TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrif.bg.ac.rs/uploads/files/strane/Fakultet/Izdavacka_delatnost/Zbornici_radova/XI%20Simpozijum%20sa%20medjunarodnim%20ucescem%20-%20Inovacije%20u%20ratarskoj%20i%20povrtarskoj%20proizvodnji.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6487
AB  - Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility.
AB  - Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
T1  - Potato minituber production by aeroponics: effects of plant origin and genotype
T1  - Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6487
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility., Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia",
title = "Potato minituber production by aeroponics: effects of plant origin and genotype, Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6487"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia. 2023;:31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Potato minituber production by aeroponics: effects of plant origin and genotype" in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia (2023):31-32,
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrores.agro.unibl.org/wp-content/uploads/2023/05/Book-of-Proceedings-AgroReS-2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6488
AB  - Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.
PB  - Banja Luka: University of Banja Luka, Faculty of Agriculture
C3  - Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
T1  - Effects of cultivar and plant origin on the aeroponic production of potato minitubers
DO  - 10.7251/ZARS2301009B
SP  - 9
EP  - 18
ER  - 

@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.",
publisher = "Banja Luka: University of Banja Luka, Faculty of Agriculture",
journal = "Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina",
title = "Effects of cultivar and plant origin on the aeroponic production of potato minitubers",
doi = "10.7251/ZARS2301009B",
pages = "9-18"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
Banja Luka: University of Banja Luka, Faculty of Agriculture., 9-18.
https://doi.org/10.7251/ZARS2301009B

Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina. 2023;:9-18.
doi:10.7251/ZARS2301009B .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Effects of cultivar and plant origin on the aeroponic production of potato minitubers" in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina (2023):9-18,
https://doi.org/10.7251/ZARS2301009B . .

TY  - JOUR
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5122
AB  - Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Potato aeroponics: effects of cultivar and plant origin on minituber production
IS  - 10
VL  - 8
DO  - 10.3390/horticulturae8100915
SP  - 915
ER  - 

@article{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2022",
abstract = "Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Potato aeroponics: effects of cultivar and plant origin on minituber production",
number = "10",
volume = "8",
doi = "10.3390/horticulturae8100915",
pages = "915"
}

Broćić, Z., Oljača, J., Pantelić, D., Rudić, J.,& Momčilović, I.. (2022). Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae
Basel: MDPI., 8(10), 915.
https://doi.org/10.3390/horticulturae8100915

Broćić Z, Oljača J, Pantelić D, Rudić J, Momčilović I. Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae. 2022;8(10):915.
doi:10.3390/horticulturae8100915 .

Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Potato aeroponics: effects of cultivar and plant origin on minituber production" in Horticulturae, 8, no. 10 (2022):915,
https://doi.org/10.3390/horticulturae8100915 . .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/5/372
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4979
AB  - Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants
IS  - 5
VL  - 8
DO  - 10.3390/horticulturae8050372
SP  - 372
ER  - 

@article{
author = "Rudić, Jelena and Pantelić, Danijel and Oljača, Jasmina and Momčilović, Ivana",
year = "2022",
abstract = "Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants",
number = "5",
volume = "8",
doi = "10.3390/horticulturae8050372",
pages = "372"
}

Rudić, J., Pantelić, D., Oljača, J.,& Momčilović, I.. (2022). Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae
Basel: MDPI., 8(5), 372.
https://doi.org/10.3390/horticulturae8050372

Rudić J, Pantelić D, Oljača J, Momčilović I. Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae. 2022;8(5):372.
doi:10.3390/horticulturae8050372 .

Rudić, Jelena, Pantelić, Danijel, Oljača, Jasmina, Momčilović, Ivana, "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants" in Horticulturae, 8, no. 5 (2022):372,
https://doi.org/10.3390/horticulturae8050372 . .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Momčilović, Ivana
AU  - Simonović, Ana
AU  - Pantelić, Danijel
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/3/488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4875
AB  - Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.
T2  - Antioxidants
T1  - In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression
IS  - 3
VL  - 11
DO  - 10.3390/antiox11030488
SP  - 488
ER  - 

@article{
author = "Rudić, Jelena and Dragićević, Milan and Momčilović, Ivana and Simonović, Ana and Pantelić, Danijel",
year = "2022",
abstract = "Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.",
journal = "Antioxidants",
title = "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression",
number = "3",
volume = "11",
doi = "10.3390/antiox11030488",
pages = "488"
}

Rudić, J., Dragićević, M., Momčilović, I., Simonović, A.,& Pantelić, D.. (2022). In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants, 11(3), 488.
https://doi.org/10.3390/antiox11030488

Rudić J, Dragićević M, Momčilović I, Simonović A, Pantelić D. In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants. 2022;11(3):488.
doi:10.3390/antiox11030488 .

Rudić, Jelena, Dragićević, Milan, Momčilović, Ivana, Simonović, Ana, Pantelić, Danijel, "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression" in Antioxidants, 11, no. 3 (2022):488,
https://doi.org/10.3390/antiox11030488 . .

TY  - CONF
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5124
AB  - Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5124
ER  - 

@conference{
editor = "Milutinović, Milica",
author = "Rudić, Jelena and Pantelić, Danijel and Momčilović, Ivana",
year = "2022",
abstract = "Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5124"
}

Milutinović, M., Rudić, J., Pantelić, D.,& Momčilović, I.. (2022). Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5124

Milutinović M, Rudić J, Pantelić D, Momčilović I. Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5124 .

Milutinović, Milica, Rudić, Jelena, Pantelić, Danijel, Momčilović, Ivana, "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L." in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5124 .

