TY  - JOUR
AU  - Bojović, Katarina
AU  - Stanković, Biljana
AU  - Kotur, Nikola
AU  - Krstić Milošević, Dijana
AU  - Gašić, Vladimir
AU  - Pavlović, Sonja
AU  - Zukić, Branka
AU  - Ignjatović, Đurđica
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/1028415X.2017.1352121
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2823
AB  - Gastrointestinal disturbances, nutritional deficiencies, and food intolerances are frequently observed in children with neurodevelopmental disorders (NDD). To reveal possible association of celiac disease risk variants (HLA-DQ), lactose intolerance associated variant (LCT-13910C > T) as well as variant associated with vitamin D function (VDR FokI) with NDD, polymerase chain reaction-based methodology was used. Additionally, intestinal peptide permeability was estimated in NDD patients and healthy children by measuring the level of peptides in urine using high-performance liquid chromatography. Levels of opioid peptides, casomorphin 8, and gluten exorphin C were significantly elevated in urine samples of NDD patients (P = 0.004 and P = 0.005, respectively), but no association of genetic risk variants for celiac disease and lactose intolerance with NDD was found. Our results indicate that increased intestinal peptide permeability observed in analyzed NDD patients is not associated with genetic predictors of celiac disease or lactose intolerance. We have also found that FF genotype of VDR FokI and lower serum levels of vitamin D (25-OH) showed association with childhood autism (CHA), a subgroup of NDD. We hypothesize that vitamin D might be important for the development of CHA.
T2  - Nutritional Neuroscience
T1  - Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders
IS  - 1
VL  - 22
DO  - 10.1080/1028415X.2017.1352121
SP  - 40
EP  - 50
ER  - 

@article{
author = "Bojović, Katarina and Stanković, Biljana and Kotur, Nikola and Krstić Milošević, Dijana and Gašić, Vladimir and Pavlović, Sonja and Zukić, Branka and Ignjatović, Đurđica",
year = "2019",
abstract = "Gastrointestinal disturbances, nutritional deficiencies, and food intolerances are frequently observed in children with neurodevelopmental disorders (NDD). To reveal possible association of celiac disease risk variants (HLA-DQ), lactose intolerance associated variant (LCT-13910C > T) as well as variant associated with vitamin D function (VDR FokI) with NDD, polymerase chain reaction-based methodology was used. Additionally, intestinal peptide permeability was estimated in NDD patients and healthy children by measuring the level of peptides in urine using high-performance liquid chromatography. Levels of opioid peptides, casomorphin 8, and gluten exorphin C were significantly elevated in urine samples of NDD patients (P = 0.004 and P = 0.005, respectively), but no association of genetic risk variants for celiac disease and lactose intolerance with NDD was found. Our results indicate that increased intestinal peptide permeability observed in analyzed NDD patients is not associated with genetic predictors of celiac disease or lactose intolerance. We have also found that FF genotype of VDR FokI and lower serum levels of vitamin D (25-OH) showed association with childhood autism (CHA), a subgroup of NDD. We hypothesize that vitamin D might be important for the development of CHA.",
journal = "Nutritional Neuroscience",
title = "Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders",
number = "1",
volume = "22",
doi = "10.1080/1028415X.2017.1352121",
pages = "40-50"
}

Bojović, K., Stanković, B., Kotur, N., Krstić Milošević, D., Gašić, V., Pavlović, S., Zukić, B.,& Ignjatović, Đ.. (2019). Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders. in Nutritional Neuroscience, 22(1), 40-50.
https://doi.org/10.1080/1028415X.2017.1352121

Bojović K, Stanković B, Kotur N, Krstić Milošević D, Gašić V, Pavlović S, Zukić B, Ignjatović Đ. Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders. in Nutritional Neuroscience. 2019;22(1):40-50.
doi:10.1080/1028415X.2017.1352121 .

Bojović, Katarina, Stanković, Biljana, Kotur, Nikola, Krstić Milošević, Dijana, Gašić, Vladimir, Pavlović, Sonja, Zukić, Branka, Ignjatović, Đurđica, "Genetic predictors of celiac disease, lactose intolerance, and vitamin D function and presence of peptide morphins in urine of children with neurodevelopmental disorders" in Nutritional Neuroscience, 22, no. 1 (2019):40-50,
https://doi.org/10.1080/1028415X.2017.1352121 . .

