TY  - JOUR
AU  - Savić, Danijela
AU  - Stanković, Tijana
AU  - Lavrnja, Irena
AU  - Podolski-Renić, Ana
AU  - Banković, Jasna
AU  - Peković, Sanja
AU  - Stojiljković, Mirjana
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Pešić, Milica
PY  - 2015
UR  - http://www.degruyter.com/view/j/motth.2015.1.issue-1/motth-2015-0002/motth-2015-0002.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2633
AB  - Purine nucleoside analogs have been in clinical use for almost 50 years. At the beginning developed as antiviral agents, later their efficacy was demonstrated in cancer treatment, especially hematological malignances. The approval of new purine nucleoside analogs by US Food and Drug Administration (FDA) over the past decade implies that the interest for these drugs still exists. Here, we review new nucleoside analogs that are currently in preclinical or clinical development as anticancer agents. In addition, we highlight the potential for implementation of these drugs in other pathological conditions, particularly in neuroinflammation.
T2  - Molecular inhibitors in targeted therapy
T1  - Purine nucleoside analogs in the therapy of cancer and neuroinflammation
IS  - 1
VL  - 1
DO  - 10.1515/motth-2015-0002
SP  - 3
EP  - 14
ER  - 

@article{
author = "Savić, Danijela and Stanković, Tijana and Lavrnja, Irena and Podolski-Renić, Ana and Banković, Jasna and Peković, Sanja and Stojiljković, Mirjana and Rakić, Ljubisav and Ruždijić, Sabera and Pešić, Milica",
year = "2015",
abstract = "Purine nucleoside analogs have been in clinical use for almost 50 years. At the beginning developed as antiviral agents, later their efficacy was demonstrated in cancer treatment, especially hematological malignances. The approval of new purine nucleoside analogs by US Food and Drug Administration (FDA) over the past decade implies that the interest for these drugs still exists. Here, we review new nucleoside analogs that are currently in preclinical or clinical development as anticancer agents. In addition, we highlight the potential for implementation of these drugs in other pathological conditions, particularly in neuroinflammation.",
journal = "Molecular inhibitors in targeted therapy",
title = "Purine nucleoside analogs in the therapy of cancer and neuroinflammation",
number = "1",
volume = "1",
doi = "10.1515/motth-2015-0002",
pages = "3-14"
}

Savić, D., Stanković, T., Lavrnja, I., Podolski-Renić, A., Banković, J., Peković, S., Stojiljković, M., Rakić, L., Ruždijić, S.,& Pešić, M.. (2015). Purine nucleoside analogs in the therapy of cancer and neuroinflammation. in Molecular inhibitors in targeted therapy, 1(1), 3-14.
https://doi.org/10.1515/motth-2015-0002

Savić D, Stanković T, Lavrnja I, Podolski-Renić A, Banković J, Peković S, Stojiljković M, Rakić L, Ruždijić S, Pešić M. Purine nucleoside analogs in the therapy of cancer and neuroinflammation. in Molecular inhibitors in targeted therapy. 2015;1(1):3-14.
doi:10.1515/motth-2015-0002 .

Savić, Danijela, Stanković, Tijana, Lavrnja, Irena, Podolski-Renić, Ana, Banković, Jasna, Peković, Sanja, Stojiljković, Mirjana, Rakić, Ljubisav, Ruždijić, Sabera, Pešić, Milica, "Purine nucleoside analogs in the therapy of cancer and neuroinflammation" in Molecular inhibitors in targeted therapy, 1, no. 1 (2015):3-14,
https://doi.org/10.1515/motth-2015-0002 . .

TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Vanmierlo, Tim
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Lončarević-Vasiljković, Nataša
AU  - Tesić, Vesna T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Lutjohann, Dieter
AU  - Kanazir, Selma
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/953
AB  - Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.
T2  - Lipids
T1  - Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver
IS  - 11
VL  - 48
SP  - 5
EP  - 1077
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_953
ER  - 

@article{
author = "Smiljanić, Kosara and Vanmierlo, Tim and Mladenović, Aleksandra and Perović, Milka and Lončarević-Vasiljković, Nataša and Tesić, Vesna T and Rakić, Ljubisav and Ruždijić, Sabera and Lutjohann, Dieter and Kanazir, Selma",
year = "2013",
abstract = "Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.",
journal = "Lipids",
title = "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver",
number = "11",
volume = "48",
pages = "5-1077",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_953"
}

Smiljanić, K., Vanmierlo, T., Mladenović, A., Perović, M., Lončarević-Vasiljković, N., Tesić, V. T., Rakić, L., Ruždijić, S., Lutjohann, D.,& Kanazir, S.. (2013). Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids, 48(11), 5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953

Smiljanić K, Vanmierlo T, Mladenović A, Perović M, Lončarević-Vasiljković N, Tesić VT, Rakić L, Ruždijić S, Lutjohann D, Kanazir S. Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids. 2013;48(11):5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953 .

Smiljanić, Kosara, Vanmierlo, Tim, Mladenović, Aleksandra, Perović, Milka, Lončarević-Vasiljković, Nataša, Tesić, Vesna T, Rakić, Ljubisav, Ruždijić, Sabera, Lutjohann, Dieter, Kanazir, Selma, "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver" in Lipids, 48, no. 11 (2013):5-1077,
https://hdl.handle.net/21.15107/rcub_ibiss_953 .

TY  - CONF
AU  - Brkić, Predrag D
AU  - Jovanović, Tomislav S
AU  - Krstić, Danijela Z
AU  - Stojiljković, Mirjana B
AU  - Colović, Mirjana B
AU  - Dačić, Sanja
AU  - Mitrović, Ana
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Parbucki, Ana
AU  - Lavrnja, Irena
AU  - Rakić, Ljubisav
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1188
C3  - Brain Injury
T1  - Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury
IS  - 4-5
VL  - 26
EP  - 487
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1188
ER  - 

@conference{
author = "Brkić, Predrag D and Jovanović, Tomislav S and Krstić, Danijela Z and Stojiljković, Mirjana B and Colović, Mirjana B and Dačić, Sanja and Mitrović, Ana and Bjelobaba, Ivana and Savić, Danijela and Parbucki, Ana and Lavrnja, Irena and Rakić, Ljubisav and Peković, Sanja",
year = "2012",
journal = "Brain Injury",
title = "Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury",
number = "4-5",
volume = "26",
pages = "487",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1188"
}

Brkić, P. D., Jovanović, T. S., Krstić, D. Z., Stojiljković, M. B., Colović, M. B., Dačić, S., Mitrović, A., Bjelobaba, I., Savić, D., Parbucki, A., Lavrnja, I., Rakić, L.,& Peković, S.. (2012). Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury. in Brain Injury, 26(4-5).
https://hdl.handle.net/21.15107/rcub_ibiss_1188

Brkić PD, Jovanović TS, Krstić DZ, Stojiljković MB, Colović MB, Dačić S, Mitrović A, Bjelobaba I, Savić D, Parbucki A, Lavrnja I, Rakić L, Peković S. Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury. in Brain Injury. 2012;26(4-5):null-487.
https://hdl.handle.net/21.15107/rcub_ibiss_1188 .

Brkić, Predrag D, Jovanović, Tomislav S, Krstić, Danijela Z, Stojiljković, Mirjana B, Colović, Mirjana B, Dačić, Sanja, Mitrović, Ana, Bjelobaba, Ivana, Savić, Danijela, Parbucki, Ana, Lavrnja, Irena, Rakić, Ljubisav, Peković, Sanja, "Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury" in Brain Injury, 26, no. 4-5 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1188 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Bjelobaba, Ivana
AU  - Dačić, Sanja
AU  - Božić, Iva D.
AU  - Parabučki, Ana B.
AU  - Nedeljković, Nadezda N
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1167
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.
T2  - Journal of Pharmacological Sciences
T1  - The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis
IS  - 3
VL  - 119
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1167
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Bjelobaba, Ivana and Dačić, Sanja and Božić, Iva D. and Parabučki, Ana B. and Nedeljković, Nadezda N and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2012",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.",
journal = "Journal of Pharmacological Sciences",
title = "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis",
number = "3",
volume = "119",
pages = "232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1167"
}

Lavrnja, I., Savić, D., Bjelobaba, I., Dačić, S., Božić, I. D., Parabučki, A. B., Nedeljković, N. N., Peković, S., Rakić, L.,& Stojiljković, M. B.. (2012). The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences, 119(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1167

Lavrnja I, Savić D, Bjelobaba I, Dačić S, Božić ID, Parabučki AB, Nedeljković NN, Peković S, Rakić L, Stojiljković MB. The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences. 2012;119(3):null-232.
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

Lavrnja, Irena, Savić, Danijela, Bjelobaba, Ivana, Dačić, Sanja, Božić, Iva D., Parabučki, Ana B., Nedeljković, Nadezda N, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana B, "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis" in Journal of Pharmacological Sciences, 119, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