TY  - CONF
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5123
AB  - Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5123
ER  - 

@conference{
author = "Rudić, Jelena and Pantelić, Danijel and Momčilović, Ivana",
year = "2022",
abstract = "Potato, Solanum tuberosum L., is the leading vegetable crop in the world and is highly vulnerable to elevated ambient temperatures. Since high-temperature stress causes the accumulation of reactive oxygen species (ROS) in plant cells, investigations regarding major enzymatic components of the potato antioxidative system are of importance. Superoxide dismutases (SODs) are metalloenzymes that play an important role as primary ROS scavengers. Previously, we conducted a detailed in silico analysis and characterization of the potato SOD gene family. In the present study, we analyzed the expression of potato SOD-encoding genes, three StCu/ZnSODs, one StMnSOD and four StFeSODs, in different organs of potato cv. Désirée plants at optimal growth conditions (20/17 °C, 70% humidity) and during mild heat stress (30/20 °C, 70% humidity) on the 1st, 7th and 21st day of treatment. Our results revealed that cytosolic StCu/ZnSOD1, chloroplast StCu/ZnSOD2 and StFeSOD3, and mitochondrial StMnSOD were characterized by a high level of expression in potato organs, especially leaves and stolons. Conversely, chloroplast StFeSOD1 and StFeSOD4 expression levels were very low in all investigated organs. Under mild heat stress, the significant increase in the abundance of most StSODs transcripts was mainly observed in leaves and stolons after 7 days of the treatment. Interestingly, transcript levels of all eight potato StSODs were the lowest in developing tubers compared to other organs, regardless of temperature treatment. Our findings provide insight into the modulation of organ-specific expression of eight potato SODs by elevated temperature and may contribute to a better understanding of potato antioxidant response to mild heat stress.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5123"
}

Rudić, J., Pantelić, D.,& Momčilović, I.. (2022). Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5123

Rudić J, Pantelić D, Momčilović I. Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L.. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5123 .

Rudić, Jelena, Pantelić, Danijel, Momčilović, Ivana, "Effects of elevated temperature on the organ-specific expression of superoxide dismutase gene family in potato, Solanum tuberosum L." in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5123 .

TY  - CHAP
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
AU  - Poštić, Dobrivoj
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4471
AB  - Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.
PB  - New York : Nova Science Publishers Inc.
T2  - The Potato Crop: Management, Production, and Food Security
T1  - Production of High-Quality Seed Potato by Aeroponics
DO  - 10.52305/RHLO1469
SP  - 25
EP  - 59
ER  - 

@inbook{
editor = "Villa, Pedro",
author = "Broćić, Zoran and Momčilović, Ivana and Poštić, Dobrivoj and Oljača, Jasmina and Veljković, Biljana",
year = "2021",
abstract = "Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.",
publisher = "New York : Nova Science Publishers Inc.",
journal = "The Potato Crop: Management, Production, and Food Security",
booktitle = "Production of High-Quality Seed Potato by Aeroponics",
doi = "10.52305/RHLO1469",
pages = "25-59"
}

Villa, P., Broćić, Z., Momčilović, I., Poštić, D., Oljača, J.,& Veljković, B.. (2021). Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security
New York : Nova Science Publishers Inc.., 25-59.
https://doi.org/10.52305/RHLO1469

Villa P, Broćić Z, Momčilović I, Poštić D, Oljača J, Veljković B. Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security. 2021;:25-59.
doi:10.52305/RHLO1469 .

Villa, Pedro, Broćić, Zoran, Momčilović, Ivana, Poštić, Dobrivoj, Oljača, Jasmina, Veljković, Biljana, "Production of High-Quality Seed Potato by Aeroponics" in The Potato Crop: Management, Production, and Food Security (2021):25-59,
https://doi.org/10.52305/RHLO1469 . .

TY  - CONF
AU  - Momčilović, Ivana
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4673
AB  - Potato, Solanum tuberosum L., is the most important non-grain food crop in the world and the main vegetable crop in Serbia. This species produces more food per unit of 
production area than any cereal crop and significantly contributes to global food security.
However, potato is highly susceptible to two major abiotic stresses: heat stress and drought.
These stresses may significantly affect the growth of potato plants at each of the developmental stages they emerge, ultimately causing a reduction in plant productivity due to the
smaller number of the formed tubers and lower tuber mass. High temperatures and/or water deficit also cause various tuber disorders, such as shape deformation, growth cracks,
secondary tuberization and skin netting or russeting, decreasing the proportion of marketable tubers. One of the disorders associated with heat stress accompanied by drought is
“sugar ends”, which adversely affects the processing and nutritional qualities of the tubers.
Due to global climate change, and the continuous rise in the Earth’s surface temperatures,
the detrimental effects of heat stress and drought on potato are intensifying. The global average surface temperature had already increased by 1.2 °C above pre-industrial levels, and
it will likely reach 1.5 °C by the year 2030. The impact of climate change will differ between
regions. At lower latitudes, especially in seasonally dry and tropical regions, potato productivity is projected to decrease even for small local temperature increases. By contrast, the
impact of climate change on potato yields might be beneficial for growing potatoes at higher
altitudes and higher latitudes. Nevertheless, a decline in global potato yields between 18-
32% by the year 2050 is expected, according to simulation-based analysis. World population
growth requires a significant increase in potato production, and solutions for mitigating the
damage of major abiotic stresses on potato productivity and quality are needed. The adverse
effects of heat stress and drought on potato can be alleviated to a certain extend by a shift
in planting time and application of cultivation technologies such as irrigation, mulching,
plant shading, intercropping, and plant growth regulator treatments. However, a principal
approach to mitigate damage is the development of heat- and drought-tolerant varieties. In
the past decades, significant progress was made towards understanding the mechanisms associated with potato heat- and drought-susceptibility, while the breeders’ efforts intensified
to select new, tolerant potato genotypes.
PB  - Belgrade: Serbian Nutrition Society
C3  - 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
T1  - Impact of climate changes on potato production and mitigation strategies
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4673
ER  - 

