković, Sanja, Lavrnja, Irena, "Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling" in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia (2015):71,
https://hdl.handle.net/21.15107/rcub_ibiss_6059 .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Grigorov, Ilijana
AU  - Petrović, Anja
AU  - Zolotarevski, Lidija
AU  - Nikolic, Milica
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://www.medf.kg.ac.rs/efis/Arandjelovac%20Abstract%20book%202015.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4811
AB  - Gastrointestinal (GI) tract is one of the main targets of cadmium (Cd),
important food and drinking water contaminant. In this study, the effect of
subchronic (30 days) oral (in drinking water) intake of environmentally
relevant doses of cadmium (5µg/ml and 50µg/ml) on intestinal [(tissue of
duodenum and mesenteric lymph node (mLN) cells)] was examined in
Dark Agouti (DA) rats. Atomic absorption spectrophotometry (AAS)
analysis revealed significant cadmium load in duodenum,which was
associated with changes of both intestinal bacterial load and composition
(Denaturing Gradient Gel Electrophoresis/DGGE).Shortening of villi,
damage to epithelium, increases in goblet-like vacuoles and mononuclear
cell infiltration in lamina propria were histologically evident at both
cadmium doses, with massive necrosis at higher Cd dose (judging by High
Mobility Group Box-1/HMGB-1 Western blot analysis).Increased levels of
proinflammatory cytokines (IL-1β, IFN-γ, IL-17) were observed at both Cd
doses (TNFα at higher dose solely). Cadmium administration affected
draining lymph nodes as well, judging by signs of stress response (drop of
cellular reduced glutathione/GSH at higher dose, rise of
metallothionein/MT mRNA at both doses).Increased cellularity of mLN
was observed at higher Cd dose, but with no changes in proliferative
responses. Intake of both Cd doses resulted in higher (compared to controls)
levels of IFN-γ and IL-17 mRNA as well as increased mLN cell
responsiveness to ConA stimulation.Significant increases in numbers of
CD68+ cells and stimulation of innate immune activities (numbers of
dihydrorhodamine/DHR+ cells and intracellular myeloperoxidase/MPO+
cells, LPS-stimulated nitric oxide/NO production and ex vivo IL-1β
expression) were observed at higher dose of cadmium.Proinflammatory
effects of cadmium might have resulted from changes in gut microbiota and
tissue damage.Interactions of this metal with intestinal immune system
should be taken into account when assessing dietary cadmium as health risk
factor.
PB  - Belgrade: Immunological Society of Serbia
C3  - 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia
T1  - Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4811
ER  - 

@conference{
editor = "Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko",
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Mileusnić, Dina and Grigorov, Ilijana and Petrović, Anja and Zolotarevski, Lidija and Nikolic, Milica and Kataranovski, Milena",
year = "2015",
abstract = "Gastrointestinal (GI) tract is one of the main targets of cadmium (Cd),
important food and drinking water contaminant. In this study, the effect of
subchronic (30 days) oral (in drinking water) intake of environmentally
relevant doses of cadmium (5µg/ml and 50µg/ml) on intestinal [(tissue of
duodenum and mesenteric lymph node (mLN) cells)] was examined in
Dark Agouti (DA) rats. Atomic absorption spectrophotometry (AAS)
analysis revealed significant cadmium load in duodenum,which was
associated with changes of both intestinal bacterial load and composition
(Denaturing Gradient Gel Electrophoresis/DGGE).Shortening of villi,
damage to epithelium, increases in goblet-like vacuoles and mononuclear
cell infiltration in lamina propria were histologically evident at both
cadmium doses, with massive necrosis at higher Cd dose (judging by High
Mobility Group Box-1/HMGB-1 Western blot analysis).Increased levels of
proinflammatory cytokines (IL-1β, IFN-γ, IL-17) were observed at both Cd
doses (TNFα at higher dose solely). Cadmium administration affected
draining lymph nodes as well, judging by signs of stress response (drop of
cellular reduced glutathione/GSH at higher dose, rise of
metallothionein/MT mRNA at both doses).Increased cellularity of mLN
was observed at higher Cd dose, but with no changes in proliferative
responses. Intake of both Cd doses resulted in higher (compared to controls)
levels of IFN-γ and IL-17 mRNA as well as increased mLN cell
responsiveness to ConA stimulation.Significant increases in numbers of
CD68+ cells and stimulation of innate immune activities (numbers of
dihydrorhodamine/DHR+ cells and intracellular myeloperoxidase/MPO+
cells, LPS-stimulated nitric oxide/NO production and ex vivo IL-1β
expression) were observed at higher dose of cadmium.Proinflammatory
effects of cadmium might have resulted from changes in gut microbiota and
tissue damage.Interactions of this metal with intestinal immune system
should be taken into account when assessing dietary cadmium as health risk
factor.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia",
title = "Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4811"
}

Lukić, M., Jonjic, S., Nikolich-Zugich, J., Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Mileusnić, D., Grigorov, I., Petrović, A., Zolotarevski, L., Nikolic, M.,& Kataranovski, M.. (2015). Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia
Belgrade: Immunological Society of Serbia., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_4811

Lukić M, Jonjic S, Nikolich-Zugich J, Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Mileusnić D, Grigorov I, Petrović A, Zolotarevski L, Nikolic M, Kataranovski M. Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats. in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia. 2015;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_4811 .

Lukić, Miodrag, Jonjic, Stipan, Nikolich-Zugich, Janko, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Mileusnić, Dina, Grigorov, Ilijana, Petrović, Anja, Zolotarevski, Lidija, Nikolic, Milica, Kataranovski, Milena, "Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats" in 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging, May 24-27, 2015, Arandjelovac, Serbia (2015):48,
https://hdl.handle.net/21.15107/rcub_ibiss_4811 .