TY  - JOUR
AU  - Brkić, Predrag D
AU  - Stojiljković, Mirjana B
AU  - Jovanović, Tomislav S
AU  - Dačić, Sanja
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Bjelobaba, Ivana
AU  - Rakić, Ljubisav
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1146
AB  - Objective: To investigate whether hyperbaric oxygenation (HBO) can improve the recovery of motor functions in rats after suction ablation of the right sensorimotor cortex. Methods: The experimental paradigm implies the following groups: Control animals (C), Control+HBO (CHBO), Sham controls (S), Sham control+HBO (SHBO), Lesion group (L), right sensorimotor cortex was removed by suction, Lesion+HBO (LHBO). Hyperbaric protocol: pressure applied 2.5 atmospheres absolute, for 60 minutes, once a day for 10 days. A beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Expression profiles of growth-associated protein 43 (GAP43) and synaptophysin (SYP) were detected using immunohistochemistry. Results: The LHBO group achieved statistically superior scores in the beam walking test compared to the L group. Additionally, the recovery of muscle strength of the affected hindpaw was significantly enhanced after HBO treatment. Hyperbaric oxygenation induced over-expression of GAP43 and SYP in the neurons surrounding the lesion site. Conclusions: Data presented suggest that hyperbaric oxygen therapy can intensify neuroplastic responses by promoting axonal sprouting and synapse remodelling, which contributes to the recovery of locomotor performances in rats. This provides the perspective for implementation of HBO in clinical strategies for treating traumatic brain injuries.
T2  - Brain Injury
T1  - Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats
IS  - 10
VL  - 26
SP  - 961
EP  - 1284
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1146
ER  - 

@article{
author = "Brkić, Predrag D and Stojiljković, Mirjana B and Jovanović, Tomislav S and Dačić, Sanja and Lavrnja, Irena and Savić, Danijela and Parabučki, Ana B. and Bjelobaba, Ivana and Rakić, Ljubisav and Peković, Sanja",
year = "2012",
abstract = "Objective: To investigate whether hyperbaric oxygenation (HBO) can improve the recovery of motor functions in rats after suction ablation of the right sensorimotor cortex. Methods: The experimental paradigm implies the following groups: Control animals (C), Control+HBO (CHBO), Sham controls (S), Sham control+HBO (SHBO), Lesion group (L), right sensorimotor cortex was removed by suction, Lesion+HBO (LHBO). Hyperbaric protocol: pressure applied 2.5 atmospheres absolute, for 60 minutes, once a day for 10 days. A beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Expression profiles of growth-associated protein 43 (GAP43) and synaptophysin (SYP) were detected using immunohistochemistry. Results: The LHBO group achieved statistically superior scores in the beam walking test compared to the L group. Additionally, the recovery of muscle strength of the affected hindpaw was significantly enhanced after HBO treatment. Hyperbaric oxygenation induced over-expression of GAP43 and SYP in the neurons surrounding the lesion site. Conclusions: Data presented suggest that hyperbaric oxygen therapy can intensify neuroplastic responses by promoting axonal sprouting and synapse remodelling, which contributes to the recovery of locomotor performances in rats. This provides the perspective for implementation of HBO in clinical strategies for treating traumatic brain injuries.",
journal = "Brain Injury",
title = "Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats",
number = "10",
volume = "26",
pages = "961-1284",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1146"
}

Brkić, P. D., Stojiljković, M. B., Jovanović, T. S., Dačić, S., Lavrnja, I., Savić, D., Parabučki, A. B., Bjelobaba, I., Rakić, L.,& Peković, S.. (2012). Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. in Brain Injury, 26(10), 961-1284.
https://hdl.handle.net/21.15107/rcub_ibiss_1146

Brkić PD, Stojiljković MB, Jovanović TS, Dačić S, Lavrnja I, Savić D, Parabučki AB, Bjelobaba I, Rakić L, Peković S. Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. in Brain Injury. 2012;26(10):961-1284.
https://hdl.handle.net/21.15107/rcub_ibiss_1146 .

Brkić, Predrag D, Stojiljković, Mirjana B, Jovanović, Tomislav S, Dačić, Sanja, Lavrnja, Irena, Savić, Danijela, Parabučki, Ana B., Bjelobaba, Ivana, Rakić, Ljubisav, Peković, Sanja, "Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats" in Brain Injury, 26, no. 10 (2012):961-1284,
https://hdl.handle.net/21.15107/rcub_ibiss_1146 .

TY  - JOUR
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Tesić, Vesna T
AU  - Tanić, Nikola T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1414
AB  - Brain aging is related to the numerous structural and functional changes including decreased synaptic plasticity. The beneficial effects of dietary restriction (DR) are well known but insufficiently investigated at the level of plasticity-related markers. Therefore, the aim of this study was to examine the expression profiles of proteins structurally and functionally related to synapses-growth-associated protein 43 (GAP-43), synaptophysin (SPH) and alpha-synuclein (alpha-Syn), in the course of aging and in response to long-term DR. The mRNA and protein levels of three presynaptic proteins were assessed by Real Time RT-PCR and Western blotting in the cortex and hippocampus of young (6-month-old), middle-aged (12-month-old), aged (18-month-old) and old (24-month-old) male Wistar rats fed ad libitum and exposed to DR starting from 6 months of age. We observed that long-term DR modulated age-related transcriptional changes by maintaining stable mRNAs levels in the cortex. No major age-related changes of the protein levels were observed in the cortex, while the specific temporal decline was detected in the hippocampus for all three proteins. The SPH levels were decreased across lifespan (0.8-, 0.8- and 0.6-fold change at 12,18 and 24 months), while the significant decrease of GAP-43 and alpha-Syn protein was detected at 24 months of age (0.6- and 0.7-fold decrease, respectively). Long-term DR eliminated this decline by increasing GAP-43, SPH and alpha-Syn protein levels (1.7-, 1.7- and 1.6-fold, respectively) thus reverting protein levels to the values measured in 6-month-old animals. Specific pattern of changes observed in the hippocampus identifies this structure as more vulnerable to the processes of aging and with a more pronounced response to the DR effects. The observed DR-induced stabilization of the levels of three presynaptic proteins indicates the beneficial effect of DR on age-related decline in the capacity for synaptic plasticity. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat
IS  - 2
VL  - 56
EP  - 255
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1414
ER  - 

@article{
author = "Mladenović, Aleksandra and Perović, Milka and Tesić, Vesna T and Tanić, Nikola T and Rakić, Ljubisav and Ruždijić, Sabera and Kanazir, Selma",
year = "2010",
abstract = "Brain aging is related to the numerous structural and functional changes including decreased synaptic plasticity. The beneficial effects of dietary restriction (DR) are well known but insufficiently investigated at the level of plasticity-related markers. Therefore, the aim of this study was to examine the expression profiles of proteins structurally and functionally related to synapses-growth-associated protein 43 (GAP-43), synaptophysin (SPH) and alpha-synuclein (alpha-Syn), in the course of aging and in response to long-term DR. The mRNA and protein levels of three presynaptic proteins were assessed by Real Time RT-PCR and Western blotting in the cortex and hippocampus of young (6-month-old), middle-aged (12-month-old), aged (18-month-old) and old (24-month-old) male Wistar rats fed ad libitum and exposed to DR starting from 6 months of age. We observed that long-term DR modulated age-related transcriptional changes by maintaining stable mRNAs levels in the cortex. No major age-related changes of the protein levels were observed in the cortex, while the specific temporal decline was detected in the hippocampus for all three proteins. The SPH levels were decreased across lifespan (0.8-, 0.8- and 0.6-fold change at 12,18 and 24 months), while the significant decrease of GAP-43 and alpha-Syn protein was detected at 24 months of age (0.6- and 0.7-fold decrease, respectively). Long-term DR eliminated this decline by increasing GAP-43, SPH and alpha-Syn protein levels (1.7-, 1.7- and 1.6-fold, respectively) thus reverting protein levels to the values measured in 6-month-old animals. Specific pattern of changes observed in the hippocampus identifies this structure as more vulnerable to the processes of aging and with a more pronounced response to the DR effects. The observed DR-induced stabilization of the levels of three presynaptic proteins indicates the beneficial effect of DR on age-related decline in the capacity for synaptic plasticity. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat",
number = "2",
volume = "56",
pages = "255",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1414"
}

Mladenović, A., Perović, M., Tesić, V. T., Tanić, N. T., Rakić, L., Ruždijić, S.,& Kanazir, S.. (2010). Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat. in Neurochemistry International, 56(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1414

Mladenović A, Perović M, Tesić VT, Tanić NT, Rakić L, Ruždijić S, Kanazir S. Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat. in Neurochemistry International. 2010;56(2):null-255.
https://hdl.handle.net/21.15107/rcub_ibiss_1414 .

Mladenović, Aleksandra, Perović, Milka, Tesić, Vesna T, Tanić, Nikola T, Rakić, Ljubisav, Ruždijić, Sabera, Kanazir, Selma, "Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat" in Neurochemistry International, 56, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1414 .