@conference{
author = "Momčilović, Ivana and Pantelić, Danijel and Rudić, Jelena",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the most important non-grain food crop in the world and the main vegetable crop in Serbia. This species produces more food per unit of 
production area than any cereal crop and significantly contributes to global food security.
However, potato is highly susceptible to two major abiotic stresses: heat stress and drought.
These stresses may significantly affect the growth of potato plants at each of the developmental stages they emerge, ultimately causing a reduction in plant productivity due to the
smaller number of the formed tubers and lower tuber mass. High temperatures and/or water deficit also cause various tuber disorders, such as shape deformation, growth cracks,
secondary tuberization and skin netting or russeting, decreasing the proportion of marketable tubers. One of the disorders associated with heat stress accompanied by drought is
“sugar ends”, which adversely affects the processing and nutritional qualities of the tubers.
Due to global climate change, and the continuous rise in the Earth’s surface temperatures,
the detrimental effects of heat stress and drought on potato are intensifying. The global average surface temperature had already increased by 1.2 °C above pre-industrial levels, and
it will likely reach 1.5 °C by the year 2030. The impact of climate change will differ between
regions. At lower latitudes, especially in seasonally dry and tropical regions, potato productivity is projected to decrease even for small local temperature increases. By contrast, the
impact of climate change on potato yields might be beneficial for growing potatoes at higher
altitudes and higher latitudes. Nevertheless, a decline in global potato yields between 18-
32% by the year 2050 is expected, according to simulation-based analysis. World population
growth requires a significant increase in potato production, and solutions for mitigating the
damage of major abiotic stresses on potato productivity and quality are needed. The adverse
effects of heat stress and drought on potato can be alleviated to a certain extend by a shift
in planting time and application of cultivation technologies such as irrigation, mulching,
plant shading, intercropping, and plant growth regulator treatments. However, a principal
approach to mitigate damage is the development of heat- and drought-tolerant varieties. In
the past decades, significant progress was made towards understanding the mechanisms associated with potato heat- and drought-susceptibility, while the breeders’ efforts intensified
to select new, tolerant potato genotypes.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia",
title = "Impact of climate changes on potato production and mitigation strategies",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4673"
}

Momčilović, I., Pantelić, D.,& Rudić, J.. (2021). Impact of climate changes on potato production and mitigation strategies. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 66-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4673

Momčilović I, Pantelić D, Rudić J. Impact of climate changes on potato production and mitigation strategies. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia. 2021;:66-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4673 .

Momčilović, Ivana, Pantelić, Danijel, Rudić, Jelena, "Impact of climate changes on potato production and mitigation strategies" in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia (2021):66-66,
https://hdl.handle.net/21.15107/rcub_ibiss_4673 .

TY  - CHAP
AU  - Momčilović, Ivana
AU  - Fu, Jianming
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Broćić, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4470
AB  - Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, which produces more food per unit of production area than any cereal crop and significantly contributes to global food security. Potato domestication originated in a cool climate region of the Andes and, due to its narrow genetic basis of heat tolerance traits, this vegetable species is highly susceptible to heat stress. High-temperature stress delays sprout emergence, reduces leaf size, total leaf area, the number of stolons, the number and mass of tubers, and causes tuber disorders, consequently reducing the total and marketable yield of potato. Heat stress also negatively affects the nutritive and processing qualities of tubers. Due to global climate change, and the continuous rise in the Earth's surface temperatures, the detrimental effects of heat stress on potato crop are intensifying. At the same time, world population growth requires a significant increase in potato production. Thus, solutions for mitigating the damage of high-temperature stress on potato productivity are essential. In the past decades, various researches on the effects of high temperatures on potato plants have been conducted, and significant progress towards understanding the mechanisms underlying potato heat stress responses at the whole plant and molecular levels has been made. This chapter summarizes the current knowledge of the effects of heat stress on potato growth, development, productivity and nutritive qualities, and mechanisms associated with potato heat susceptibility. Future research directions and the development of potato varieties with improved heat tolerance are also discussed.
PB  - New York : Nova Science Publishers Inc.
T2  - The Potato Crop: Management, Production, and Food Security
T1  - Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement
DO  - 10.52305/RHLO1469
SP  - 91
EP  - 122
ER  - 

@inbook{
editor = "Villa, Pedro",
author = "Momčilović, Ivana and Fu, Jianming and Pantelić, Danijel and Rudić, Jelena and Broćić, Zoran",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, which produces more food per unit of production area than any cereal crop and significantly contributes to global food security. Potato domestication originated in a cool climate region of the Andes and, due to its narrow genetic basis of heat tolerance traits, this vegetable species is highly susceptible to heat stress. High-temperature stress delays sprout emergence, reduces leaf size, total leaf area, the number of stolons, the number and mass of tubers, and causes tuber disorders, consequently reducing the total and marketable yield of potato. Heat stress also negatively affects the nutritive and processing qualities of tubers. Due to global climate change, and the continuous rise in the Earth's surface temperatures, the detrimental effects of heat stress on potato crop are intensifying. At the same time, world population growth requires a significant increase in potato production. Thus, solutions for mitigating the damage of high-temperature stress on potato productivity are essential. In the past decades, various researches on the effects of high temperatures on potato plants have been conducted, and significant progress towards understanding the mechanisms underlying potato heat stress responses at the whole plant and molecular levels has been made. This chapter summarizes the current knowledge of the effects of heat stress on potato growth, development, productivity and nutritive qualities, and mechanisms associated with potato heat susceptibility. Future research directions and the development of potato varieties with improved heat tolerance are also discussed.",
publisher = "New York : Nova Science Publishers Inc.",
journal = "The Potato Crop: Management, Production, and Food Security",
booktitle = "Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement",
doi = "10.52305/RHLO1469",
pages = "91-122"
}

Villa, P., Momčilović, I., Fu, J., Pantelić, D., Rudić, J.,& Broćić, Z.. (2021). Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement. in The Potato Crop: Management, Production, and Food Security
New York : Nova Science Publishers Inc.., 91-122.
https://doi.org/10.52305/RHLO1469

Villa P, Momčilović I, Fu J, Pantelić D, Rudić J, Broćić Z. Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement. in The Potato Crop: Management, Production, and Food Security. 2021;:91-122.
doi:10.52305/RHLO1469 .

