TY  - JOUR
AU  - Uysal, Sengul
AU  - Senkardes, Ismail
AU  - Mollica, Adriano
AU  - Zengin, Gokhan
AU  - Bulut, Gizem
AU  - Dogan, Ahmet
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Mahomoodally, Fawzi M
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/07391102.2018.1506361
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3194
AB  - Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.
T2  - Journal of Biomolecular Structure and Dynamics
T1  - Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.
IS  - 12
VL  - 37
DO  - 10.1080/07391102.2018.1506361
SP  - 3269
EP  - 3281
ER  - 

@article{
author = "Uysal, Sengul and Senkardes, Ismail and Mollica, Adriano and Zengin, Gokhan and Bulut, Gizem and Dogan, Ahmet and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Mahomoodally, Fawzi M",
year = "2019",
abstract = "Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.",
journal = "Journal of Biomolecular Structure and Dynamics",
title = "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.",
number = "12",
volume = "37",
doi = "10.1080/07391102.2018.1506361",
pages = "3269-3281"
}

Uysal, S., Senkardes, I., Mollica, A., Zengin, G., Bulut, G., Dogan, A., Glamočlija, J., Soković, M., Lobine, D.,& Mahomoodally, F. M.. (2019). Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics, 37(12), 3269-3281.
https://doi.org/10.1080/07391102.2018.1506361

Uysal S, Senkardes I, Mollica A, Zengin G, Bulut G, Dogan A, Glamočlija J, Soković M, Lobine D, Mahomoodally FM. Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics. 2019;37(12):3269-3281.
doi:10.1080/07391102.2018.1506361 .

Uysal, Sengul, Senkardes, Ismail, Mollica, Adriano, Zengin, Gokhan, Bulut, Gizem, Dogan, Ahmet, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Mahomoodally, Fawzi M, "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L." in Journal of Biomolecular Structure and Dynamics, 37, no. 12 (2019):3269-3281,
https://doi.org/10.1080/07391102.2018.1506361 . .