TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Fiorentino, Antonio
AU  - Vuotto, Chiara
AU  - Mahomoodally, Mohamad Fawzi
AU  - Jugreet, Sharmeen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Etienne, Ouattara Katinan
AU  - Pereira, Catarina Guerreiro
AU  - Custódio, Luísa
AU  - Rengasamy, Kannan R.R.
AU  - D'Abrosca, Brigida
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S221242922200219X
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4985
AB  - Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy, especially in African countries. In the present study, ethyl acetate, methanolic and water extracts of different parts of (fruits, leaves, stem barks) P. thonningii were evaluated for their bioactive contents, enzyme inhibitory, antioxidant and antimicrobial activities. Antioxidant potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g), radical scavenging assays (DPPH: 20.39–471.28 mg TE/g; ABTS: 42.43–654.14 mg TE/g); reducing assays (CUPRAC: 102.98–1207.42 mg TE/g; FRAP: 45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g). In particular, the methanolic and water extracts showed better antioxidant potential. Except for a few, the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g), butyrylcholinesterase (0.83–13.80 mg GALAE/g), tyrosinase (4.03–143.86 mg KAE/g), amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g). Additionally, all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration: 0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration: 0.04–0.61 mg/ml) effects. Indeed, the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii
DO  - 10.1016/j.fbio.2022.101760
SP  - 101760
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Zengin, Gokhan and Fiorentino, Antonio and Vuotto, Chiara and Mahomoodally, Mohamad Fawzi and Jugreet, Sharmeen and Glamočlija, Jasmina and Soković, Marina and Etienne, Ouattara Katinan and Pereira, Catarina Guerreiro and Custódio, Luísa and Rengasamy, Kannan R.R. and D'Abrosca, Brigida",
year = "2022",
abstract = "Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy, especially in African countries. In the present study, ethyl acetate, methanolic and water extracts of different parts of (fruits, leaves, stem barks) P. thonningii were evaluated for their bioactive contents, enzyme inhibitory, antioxidant and antimicrobial activities. Antioxidant potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g), radical scavenging assays (DPPH: 20.39–471.28 mg TE/g; ABTS: 42.43–654.14 mg TE/g); reducing assays (CUPRAC: 102.98–1207.42 mg TE/g; FRAP: 45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g). In particular, the methanolic and water extracts showed better antioxidant potential. Except for a few, the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g), butyrylcholinesterase (0.83–13.80 mg GALAE/g), tyrosinase (4.03–143.86 mg KAE/g), amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g). Additionally, all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration: 0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration: 0.04–0.61 mg/ml) effects. Indeed, the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii",
doi = "10.1016/j.fbio.2022.101760",
pages = "101760"
}

Sinan, K. I., Zengin, G., Fiorentino, A., Vuotto, C., Mahomoodally, M. F., Jugreet, S., Glamočlija, J., Soković, M., Etienne, O. K., Pereira, C. G., Custódio, L., Rengasamy, K. R.R.,& D'Abrosca, B.. (2022). Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii. in Food Bioscience
Elsevier Ltd., 101760.
https://doi.org/10.1016/j.fbio.2022.101760

Sinan KI, Zengin G, Fiorentino A, Vuotto C, Mahomoodally MF, Jugreet S, Glamočlija J, Soković M, Etienne OK, Pereira CG, Custódio L, Rengasamy KR, D'Abrosca B. Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii. in Food Bioscience. 2022;:101760.
doi:10.1016/j.fbio.2022.101760 .

Sinan, Kouadio Ibrahime, Zengin, Gokhan, Fiorentino, Antonio, Vuotto, Chiara, Mahomoodally, Mohamad Fawzi, Jugreet, Sharmeen, Glamočlija, Jasmina, Soković, Marina, Etienne, Ouattara Katinan, Pereira, Catarina Guerreiro, Custódio, Luísa, Rengasamy, Kannan R.R., D'Abrosca, Brigida, "Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii" in Food Bioscience (2022):101760,
https://doi.org/10.1016/j.fbio.2022.101760 . .

