TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - CONF
AU  - Kilibarda, Sofija
AU  - Vuković, Sandra
AU  - Milinčić, Danijel
AU  - Mačukanović-Jocić, Marina
AU  - Jarić, Snežana
AU  - Kostić, Aleksandar
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4919
AB  - Athamanta turbith (L.) Brot. is the endemic flowering plant from the Apiaceae family
originated from Đetinja Canyon (Serbia). The aim of this study was to determine the content of
selected plant bioactive compounds present in rhizome, vegetative shoot and inflorescence. Extraction
was performed in 80% methanol as a solvent with two different approaches: powdered
plant material was extracted with solvent for 3 h in ratio 1:10 without (classical solvent extraction,
SE,) and with application of ultrasound (ultrasound-assisted extraction, UAE). Analysis of total
phenolic content (TPC), total flavonoid content (TFC) and total hydroxycinnamic acid derivative
content (HCA) was performed via spectrophotometric methods. The inflorescence had the highest
TPC in UAE obtained-extract (2.73 ± 0.13 mg GAE/g), as well as the highest TFC (1.56 ± 0.02 mg/g
QE) and HCA (1.45 ± 0.11 mg/g CGAE) in SE-prepared extract where GAE, QE and CGAE are gallic
acid equivalents, quercetin equivalents and chlorogenic acid equivalents respectively. The lowest
amount of TPC, TFC and HCA was detected in the rhizome regardless of extraction methods. Additionally,
antioxidant properties of extracts were determined with five assays: ABTS∙+, DPPH∙,
ferric reducing power (FRP), in vitro phosphomolybdenum total antioxidant capacity (TAC) and
cupric reducing antioxidant capacity (CUPRAC). The inflorescence had the highest antioxidant
activity in both quencher assays (at γ = 0.1 g/mL) with 92.1% of inhibition for ABTS∙+ (UAE extract,)
and 77.7% inhibition of DPPH∙ (for both extracts). In addition, it exhibited the highest FRP (18.4
mg/g AAE, SE extract and CUPRAC (~40 mg/g AAE for both extracts) values where AAE stands
for ascorbic acid equivalents. Whereas, the rhizome had the lowest values for all antioxidant assays
concerning both SE and UEA. In conclusion, exhibited antioxidant properties are mostly in line
with the determined content of selected bioactive compounds. Further statistical analysis is applied
to confirm/oppose this.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
T1  - Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia
DO  - 10.3390/IECPS2021-11947
SP  - 11947
ER  - 

@conference{
author = "Kilibarda, Sofija and Vuković, Sandra and Milinčić, Danijel and Mačukanović-Jocić, Marina and Jarić, Snežana and Kostić, Aleksandar",
year = "2021",
abstract = "Athamanta turbith (L.) Brot. is the endemic flowering plant from the Apiaceae family
originated from Đetinja Canyon (Serbia). The aim of this study was to determine the content of
selected plant bioactive compounds present in rhizome, vegetative shoot and inflorescence. Extraction
was performed in 80% methanol as a solvent with two different approaches: powdered
plant material was extracted with solvent for 3 h in ratio 1:10 without (classical solvent extraction,
SE,) and with application of ultrasound (ultrasound-assisted extraction, UAE). Analysis of total
phenolic content (TPC), total flavonoid content (TFC) and total hydroxycinnamic acid derivative
content (HCA) was performed via spectrophotometric methods. The inflorescence had the highest
TPC in UAE obtained-extract (2.73 ± 0.13 mg GAE/g), as well as the highest TFC (1.56 ± 0.02 mg/g
QE) and HCA (1.45 ± 0.11 mg/g CGAE) in SE-prepared extract where GAE, QE and CGAE are gallic
acid equivalents, quercetin equivalents and chlorogenic acid equivalents respectively. The lowest
amount of TPC, TFC and HCA was detected in the rhizome regardless of extraction methods. Additionally,
antioxidant properties of extracts were determined with five assays: ABTS∙+, DPPH∙,
ferric reducing power (FRP), in vitro phosphomolybdenum total antioxidant capacity (TAC) and
cupric reducing antioxidant capacity (CUPRAC). The inflorescence had the highest antioxidant
activity in both quencher assays (at γ = 0.1 g/mL) with 92.1% of inhibition for ABTS∙+ (UAE extract,)
and 77.7% inhibition of DPPH∙ (for both extracts). In addition, it exhibited the highest FRP (18.4
mg/g AAE, SE extract and CUPRAC (~40 mg/g AAE for both extracts) values where AAE stands
for ascorbic acid equivalents. Whereas, the rhizome had the lowest values for all antioxidant assays
concerning both SE and UEA. In conclusion, exhibited antioxidant properties are mostly in line
with the determined content of selected bioactive compounds. Further statistical analysis is applied
to confirm/oppose this.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online",
title = "Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia",
doi = "10.3390/IECPS2021-11947",
pages = "11947"
}

Kilibarda, S., Vuković, S., Milinčić, D., Mačukanović-Jocić, M., Jarić, S.,& Kostić, A.. (2021). Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
Basel: MDPI., 11947.
https://doi.org/10.3390/IECPS2021-11947

Kilibarda S, Vuković S, Milinčić D, Mačukanović-Jocić M, Jarić S, Kostić A. Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online. 2021;:11947.
doi:10.3390/IECPS2021-11947 .