TY  - JOUR
AU  - Marković, Jelena
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Karan-Djurašević, Teodora
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Pavlović, Sonja
AU  - Poznanović, Goran
AU  - Vidaković, Melita
PY  - 2013
UR  - http://www.ncbi.nlm.nih.gov/pubmed/23555743
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC3608566
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3186
AB  - Despite significant progress, the molecular mechanisms responsible for pancreatic beta cell depletion and development of diabetes remain poorly defined. At present, there is no preventive measure against diabetes. The positive impact of CXCL12 expression on the pancreatic beta cell prosurvival phenotype initiated this study. Our aim was to provide novel insight into the regulation of rat CXCL12 gene (Cxcl12) transcription. The roles of poly(ADP-ribose) polymerase-1 (PARP-1) and transcription factor Yin Yang 1 (YY1) in Cxcl12 transcription were studied by examining their in vitro and in vivo binding affinities for the Cxcl12 promoter in a pancreatic beta cell line by the electrophoretic mobility shift assay and chromatin immunoprecipitation. The regulatory activities of PARP-1 and YY1 were assessed in transfection experiments using a reporter vector with a Cxcl12 promoter sequence driving luciferase gene expression. Experimental evidence for PARP-1 and YY1 revealed their trans-acting potential, wherein PARP-1 displayed an inhibitory, and YY1 a strong activating effect on Cxcl12 transcription. Streptozotocin (STZ)-induced general toxicity in pancreatic beta cells was followed by changes in Cxcl12 promoter regulation. PARP-1 binding to the Cxcl12 promoter during basal and in STZ-compromised conditions led us to conclude that PARP-1 regulates constitutive Cxcl12 expression. During the early stage of oxidative stress, YY1 exhibited less affinity toward the Cxcl12 promoter while PARP-1 displayed strong binding. These interactions were accompanied by Cxcl12 downregulation. In the later stages of oxidative stress and intensive pancreatic beta cell injury, YY1 was highly expressed and firmly bound to Cxcl12 promoter in contrast to PARP-1. These interactions resulted in higher Cxcl12 expression. The observed ability of PARP-1 to downregulate, and of YY1 to upregulate Cxcl12 promoter activity anticipates corresponding effects in the natural context where the functional interplay of these proteins could finely balance Cxcl12 transcription.
PB  - San Francisco: Public Library Science
T2  - PLOS One
T1  - PARP-1 and YY1 Are Important Novel Regulators of CXCL12 Gene Transcription in Rat Pancreatic Beta Cells
IS  - 3
VL  - 8
DO  - 10.1371/journal.pone.0059679
SP  - e59679
ER  - 

@article{
author = "Marković, Jelena and Grdović, Nevena and Dinić, Svetlana and Karan-Djurašević, Teodora and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Pavlović, Sonja and Poznanović, Goran and Vidaković, Melita",
year = "2013",
abstract = "Despite significant progress, the molecular mechanisms responsible for pancreatic beta cell depletion and development of diabetes remain poorly defined. At present, there is no preventive measure against diabetes. The positive impact of CXCL12 expression on the pancreatic beta cell prosurvival phenotype initiated this study. Our aim was to provide novel insight into the regulation of rat CXCL12 gene (Cxcl12) transcription. The roles of poly(ADP-ribose) polymerase-1 (PARP-1) and transcription factor Yin Yang 1 (YY1) in Cxcl12 transcription were studied by examining their in vitro and in vivo binding affinities for the Cxcl12 promoter in a pancreatic beta cell line by the electrophoretic mobility shift assay and chromatin immunoprecipitation. The regulatory activities of PARP-1 and YY1 were assessed in transfection experiments using a reporter vector with a Cxcl12 promoter sequence driving luciferase gene expression. Experimental evidence for PARP-1 and YY1 revealed their trans-acting potential, wherein PARP-1 displayed an inhibitory, and YY1 a strong activating effect on Cxcl12 transcription. Streptozotocin (STZ)-induced general toxicity in pancreatic beta cells was followed by changes in Cxcl12 promoter regulation. PARP-1 binding to the Cxcl12 promoter during basal and in STZ-compromised conditions led us to conclude that PARP-1 regulates constitutive Cxcl12 expression. During the early stage of oxidative stress, YY1 exhibited less affinity toward the Cxcl12 promoter while PARP-1 displayed strong binding. These interactions were accompanied by Cxcl12 downregulation. In the later stages of oxidative stress and intensive pancreatic beta cell injury, YY1 was highly expressed and firmly bound to Cxcl12 promoter in contrast to PARP-1. These interactions resulted in higher Cxcl12 expression. The observed ability of PARP-1 to downregulate, and of YY1 to upregulate Cxcl12 promoter activity anticipates corresponding effects in the natural context where the functional interplay of these proteins could finely balance Cxcl12 transcription.",
publisher = "San Francisco: Public Library Science",
journal = "PLOS One",
title = "PARP-1 and YY1 Are Important Novel Regulators of CXCL12 Gene Transcription in Rat Pancreatic Beta Cells",
number = "3",
volume = "8",
doi = "10.1371/journal.pone.0059679",
pages = "e59679"
}

Marković, J., Grdović, N., Dinić, S., Karan-Djurašević, T., Uskoković, A., Arambašić Jovanović, J., Mihailović, M., Pavlović, S., Poznanović, G.,& Vidaković, M.. (2013). PARP-1 and YY1 Are Important Novel Regulators of CXCL12 Gene Transcription in Rat Pancreatic Beta Cells. in PLOS One
San Francisco: Public Library Science., 8(3), e59679.
https://doi.org/10.1371/journal.pone.0059679

Marković J, Grdović N, Dinić S, Karan-Djurašević T, Uskoković A, Arambašić Jovanović J, Mihailović M, Pavlović S, Poznanović G, Vidaković M. PARP-1 and YY1 Are Important Novel Regulators of CXCL12 Gene Transcription in Rat Pancreatic Beta Cells. in PLOS One. 2013;8(3):e59679.
doi:10.1371/journal.pone.0059679 .

Marković, Jelena, Grdović, Nevena, Dinić, Svetlana, Karan-Djurašević, Teodora, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Pavlović, Sonja, Poznanović, Goran, Vidaković, Melita, "PARP-1 and YY1 Are Important Novel Regulators of CXCL12 Gene Transcription in Rat Pancreatic Beta Cells" in PLOS One, 8, no. 3 (2013):e59679,
https://doi.org/10.1371/journal.pone.0059679 . .