TY  - JOUR
AU  - Laketa, Danijela
AU  - Bjelobaba, Ivana
AU  - Savić, Jasmina Z
AU  - Lavrnja, Irena
AU  - Stojiljković, Mirjana B
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadezda N
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1372
AB  - Biochemical properties of nucleotide pyrophosphatase/phosphodiesterase (NPP) in rat serum have been described by assessing its nucleotide phosphodiesterase activity, using p-nitrophenyl-5'-thymidine monophosphate (p-Nph-5'-TMP) as a substrate. It was demonstrated that NPP activity shares some typical characteristics described for other soluble NPP, such as divalent cation dependence, strong alkaline pH optimum (pH 10.5), inhibition by glycosaminoglycans, and K (m) for p-Nph-5'-TMP hydrolysis of 61.8 +/- A 5.2 mu M. In order to characterize the relation between phosphodiesterase and pyrophosphatase activities of NPP, we have analyzed the effects of different natural nucleotides and nucleotide analogs. ATP, ADP, and AMP competitively inhibited p-Nph-5'-TMP hydrolysis with K (i) values ranging 13-43 mu M. Nucleotide analogs, alpha,beta-metATP, BzATP, 2-MeSATP, and dialATP behaved as competitive inhibitors, whereas alpha,beta-metADP induced mixed inhibition, with K (i) ranging from 2 to 20 mu M. Chromatographic analysis revealed that alpha,beta-metATP, BzATP, and 2-MeSATP were catalytically degraded in the serum, whereas dialATP and alpha,beta-metADP resisted hydrolysis, implying that the former act as substrates and the latter as true competitive inhibitors of serum NPP activity. Since NPP activity is involved in generation, breakdown, and recycling of extracellular adenine nucleotides in the vascular compartment, the results suggest that both hydrolyzable and non-hydrolyzable nucleotide analogs could alter the amplitude and direction of ATP actions and could have potential therapeutic application.
T2  - Molecular and Cellular Biochemistry
T1  - Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum
IS  - 1-2
VL  - 339
EP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1372
ER  - 

@article{
author = "Laketa, Danijela and Bjelobaba, Ivana and Savić, Jasmina Z and Lavrnja, Irena and Stojiljković, Mirjana B and Rakić, Ljubisav and Nedeljković, Nadezda N",
year = "2010",
abstract = "Biochemical properties of nucleotide pyrophosphatase/phosphodiesterase (NPP) in rat serum have been described by assessing its nucleotide phosphodiesterase activity, using p-nitrophenyl-5'-thymidine monophosphate (p-Nph-5'-TMP) as a substrate. It was demonstrated that NPP activity shares some typical characteristics described for other soluble NPP, such as divalent cation dependence, strong alkaline pH optimum (pH 10.5), inhibition by glycosaminoglycans, and K (m) for p-Nph-5'-TMP hydrolysis of 61.8 +/- A 5.2 mu M. In order to characterize the relation between phosphodiesterase and pyrophosphatase activities of NPP, we have analyzed the effects of different natural nucleotides and nucleotide analogs. ATP, ADP, and AMP competitively inhibited p-Nph-5'-TMP hydrolysis with K (i) values ranging 13-43 mu M. Nucleotide analogs, alpha,beta-metATP, BzATP, 2-MeSATP, and dialATP behaved as competitive inhibitors, whereas alpha,beta-metADP induced mixed inhibition, with K (i) ranging from 2 to 20 mu M. Chromatographic analysis revealed that alpha,beta-metATP, BzATP, and 2-MeSATP were catalytically degraded in the serum, whereas dialATP and alpha,beta-metADP resisted hydrolysis, implying that the former act as substrates and the latter as true competitive inhibitors of serum NPP activity. Since NPP activity is involved in generation, breakdown, and recycling of extracellular adenine nucleotides in the vascular compartment, the results suggest that both hydrolyzable and non-hydrolyzable nucleotide analogs could alter the amplitude and direction of ATP actions and could have potential therapeutic application.",
journal = "Molecular and Cellular Biochemistry",
title = "Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum",
number = "1-2",
volume = "339",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1372"
}

Laketa, D., Bjelobaba, I., Savić, J. Z., Lavrnja, I., Stojiljković, M. B., Rakić, L.,& Nedeljković, N. N.. (2010). Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum. in Molecular and Cellular Biochemistry, 339(1-2).
https://hdl.handle.net/21.15107/rcub_ibiss_1372

Laketa D, Bjelobaba I, Savić JZ, Lavrnja I, Stojiljković MB, Rakić L, Nedeljković NN. Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum. in Molecular and Cellular Biochemistry. 2010;339(1-2):null-106.
https://hdl.handle.net/21.15107/rcub_ibiss_1372 .

Laketa, Danijela, Bjelobaba, Ivana, Savić, Jasmina Z, Lavrnja, Irena, Stojiljković, Mirjana B, Rakić, Ljubisav, Nedeljković, Nadezda N, "Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum" in Molecular and Cellular Biochemistry, 339, no. 1-2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1372 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Stojiljković, Mirjana B
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Dačić, Sanja
AU  - Laketa, Danijela
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadezda N
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1473
AB  - During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering local cellular and biochemical responses to injury. Different models of brain injury reveal that the local increase in adenine nucleotides levels is followed by a compensatory up-regulation of ectonucleotidase enzymes that catalyze sequential hydrolysis of ATP to ADP, AMP and adenosine. However, recent studies imply that changes in adenine nucleotides release may also occur in the areas distant from the site of direct damage. Therefore, in the present study we have used the model of cortical stab injury to analyze extracellular ATP, ADP and AMP hydrolysis in the membrane preparations obtained from the brain regions that were not subjected to direct tissue damage. The brain regions analyzed were contralateral cortex, hippocampus, caudate nucleus, thalamus and hypothalamus. It was evidenced that cortical stab injury induced early widespread decrease in AMP hydrolysis in all brain areas tested, except in the hypothalamus, without changes in ATP hydrolysis. These findings imply that brain injury affects global extracellular adenine nucleotide and nucleoside levels, consequently affecting neuronal function in the regions distant to the primary damage.
T2  - General Physiology and Biophysics
T1  - Regional changes in ectonucleotidase activity after cortical stab injury in rat
IS  - null
VL  - 28
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1473
ER  - 

@article{
author = "Bjelobaba, Ivana and Stojiljković, Mirjana B and Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Dačić, Sanja and Laketa, Danijela and Rakić, Ljubisav and Nedeljković, Nadezda N",
year = "2009",
abstract = "During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering local cellular and biochemical responses to injury. Different models of brain injury reveal that the local increase in adenine nucleotides levels is followed by a compensatory up-regulation of ectonucleotidase enzymes that catalyze sequential hydrolysis of ATP to ADP, AMP and adenosine. However, recent studies imply that changes in adenine nucleotides release may also occur in the areas distant from the site of direct damage. Therefore, in the present study we have used the model of cortical stab injury to analyze extracellular ATP, ADP and AMP hydrolysis in the membrane preparations obtained from the brain regions that were not subjected to direct tissue damage. The brain regions analyzed were contralateral cortex, hippocampus, caudate nucleus, thalamus and hypothalamus. It was evidenced that cortical stab injury induced early widespread decrease in AMP hydrolysis in all brain areas tested, except in the hypothalamus, without changes in ATP hydrolysis. These findings imply that brain injury affects global extracellular adenine nucleotide and nucleoside levels, consequently affecting neuronal function in the regions distant to the primary damage.",
journal = "General Physiology and Biophysics",
title = "Regional changes in ectonucleotidase activity after cortical stab injury in rat",
number = "null",
volume = "28",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1473"
}

Bjelobaba, I., Stojiljković, M. B., Lavrnja, I., Savić, D., Peković, S., Dačić, S., Laketa, D., Rakić, L.,& Nedeljković, N. N.. (2009). Regional changes in ectonucleotidase activity after cortical stab injury in rat. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1473

Bjelobaba I, Stojiljković MB, Lavrnja I, Savić D, Peković S, Dačić S, Laketa D, Rakić L, Nedeljković NN. Regional changes in ectonucleotidase activity after cortical stab injury in rat. in General Physiology and Biophysics. 2009;28(null):null-68.
https://hdl.handle.net/21.15107/rcub_ibiss_1473 .

Bjelobaba, Ivana, Stojiljković, Mirjana B, Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Dačić, Sanja, Laketa, Danijela, Rakić, Ljubisav, Nedeljković, Nadezda N, "Regional changes in ectonucleotidase activity after cortical stab injury in rat" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1473 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Nedeljković, Nadezda N
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Dačić, Sanja
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Stojiljković, Mirjana B
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1474
AB  - The role of extracellular purines and purinoreceptors in the pathophysiology of different neurological disorders is the focus of rapidly expanding area of research. Ectonucleotidases are the enzymes with multiple roles in extracellular nucleotides metabolism and regulation of nucleotide-based intercellular signaling. The aim of present study was to investigate the changes in the ATP, ADP and AMP hydrolyzing activities after ribavirin treatment in spinal cord during experimental autoimmune encephalomyelitis (EAE). Our results demonstrate that ribavirin itself had no significant effect on ectoenzyme activities, when tested in vitro and in vivo on spinal cord crude membrane preparation of intact animals. We observed significant increase in ATP, ADP and AMP hydrolyzing activity in the spinal cord crude membrane preparation in EAE animals at 15 days post immunization compared to control animals. The increase was registered at 28 days post immunization, as well. At same time points, ribavirin treatment decreased ATP, ADP and AMP hydrolyzing activity compared to EAE animals. In addition, no significant changes 8 days post immunization was observed between EAE-induced and ribavirin- treated EAE animals and these levels were similar to control level. Thus, we suppose that ribavirin-induced alteration in ectonucleotidase activities is rather due to its suppression of inflammation, than to its direct action on ATP, ADP and AMP hydrolysis.
T2  - General Physiology and Biophysics
T1  - Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis
IS  - null
VL  - 28
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1474
ER  - 