Kilibarda, Sofija, Vuković, Sandra, Milinčić, Danijel, Mačukanović-Jocić, Marina, Jarić, Snežana, Kostić, Aleksandar, "Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia" in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online (2021):11947,
https://doi.org/10.3390/IECPS2021-11947 . .

TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Stanisavljević, Nemanja S.
AU  - Kostić, Aleksandar
AU  - Soković Bajić, Svetlana
AU  - Kojić, Milan O.
AU  - Gašić, Uroš
AU  - Barać, Miroljub B.
AU  - Stanojević, Sladjana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4092
AB  - Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS•+ DPPH• and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.
PB  - Academic Press
T2  - LWT
T2  - LWT
T1  - Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety
VL  - 138
DO  - 10.1016/j.lwt.2020.110739
SP  - 110739
ER  - 

@article{
author = "Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Kostić, Aleksandar and Soković Bajić, Svetlana and Kojić, Milan O. and Gašić, Uroš and Barać, Miroljub B. and Stanojević, Sladjana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS•+ DPPH• and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.",
publisher = "Academic Press",
journal = "LWT, LWT",
title = "Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety",
volume = "138",
doi = "10.1016/j.lwt.2020.110739",
pages = "110739"
}

Milinčić, D. D., Stanisavljević, N. S., Kostić, A., Soković Bajić, S., Kojić, M. O., Gašić, U., Barać, M. B., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety. in LWT
Academic Press., 138, 110739.
https://doi.org/10.1016/j.lwt.2020.110739

Milinčić DD, Stanisavljević NS, Kostić A, Soković Bajić S, Kojić MO, Gašić U, Barać MB, Stanojević SP, Tešić ŽL, Pešić MB. Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety. in LWT. 2021;138:110739.
doi:10.1016/j.lwt.2020.110739 .

Milinčić, Danijel D., Stanisavljević, Nemanja S., Kostić, Aleksandar, Soković Bajić, Svetlana, Kojić, Milan O., Gašić, Uroš, Barać, Miroljub B., Stanojević, Sladjana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety" in LWT, 138 (2021):110739,
https://doi.org/10.1016/j.lwt.2020.110739 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4315
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen
IS  - 7
VL  - 10
DO  - 10.3390/antiox10071091
SP  - 1091
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Nedić, Nebojša and Gašić, Uroš and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav and Pešić, Mirjana",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen",
number = "7",
volume = "10",
doi = "10.3390/antiox10071091",
pages = "1091"
}

Kostić, A., Milinčić, D., Nedić, N., Gašić, U., Špirović Trifunović, B., Vojt, D., Tešić, Ž.,& Pešić, M.. (2021). Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants
Basel: MDPI., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić A, Milinčić D, Nedić N, Gašić U, Špirović Trifunović B, Vojt D, Tešić Ž, Pešić M. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar, Milinčić, Danijel, Nedić, Nebojša, Gašić, Uroš, Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav, Pešić, Mirjana, "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Milinčić, Danijel
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš
AU  - Lević, Steva
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Nedović, Viktor
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4316
AB  - The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties
IS  - 7
VL  - 11
DO  - 10.3390/biom11070965
SP  - 965
ER  - 

@article{
author = "Milinčić, Danijel and Kostić, Aleksandar and Gašić, Uroš and Lević, Steva and Stanojević, Sladjana and Barać, Miroljub and Tešić, Živoslav and Nedović, Viktor and Pešić, Mirjana",
year = "2021",
abstract = "The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties",
number = "7",
volume = "11",
doi = "10.3390/biom11070965",
pages = "965"
}

Milinčić, D., Kostić, A., Gašić, U., Lević, S., Stanojević, S., Barać, M., Tešić, Ž., Nedović, V.,& Pešić, M.. (2021). Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules
Basel: MDPI., 11(7), 965.
https://doi.org/10.3390/biom11070965

Milinčić D, Kostić A, Gašić U, Lević S, Stanojević S, Barać M, Tešić Ž, Nedović V, Pešić M. Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules. 2021;11(7):965.
doi:10.3390/biom11070965 .

Milinčić, Danijel, Kostić, Aleksandar, Gašić, Uroš, Lević, Steva, Stanojević, Sladjana, Barać, Miroljub, Tešić, Živoslav, Nedović, Viktor, Pešić, Mirjana, "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties" in Biomolecules, 11, no. 7 (2021):965,
https://doi.org/10.3390/biom11070965 . .