Villa, Pedro, Momčilović, Ivana, Fu, Jianming, Pantelić, Danijel, Rudić, Jelena, Broćić, Zoran, "Impact of Heat Stress on Potato: Plant Responses and Approaches to Tolerance Improvement" in The Potato Crop: Management, Production, and Food Security (2021):91-122,
https://doi.org/10.52305/RHLO1469 . .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2021
UR  - https://www.vibconferences.be/events/plant-science-for-climate-emergency
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4340
AB  - Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.
PB  - VIB Conferences Series
C3  - Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
T1  - The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression
SP  - 97 / P38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4340
ER  - 

@conference{
author = "Pantelić, Danijel and Rudić, Jelena and Dragićević, Milan and Simonović, Ana and Momčilović, Ivana",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.",
publisher = "VIB Conferences Series",
journal = "Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.",
title = "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression",
pages = "97 / P38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4340"
}

Pantelić, D., Rudić, J., Dragićević, M., Simonović, A.,& Momčilović, I.. (2021). The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
VIB Conferences Series., 97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340

Pantelić D, Rudić J, Dragićević M, Simonović A, Momčilović I. The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.. 2021;:97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

Pantelić, Danijel, Rudić, Jelena, Dragićević, Milan, Simonović, Ana, Momčilović, Ivana, "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression" in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97. (2021):97 / P38,
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Pantelić, Danijel
AU  - Milošević, Snežana
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1589
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4009
AB  - Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
PB  - MDPI AG
T2  - Plants
T1  - Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana
IS  - 11
VL  - 9
DO  - 10.3390/plants9111589
SP  - 1589
ER  - 

@article{
author = "Antonić Reljin, Dragana and Subotić, Angelina and Dragićević, Milan and Pantelić, Danijel and Milošević, Snežana and Simonović, Ana and Momčilović, Ivana",
year = "2020",
abstract = "Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana",
number = "11",
volume = "9",
doi = "10.3390/plants9111589",
pages = "1589"
}

Antonić Reljin, D., Subotić, A., Dragićević, M., Pantelić, D., Milošević, S., Simonović, A.,& Momčilović, I.. (2020). Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants
MDPI AG., 9(11), 1589.
https://doi.org/10.3390/plants9111589

Antonić Reljin D, Subotić A, Dragićević M, Pantelić D, Milošević S, Simonović A, Momčilović I. Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants. 2020;9(11):1589.
doi:10.3390/plants9111589 .

Antonić Reljin, Dragana, Subotić, Angelina, Dragićević, Milan, Pantelić, Danijel, Milošević, Snežana, Simonović, Ana, Momčilović, Ivana, "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana" in Plants, 9, no. 11 (2020):1589,
https://doi.org/10.3390/plants9111589 . .

TY  - CONF
AU  - Momčilović, Ivana
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4362
AB  - Protein synthesis elongation factors EF-Tu and eEF1A (EFs) are highly conserved and abundant GTPases in living cells, with a major role in transporting the aminoacyl-tRNA complex to the ribosome during translation. EFs are also considered multifunctional proteins with numerous noncanonical activities. EFs possess chaperone activity in preventing protein aggregation, interact with misfolded newly synthesized polypeptides and possibly direct them to the proteasome, and participate in viral replication. eEF1A is also implicated in the organization of cytoskeleton and apoptosis, while EF-Tu of bacterial pathogens elicits plant innate immunity. There is growing evidence that up-regulation of EFs genes by abiotic stresses may play an important role in stress responses in plants. The heat stress-induced accumulation of EFs has been detected in a number of plant species, and it correlates with the heat tolerant phenotype in maize, wheat, and potato. Accumulation of EFs may enhance protein synthesis and the capability of plant cells to sustain heat stress. Besides, the proposed EFs role in the quality control of newly synthesized proteins and chaperone activity in preventing protein thermal aggregation and inactivation might be important in alleviating negative effects of high temperature. Taken together, these multifunctional proteins may represent coordinators of the cellular processes in stressed plants, for instance, protein synthesis with recognition and repair/degradation of misfolded proteins.
PB  - Belgrade: Faculty of Chemistry
C3  - Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings
T1  - Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4362
ER  - 

@conference{
author = "Momčilović, Ivana",
year = "2019",
abstract = "Protein synthesis elongation factors EF-Tu and eEF1A (EFs) are highly conserved and abundant GTPases in living cells, with a major role in transporting the aminoacyl-tRNA complex to the ribosome during translation. EFs are also considered multifunctional proteins with numerous noncanonical activities. EFs possess chaperone activity in preventing protein aggregation, interact with misfolded newly synthesized polypeptides and possibly direct them to the proteasome, and participate in viral replication. eEF1A is also implicated in the organization of cytoskeleton and apoptosis, while EF-Tu of bacterial pathogens elicits plant innate immunity. There is growing evidence that up-regulation of EFs genes by abiotic stresses may play an important role in stress responses in plants. The heat stress-induced accumulation of EFs has been detected in a number of plant species, and it correlates with the heat tolerant phenotype in maize, wheat, and potato. Accumulation of EFs may enhance protein synthesis and the capability of plant cells to sustain heat stress. Besides, the proposed EFs role in the quality control of newly synthesized proteins and chaperone activity in preventing protein thermal aggregation and inactivation might be important in alleviating negative effects of high temperature. Taken together, these multifunctional proteins may represent coordinators of the cellular processes in stressed plants, for instance, protein synthesis with recognition and repair/degradation of misfolded proteins.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings",
title = "Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4362"
}

Momčilović, I.. (2019). Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance. in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings
Belgrade: Faculty of Chemistry., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_4362

Momčilović I. Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance. in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings. 2019;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_4362 .