@article{
author = "Lavrnja, Irena and Nedeljković, Nadezda N and Bjelobaba, Ivana and Savić, Danijela and Dačić, Sanja and Peković, Sanja and Rakić, Ljubisav and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Stojiljković, Mirjana B",
year = "2009",
abstract = "The role of extracellular purines and purinoreceptors in the pathophysiology of different neurological disorders is the focus of rapidly expanding area of research. Ectonucleotidases are the enzymes with multiple roles in extracellular nucleotides metabolism and regulation of nucleotide-based intercellular signaling. The aim of present study was to investigate the changes in the ATP, ADP and AMP hydrolyzing activities after ribavirin treatment in spinal cord during experimental autoimmune encephalomyelitis (EAE). Our results demonstrate that ribavirin itself had no significant effect on ectoenzyme activities, when tested in vitro and in vivo on spinal cord crude membrane preparation of intact animals. We observed significant increase in ATP, ADP and AMP hydrolyzing activity in the spinal cord crude membrane preparation in EAE animals at 15 days post immunization compared to control animals. The increase was registered at 28 days post immunization, as well. At same time points, ribavirin treatment decreased ATP, ADP and AMP hydrolyzing activity compared to EAE animals. In addition, no significant changes 8 days post immunization was observed between EAE-induced and ribavirin- treated EAE animals and these levels were similar to control level. Thus, we suppose that ribavirin-induced alteration in ectonucleotidase activities is rather due to its suppression of inflammation, than to its direct action on ATP, ADP and AMP hydrolysis.",
journal = "General Physiology and Biophysics",
title = "Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis",
number = "null",
volume = "28",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1474"
}

Lavrnja, I., Nedeljković, N. N., Bjelobaba, I., Savić, D., Dačić, S., Peković, S., Rakić, L., Mostarica-Stojković, M. B., Stošić-Grujičić, S.,& Stojiljković, M. B.. (2009). Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1474

Lavrnja I, Nedeljković NN, Bjelobaba I, Savić D, Dačić S, Peković S, Rakić L, Mostarica-Stojković MB, Stošić-Grujičić S, Stojiljković MB. Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis. in General Physiology and Biophysics. 2009;28(null):null-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1474 .

Lavrnja, Irena, Nedeljković, Nadezda N, Bjelobaba, Ivana, Savić, Danijela, Dačić, Sanja, Peković, Sanja, Rakić, Ljubisav, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Stojiljković, Mirjana B, "Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1474 .

TY  - JOUR
AU  - Perović, Milka
AU  - Mladenović, Aleksandra
AU  - Smiljanić, Kosara
AU  - Tanić, Nikola T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1420
AB  - Expression profiles of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46), proteins involved in cholesterol biosynthesis, transport and excretion from the CNS, were analyzed in the rat cortex, hippocampus and cerebellum as a function of aging (6-24 months) and in response to long-term dietary restriction (DR). Age-related increases for all three mRNAs were observed, with the highest induction found for Cyp46 in the cortex and hippocampus of 24-month-old animals. DR maintained stable levels of Cyp46, HMGR, and ApoE mRNAs during aging, exhibiting an attenuating effect on age-related changes through specific temporal and regional pattern. Neither age nor DR had any prominent effects at the protein level, except for Cyp46 and ApoE protein levels in the hippocampus and cerebellum, respectively. Overall, the changes in the cerebellum were different from those in the cortex and hippocampus. Our results demonstrated a modulatory effect of DR on age-related changes of CYP46, HMGR, and ApoE and suggest that the anti-aging effect of DR is in part mediated though transcriptional modulation of cholesterol metabolism genes in the rat brain.
T2  - Biogerontology
T1  - Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction
IS  - 6
VL  - 10
EP  - 745
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1420
ER  - 

@article{
author = "Perović, Milka and Mladenović, Aleksandra and Smiljanić, Kosara and Tanić, Nikola T and Rakić, Ljubisav and Ruždijić, Sabera and Kanazir, Selma",
year = "2009",
abstract = "Expression profiles of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46), proteins involved in cholesterol biosynthesis, transport and excretion from the CNS, were analyzed in the rat cortex, hippocampus and cerebellum as a function of aging (6-24 months) and in response to long-term dietary restriction (DR). Age-related increases for all three mRNAs were observed, with the highest induction found for Cyp46 in the cortex and hippocampus of 24-month-old animals. DR maintained stable levels of Cyp46, HMGR, and ApoE mRNAs during aging, exhibiting an attenuating effect on age-related changes through specific temporal and regional pattern. Neither age nor DR had any prominent effects at the protein level, except for Cyp46 and ApoE protein levels in the hippocampus and cerebellum, respectively. Overall, the changes in the cerebellum were different from those in the cortex and hippocampus. Our results demonstrated a modulatory effect of DR on age-related changes of CYP46, HMGR, and ApoE and suggest that the anti-aging effect of DR is in part mediated though transcriptional modulation of cholesterol metabolism genes in the rat brain.",
journal = "Biogerontology",
title = "Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction",
number = "6",
volume = "10",
pages = "745",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1420"
}

Perović, M., Mladenović, A., Smiljanić, K., Tanić, N. T., Rakić, L., Ruždijić, S.,& Kanazir, S.. (2009). Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction. in Biogerontology, 10(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1420

Perović M, Mladenović A, Smiljanić K, Tanić NT, Rakić L, Ruždijić S, Kanazir S. Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction. in Biogerontology. 2009;10(6):null-745.
https://hdl.handle.net/21.15107/rcub_ibiss_1420 .

Perović, Milka, Mladenović, Aleksandra, Smiljanić, Kosara, Tanić, Nikola T, Rakić, Ljubisav, Ruždijić, Sabera, Kanazir, Selma, "Expression of cholesterol homeostasis genes in the brain of the male rat is affected by age and dietary restriction" in Biogerontology, 10, no. 6 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1420 .

TY  - JOUR
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Jovanović, Saša
AU  - Nedeljković, Nadežda N.
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1534
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.
PB  - New York, USA: Springer - Plenum publishers
T2  - Journal of the Neurological Sciences
T1  - Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation
IS  - 1-2
VL  - 267
DO  - 10.1016/j.jns.2007.10.010
SP  - 76
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1534
ER  - 

@article{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Dacić, Sanja and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Jovanović, Saša and Nedeljković, Nadežda N. and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.",
publisher = "New York, USA: Springer - Plenum publishers",
journal = "Journal of the Neurological Sciences",
title = "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation",
number = "1-2",
volume = "267",
doi = "10.1016/j.jns.2007.10.010",
pages = "76-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1534"
}

Savić, D., Lavrnja, I., Peković, S., Dacić, S., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Jovanović, S., Nedeljković, N. N., Rakić, L.,& Stojiljković, M. B.. (2008). Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences
New York, USA: Springer - Plenum publishers., 267(1-2), 76-85.
https://doi.org/10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534

Savić D, Lavrnja I, Peković S, Dacić S, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Jovanović S, Nedeljković NN, Rakić L, Stojiljković MB. Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences. 2008;267(1-2):76-85.
doi:10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Dacić, Sanja, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Jovanović, Saša, Nedeljković, Nadežda N., Rakić, Ljubisav, Stojiljković, Mirjana B., "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation" in Journal of the Neurological Sciences, 267, no. 1-2 (2008):76-85,
https://doi.org/10.1016/j.jns.2007.10.010 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

TY  - JOUR
AU  - Nedeljković, Nadežda N.
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1533
AB  - During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Neurochemical Research
T1  - Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat
IS  - 5
VL  - 33
DO  - 10.1007/s11064-007-9529-0
SP  - 873
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1533
ER  - 

@article{
author = "Nedeljković, Nadežda N. and Bjelobaba, Ivana and Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Neurochemical Research",
title = "Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat",
number = "5",
volume = "33",
doi = "10.1007/s11064-007-9529-0",
pages = "873-879",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1533"
}

Nedeljković, N. N., Bjelobaba, I., Lavrnja, I., Savić, D., Peković, S., Rakić, L.,& Stojiljković, M. B.. (2008). Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat. in Neurochemical Research
Amsterdam, Netherlands: Elsevier., 33(5), 873-879.
https://doi.org/10.1007/s11064-007-9529-0
https://hdl.handle.net/21.15107/rcub_ibiss_1533

Nedeljković NN, Bjelobaba I, Lavrnja I, Savić D, Peković S, Rakić L, Stojiljković MB. Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat. in Neurochemical Research. 2008;33(5):873-879.
doi:10.1007/s11064-007-9529-0
https://hdl.handle.net/21.15107/rcub_ibiss_1533 .