TY  - CONF
AU  - Vuković, Sandra
AU  - Milinčić, Danijel
AU  - Mačukanović-Jocić, Marina
AU  - Kilibarda, Sofija
AU  - Jarić, Snežana
AU  - Kostić, Aleksandar
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4907
AB  - Corydalis ochroleuca Koch. (Papaveraceae) called white corydalis is herbaceous
perennial plant, native to rocky woodland areas of south-eastern Europe. It is recognized by narrow,
tubular, fragrant white-yellow flowers. Currently, use of this plant is mainly associated with decorative
purposes, while there is lack of data for other possibilities of its application.
In this research, rhizome and vegetative shoot of C. ochroleuca collected from canyon of the Derventa
river (National park Tara) located in western Serbia, were used to determine antioxidant activity by
ABTS∙+, DPPH∙, in vitro phosphomolybdenum total antioxidant (TAC), cupric reducing antioxidant
capacity (CUPRAC) and ferric reducing power (FRP) assays. For this purpose, samples were washed,
powdered and extracted with 80% methanol by solvent extraction (SE) and ultrasound-assisted extraction
(UAE).
The aim of this study was to evaluate antioxidant activity of C. ochroleuca extracts and to determine
their correlation with previously spectrophotometrically determined phenolic classes (total phenolics
(TPC), total flavonoids (TFC), total hydroxycinnamic acid derivatives (HCAs)).
The results of antioxidat assays show that the rhizome extract obtained by UAE show the highest
antioxidant activity by ABTS∙+ (58.66% ± 0.72) and CUPRAC (67.30 ± 0.73 mg/g AAE). In the case of
DPPH∙, TAC and FRP assays, extracts of vegetative shoot obtained by both extraction techniques (SE
and UAE) show higher antioxidant activity then rhizome extracts.
Correlation analysis confirmed a positive correlation between TFC and antioxidant tests - DPPH∙ (r=
0.96; p˂ 0.1) and TAC (r= 0.92; p˂ 0.1), and between HCAs and DPPH∙ (r= 0.91; p˂ 0.1), respectively.
DPPH∙ is a known lipophilic and stable free radical, so this result indicates that the extract of the tested
plant was rich in lipophilic flavonoids and chlorogenic acid derivatives that are able to donate H+ ions
and neutralize the DPPH radical.
Conclusion. Also, there was good correlation between DPPH∙ and TAC (r= 0.99; p˂ 0.1). It is assumed
that the total flavonoids, which show the same activity according to these assays, contributed to this
correlation.
In general, flavonoids apparently contribute to high antioxidant activity of C. ochroleuca which indicate
a complex flavonoid profile. Further research should focus on detailed phenolic characterization and
analysis with their in vivo and in vitro properties.
PB  - Kharkiv, Ukraine: National University of Pharmacy, Pharmaceutical Chemistry Department
C3  - International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine
T1  - Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4907
ER  - 

@conference{
author = "Vuković, Sandra and Milinčić, Danijel and Mačukanović-Jocić, Marina and Kilibarda, Sofija and Jarić, Snežana and Kostić, Aleksandar",
year = "2021",
abstract = "Corydalis ochroleuca Koch. (Papaveraceae) called white corydalis is herbaceous
perennial plant, native to rocky woodland areas of south-eastern Europe. It is recognized by narrow,
tubular, fragrant white-yellow flowers. Currently, use of this plant is mainly associated with decorative
purposes, while there is lack of data for other possibilities of its application.
In this research, rhizome and vegetative shoot of C. ochroleuca collected from canyon of the Derventa
river (National park Tara) located in western Serbia, were used to determine antioxidant activity by
ABTS∙+, DPPH∙, in vitro phosphomolybdenum total antioxidant (TAC), cupric reducing antioxidant
capacity (CUPRAC) and ferric reducing power (FRP) assays. For this purpose, samples were washed,
powdered and extracted with 80% methanol by solvent extraction (SE) and ultrasound-assisted extraction
(UAE).
The aim of this study was to evaluate antioxidant activity of C. ochroleuca extracts and to determine
their correlation with previously spectrophotometrically determined phenolic classes (total phenolics
(TPC), total flavonoids (TFC), total hydroxycinnamic acid derivatives (HCAs)).
The results of antioxidat assays show that the rhizome extract obtained by UAE show the highest
antioxidant activity by ABTS∙+ (58.66% ± 0.72) and CUPRAC (67.30 ± 0.73 mg/g AAE). In the case of
DPPH∙, TAC and FRP assays, extracts of vegetative shoot obtained by both extraction techniques (SE
and UAE) show higher antioxidant activity then rhizome extracts.
Correlation analysis confirmed a positive correlation between TFC and antioxidant tests - DPPH∙ (r=
0.96; p˂ 0.1) and TAC (r= 0.92; p˂ 0.1), and between HCAs and DPPH∙ (r= 0.91; p˂ 0.1), respectively.
DPPH∙ is a known lipophilic and stable free radical, so this result indicates that the extract of the tested
plant was rich in lipophilic flavonoids and chlorogenic acid derivatives that are able to donate H+ ions
and neutralize the DPPH radical.
Conclusion. Also, there was good correlation between DPPH∙ and TAC (r= 0.99; p˂ 0.1). It is assumed
that the total flavonoids, which show the same activity according to these assays, contributed to this
correlation.
In general, flavonoids apparently contribute to high antioxidant activity of C. ochroleuca which indicate
a complex flavonoid profile. Further research should focus on detailed phenolic characterization and
analysis with their in vivo and in vitro properties.",
publisher = "Kharkiv, Ukraine: National University of Pharmacy, Pharmaceutical Chemistry Department",
journal = "International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine",
title = "Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4907"
}

Vuković, S., Milinčić, D., Mačukanović-Jocić, M., Kilibarda, S., Jarić, S.,& Kostić, A.. (2021). Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques. in International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine
Kharkiv, Ukraine: National University of Pharmacy, Pharmaceutical Chemistry Department., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_4907

Vuković S, Milinčić D, Mačukanović-Jocić M, Kilibarda S, Jarić S, Kostić A. Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques. in International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine. 2021;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_4907 .