Momčilović, Ivana, "Elongation factors Tu and 1A: multifunctional proteins involved in plant heat tolerance" in Serbian Biochemical Society Ninth Conference with international participation “Diversity in Biochemistry”, Proceedings (2019):41,
https://hdl.handle.net/21.15107/rcub_ibiss_4362 .

TY  - JOUR
AU  - Momčilović, Ivana
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3771
AB  - Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, and a major vegetable crop in Serbia. It is generally considered a cool-season crop and highly susceptible to high temperatures. Elevated temperatures in the environment affect potato plants' growth and development by slowing the sprout emergence, reducing the number of stolons, impeding tuber initiation, reducing tuber bulking and interfering the onset of tuber dormancy. Tuberization is optimal at average daily temperatures in the 15-20 °C range and above this range declines, although moderately elevated temperatures of 20-25 °C may enhance potato foliage growth and the net photosynthesis. Besides reducing the number and mass of tubers, high-temperature stress affects the total and marketable yield of potato by causing tuber disorders and altering tuber processing and nutritive quality. The problem of potato heat-susceptibility is gaining more interest in the last decades due to occurring global climate change. The breeders' efforts have been intensified for selection of new, tolerant potato genotypes, as well as genomics', proteomics', and metabolomics' investigations of potato heat response.
PB  - Beograd: Društvo za ishranu Srbije
T2  - Hrana i ishrana
T1  - Effects of heat stress on potato productivity and nutritive quality
IS  - 2
VL  - 60
DO  - 10.5937/hraIsh1902043M
SP  - 43
EP  - 48
ER  - 

@article{
author = "Momčilović, Ivana",
year = "2019",
abstract = "Potato, Solanum tuberosum L., is the most important non-grain food crop in the world, and a major vegetable crop in Serbia. It is generally considered a cool-season crop and highly susceptible to high temperatures. Elevated temperatures in the environment affect potato plants' growth and development by slowing the sprout emergence, reducing the number of stolons, impeding tuber initiation, reducing tuber bulking and interfering the onset of tuber dormancy. Tuberization is optimal at average daily temperatures in the 15-20 °C range and above this range declines, although moderately elevated temperatures of 20-25 °C may enhance potato foliage growth and the net photosynthesis. Besides reducing the number and mass of tubers, high-temperature stress affects the total and marketable yield of potato by causing tuber disorders and altering tuber processing and nutritive quality. The problem of potato heat-susceptibility is gaining more interest in the last decades due to occurring global climate change. The breeders' efforts have been intensified for selection of new, tolerant potato genotypes, as well as genomics', proteomics', and metabolomics' investigations of potato heat response.",
publisher = "Beograd: Društvo za ishranu Srbije",
journal = "Hrana i ishrana",
title = "Effects of heat stress on potato productivity and nutritive quality",
number = "2",
volume = "60",
doi = "10.5937/hraIsh1902043M",
pages = "43-48"
}

Momčilović, I.. (2019). Effects of heat stress on potato productivity and nutritive quality. in Hrana i ishrana
Beograd: Društvo za ishranu Srbije., 60(2), 43-48.
https://doi.org/10.5937/hraIsh1902043M

Momčilović I. Effects of heat stress on potato productivity and nutritive quality. in Hrana i ishrana. 2019;60(2):43-48.
doi:10.5937/hraIsh1902043M .

Momčilović, Ivana, "Effects of heat stress on potato productivity and nutritive quality" in Hrana i ishrana, 60, no. 2 (2019):43-48,
https://doi.org/10.5937/hraIsh1902043M . .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Oljača, Jasmina
AU  - Simonović, Ana
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4348
AB  - Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance
SP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4348
ER  - 

@conference{
editor = "Kovačević, Dušan",
author = "Pantelić, Danijel and Rudić, Jelena and Oljača, Jasmina and Simonović, Ana and Broćić, Zoran and Momčilović, Ivana",
year = "2018",
abstract = "Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4348"
}

Kovačević, D., Pantelić, D., Rudić, J., Oljača, J., Simonović, A., Broćić, Z.,& Momčilović, I.. (2018). Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348

Kovačević D, Pantelić D, Rudić J, Oljača J, Simonović A, Broćić Z, Momčilović I. Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2018;:335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

Kovačević, Dušan, Pantelić, Danijel, Rudić, Jelena, Oljača, Jasmina, Simonović, Ana, Broćić, Zoran, Momčilović, Ivana, "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance" in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina (2018):335,
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

TY  - CONF
AU  - Oljača, Jasmina
AU  - Broćič, Zoran
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4349
AB  - Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - IX International Scientific Agriculture Symposium “Agrosym 2018”
T1  - Prospective protein markers for selection of heat tolerant potato cultivars
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4349
ER  - 

@conference{
author = "Oljača, Jasmina and Broćič, Zoran and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2018",
abstract = "Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "IX International Scientific Agriculture Symposium “Agrosym 2018”",
title = "Prospective protein markers for selection of heat tolerant potato cultivars",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4349"
}

Oljača, J., Broćič, Z., Pantelić, D., Rudić, J.,& Momčilović, I.. (2018). Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349

