TY  - CONF
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Miljić, Dragana
AU  - Ćirić, Jasmina
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Köhrle, Josef
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6206
AB  - Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.
PB  - Bioscientifica
C3  - 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
T1  - Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats
DO  - 10.1530/endoabs.56.gp246
SP  - GP246
ER  - 

@conference{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Miljić, Dragana and Ćirić, Jasmina and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Köhrle, Josef and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.",
publisher = "Bioscientifica",
journal = "20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain",
title = "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats",
doi = "10.1530/endoabs.56.gp246",
pages = "GP246"
}

Šošić-Jurjević, B., Lütjohann, D., Miljić, D., Ćirić, J., Trifunović, S., Ajdžanović, V., Filipović, B., Köhrle, J., Ušćebrka, G.,& Milošević, V.. (2018). Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
Bioscientifica., GP246.
https://doi.org/10.1530/endoabs.56.gp246

Šošić-Jurjević B, Lütjohann D, Miljić D, Ćirić J, Trifunović S, Ajdžanović V, Filipović B, Köhrle J, Ušćebrka G, Milošević V. Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain. 2018;:GP246.
doi:10.1530/endoabs.56.gp246 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Miljić, Dragana, Ćirić, Jasmina, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Köhrle, Josef, Ušćebrka, Gordana, Milošević, Verica, "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats" in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain (2018):GP246,
https://doi.org/10.1530/endoabs.56.gp246 . .

TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Vanmierlo, Tim
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Lončarević-Vasiljković, Nataša
AU  - Tesić, Vesna T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Lutjohann, Dieter
AU  - Kanazir, Selma
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/953
AB  - Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.
T2  - Lipids
T1  - Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver
IS  - 11
VL  - 48
SP  - 5
EP  - 1077
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_953
ER  - 

@article{
author = "Smiljanić, Kosara and Vanmierlo, Tim and Mladenović, Aleksandra and Perović, Milka and Lončarević-Vasiljković, Nataša and Tesić, Vesna T and Rakić, Ljubisav and Ruždijić, Sabera and Lutjohann, Dieter and Kanazir, Selma",
year = "2013",
abstract = "Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.",
journal = "Lipids",
title = "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver",
number = "11",
volume = "48",
pages = "5-1077",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_953"
}

Smiljanić, K., Vanmierlo, T., Mladenović, A., Perović, M., Lončarević-Vasiljković, N., Tesić, V. T., Rakić, L., Ruždijić, S., Lutjohann, D.,& Kanazir, S.. (2013). Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids, 48(11), 5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953

Smiljanić K, Vanmierlo T, Mladenović A, Perović M, Lončarević-Vasiljković N, Tesić VT, Rakić L, Ruždijić S, Lutjohann D, Kanazir S. Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids. 2013;48(11):5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953 .

Smiljanić, Kosara, Vanmierlo, Tim, Mladenović, Aleksandra, Perović, Milka, Lončarević-Vasiljković, Nataša, Tesić, Vesna T, Rakić, Ljubisav, Ruždijić, Sabera, Lutjohann, Dieter, Kanazir, Selma, "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver" in Lipids, 48, no. 11 (2013):5-1077,
https://hdl.handle.net/21.15107/rcub_ibiss_953 .