Vuković, Sandra, Milinčić, Danijel, Mačukanović-Jocić, Marina, Kilibarda, Sofija, Jarić, Snežana, Kostić, Aleksandar, "Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques" in International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine (2021):79,
https://hdl.handle.net/21.15107/rcub_ibiss_4907 .

TY  - CONF
AU  - Kostić, Aleksandar Ž.
AU  - Kilibarda, Sofija N.
AU  - Jarić, Snežana
AU  - Mačukanović-Jocić, Marina P.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4902
AB  - Phytochemical analysis with determination of the content of secondary plant metabolites is still very
popular research topic. The aim of present study was to determine the content of selected bioactive
compounds in rhizome and aerial vegetative parts of the plant Corydalis ochroleuca. Plant material
was collected in Derventa river canyon (Serbia). Extraction (1.5 g) was performed with 80%
methanol (15 mL) as solvent for 3 h. Extracts were stored at cold and dark place until further
analysis. The content of total phenolics (TPC), flavonoids (TFC), and hydroxycinnamic acid
derivatives (THD) was determined via standard spectrophotometric methods and expressed as mg/g
per dry weights of galic acid (GAE), quercetin (QE) and chlorogenic acid (CGAE) equivalents,
respectively. It was observed that TPC, TFC and THD values were higher in vegetative part (3.45
mg/g GAE; 1.18 mg/g QE; 1.50 mg/g CGAE) compared to rhizome (2.54 mg/g GAE; 0.28 mg/g
QE; 1.28 mg/g CGAE). It can be concluded that aerial vegetative parts of C. ochroleuca are better
source of secondary metabolites compared to rhizome.
AB  - Ispitivanje fitohemijskog sastava biljaka i određivanje sadržaja odabranih sekundarnih metabolita u
njima predstavlja i dalje veoma aktuelnu oblast istraživanja. U okviru ovog rada izvršeno je
ispitivanje sadržaja odabranih sekundarnih metabolita u rizomu i nadzemnom vegetativnom delu
biljke Corydalis ochroleuca. Biljni materijal je sakupljen u kanjonu reke Dervente (Srbija).
Ekstrakcija (1,5 g) je izvršena pomoću 80% metanola (15 mL) u trajanju od 3h, a dobijeni ekstrakti
su čuvani na hladnom i tamnom mestu. Sadržaj ukupnih polifenola (TPC), flavonoida (TFC) i
derivata dihidroksicimetne kiseline (THD) je utvrđen standardnim spektrofotometrijskim metodama
i izražen kao mg/g ekvivalenata galne kiseline (GAE), kvercetina (QE) i hlorogenske kiseline
(CGAE) na suvu masu uzorka. Utvrđeno je da nadzemni deo biljke sadrži više bioaktivnih
jedinjenja (3,45 mg/g GAE; 1,18 mg/g QE; 1,50 mg/g CGAE) u odnosu na rizom biljke (2,54 mg/g
GAE; 0,28 mg/g QE; 1,28 mg/g CGAE). Na osnovu dobijenih rezultata može se reći da je nadzemni
vegetativni deo biljke C. ochroleuca bolji izvor odabranih sekundarnih metabolita u odnosu na
rizom.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of Abstracts and Proceedings: 57th meeting of the Serbian Chemical Society; 2021 Jun 18-19; Kragujevac, Serbia.
T1  - The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca
T1  - Sadržaj ukupnih polifenola, flavonoida i derivata dihidroksicimetne kiseline u rizomu i vegetativnom delu biljke Corydalis ochroleuca
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4902
ER  - 