Oljača J, Broćič Z, Pantelić D, Rudić J, Momčilović I. Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”. 2018;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

Oljača, Jasmina, Broćič, Zoran, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Prospective protein markers for selection of heat tolerant potato cultivars" in IX International Scientific Agriculture Symposium “Agrosym 2018” (2018):340,
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

TY  - JOUR
AU  - Pantelić, Danijel
AU  - Dragićević, Ivana Č.
AU  - Rudić, Jelena
AU  - Fu, Jianming
AU  - Momčilović, Ivana
PY  - 2018
UR  - http://link.springer.com/10.1007/s13580-018-0043-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3089
AB  - Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.
T2  - Horticulture, Environment, and Biotechnology
T1  - Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato
IS  - 3
VL  - 59
DO  - 10.1007/s13580-018-0043-x
SP  - 315
EP  - 324
ER  - 

@article{
author = "Pantelić, Danijel and Dragićević, Ivana Č. and Rudić, Jelena and Fu, Jianming and Momčilović, Ivana",
year = "2018",
abstract = "Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.",
journal = "Horticulture, Environment, and Biotechnology",
title = "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato",
number = "3",
volume = "59",
doi = "10.1007/s13580-018-0043-x",
pages = "315-324"
}

Pantelić, D., Dragićević, I. Č., Rudić, J., Fu, J.,& Momčilović, I.. (2018). Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology, 59(3), 315-324.
https://doi.org/10.1007/s13580-018-0043-x

Pantelić D, Dragićević IČ, Rudić J, Fu J, Momčilović I. Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. in Horticulture, Environment, and Biotechnology. 2018;59(3):315-324.
doi:10.1007/s13580-018-0043-x .

Pantelić, Danijel, Dragićević, Ivana Č., Rudić, Jelena, Fu, Jianming, Momčilović, Ivana, "Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato" in Horticulture, Environment, and Biotechnology, 59, no. 3 (2018):315-324,
https://doi.org/10.1007/s13580-018-0043-x . .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Paunović, Danijela
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4338
AB  - Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Expression of dehydrins in Impatiens walleriana exposed to drought
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4338
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Dragićević, Milan and Simonović, Ana and Paunović, Danijela and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Expression of dehydrins in Impatiens walleriana exposed to drought",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4338"
}

Antonić, D., Milošević, S., Dragićević, M., Simonović, A., Paunović, D., Momčilović, I.,& Subotić, A.. (2018). Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338

Antonić D, Milošević S, Dragićević M, Simonović A, Paunović D, Momčilović I, Subotić A. Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

Antonić, Dragana, Milošević, Snežana, Dragićević, Milan, Simonović, Ana, Paunović, Danijela, Momčilović, Ivana, Subotić, Angelina, "Expression of dehydrins in Impatiens walleriana exposed to drought" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):74,
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CHAP
AU  - Momčilović, Ivana
AU  - Pantelić, Danijel
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3029
AB  - Abiotic stresses affect plant growth and development, and significantly reduce crop productivity by causing the alteration in cell biochemistry. Common for all abiotic stresses is that they cause an imbalance between production and scavenging of reactive oxygen species (ROS) in cells, resulting in elevation of ROS concentrations to cytotoxic level. Superoxide dismutases (SODs) are enzymes that play an important role of primary ROS scavengers by catalyzing the conversion of superoxide anion radical to hydrogen peroxide and oxygen. Most plant species contain numerous SOD isoforms which are classified according to the active site metal into three major types: FeSOD, MnSOD and Cu/ZnSOD. All plant SODs are nuclear-encoded, synthesized on cytosolic ribosomes and transported into various cell compartments, such as cytosol, plastids, mitochondria, peroxisomes and cell wall. Expression of SODs genes might be modulated by developmental and environmental cues, including nutrition and abiotic stresses. So far, enhanced expression and/or activity of SODs have been reported in response to various abiotic stressors, such as water deficit, high salinity, heavy metals, hypoxia, high and low temperatures. This chapter summarizes the current knowledge of plant SODs, their abiotic-stress modulated expression and activity, and analyses results on genetic engineering of plant SODs. The significance of superoxide dismutases in the crop improvement for stress tolerance is also discussed.
PB  - Nova Science Publishers, Inc.
T2  - Superoxide Dismutase: Structure, Synthesis and Applications
T1  - Plant superoxide dismutases: Important players in abiotic stress tolerance
SP  - 45
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3029
ER  - 

@inbook{
author = "Momčilović, Ivana and Pantelić, Danijel",
year = "2018",
abstract = "Abiotic stresses affect plant growth and development, and significantly reduce crop productivity by causing the alteration in cell biochemistry. Common for all abiotic stresses is that they cause an imbalance between production and scavenging of reactive oxygen species (ROS) in cells, resulting in elevation of ROS concentrations to cytotoxic level. Superoxide dismutases (SODs) are enzymes that play an important role of primary ROS scavengers by catalyzing the conversion of superoxide anion radical to hydrogen peroxide and oxygen. Most plant species contain numerous SOD isoforms which are classified according to the active site metal into three major types: FeSOD, MnSOD and Cu/ZnSOD. All plant SODs are nuclear-encoded, synthesized on cytosolic ribosomes and transported into various cell compartments, such as cytosol, plastids, mitochondria, peroxisomes and cell wall. Expression of SODs genes might be modulated by developmental and environmental cues, including nutrition and abiotic stresses. So far, enhanced expression and/or activity of SODs have been reported in response to various abiotic stressors, such as water deficit, high salinity, heavy metals, hypoxia, high and low temperatures. This chapter summarizes the current knowledge of plant SODs, their abiotic-stress modulated expression and activity, and analyses results on genetic engineering of plant SODs. The significance of superoxide dismutases in the crop improvement for stress tolerance is also discussed.",
publisher = "Nova Science Publishers, Inc.",
journal = "Superoxide Dismutase: Structure, Synthesis and Applications",
booktitle = "Plant superoxide dismutases: Important players in abiotic stress tolerance",
pages = "45-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3029"
}

