TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Stupar, Alena
AU  - Bulut, Gizem
AU  - Senkardes, Ismail
AU  - Dogan, Ahmet
AU  - Sinan, Kouadio Ibrahime
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahiri
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://doi.org/10.1016/j.indcrop.2020.112202
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3609
AB  - Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.
T2  - Industrial Crops & Products
T1  - Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip
IS  - April 2020
VL  - 146
DO  - 10.1016/j.indcrop.2020.112202
SP  - 112202
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Stupar, Alena and Bulut, Gizem and Senkardes, Ismail and Dogan, Ahmet and Sinan, Kouadio Ibrahime and Uysal, Sengul and Aumeeruddy-Elalfi, Zaahiri and Aktumsek, Abdurrahman and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.",
journal = "Industrial Crops & Products",
title = "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip",
number = "April 2020",
volume = "146",
doi = "10.1016/j.indcrop.2020.112202",
pages = "112202"
}

Zengin, G., Cvetanović, A., Gašić, U., Stupar, A., Bulut, G., Senkardes, I., Dogan, A., Sinan, K. I., Uysal, S., Aumeeruddy-Elalfi, Z., Aktumsek, A.,& Mahomoodally, M. F.. (2020). Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products, 146(April 2020), 112202.
https://doi.org/10.1016/j.indcrop.2020.112202

Zengin G, Cvetanović A, Gašić U, Stupar A, Bulut G, Senkardes I, Dogan A, Sinan KI, Uysal S, Aumeeruddy-Elalfi Z, Aktumsek A, Mahomoodally MF. Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products. 2020;146(April 2020):112202.
doi:10.1016/j.indcrop.2020.112202 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Stupar, Alena, Bulut, Gizem, Senkardes, Ismail, Dogan, Ahmet, Sinan, Kouadio Ibrahime, Uysal, Sengul, Aumeeruddy-Elalfi, Zaahiri, Aktumsek, Abdurrahman, Mahomoodally, Mohamad Fawzi, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip" in Industrial Crops & Products, 146, no. April 2020 (2020):112202,
https://doi.org/10.1016/j.indcrop.2020.112202 . .

TY  - JOUR
AU  - Uysal, Sengul
AU  - Senkardes, Ismail
AU  - Mollica, Adriano
AU  - Zengin, Gokhan
AU  - Bulut, Gizem
AU  - Dogan, Ahmet
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Mahomoodally, Fawzi M
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/07391102.2018.1506361
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3194
AB  - Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.
T2  - Journal of Biomolecular Structure and Dynamics
T1  - Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.
IS  - 12
VL  - 37
DO  - 10.1080/07391102.2018.1506361
SP  - 3269
EP  - 3281
ER  - 

@article{
author = "Uysal, Sengul and Senkardes, Ismail and Mollica, Adriano and Zengin, Gokhan and Bulut, Gizem and Dogan, Ahmet and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Mahomoodally, Fawzi M",
year = "2019",
abstract = "Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.",
journal = "Journal of Biomolecular Structure and Dynamics",
title = "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.",
number = "12",
volume = "37",
doi = "10.1080/07391102.2018.1506361",
pages = "3269-3281"
}

Uysal, S., Senkardes, I., Mollica, A., Zengin, G., Bulut, G., Dogan, A., Glamočlija, J., Soković, M., Lobine, D.,& Mahomoodally, F. M.. (2019). Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics, 37(12), 3269-3281.
https://doi.org/10.1080/07391102.2018.1506361

Uysal S, Senkardes I, Mollica A, Zengin G, Bulut G, Dogan A, Glamočlija J, Soković M, Lobine D, Mahomoodally FM. Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics. 2019;37(12):3269-3281.
doi:10.1080/07391102.2018.1506361 .

Uysal, Sengul, Senkardes, Ismail, Mollica, Adriano, Zengin, Gokhan, Bulut, Gizem, Dogan, Ahmet, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Mahomoodally, Fawzi M, "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L." in Journal of Biomolecular Structure and Dynamics, 37, no. 12 (2019):3269-3281,
https://doi.org/10.1080/07391102.2018.1506361 . .