@conference{
author = "Kostić, Aleksandar Ž. and Kilibarda, Sofija N. and Jarić, Snežana and Mačukanović-Jocić, Marina P.",
year = "2021",
abstract = "Phytochemical analysis with determination of the content of secondary plant metabolites is still very
popular research topic. The aim of present study was to determine the content of selected bioactive
compounds in rhizome and aerial vegetative parts of the plant Corydalis ochroleuca. Plant material
was collected in Derventa river canyon (Serbia). Extraction (1.5 g) was performed with 80%
methanol (15 mL) as solvent for 3 h. Extracts were stored at cold and dark place until further
analysis. The content of total phenolics (TPC), flavonoids (TFC), and hydroxycinnamic acid
derivatives (THD) was determined via standard spectrophotometric methods and expressed as mg/g
per dry weights of galic acid (GAE), quercetin (QE) and chlorogenic acid (CGAE) equivalents,
respectively. It was observed that TPC, TFC and THD values were higher in vegetative part (3.45
mg/g GAE; 1.18 mg/g QE; 1.50 mg/g CGAE) compared to rhizome (2.54 mg/g GAE; 0.28 mg/g
QE; 1.28 mg/g CGAE). It can be concluded that aerial vegetative parts of C. ochroleuca are better
source of secondary metabolites compared to rhizome., Ispitivanje fitohemijskog sastava biljaka i određivanje sadržaja odabranih sekundarnih metabolita u
njima predstavlja i dalje veoma aktuelnu oblast istraživanja. U okviru ovog rada izvršeno je
ispitivanje sadržaja odabranih sekundarnih metabolita u rizomu i nadzemnom vegetativnom delu
biljke Corydalis ochroleuca. Biljni materijal je sakupljen u kanjonu reke Dervente (Srbija).
Ekstrakcija (1,5 g) je izvršena pomoću 80% metanola (15 mL) u trajanju od 3h, a dobijeni ekstrakti
su čuvani na hladnom i tamnom mestu. Sadržaj ukupnih polifenola (TPC), flavonoida (TFC) i
derivata dihidroksicimetne kiseline (THD) je utvrđen standardnim spektrofotometrijskim metodama
i izražen kao mg/g ekvivalenata galne kiseline (GAE), kvercetina (QE) i hlorogenske kiseline
(CGAE) na suvu masu uzorka. Utvrđeno je da nadzemni deo biljke sadrži više bioaktivnih
jedinjenja (3,45 mg/g GAE; 1,18 mg/g QE; 1,50 mg/g CGAE) u odnosu na rizom biljke (2,54 mg/g
GAE; 0,28 mg/g QE; 1,28 mg/g CGAE). Na osnovu dobijenih rezultata može se reći da je nadzemni
vegetativni deo biljke C. ochroleuca bolji izvor odabranih sekundarnih metabolita u odnosu na
rizom.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of Abstracts and Proceedings: 57th meeting of the Serbian Chemical Society; 2021 Jun 18-19; Kragujevac, Serbia.",
title = "The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca, Sadržaj ukupnih polifenola, flavonoida i derivata dihidroksicimetne kiseline u rizomu i vegetativnom delu biljke Corydalis ochroleuca",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4902"
}

Kostić, A. Ž., Kilibarda, S. N., Jarić, S.,& Mačukanović-Jocić, M. P.. (2021). The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca. in Book of Abstracts and Proceedings: 57th meeting of the Serbian Chemical Society; 2021 Jun 18-19; Kragujevac, Serbia.
Belgrade: Serbian Chemical Society., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_4902

Kostić AŽ, Kilibarda SN, Jarić S, Mačukanović-Jocić MP. The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca. in Book of Abstracts and Proceedings: 57th meeting of the Serbian Chemical Society; 2021 Jun 18-19; Kragujevac, Serbia.. 2021;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_4902 .

Kostić, Aleksandar Ž., Kilibarda, Sofija N., Jarić, Snežana, Mačukanović-Jocić, Marina P., "The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca" in Book of Abstracts and Proceedings: 57th meeting of the Serbian Chemical Society; 2021 Jun 18-19; Kragujevac, Serbia. (2021):31,
https://hdl.handle.net/21.15107/rcub_ibiss_4902 .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš
AU  - Pešić, Mirjana B.
AU  - Stanojević, Sladjana P.
AU  - Barać, Miroljub B.
AU  - Mačukanović-Jocić, Marina P.
AU  - Avramov, Stevan
AU  - Tešić, Živoslav Lj.
PY  - 2019
UR  - http://doi.wiley.com/10.1002/cbdv.201800565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3286
AB  - This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.
T2  - Chemistry & Biodiversity
T1  - Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species
IS  - 3
VL  - 16
DO  - 10.1002/cbdv.201800565
SP  - e1800565
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Gašić, Uroš and Pešić, Mirjana B. and Stanojević, Sladjana P. and Barać, Miroljub B. and Mačukanović-Jocić, Marina P. and Avramov, Stevan and Tešić, Živoslav Lj.",
year = "2019",
abstract = "This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.",
journal = "Chemistry & Biodiversity",
title = "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species",
number = "3",
volume = "16",
doi = "10.1002/cbdv.201800565",
pages = "e1800565"
}

Kostić, A. Ž., Gašić, U., Pešić, M. B., Stanojević, S. P., Barać, M. B., Mačukanović-Jocić, M. P., Avramov, S.,& Tešić, Ž. Lj.. (2019). Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity, 16(3), e1800565.
https://doi.org/10.1002/cbdv.201800565

Kostić AŽ, Gašić U, Pešić MB, Stanojević SP, Barać MB, Mačukanović-Jocić MP, Avramov S, Tešić ŽL. Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity. 2019;16(3):e1800565.
doi:10.1002/cbdv.201800565 .

Kostić, Aleksandar Ž., Gašić, Uroš, Pešić, Mirjana B., Stanojević, Sladjana P., Barać, Miroljub B., Mačukanović-Jocić, Marina P., Avramov, Stevan, Tešić, Živoslav Lj., "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species" in Chemistry & Biodiversity, 16, no. 3 (2019):e1800565,
https://doi.org/10.1002/cbdv.201800565 . .