Momčilović, I.,& Pantelić, D.. (2018). Plant superoxide dismutases: Important players in abiotic stress tolerance. in Superoxide Dismutase: Structure, Synthesis and Applications
Nova Science Publishers, Inc.., 45-100.
https://hdl.handle.net/21.15107/rcub_ibiss_3029

Momčilović I, Pantelić D. Plant superoxide dismutases: Important players in abiotic stress tolerance. in Superoxide Dismutase: Structure, Synthesis and Applications. 2018;:45-100.
https://hdl.handle.net/21.15107/rcub_ibiss_3029 .

Momčilović, Ivana, Pantelić, Danijel, "Plant superoxide dismutases: Important players in abiotic stress tolerance" in Superoxide Dismutase: Structure, Synthesis and Applications (2018):45-100,
https://hdl.handle.net/21.15107/rcub_ibiss_3029 .

TY  - JOUR
AU  - Momčilović, Ivana
AU  - Pantelic, Danijel
AU  - Hfidan, Masoud
AU  - Savic, Jelena
AU  - Vinterhalter, Dragan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2180
AB  - Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.
T2  - Acta Physiologiae Plantarum
T1  - Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.
IS  - 8
VL  - 36
DO  - 10.1007/s11738-014-1583-z
SP  - 2059
EP  - 2066
ER  - 

@article{
author = "Momčilović, Ivana and Pantelic, Danijel and Hfidan, Masoud and Savic, Jelena and Vinterhalter, Dragan",
year = "2014",
abstract = "Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.",
journal = "Acta Physiologiae Plantarum",
title = "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.",
number = "8",
volume = "36",
doi = "10.1007/s11738-014-1583-z",
pages = "2059-2066"
}

Momčilović, I., Pantelic, D., Hfidan, M., Savic, J.,& Vinterhalter, D.. (2014). Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum, 36(8), 2059-2066.
https://doi.org/10.1007/s11738-014-1583-z

Momčilović I, Pantelic D, Hfidan M, Savic J, Vinterhalter D. Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum. 2014;36(8):2059-2066.
doi:10.1007/s11738-014-1583-z .

Momčilović, Ivana, Pantelic, Danijel, Hfidan, Masoud, Savic, Jelena, Vinterhalter, Dragan, "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L." in Acta Physiologiae Plantarum, 36, no. 8 (2014):2059-2066,
https://doi.org/10.1007/s11738-014-1583-z . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Zdravković-Korać, Snežana
AU  - Momčilović, Ivana
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2181
AB  - Proteinase inhibitors oryzacystatins I and II (OCI and OCII) have shown
   potential in controlling pests that utilize cysteine proteinases for
   protein digestion. In order to achieve an additive effect against
   Colorado potato beetle (CPB, Leptinotarsa decemlineata Say) digestive
   proteinases, we combined two individual OC genes in potato (Solanum
   tuberosum L.) cultivars Desiree, Dragaevka and Jelica. The OC genes were
   stacked into the potato genome by simultaneous co-transformation with
   both OCI and OCII genes or by sequential re-transformation of an
   OCI-expressing transgenic line with an OCII gene. Low constitutive and
   high wound-induced transcript levels of both OCI and OCII genes,
   directed by the inducible pin2 promoter, were detected in all doubly
   transformed lines from all three cultivars. Both recombinant OCs,
   detected by immunoblot, were in an active conformation as shown by their
   strong papain inhibitory activity. Although no significant differences
   in CPB larval mortality were observed when larvae fed on OCI/OCII
   transformed or control potato foliage, expression of both OCI and OCII
   in potato plants caused a reduction in CPB adult body weight and in
   weight of foliage consumed, indicating an additive effect. Overall,
   these results show that pyramiding of the OCI and OCII genes could be an
   effective strategy for breeding cultivars exhibiting elevated levels of
   resistance to CPB.
T2  - Euphytica
T1  - Pyramiding rice cystatin OCI and OCII genes in transgenic potato
 (Solanum tuberosum L.) for resistance to Colorado potato beetle
 (Leptinotarsa decemlineata Say)
IS  - 3
VL  - 198
DO  - 10.1007/s10681-014-1119-z
SP  - 425
EP  - 438
ER  - 

@article{
author = "Cingel, Aleksandar and Savić, Jelena and Ćosić, Tatjana and Zdravković-Korać, Snežana and Momčilović, Ivana and Smigocki, Ann and Ninković, Slavica",
year = "2014",
abstract = "Proteinase inhibitors oryzacystatins I and II (OCI and OCII) have shown
   potential in controlling pests that utilize cysteine proteinases for
   protein digestion. In order to achieve an additive effect against
   Colorado potato beetle (CPB, Leptinotarsa decemlineata Say) digestive
   proteinases, we combined two individual OC genes in potato (Solanum
   tuberosum L.) cultivars Desiree, Dragaevka and Jelica. The OC genes were
   stacked into the potato genome by simultaneous co-transformation with
   both OCI and OCII genes or by sequential re-transformation of an
   OCI-expressing transgenic line with an OCII gene. Low constitutive and
   high wound-induced transcript levels of both OCI and OCII genes,
   directed by the inducible pin2 promoter, were detected in all doubly
   transformed lines from all three cultivars. Both recombinant OCs,
   detected by immunoblot, were in an active conformation as shown by their
   strong papain inhibitory activity. Although no significant differences
   in CPB larval mortality were observed when larvae fed on OCI/OCII
   transformed or control potato foliage, expression of both OCI and OCII
   in potato plants caused a reduction in CPB adult body weight and in
   weight of foliage consumed, indicating an additive effect. Overall,
   these results show that pyramiding of the OCI and OCII genes could be an
   effective strategy for breeding cultivars exhibiting elevated levels of
   resistance to CPB.",
journal = "Euphytica",
title = "Pyramiding rice cystatin OCI and OCII genes in transgenic potato
 (Solanum tuberosum L.) for resistance to Colorado potato beetle
 (Leptinotarsa decemlineata Say)",
number = "3",
volume = "198",
doi = "10.1007/s10681-014-1119-z",
pages = "425-438"
}