Nedeljković, Nadežda N., Bjelobaba, Ivana, Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana B., "Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat" in Neurochemical Research, 33, no. 5 (2008):873-879,
https://doi.org/10.1007/s11064-007-9529-0 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1533 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Bjelobaba, Ivana
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Nedeljković, Nadežda N.
AU  - Mostarica-Stojković, Marija B.
AU  - Stošić-Grujičić, Stanislava
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1141
AB  - To determine the mechanism underlying ribavirin induced amelioration of experimental autoimmune encephalomyelitis (EAE), cytokine profiles were evaluated in draining lymph node (DLN) cell culture supernatants and spinal cord obtained from EAE and/or ribavirin-treated EAE Dark Agouti rats. Administration of ribavirin to EAE rats markedly affected the production of pro-inflammatory cytokines IFN-gamma, IL-1 beta and TNF-alpha in DLN and spinal cord, thus shifting the balance towards the anti-inflammatory cytokines IL-10 and TGF-beta. These findings suggest that ribavirin attenuates EAE by limiting cytokine-mediated immunoinflammatory events leading to CNS destruction. The conducted experiments provide rationale for ribavirin to be considered as a candidate drug in the development of new therapeutic strategies for the treatment of autoimmune diseases in humans, such as multiple sclerosis. (c) 2008 Elsevier B.V. All rights reserved.
PB  - Amsterdam, Netherlands: Elsevier
T2  - International Immunopharmacology
T1  - Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production
IS  - 9
VL  - 8
DO  - 10.1016/j.intimp.2008.05.008
SP  - 1282
EP  - 1290
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1141
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Bjelobaba, Ivana and Peković, Sanja and Dacić, Sanja and Nedeljković, Nadežda N. and Mostarica-Stojković, Marija B. and Stošić-Grujičić, Stanislava and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "To determine the mechanism underlying ribavirin induced amelioration of experimental autoimmune encephalomyelitis (EAE), cytokine profiles were evaluated in draining lymph node (DLN) cell culture supernatants and spinal cord obtained from EAE and/or ribavirin-treated EAE Dark Agouti rats. Administration of ribavirin to EAE rats markedly affected the production of pro-inflammatory cytokines IFN-gamma, IL-1 beta and TNF-alpha in DLN and spinal cord, thus shifting the balance towards the anti-inflammatory cytokines IL-10 and TGF-beta. These findings suggest that ribavirin attenuates EAE by limiting cytokine-mediated immunoinflammatory events leading to CNS destruction. The conducted experiments provide rationale for ribavirin to be considered as a candidate drug in the development of new therapeutic strategies for the treatment of autoimmune diseases in humans, such as multiple sclerosis. (c) 2008 Elsevier B.V. All rights reserved.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "International Immunopharmacology",
title = "Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production",
number = "9",
volume = "8",
doi = "10.1016/j.intimp.2008.05.008",
pages = "1282-1290",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1141"
}

Lavrnja, I., Savić, D., Bjelobaba, I., Peković, S., Dacić, S., Nedeljković, N. N., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Rakić, L.,& Stojiljković, M. B.. (2008). Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production. in International Immunopharmacology
Amsterdam, Netherlands: Elsevier., 8(9), 1282-1290.
https://doi.org/10.1016/j.intimp.2008.05.008
https://hdl.handle.net/21.15107/rcub_ibiss_1141

Lavrnja I, Savić D, Bjelobaba I, Peković S, Dacić S, Nedeljković NN, Mostarica-Stojković MB, Stošić-Grujičić S, Rakić L, Stojiljković MB. Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production. in International Immunopharmacology. 2008;8(9):1282-1290.
doi:10.1016/j.intimp.2008.05.008
https://hdl.handle.net/21.15107/rcub_ibiss_1141 .

Lavrnja, Irena, Savić, Danijela, Bjelobaba, Ivana, Peković, Sanja, Dacić, Sanja, Nedeljković, Nadežda N., Mostarica-Stojković, Marija B., Stošić-Grujičić, Stanislava, Rakić, Ljubisav, Stojiljković, Mirjana B., "Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production" in International Immunopharmacology, 8, no. 9 (2008):1282-1290,
https://doi.org/10.1016/j.intimp.2008.05.008 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1141 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadežda N.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1579
AB  - Distribution of two enzymes involved in the ectonucleotidase enzyme chain, ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5'-nucleotidase, was assessed by immunohistochemistry in the rat hippocampus. Obtained results have shown co-expression of the enzymes in the hippocampal region, as well as wide and strikingly similar cellular distribution. Both enzymes were expressed at the surface of pyramidal neurons in the CA1 and CA2 sections, while cells in the CA3 section were faintly stained. The granule cell layer of the dentate gyrus was moderately stained for NTPDase1, as well as for ecto-5'-nucleotidase. Glial association for ecto-5'-nucleotidase was also observed, and fiber tracts were intensively stained for both enzymes. This is the first comparative study of NTPDase1 and ecto-5'-nucleotidase distribution in the rat hippocampus. Obtained results suggest that the broad overlapping distribution of these enzymes in neurons and glial cells reflects the functional importance of ectonucleotidase actions in the nervous system.
PB  - New York, NY: Springer
T2  - Cellular and Molecular Neurobiology
T1  - Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution
IS  - 6
VL  - 27
DO  - 10.1007/s10571-007-9159-8
SP  - 731
EP  - 743
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1579
ER  - 

@article{
author = "Bjelobaba, Ivana and Stojiljković, Mirjana B and Peković, Sanja and Dacić, Sanja and Lavrnja, Irena and Savić, Danijela and Rakić, Ljubisav and Nedeljković, Nadežda N.",
year = "2007",
abstract = "Distribution of two enzymes involved in the ectonucleotidase enzyme chain, ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5'-nucleotidase, was assessed by immunohistochemistry in the rat hippocampus. Obtained results have shown co-expression of the enzymes in the hippocampal region, as well as wide and strikingly similar cellular distribution. Both enzymes were expressed at the surface of pyramidal neurons in the CA1 and CA2 sections, while cells in the CA3 section were faintly stained. The granule cell layer of the dentate gyrus was moderately stained for NTPDase1, as well as for ecto-5'-nucleotidase. Glial association for ecto-5'-nucleotidase was also observed, and fiber tracts were intensively stained for both enzymes. This is the first comparative study of NTPDase1 and ecto-5'-nucleotidase distribution in the rat hippocampus. Obtained results suggest that the broad overlapping distribution of these enzymes in neurons and glial cells reflects the functional importance of ectonucleotidase actions in the nervous system.",
publisher = "New York, NY: Springer",
journal = "Cellular and Molecular Neurobiology",
title = "Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution",
number = "6",
volume = "27",
doi = "10.1007/s10571-007-9159-8",
pages = "731-743",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1579"
}

Bjelobaba, I., Stojiljković, M. B., Peković, S., Dacić, S., Lavrnja, I., Savić, D., Rakić, L.,& Nedeljković, N. N.. (2007). Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution. in Cellular and Molecular Neurobiology
New York, NY: Springer., 27(6), 731-743.
https://doi.org/10.1007/s10571-007-9159-8
https://hdl.handle.net/21.15107/rcub_ibiss_1579

Bjelobaba I, Stojiljković MB, Peković S, Dacić S, Lavrnja I, Savić D, Rakić L, Nedeljković NN. Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution. in Cellular and Molecular Neurobiology. 2007;27(6):731-743.
doi:10.1007/s10571-007-9159-8
https://hdl.handle.net/21.15107/rcub_ibiss_1579 .

Bjelobaba, Ivana, Stojiljković, Mirjana B, Peković, Sanja, Dacić, Sanja, Lavrnja, Irena, Savić, Danijela, Rakić, Ljubisav, Nedeljković, Nadežda N., "Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution" in Cellular and Molecular Neurobiology, 27, no. 6 (2007):731-743,
https://doi.org/10.1007/s10571-007-9159-8 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1579 .

TY  - CONF
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Šubašić, Sanja A
AU  - Jovanović, Sasa
AU  - Nikić, Ivana
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1636
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.
C3  - 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan
T1  - Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis
SP  - 167
EP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1636
ER  - 

@conference{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Šubašić, Sanja A and Jovanović, Sasa and Nikić, Ivana and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.",
journal = "8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan",
title = "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis",
pages = "167-168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1636"
}

Savić, D., Lavrnja, I., Peković, S., Šubašić, S. A., Jovanović, S., Nikić, I., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Rakić, L.,& Stojiljković, M. B.. (2006). Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan, 167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636

Savić D, Lavrnja I, Peković S, Šubašić SA, Jovanović S, Nikić I, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Rakić L, Stojiljković MB. Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan. 2006;:167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Šubašić, Sanja A, Jovanović, Sasa, Nikić, Ivana, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Rakić, Ljubisav, Stojiljković, Mirjana B, "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis" in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan (2006):167-168,
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda N.
AU  - Šubašić, Sanja A
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1627
AB  - Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.
PB  - Elsevier
T2  - Brain Research
T1  - Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain
IS  - 1
VL  - 1120
DO  - 10.1016/j.brainres.2006.08.114
SP  - 54
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1627
ER  - 

@article{
author = "Bjelobaba, Ivana and Nedeljković, Nadežda N. and Šubašić, Sanja A and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.",
publisher = "Elsevier",
journal = "Brain Research",
title = "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain",
number = "1",
volume = "1120",
doi = "10.1016/j.brainres.2006.08.114",
pages = "54-63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1627"
}

Bjelobaba, I., Nedeljković, N. N., Šubašić, S. A., Lavrnja, I., Peković, S., Savić, D., Rakić, L.,& Stojiljković, M. B.. (2006). Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research
Elsevier., 1120(1), 54-63.
https://doi.org/10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627

Bjelobaba I, Nedeljković NN, Šubašić SA, Lavrnja I, Peković S, Savić D, Rakić L, Stojiljković MB. Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research. 2006;1120(1):54-63.
doi:10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