TY  - JOUR
AU  - Barac, Miroljub
AU  - Vučić, Tanja
AU  - Zilić, Slađana
AU  - Pešić, Mirjana
AU  - Soković, Marina
AU  - Petrović, Jovana
AU  - Kostić, Aleksandar
AU  - Sredović Ignjatović, Ivana
AU  - Milinčić, Danijel
PY  - 2019
UR  - https://www.mdpi.com/2304-8158/8/3/94
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3313
AB  - This study deals with the effect of in vitro digestion on the functional potential of traditional Serbian white-brined cheeses. The total antioxidant capacity, reducing power and iron (II) chelating properties as well as angiotensin-converting enyzme-inhibitory (ACE-inhibitory) and antimicrobial activities of traditional Serbian white-brined cheeses before and after in vitro digestion were assayed. The traditional cheeses had different antioxidant properties as well as different ACE-inhibitory activities. In vitro digestion improved the total antioxidant capacity (8.42⁻58.56 times) and the reducing power (by 17.90⁻99.30%) of investigated cheeses, whereas their chelating ability was slightly improved or unaffected after digestion. In vitro digestion reduced the ACE-inhibitory potential of water-soluble protein fractions, and digested water-insoluble fractions were the major source of ACE-inhibitory peptides. The digestates did not exhibit any antibacterial potential, whereas they showed moderate antifungal potential toward selected micromycetes. The best antifungal potential had Svrljig ovine cheese and Homolje cow cheese. The results of this study clearly point to a significant functionality of traditional white-brined cheeses.
T2  - Foods (Basel, Switzerland)
T1  - The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses.
IS  - 3
VL  - 8
DO  - 10.3390/foods8030094
SP  - 94
ER  - 

@article{
author = "Barac, Miroljub and Vučić, Tanja and Zilić, Slađana and Pešić, Mirjana and Soković, Marina and Petrović, Jovana and Kostić, Aleksandar and Sredović Ignjatović, Ivana and Milinčić, Danijel",
year = "2019",
abstract = "This study deals with the effect of in vitro digestion on the functional potential of traditional Serbian white-brined cheeses. The total antioxidant capacity, reducing power and iron (II) chelating properties as well as angiotensin-converting enyzme-inhibitory (ACE-inhibitory) and antimicrobial activities of traditional Serbian white-brined cheeses before and after in vitro digestion were assayed. The traditional cheeses had different antioxidant properties as well as different ACE-inhibitory activities. In vitro digestion improved the total antioxidant capacity (8.42⁻58.56 times) and the reducing power (by 17.90⁻99.30%) of investigated cheeses, whereas their chelating ability was slightly improved or unaffected after digestion. In vitro digestion reduced the ACE-inhibitory potential of water-soluble protein fractions, and digested water-insoluble fractions were the major source of ACE-inhibitory peptides. The digestates did not exhibit any antibacterial potential, whereas they showed moderate antifungal potential toward selected micromycetes. The best antifungal potential had Svrljig ovine cheese and Homolje cow cheese. The results of this study clearly point to a significant functionality of traditional white-brined cheeses.",
journal = "Foods (Basel, Switzerland)",
title = "The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses.",
number = "3",
volume = "8",
doi = "10.3390/foods8030094",
pages = "94"
}

Barac, M., Vučić, T., Zilić, S., Pešić, M., Soković, M., Petrović, J., Kostić, A., Sredović Ignjatović, I.,& Milinčić, D.. (2019). The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses.. in Foods (Basel, Switzerland), 8(3), 94.
https://doi.org/10.3390/foods8030094

Barac M, Vučić T, Zilić S, Pešić M, Soković M, Petrović J, Kostić A, Sredović Ignjatović I, Milinčić D. The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses.. in Foods (Basel, Switzerland). 2019;8(3):94.
doi:10.3390/foods8030094 .

Barac, Miroljub, Vučić, Tanja, Zilić, Slađana, Pešić, Mirjana, Soković, Marina, Petrović, Jovana, Kostić, Aleksandar, Sredović Ignjatović, Ivana, Milinčić, Danijel, "The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses." in Foods (Basel, Switzerland), 8, no. 3 (2019):94,
https://doi.org/10.3390/foods8030094 . .