TY  - JOUR
AU  - Senizza, Biancamaria
AU  - Rocchetti, Gabriele
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Etienne, Ouattara Katinan
AU  - Lucini, Luigi
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4171
AB  - Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties
VL  - 41
DO  - 10.1016/j.fbio.2021.100974
SP  - 100974
ER  - 

@article{
author = "Senizza, Biancamaria and Rocchetti, Gabriele and Sinan, Kouadio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Etienne, Ouattara Katinan and Lucini, Luigi",
year = "2021",
abstract = "Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties",
volume = "41",
doi = "10.1016/j.fbio.2021.100974",
pages = "100974"
}

Senizza, B., Rocchetti, G., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Glamočlija, J., Soković, M., Lobine, D., Etienne, O. K.,& Lucini, L.. (2021). The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience
Elsevier Ltd., 41, 100974.
https://doi.org/10.1016/j.fbio.2021.100974

Senizza B, Rocchetti G, Sinan KI, Zengin G, Mahomoodally MF, Glamočlija J, Soković M, Lobine D, Etienne OK, Lucini L. The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience. 2021;41:100974.
doi:10.1016/j.fbio.2021.100974 .

Senizza, Biancamaria, Rocchetti, Gabriele, Sinan, Kouadio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Etienne, Ouattara Katinan, Lucini, Luigi, "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties" in Food Bioscience, 41 (2021):100974,
https://doi.org/10.1016/j.fbio.2021.100974 . .

TY  - JOUR
AU  - Chiavaroli, Annalisa
AU  - Sinan, Koaudio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Bibi Sadeer, Nabeelah
AU  - Etienne, Ouattara Katinan
AU  - Cziáky, Zoltán
AU  - Jekő, József
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Recinella, Lucia
AU  - Brunetti, Luigi
AU  - Leone, Sheila
AU  - Abdallah, Hassan H.
AU  - Angelini, Paola
AU  - Angeles Flores, Giancarlo
AU  - Venanzoni, Roberto
AU  - Menghini, Luigi
AU  - Orlando, Giustino
AU  - Ferrante, Claudio
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/6/533
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3719
AB  - Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.
PB  - MDPI AG
T2  - Antioxidants
T1  - Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents
IS  - 6
VL  - 9
DO  - 10.3390/antiox9060533
SP  - 533
ER  - 

@article{
author = "Chiavaroli, Annalisa and Sinan, Koaudio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Bibi Sadeer, Nabeelah and Etienne, Ouattara Katinan and Cziáky, Zoltán and Jekő, József and Glamočlija, Jasmina and Soković, Marina and Recinella, Lucia and Brunetti, Luigi and Leone, Sheila and Abdallah, Hassan H. and Angelini, Paola and Angeles Flores, Giancarlo and Venanzoni, Roberto and Menghini, Luigi and Orlando, Giustino and Ferrante, Claudio",
year = "2020",
abstract = "Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents",
number = "6",
volume = "9",
doi = "10.3390/antiox9060533",
pages = "533"
}

Chiavaroli, A., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Bibi Sadeer, N., Etienne, O. K., Cziáky, Z., Jekő, J., Glamočlija, J., Soković, M., Recinella, L., Brunetti, L., Leone, S., Abdallah, H. H., Angelini, P., Angeles Flores, G., Venanzoni, R., Menghini, L., Orlando, G.,& Ferrante, C.. (2020). Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants
MDPI AG., 9(6), 533.
https://doi.org/10.3390/antiox9060533

Chiavaroli A, Sinan KI, Zengin G, Mahomoodally MF, Bibi Sadeer N, Etienne OK, Cziáky Z, Jekő J, Glamočlija J, Soković M, Recinella L, Brunetti L, Leone S, Abdallah HH, Angelini P, Angeles Flores G, Venanzoni R, Menghini L, Orlando G, Ferrante C. Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants. 2020;9(6):533.
doi:10.3390/antiox9060533 .

Chiavaroli, Annalisa, Sinan, Koaudio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Bibi Sadeer, Nabeelah, Etienne, Ouattara Katinan, Cziáky, Zoltán, Jekő, József, Glamočlija, Jasmina, Soković, Marina, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Abdallah, Hassan H., Angelini, Paola, Angeles Flores, Giancarlo, Venanzoni, Roberto, Menghini, Luigi, Orlando, Giustino, Ferrante, Claudio, "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents" in Antioxidants, 9, no. 6 (2020):533,
https://doi.org/10.3390/antiox9060533 . .