Bjelobaba, Ivana, Nedeljković, Nadežda N., Šubašić, Sanja A, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Rakić, Ljubisav, Stojiljković, Mirjana B, "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain" in Brain Research, 1120, no. 1 (2006):54-63,
https://doi.org/10.1016/j.brainres.2006.08.114 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

TY  - JOUR
AU  - Nedeljković, Nadežda
AU  - Bjelobaba, Ivana
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Vještica, Aleksandar
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2006
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2006.03.001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3464
UR  - https://www.sciencedirect.com/science/article/abs/pii/S1065699506000643
AB  - The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.
PB  - Elsevier
T2  - Cell Biology International
T1  - Up-regulation of ectonucleotidase activity after cortical stab injury in rats
IS  - 6
VL  - 30
DO  - 10.1016/j.cellbi.2006.03.001
SP  - 541
EP  - 546
ER  - 

@article{
author = "Nedeljković, Nadežda and Bjelobaba, Ivana and Dacić, Sanja and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Vještica, Aleksandar and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2006",
abstract = "The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.",
publisher = "Elsevier",
journal = "Cell Biology International",
title = "Up-regulation of ectonucleotidase activity after cortical stab injury in rats",
number = "6",
volume = "30",
doi = "10.1016/j.cellbi.2006.03.001",
pages = "541-546"
}

Nedeljković, N., Bjelobaba, I., Dacić, S., Lavrnja, I., Peković, S., Savić, D., Vještica, A., Rakić, L.,& Stojiljković, M.. (2006). Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International
Elsevier., 30(6), 541-546.
https://doi.org/10.1016/j.cellbi.2006.03.001

Nedeljković N, Bjelobaba I, Dacić S, Lavrnja I, Peković S, Savić D, Vještica A, Rakić L, Stojiljković M. Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International. 2006;30(6):541-546.
doi:10.1016/j.cellbi.2006.03.001 .

Nedeljković, Nadežda, Bjelobaba, Ivana, Dacić, Sanja, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Vještica, Aleksandar, Rakić, Ljubisav, Stojiljković, Mirjana, "Up-regulation of ectonucleotidase activity after cortical stab injury in rats" in Cell Biology International, 30, no. 6 (2006):541-546,
https://doi.org/10.1016/j.cellbi.2006.03.001 . .

TY  - JOUR
AU  - Peković, Sanja
AU  - Filipović, Radmila
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Nedeljković, Nadežda
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2005
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5870
AB  - The weak regenerative capacity of self-repair after injury to the adult brain is caused by the formation of glial sear due to reactive astrogliosis. In the present study the beginning of reactive astrogliosis in the adult, as shown immunocytochemically by upregulation of glial fibrillary acidic protein (GFAP) and vimentin, was seen two days after the left sensorimotor cortex lesion, being maximal during the first two weeks and declining by 30 days after the lesion. This was accompanied by intensive glial scarring. Conversely, after the neonatal lesion a lack of gliotic scar was seen until 30 days postsurgery, although the pattern of GFAP and vimentin expression during recovery period was the same. The aim of the study was to define an appropriate therapeutic intervention that could modulate astrocyte proliferation and diminish glial scar formation after adult brain lesion. For this purpose the effects of an antiproliferative agent, the purine nucleoside analogue ribavirin was examined. It was shown that daily injection of ribavirin for 5 and 10 days considerably decreased the number of reactive astrocytes, while slight GFAP labeling was restricted to the lesion site. Obtained results show that ribavirin treatment downregulates the process of reactive astrogliosis after adult brain injury, and thus may be a useful approach for improving neurological recovery from brain damage.
PB  - Hoboken, NJ: Wiley
T2  - Annals of the New York Academy of Sciences
T1  - Downregulation of Glial Scarring after Brain Injury: The Effect of Purine Nucleoside Analogue Ribavirin
IS  - 1
VL  - 1048
DO  - 10.1196/annals.1342.027
SP  - 296
EP  - 310
ER  - 

@article{
author = "Peković, Sanja and Filipović, Radmila and Dacić, Sanja and Lavrnja, Irena and Savić, Danijela and Nedeljković, Nadežda and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2005",
abstract = "The weak regenerative capacity of self-repair after injury to the adult brain is caused by the formation of glial sear due to reactive astrogliosis. In the present study the beginning of reactive astrogliosis in the adult, as shown immunocytochemically by upregulation of glial fibrillary acidic protein (GFAP) and vimentin, was seen two days after the left sensorimotor cortex lesion, being maximal during the first two weeks and declining by 30 days after the lesion. This was accompanied by intensive glial scarring. Conversely, after the neonatal lesion a lack of gliotic scar was seen until 30 days postsurgery, although the pattern of GFAP and vimentin expression during recovery period was the same. The aim of the study was to define an appropriate therapeutic intervention that could modulate astrocyte proliferation and diminish glial scar formation after adult brain lesion. For this purpose the effects of an antiproliferative agent, the purine nucleoside analogue ribavirin was examined. It was shown that daily injection of ribavirin for 5 and 10 days considerably decreased the number of reactive astrocytes, while slight GFAP labeling was restricted to the lesion site. Obtained results show that ribavirin treatment downregulates the process of reactive astrogliosis after adult brain injury, and thus may be a useful approach for improving neurological recovery from brain damage.",
publisher = "Hoboken, NJ: Wiley",
journal = "Annals of the New York Academy of Sciences",
title = "Downregulation of Glial Scarring after Brain Injury: The Effect of Purine Nucleoside Analogue Ribavirin",
number = "1",
volume = "1048",
doi = "10.1196/annals.1342.027",
pages = "296-310"
}

Peković, S., Filipović, R., Dacić, S., Lavrnja, I., Savić, D., Nedeljković, N., Rakić, L.,& Stojiljković, M.. (2005). Downregulation of Glial Scarring after Brain Injury: The Effect of Purine Nucleoside Analogue Ribavirin. in Annals of the New York Academy of Sciences
Hoboken, NJ: Wiley., 1048(1), 296-310.
https://doi.org/10.1196/annals.1342.027

Peković S, Filipović R, Dacić S, Lavrnja I, Savić D, Nedeljković N, Rakić L, Stojiljković M. Downregulation of Glial Scarring after Brain Injury: The Effect of Purine Nucleoside Analogue Ribavirin. in Annals of the New York Academy of Sciences. 2005;1048(1):296-310.
doi:10.1196/annals.1342.027 .

Peković, Sanja, Filipović, Radmila, Dacić, Sanja, Lavrnja, Irena, Savić, Danijela, Nedeljković, Nadežda, Rakić, Ljubisav, Stojiljković, Mirjana, "Downregulation of Glial Scarring after Brain Injury: The Effect of Purine Nucleoside Analogue Ribavirin" in Annals of the New York Academy of Sciences, 1048, no. 1 (2005):296-310,
https://doi.org/10.1196/annals.1342.027 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Pešić, Vesna
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2005
UR  - https://link.springer.com/article/10.1007%2Fs00221-005-2311-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3809
AB  - The time-course of changes of basal and amphetamine (AMPH)-induced locomotor and stereotypic activities in adult male Wistar rats after a single ribavirin injection was studied. In the first set of experiments, 10, 20 or 30 mg ribavirin/kg body weight (b.w.) were injected i.p. to rats and their basal motor activities were recorded every 10 min for 2 h and compared with those of saline-treated controls. In the second set of experiments, the animals were pretreated with ribavirin and 20 min later i.p. injected with AMPH (1.5 mg/kg b.w.). The controls received AMPH 20 min after the saline injection. Motor activity was recorded after the first injection and until 120 min after AMPH administration. Ribavirin did not significantly affect the time-course of either basal locomotor or stereotypic activities. Pretreatment with any of the applied ribavirin doses decreased the AMPH-induced hyperlocomotor response. However, the most pronounced effect was observed with ribavirin doses of 20 mg/kg and 30 mg/kg when administered during the first 10 min and 30 min after the AMPH injection respectively. In contrast, the stereotypic activities of these animals were only slightly changed. These results indicate a different susceptibility of regions in the basal ganglia to ribavirin.
PB  - Springer
T2  - Experimental Brain Research
T1  - The time-course of ribavirin-provoked changes of basal and AMPH-induced motor activities in rats
IS  - 3
VL  - 165
DO  - 10.1007/s00221-005-2311-0
SP  - 402
EP  - 406
ER  - 

@article{
author = "Petković, Branka and Pešić, Vesna and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2005",
abstract = "The time-course of changes of basal and amphetamine (AMPH)-induced locomotor and stereotypic activities in adult male Wistar rats after a single ribavirin injection was studied. In the first set of experiments, 10, 20 or 30 mg ribavirin/kg body weight (b.w.) were injected i.p. to rats and their basal motor activities were recorded every 10 min for 2 h and compared with those of saline-treated controls. In the second set of experiments, the animals were pretreated with ribavirin and 20 min later i.p. injected with AMPH (1.5 mg/kg b.w.). The controls received AMPH 20 min after the saline injection. Motor activity was recorded after the first injection and until 120 min after AMPH administration. Ribavirin did not significantly affect the time-course of either basal locomotor or stereotypic activities. Pretreatment with any of the applied ribavirin doses decreased the AMPH-induced hyperlocomotor response. However, the most pronounced effect was observed with ribavirin doses of 20 mg/kg and 30 mg/kg when administered during the first 10 min and 30 min after the AMPH injection respectively. In contrast, the stereotypic activities of these animals were only slightly changed. These results indicate a different susceptibility of regions in the basal ganglia to ribavirin.",
publisher = "Springer",
journal = "Experimental Brain Research",
title = "The time-course of ribavirin-provoked changes of basal and AMPH-induced motor activities in rats",
number = "3",
volume = "165",
doi = "10.1007/s00221-005-2311-0",
pages = "402-406"
}

Petković, B., Pešić, V., Peković, S., Rakić, L.,& Stojiljković, M.. (2005). The time-course of ribavirin-provoked changes of basal and AMPH-induced motor activities in rats. in Experimental Brain Research
Springer., 165(3), 402-406.
https://doi.org/10.1007/s00221-005-2311-0

Petković B, Pešić V, Peković S, Rakić L, Stojiljković M. The time-course of ribavirin-provoked changes of basal and AMPH-induced motor activities in rats. in Experimental Brain Research. 2005;165(3):402-406.
doi:10.1007/s00221-005-2311-0 .