TY  - CONF
AU  - Avramov, Stevan
AU  - Kostić, Aleksandar
AU  - Dojčinović, Biljana
AU  - Milinčić, Danijel
AU  - Mačukanović-Jocić, Marina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6668
AB  - The presence of certain elements in the soil controls their bioavailability to the plant. Uptake
of elements depends on their concentration, solubility of occurring form and soil properties. Also,
the propensity of the plant for the bioaccumulation process will condition the transfer of ions of
elements from the soil to the various parts of the plant. Sometimes good bioaccumulation properties
of plants can be used for their application for bioremediation of polluted areas.
The objective of the present study was to determine the concentrations of selected elements
in the rhizome and above-ground parts of Iris pumila grown in Deliblato Sands (Serbia) in relation
to its surrounding soil. Potassium and selenium contents (mg kg-1of dry matter) were determined
by ICP-OES method after digestion of samples. The concentration, transfer and accumulation of
some elements from the soil to rhizome and above-ground parts were evaluated in terms of Biological
Absorption Coefficient (BAC): cplant part/csoil. The concentration of K+ ions in soil, rhizome,
above-ground vegetative parts and flower were: 1,845.3 mg kg-1, 1,307.0 mg kg-1, 4,461.4 mg kg-1
and 2,875.1 mg kg-1, respectively. The presence of selenium as a trace element was confirmed both
in the soil (0.112 mg kg-1) and in the plant parts (rhizome - 0.156 mg kg-1; above-ground vegetative parts- 0.135 mg kg-1; flower - 0.122 mg kg-1). The obtained results confirmed that the aboveground
parts accumulated potassium (BACvegetative= 2.42; BACflowers= 1.56). Also, all plant parts have
shown the ability to bond selenium from soil in significant quantities (BACrhizomes= 1.38; BACvegetative= 1.20; BACflowers= 1.08). This suggests that this specie have potential in phytoremediation.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Bioaccumulation abilities of different parts of Iris pumila L.
SP  - 125
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6668
ER  - 

@conference{
author = "Avramov, Stevan and Kostić, Aleksandar and Dojčinović, Biljana and Milinčić, Danijel and Mačukanović-Jocić, Marina",
year = "2018",
abstract = "The presence of certain elements in the soil controls their bioavailability to the plant. Uptake
of elements depends on their concentration, solubility of occurring form and soil properties. Also,
the propensity of the plant for the bioaccumulation process will condition the transfer of ions of
elements from the soil to the various parts of the plant. Sometimes good bioaccumulation properties
of plants can be used for their application for bioremediation of polluted areas.
The objective of the present study was to determine the concentrations of selected elements
in the rhizome and above-ground parts of Iris pumila grown in Deliblato Sands (Serbia) in relation
to its surrounding soil. Potassium and selenium contents (mg kg-1of dry matter) were determined
by ICP-OES method after digestion of samples. The concentration, transfer and accumulation of
some elements from the soil to rhizome and above-ground parts were evaluated in terms of Biological
Absorption Coefficient (BAC): cplant part/csoil. The concentration of K+ ions in soil, rhizome,
above-ground vegetative parts and flower were: 1,845.3 mg kg-1, 1,307.0 mg kg-1, 4,461.4 mg kg-1
and 2,875.1 mg kg-1, respectively. The presence of selenium as a trace element was confirmed both
in the soil (0.112 mg kg-1) and in the plant parts (rhizome - 0.156 mg kg-1; above-ground vegetative parts- 0.135 mg kg-1; flower - 0.122 mg kg-1). The obtained results confirmed that the aboveground
parts accumulated potassium (BACvegetative= 2.42; BACflowers= 1.56). Also, all plant parts have
shown the ability to bond selenium from soil in significant quantities (BACrhizomes= 1.38; BACvegetative= 1.20; BACflowers= 1.08). This suggests that this specie have potential in phytoremediation.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Bioaccumulation abilities of different parts of Iris pumila L.",
pages = "125-126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6668"
}

Avramov, S., Kostić, A., Dojčinović, B., Milinčić, D.,& Mačukanović-Jocić, M.. (2018). Bioaccumulation abilities of different parts of Iris pumila L.. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Beograd: Serbian Plant Physiology Society., 125-126.
https://hdl.handle.net/21.15107/rcub_ibiss_6668

Avramov S, Kostić A, Dojčinović B, Milinčić D, Mačukanović-Jocić M. Bioaccumulation abilities of different parts of Iris pumila L.. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:125-126.
https://hdl.handle.net/21.15107/rcub_ibiss_6668 .

Avramov, Stevan, Kostić, Aleksandar, Dojčinović, Biljana, Milinčić, Danijel, Mačukanović-Jocić, Marina, "Bioaccumulation abilities of different parts of Iris pumila L." in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):125-126,
https://hdl.handle.net/21.15107/rcub_ibiss_6668 .