Cingel, A., Savić, J., Ćosić, T., Zdravković-Korać, S., Momčilović, I., Smigocki, A.,& Ninković, S.. (2014). Pyramiding rice cystatin OCI and OCII genes in transgenic potato
 (Solanum tuberosum L.) for resistance to Colorado potato beetle
 (Leptinotarsa decemlineata Say). in Euphytica, 198(3), 425-438.
https://doi.org/10.1007/s10681-014-1119-z

Cingel A, Savić J, Ćosić T, Zdravković-Korać S, Momčilović I, Smigocki A, Ninković S. Pyramiding rice cystatin OCI and OCII genes in transgenic potato
 (Solanum tuberosum L.) for resistance to Colorado potato beetle
 (Leptinotarsa decemlineata Say). in Euphytica. 2014;198(3):425-438.
doi:10.1007/s10681-014-1119-z .

Cingel, Aleksandar, Savić, Jelena, Ćosić, Tatjana, Zdravković-Korać, Snežana, Momčilović, Ivana, Smigocki, Ann, Ninković, Slavica, "Pyramiding rice cystatin OCI and OCII genes in transgenic potato
 (Solanum tuberosum L.) for resistance to Colorado potato beetle
 (Leptinotarsa decemlineata Say)" in Euphytica, 198, no. 3 (2014):425-438,
https://doi.org/10.1007/s10681-014-1119-z . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dragićević, Ivana
AU  - Pantelić, D.
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/298
AB  - We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.
T2  - Archives of Biological Sciences
T1  - Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
IS  - 1
VL  - 64
SP  - 135
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_298
ER  - 

@article{
author = "Savić, Jelena and Dragićević, Ivana and Pantelić, D. and Oljača, Jasmina and Momčilović, Ivana",
year = "2012, 2012",
abstract = "We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.",
journal = "Archives of Biological Sciences",
title = "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)",
number = "1",
volume = "64",
pages = "135-144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_298"
}

Savić, J., Dragićević, I., Pantelić, D., Oljača, J.,& Momčilović, I.. (2012). Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences, 64(1), 135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298

Savić J, Dragićević I, Pantelić D, Oljača J, Momčilović I. Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences. 2012;64(1):135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298 .

Savić, Jelena, Dragićević, Ivana, Pantelić, D., Oljača, Jasmina, Momčilović, Ivana, "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)" in Archives of Biological Sciences, 64, no. 1 (2012):135-144,
https://hdl.handle.net/21.15107/rcub_ibiss_298 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Dragičević, Milan B.
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Maksimović, Vuk M
AU  - Momčilović, Ivana
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1110
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
IS  - 13
VL  - 169
SP  - 93
EP  - 1289
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1110
ER  - 

@article{
author = "Mišić, Danijela and Dragičević, Milan B. and Šiler, Branislav and Nestorović Živković, Jasmina and Maksimović, Vuk M and Momčilović, Ivana and Nikolić, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
number = "13",
volume = "169",
pages = "93-1289",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1110"
}

Mišić, D., Dragičević, M. B., Šiler, B., Nestorović Živković, J., Maksimović, V. M., Momčilović, I.,& Nikolić, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology, 169(13), 93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110

Mišić D, Dragičević MB, Šiler B, Nestorović Živković J, Maksimović VM, Momčilović I, Nikolić M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

Mišić, Danijela, Dragičević, Milan B., Šiler, Branislav, Nestorović Živković, Jasmina, Maksimović, Vuk M, Momčilović, Ivana, Nikolić, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):93-1289,
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

TY  - JOUR
AU  - Živković, Suzana
AU  - Popović, M.
AU  - Dragišić-Maksimović, Jelena
AU  - Momčilović, Ivana
AU  - Grubišić, D.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/266
AB  - Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated state. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isoforms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.
T2  - Archives of Biological Sciences
T1  - Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.
IS  - 4
VL  - 62
SP  - 1071
EP  - 1081
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_266
ER  - 

@article{
author = "Živković, Suzana and Popović, M. and Dragišić-Maksimović, Jelena and Momčilović, Ivana and Grubišić, D.",
year = "2010, 2010",
abstract = "Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated state. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isoforms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.",
journal = "Archives of Biological Sciences",
title = "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.",
number = "4",
volume = "62",
pages = "1071-1081",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_266"
}

Živković, S., Popović, M., Dragišić-Maksimović, J., Momčilović, I.,& Grubišić, D.. (2010). Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.. in Archives of Biological Sciences, 62(4), 1071-1081.
https://hdl.handle.net/21.15107/rcub_ibiss_266

Živković S, Popović M, Dragišić-Maksimović J, Momčilović I, Grubišić D. Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.. in Archives of Biological Sciences. 2010;62(4):1071-1081.
https://hdl.handle.net/21.15107/rcub_ibiss_266 .

Živković, Suzana, Popović, M., Dragišić-Maksimović, Jelena, Momčilović, Ivana, Grubišić, D., "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L." in Archives of Biological Sciences, 62, no. 4 (2010):1071-1081,
https://hdl.handle.net/21.15107/rcub_ibiss_266 .