Petković, Branka, Pešić, Vesna, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana, "The time-course of ribavirin-provoked changes of basal and AMPH-induced motor activities in rats" in Experimental Brain Research, 165, no. 3 (2005):402-406,
https://doi.org/10.1007/s00221-005-2311-0 . .

TY  - JOUR
AU  - Milenković, Ivanka M.
AU  - Filipović, R
AU  - Nedeljković, Nadezda N
AU  - Peković, Sanja
AU  - Ćulić, Milka
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1731
AB  - 1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2 - 30 days following unilateral injection of kainic acid (KA) or physiological saline (SC). 2. Analysis of the staining intensity and pattern demonstrated that injection of both KA and physiological saline elicited significant and long-lasting increase of pNFP and npNFP immunoreactivity, at the ipsilateral, and to lesser extent at the contralateral side of lesion. 3. Kainate intoxication induced abundant expression of pNFP and npNFP in cerebellar white matter, as well as in all layers of perilesioned cortex. Higher pNFP expression was evidenced in the Purkinje cell layer, particularly at cell bodies, initial segments, and proximal dendrites, which normally do not contain pNFP. In addition, synaptophysin immunocytochemistry was used as a marker of synaptogenesis and plasticity. 4. Spatio-temporal pattern of NFP and synaptophysin expression suggests that perilesioned cortex undergoes dynamic changes following brain demage and possess a reparative capacity to abridge the consequences of brain trauma.
T2  - Cellular and Molecular Neurobiology
T1  - Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats
IS  - 3
VL  - 24
EP  - 378
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1731
ER  - 

@article{
author = "Milenković, Ivanka M. and Filipović, R and Nedeljković, Nadezda N and Peković, Sanja and Ćulić, Milka and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2004",
abstract = "1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2 - 30 days following unilateral injection of kainic acid (KA) or physiological saline (SC). 2. Analysis of the staining intensity and pattern demonstrated that injection of both KA and physiological saline elicited significant and long-lasting increase of pNFP and npNFP immunoreactivity, at the ipsilateral, and to lesser extent at the contralateral side of lesion. 3. Kainate intoxication induced abundant expression of pNFP and npNFP in cerebellar white matter, as well as in all layers of perilesioned cortex. Higher pNFP expression was evidenced in the Purkinje cell layer, particularly at cell bodies, initial segments, and proximal dendrites, which normally do not contain pNFP. In addition, synaptophysin immunocytochemistry was used as a marker of synaptogenesis and plasticity. 4. Spatio-temporal pattern of NFP and synaptophysin expression suggests that perilesioned cortex undergoes dynamic changes following brain demage and possess a reparative capacity to abridge the consequences of brain trauma.",
journal = "Cellular and Molecular Neurobiology",
title = "Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats",
number = "3",
volume = "24",
pages = "378",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1731"
}

Milenković, I. M., Filipović, R., Nedeljković, N. N., Peković, S., Ćulić, M., Rakić, L.,& Stojiljković, M. B.. (2004). Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats. in Cellular and Molecular Neurobiology, 24(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1731

Milenković IM, Filipović R, Nedeljković NN, Peković S, Ćulić M, Rakić L, Stojiljković MB. Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats. in Cellular and Molecular Neurobiology. 2004;24(3):null-378.
https://hdl.handle.net/21.15107/rcub_ibiss_1731 .

Milenković, Ivanka M., Filipović, R, Nedeljković, Nadezda N, Peković, Sanja, Ćulić, Milka, Rakić, Ljubisav, Stojiljković, Mirjana B, "Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats" in Cellular and Molecular Neurobiology, 24, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1731 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Subasić, Sanja A
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Medić-Mijačević, Ljubica
AU  - Pejanović, V
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1768
AB  - The effect of ribavirin on development of experimental autoimmune encephalomyelitis (EAE) was investigated. The disease was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant (SCH-CFA). Depending on the amount of mycobacteria in CFA, the animals developed either moderate or severe EAE. Ribavirin (1-beta-Dribofuranosyl-1,2,4-triazole-3-carboxamide) was applied i.p. at a daily dosage of 30 mg/kg in two treatment protocols: from the start of immunization (preventive treatment) or from the onset of the first EAE signs after the induction (therapeutic treatment). Signs of EAE began between 7 and 9 days after induction and peaked at days 11-13. In moderate EAE (mean maximal severity score 3.33 +/- 0.21), the recovery was completed by days 23-26, whereas, in severe EAE (mean maximal severity score 4.5 +/- 0.23), obvious recovery was not detected. Preventive ribavirin treatment significantly decreased clinical signs after both moderate (score 1.75 +/- 0.25, P < 0.05) and severe (score 3.62 +/- 0.31, P < 0.015) immunization. Also, disease manifestations were reduced by therapeutic treatment of ribavirin (mean maximal severity score 2.5 +/- 0.2 vs. 3.33 +/- 0.21 in controls, P < 0.005) but less so in comparison with preventive treatment. Analysis of the effects of ribavirin on histopathologic changes in the spinal cord tissue revealed a reduction of mononuclear cell infiltrates, composed of T cells and macrophages/microglia, and the absence of demyelination, which were pronounced in control EAE animals. Beneficial effects of preventive and therapeutic treatment with ribavirin on development of EAE suggest this nucleoside analogue as a useful candidate for therapy in multiple sclerosis. (C) 2003 Wiley-Liss, Inc.
T2  - Journal of Neuroscience Research
T1  - Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats
IS  - 2
VL  - 72
EP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1768
ER  - 

@article{
author = "Lavrnja, Irena and Peković, Sanja and Subasić, Sanja A and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Medić-Mijačević, Ljubica and Pejanović, V and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2003",
abstract = "The effect of ribavirin on development of experimental autoimmune encephalomyelitis (EAE) was investigated. The disease was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant (SCH-CFA). Depending on the amount of mycobacteria in CFA, the animals developed either moderate or severe EAE. Ribavirin (1-beta-Dribofuranosyl-1,2,4-triazole-3-carboxamide) was applied i.p. at a daily dosage of 30 mg/kg in two treatment protocols: from the start of immunization (preventive treatment) or from the onset of the first EAE signs after the induction (therapeutic treatment). Signs of EAE began between 7 and 9 days after induction and peaked at days 11-13. In moderate EAE (mean maximal severity score 3.33 +/- 0.21), the recovery was completed by days 23-26, whereas, in severe EAE (mean maximal severity score 4.5 +/- 0.23), obvious recovery was not detected. Preventive ribavirin treatment significantly decreased clinical signs after both moderate (score 1.75 +/- 0.25, P < 0.05) and severe (score 3.62 +/- 0.31, P < 0.015) immunization. Also, disease manifestations were reduced by therapeutic treatment of ribavirin (mean maximal severity score 2.5 +/- 0.2 vs. 3.33 +/- 0.21 in controls, P < 0.005) but less so in comparison with preventive treatment. Analysis of the effects of ribavirin on histopathologic changes in the spinal cord tissue revealed a reduction of mononuclear cell infiltrates, composed of T cells and macrophages/microglia, and the absence of demyelination, which were pronounced in control EAE animals. Beneficial effects of preventive and therapeutic treatment with ribavirin on development of EAE suggest this nucleoside analogue as a useful candidate for therapy in multiple sclerosis. (C) 2003 Wiley-Liss, Inc.",
journal = "Journal of Neuroscience Research",
title = "Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats",
number = "2",
volume = "72",
pages = "278",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1768"
}

Lavrnja, I., Peković, S., Subasić, S. A., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Medić-Mijačević, L., Pejanović, V., Rakić, L.,& Stojiljković, M. B.. (2003). Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats. in Journal of Neuroscience Research, 72(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1768

Lavrnja I, Peković S, Subasić SA, Mostarica-Stojković MB, Stošić-Grujičić S, Medić-Mijačević L, Pejanović V, Rakić L, Stojiljković MB. Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats. in Journal of Neuroscience Research. 2003;72(2):null-278.
https://hdl.handle.net/21.15107/rcub_ibiss_1768 .