TY  - CONF
AU  - Milinčić, Danijel
AU  - Avramov, Stevan
AU  - Mačukanović-Jocić, Marina
AU  - Dojčinović, Biljana
AU  - Stanojević, Slađana
AU  - Pešić, Mirjana
AU  - Kostić, Aleksandar
PY  - 2018
UR  - https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2018_42_2_1_full.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6678
AB  - The mineral composition of the plant mainly depends on two factors – the growing soil composition and/or the influence of the environment (especially anthropogenic factor). Availability of metal’s ions in soil for plant root is determined by element form, pH-value, etc. Some plants possessed ability to concentrate (bio accumulate) some macro- or microelements. The aim of this work was to examine ability of diff erent parts of Iris humillis plant (rhizome, above-ground vegetative part and flower) to bound metal ions from surrounding growing soil or environment. Th is process was expressed as Biological
Absorption Coefficient (BAC) which was calculated as cplant part/csoil ratio where cplant part and csoil are concentration of selected element in different parts of plant i.e. in soil. Content of selected elements (potassium, phosphorus and nickel) in soil and plant was determined by ICP-OES method (including
pre-digestion of samples) and expressed as mg/kg of dry matter. Determined concentrations of potassium, phosphorus and nickel in soil were 877.1 mg/kg, 352.2 mg/kg and 2.97 mg/kg respectively. Content of selected elements in different parts of I. humillis was as follow: rhizome (K- 1686.0 mg/kg,
P- 474.2 mg/kg and Ni- 8.5 mg/kg), above-ground vegetative parts (K- 3033.1 mg/kg, P- 423.1 mg/kg and Ni- 0.29 mg/kg) and flower (K- 1461.4 mg/kg, P- 273.0 mg/kg and Ni- 0.12 mg/kg). According to results for BAC potassium was bioaccumulate in all plant parts since value of this factor was higher than 1 (BACrhizome= 1.92, BACabove-ground vegetative parts =3.46, BACflower= 1.67). Also, rhizome and above-ground vegetative part of I. humillis showed ability for bioaccumulation of phosphorus (BACrhizome= 1.35, BACabove-ground vegetative parts =1.20, BACflower= 0.78) while rhizome possessed high ability to concentrate nickel (BACrhizome= 2.86). The obtained results indicated
that this Iris species possessed good ability for Ni-bonding through rhizome. It can be important for potential bioremediation use, since increased nickel concentration in the environment
can be caused by some anthropogenic sources.
PB  - Beograd: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade
C3  - Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1)
T1  - Bioacumulation capacity of Iris humilis
SP  - 35
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6678
ER  - 

@conference{
author = "Milinčić, Danijel and Avramov, Stevan and Mačukanović-Jocić, Marina and Dojčinović, Biljana and Stanojević, Slađana and Pešić, Mirjana and Kostić, Aleksandar",
year = "2018",
abstract = "The mineral composition of the plant mainly depends on two factors – the growing soil composition and/or the influence of the environment (especially anthropogenic factor). Availability of metal’s ions in soil for plant root is determined by element form, pH-value, etc. Some plants possessed ability to concentrate (bio accumulate) some macro- or microelements. The aim of this work was to examine ability of diff erent parts of Iris humillis plant (rhizome, above-ground vegetative part and flower) to bound metal ions from surrounding growing soil or environment. Th is process was expressed as Biological
Absorption Coefficient (BAC) which was calculated as cplant part/csoil ratio where cplant part and csoil are concentration of selected element in different parts of plant i.e. in soil. Content of selected elements (potassium, phosphorus and nickel) in soil and plant was determined by ICP-OES method (including
pre-digestion of samples) and expressed as mg/kg of dry matter. Determined concentrations of potassium, phosphorus and nickel in soil were 877.1 mg/kg, 352.2 mg/kg and 2.97 mg/kg respectively. Content of selected elements in different parts of I. humillis was as follow: rhizome (K- 1686.0 mg/kg,
P- 474.2 mg/kg and Ni- 8.5 mg/kg), above-ground vegetative parts (K- 3033.1 mg/kg, P- 423.1 mg/kg and Ni- 0.29 mg/kg) and flower (K- 1461.4 mg/kg, P- 273.0 mg/kg and Ni- 0.12 mg/kg). According to results for BAC potassium was bioaccumulate in all plant parts since value of this factor was higher than 1 (BACrhizome= 1.92, BACabove-ground vegetative parts =3.46, BACflower= 1.67). Also, rhizome and above-ground vegetative part of I. humillis showed ability for bioaccumulation of phosphorus (BACrhizome= 1.35, BACabove-ground vegetative parts =1.20, BACflower= 0.78) while rhizome possessed high ability to concentrate nickel (BACrhizome= 2.86). The obtained results indicated
that this Iris species possessed good ability for Ni-bonding through rhizome. It can be important for potential bioremediation use, since increased nickel concentration in the environment
can be caused by some anthropogenic sources.",
publisher = "Beograd: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade",
journal = "Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1)",
title = "Bioacumulation capacity of Iris humilis",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6678"
}

Milinčić, D., Avramov, S., Mačukanović-Jocić, M., Dojčinović, B., Stanojević, S., Pešić, M.,& Kostić, A.. (2018). Bioacumulation capacity of Iris humilis. in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1)
Beograd: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade., 35-35.
https://hdl.handle.net/21.15107/rcub_ibiss_6678

Milinčić D, Avramov S, Mačukanović-Jocić M, Dojčinović B, Stanojević S, Pešić M, Kostić A. Bioacumulation capacity of Iris humilis. in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1). 2018;:35-35.
https://hdl.handle.net/21.15107/rcub_ibiss_6678 .

Milinčić, Danijel, Avramov, Stevan, Mačukanović-Jocić, Marina, Dojčinović, Biljana, Stanojević, Slađana, Pešić, Mirjana, Kostić, Aleksandar, "Bioacumulation capacity of Iris humilis" in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1) (2018):35-35,
https://hdl.handle.net/21.15107/rcub_ibiss_6678 .