Lavrnja, Irena, Peković, Sanja, Subasić, Sanja A, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Medić-Mijačević, Ljubica, Pejanović, V, Rakić, Ljubisav, Stojiljković, Mirjana B, "Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats" in Journal of Neuroscience Research, 72, no. 2 (2003),
https://hdl.handle.net/21.15107/rcub_ibiss_1768 .

TY  - JOUR
AU  - Pešić, Vesna
AU  - Veskov, Rosica
AU  - Petković, Branka
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2003
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0197458002002324?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3812
AB  - Changes in locomotor and stereotypic activities induced by an i.p. injection of either (+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)-cycloheptan-5,10- imine maleate (dizocilpine or MK-801; 0.3 mg/kg) or D-amphetamine sulfate (AMPH; 1.5 mg/kg) were studied in male Mill Hill hooded rats of different age. The following age groups of animals were considered: 28-30 postnatal day (PND)-old rats (peripubertal), 48-50 PND-old (pubertal), 3-month-old (adults), 12-month-old (middle-aged) and 24-month-old (aged). The motor response was measured by an automated animal activity measuring system. The obtained results showed that: (1) in contrast to AMPH, MK-801 induced more pronounced increases of both locomotor and stereotypic activities in peripubertal and pubertal than in adult and aged rats; (2) AMPH induced the same locomotor and stereotypic activity increase in pubertal, adult and middle-aged rats; (3) both AMPH and MK-801 led to a senescence-related decrease of motor activity. These data suggest that the balance of the glutamatergic and dopaminergic systems changes during aging. Such a change is important in understanding schizophrenia and the motor system decline observed in the later stages of life.
PB  - Elsevier Science Inc
T2  - Neurobiology of Aging
T1  - Age-related differences in MK-801- and amphetamine-induced locomotor and stereotypic activities of rats
IS  - 5
VL  - 24
DO  - 10.1016/S0197-4580(02)00232-4
SP  - 715
EP  - 723
ER  - 

@article{
author = "Pešić, Vesna and Veskov, Rosica and Petković, Branka and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2003",
abstract = "Changes in locomotor and stereotypic activities induced by an i.p. injection of either (+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)-cycloheptan-5,10- imine maleate (dizocilpine or MK-801; 0.3 mg/kg) or D-amphetamine sulfate (AMPH; 1.5 mg/kg) were studied in male Mill Hill hooded rats of different age. The following age groups of animals were considered: 28-30 postnatal day (PND)-old rats (peripubertal), 48-50 PND-old (pubertal), 3-month-old (adults), 12-month-old (middle-aged) and 24-month-old (aged). The motor response was measured by an automated animal activity measuring system. The obtained results showed that: (1) in contrast to AMPH, MK-801 induced more pronounced increases of both locomotor and stereotypic activities in peripubertal and pubertal than in adult and aged rats; (2) AMPH induced the same locomotor and stereotypic activity increase in pubertal, adult and middle-aged rats; (3) both AMPH and MK-801 led to a senescence-related decrease of motor activity. These data suggest that the balance of the glutamatergic and dopaminergic systems changes during aging. Such a change is important in understanding schizophrenia and the motor system decline observed in the later stages of life.",
publisher = "Elsevier Science Inc",
journal = "Neurobiology of Aging",
title = "Age-related differences in MK-801- and amphetamine-induced locomotor and stereotypic activities of rats",
number = "5",
volume = "24",
doi = "10.1016/S0197-4580(02)00232-4",
pages = "715-723"
}

Pešić, V., Veskov, R., Petković, B., Rakić, L.,& Stojiljković, M.. (2003). Age-related differences in MK-801- and amphetamine-induced locomotor and stereotypic activities of rats. in Neurobiology of Aging
Elsevier Science Inc., 24(5), 715-723.
https://doi.org/10.1016/S0197-4580(02)00232-4

Pešić V, Veskov R, Petković B, Rakić L, Stojiljković M. Age-related differences in MK-801- and amphetamine-induced locomotor and stereotypic activities of rats. in Neurobiology of Aging. 2003;24(5):715-723.
doi:10.1016/S0197-4580(02)00232-4 .

Pešić, Vesna, Veskov, Rosica, Petković, Branka, Rakić, Ljubisav, Stojiljković, Mirjana, "Age-related differences in MK-801- and amphetamine-induced locomotor and stereotypic activities of rats" in Neurobiology of Aging, 24, no. 5 (2003):715-723,
https://doi.org/10.1016/S0197-4580(02)00232-4 . .

TY  - JOUR
AU  - Pešić, Vesna
AU  - Petković, Branka
AU  - Milanović, Desanka
AU  - Tomić, Mirko
AU  - Veskov, Rosica
AU  - Rakić, Ljubisav
PY  - 2003
UR  - https://www.sciencedirect.com/science/article/pii/S0166432803001426?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3811
AB  - The aim of the present study was to examine motor effects of amphetamine (AMPH) in rats pretreated with either dizocilpine (MK-801) or phencyclidine (PCP), and to estimate possible differences in these effects. Our results showed that AMPH increases the duration of motor effects of PCP, while it does not change motor effects of MK-801. These findings may reflect different mechanisms of action of MK-801 and PCP, as well as selective influence of AMPH on metabolism of these drugs.
PB  - Elsevier
T2  - Behavioural Brain Research
T1  - Motor effects of amphetamine in rats pretreated with either dizocilpine or phencyclidine
IS  - 1-2
VL  - 147
DO  - 10.1016/S0166-4328(03)00142-6
SP  - 211
EP  - 215
ER  - 

@article{
author = "Pešić, Vesna and Petković, Branka and Milanović, Desanka and Tomić, Mirko and Veskov, Rosica and Rakić, Ljubisav",
year = "2003",
abstract = "The aim of the present study was to examine motor effects of amphetamine (AMPH) in rats pretreated with either dizocilpine (MK-801) or phencyclidine (PCP), and to estimate possible differences in these effects. Our results showed that AMPH increases the duration of motor effects of PCP, while it does not change motor effects of MK-801. These findings may reflect different mechanisms of action of MK-801 and PCP, as well as selective influence of AMPH on metabolism of these drugs.",
publisher = "Elsevier",
journal = "Behavioural Brain Research",
title = "Motor effects of amphetamine in rats pretreated with either dizocilpine or phencyclidine",
number = "1-2",
volume = "147",
doi = "10.1016/S0166-4328(03)00142-6",
pages = "211-215"
}

Pešić, V., Petković, B., Milanović, D., Tomić, M., Veskov, R.,& Rakić, L.. (2003). Motor effects of amphetamine in rats pretreated with either dizocilpine or phencyclidine. in Behavioural Brain Research
Elsevier., 147(1-2), 211-215.
https://doi.org/10.1016/S0166-4328(03)00142-6

Pešić V, Petković B, Milanović D, Tomić M, Veskov R, Rakić L. Motor effects of amphetamine in rats pretreated with either dizocilpine or phencyclidine. in Behavioural Brain Research. 2003;147(1-2):211-215.
doi:10.1016/S0166-4328(03)00142-6 .

Pešić, Vesna, Petković, Branka, Milanović, Desanka, Tomić, Mirko, Veskov, Rosica, Rakić, Ljubisav, "Motor effects of amphetamine in rats pretreated with either dizocilpine or phencyclidine" in Behavioural Brain Research, 147, no. 1-2 (2003):211-215,
https://doi.org/10.1016/S0166-4328(03)00142-6 . .

TY  - JOUR
AU  - Popov, Ivan
AU  - Veskov, Rosica
AU  - Spužić, Ivan
AU  - Rakić, Ljubisav
PY  - 2002
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/372
T2  - Archive of Oncology
T1  - Bilateral lesions of the caudate nuclei and effects of the psychotonic drug piracetam on cancer development in methylcholanthrene induced tumors in the rat
IS  - 3
VL  - 10
SP  - 224
EP  - 224
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_372
ER  - 

@article{
author = "Popov, Ivan and Veskov, Rosica and Spužić, Ivan and Rakić, Ljubisav",
year = "2002, 2002",
journal = "Archive of Oncology",
title = "Bilateral lesions of the caudate nuclei and effects of the psychotonic drug piracetam on cancer development in methylcholanthrene induced tumors in the rat",
number = "3",
volume = "10",
pages = "224-224",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_372"
}

Popov, I., Veskov, R., Spužić, I.,& Rakić, L.. (2002). Bilateral lesions of the caudate nuclei and effects of the psychotonic drug piracetam on cancer development in methylcholanthrene induced tumors in the rat. in Archive of Oncology, 10(3), 224-224.
https://hdl.handle.net/21.15107/rcub_ibiss_372

Popov I, Veskov R, Spužić I, Rakić L. Bilateral lesions of the caudate nuclei and effects of the psychotonic drug piracetam on cancer development in methylcholanthrene induced tumors in the rat. in Archive of Oncology. 2002;10(3):224-224.
https://hdl.handle.net/21.15107/rcub_ibiss_372 .

Popov, Ivan, Veskov, Rosica, Spužić, Ivan, Rakić, Ljubisav, "Bilateral lesions of the caudate nuclei and effects of the psychotonic drug piracetam on cancer development in methylcholanthrene induced tumors in the rat" in Archive of Oncology, 10, no. 3 (2002):224-224,
https://hdl.handle.net/21.15107/rcub_ibiss_372 .