TY  - CONF
AU  - Cvetić Antić, Tijana
AU  - Janošević, Dušica
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6503
AB  - Fungi and plants interact in different ways, creating a scale of associations. Hemibiotrophic
fungi represent the most interesting group, as they use sequential biotrophic and necrotrophic infection strategies. The co-evolution of plant and fungal life-styles has not been well characterized.
We present changes of enzymatic component of antioxidative system in succulent plant Tacitus
bellus that specifically correspond to subsequent phases (spore germination, biotrophic phase,
and necrotrophic phase) of hemibiotroph Fusarium verticillioides infection. T. bellus response to F.
verticillioides spore germination was characterized by transient increase in catalase (CAT), but decrease in superoxide dismutase (SOD) and peroxidase (POD) activity. During biotrophic phase of F.
verticillioides infection, when hyphae spread intercellularly in epidermal and mesophyll tissue, host
antioxidative system was suppressed. The transition from biotrophic to necrotrophic phase (inter
and intracellular colonization and sporulation) triggered the host plant cells to create a highly defensive environment: CAT, SOD and POD activities were significantly stimulated, slowing, or even
currently arresting, colonization of T. bellus mesophyll cells. CAT, showing the most pronounced
activity increase, could be suggested as the main enzyme responsible for slowing the progression
of necrotrophic phase of F. verticillioides growth. However, contrary to host CAT and SOD which
isoenzyme profile didn’t change, new highly acidic POD isoforms replaced the two mildly acidic
isoforms, suggesting their specific role in slowing the progression of infection. Presented results
add to knowledge of events and mechanisms related to hemibiotrophic fungi pathogenicity in
succulent plants grown under high relative humidity, similar to conditions in greenhouse.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6503
ER  - 

@conference{
author = "Cvetić Antić, Tijana and Janošević, Dušica and Glamočlija, Jasmina and Mitrović, Aleksandra",
year = "2022",
abstract = "Fungi and plants interact in different ways, creating a scale of associations. Hemibiotrophic
fungi represent the most interesting group, as they use sequential biotrophic and necrotrophic infection strategies. The co-evolution of plant and fungal life-styles has not been well characterized.
We present changes of enzymatic component of antioxidative system in succulent plant Tacitus
bellus that specifically correspond to subsequent phases (spore germination, biotrophic phase,
and necrotrophic phase) of hemibiotroph Fusarium verticillioides infection. T. bellus response to F.
verticillioides spore germination was characterized by transient increase in catalase (CAT), but decrease in superoxide dismutase (SOD) and peroxidase (POD) activity. During biotrophic phase of F.
verticillioides infection, when hyphae spread intercellularly in epidermal and mesophyll tissue, host
antioxidative system was suppressed. The transition from biotrophic to necrotrophic phase (inter
and intracellular colonization and sporulation) triggered the host plant cells to create a highly defensive environment: CAT, SOD and POD activities were significantly stimulated, slowing, or even
currently arresting, colonization of T. bellus mesophyll cells. CAT, showing the most pronounced
activity increase, could be suggested as the main enzyme responsible for slowing the progression
of necrotrophic phase of F. verticillioides growth. However, contrary to host CAT and SOD which
isoenzyme profile didn’t change, new highly acidic POD isoforms replaced the two mildly acidic
isoforms, suggesting their specific role in slowing the progression of infection. Presented results
add to knowledge of events and mechanisms related to hemibiotrophic fungi pathogenicity in
succulent plants grown under high relative humidity, similar to conditions in greenhouse.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6503"
}

Cvetić Antić, T., Janošević, D., Glamočlija, J.,& Mitrović, A.. (2022). Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 50.
https://hdl.handle.net/21.15107/rcub_ibiss_6503

Cvetić Antić T, Janošević D, Glamočlija J, Mitrović A. Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:50.
https://hdl.handle.net/21.15107/rcub_ibiss_6503 .

Cvetić Antić, Tijana, Janošević, Dušica, Glamočlija, Jasmina, Mitrović, Aleksandra, "Enzymatic component of antioxidative system in succulent plant Tacitus bellus as a response to hemibiotroph Fusarium verticillioides infection in vitro" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):50,
https://hdl.handle.net/21.15107/rcub_ibiss_6503 .

TY  - JOUR
AU  - Mitrović, Aleksandra
AU  - Janošević, Dušica A.
AU  - Budimir, Snežana
AU  - Bogdanović-Pristov, Jelena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1181
AB  - Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.
T2  - Biologia Plantarum
T1  - Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis
IS  - 2
VL  - 56
EP  - 361
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1181
ER  - 

@article{
author = "Mitrović, Aleksandra and Janošević, Dušica A. and Budimir, Snežana and Bogdanović-Pristov, Jelena",
year = "2012",
abstract = "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.",
journal = "Biologia Plantarum",
title = "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis",
number = "2",
volume = "56",
pages = "361",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1181"
}

Mitrović, A., Janošević, D. A., Budimir, S.,& Bogdanović-Pristov, J.. (2012). Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. in Biologia Plantarum, 56(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1181

Mitrović A, Janošević DA, Budimir S, Bogdanović-Pristov J. Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. in Biologia Plantarum. 2012;56(2):null-361.
https://hdl.handle.net/21.15107/rcub_ibiss_1181 .

Mitrović, Aleksandra, Janošević, Dušica A., Budimir, Snežana, Bogdanović-Pristov, Jelena, "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis" in Biologia Plantarum, 56, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1181 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Mitrović, Aleksandra
AU  - Ćulafić, Ljubinka
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1704
AB  - In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.
T2  - Biologia Plantarum
T1  - Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro
IS  - 1
VL  - 49
DO  - 10.1007/s00000-005-5039-5
SP  - 35
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1704
ER  - 

@article{
author = "Dmitrović, Slavica and Mitrović, Aleksandra and Ćulafić, Ljubinka",
year = "2005",
abstract = "In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.",
journal = "Biologia Plantarum",
title = "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro",
number = "1",
volume = "49",
doi = "10.1007/s00000-005-5039-5",
pages = "35-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1704"
}

Dmitrović, S., Mitrović, A.,& Ćulafić, L.. (2005). Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum, 49(1), 35-39.
https://doi.org/10.1007/s00000-005-5039-5
https://hdl.handle.net/21.15107/rcub_ibiss_1704

Dmitrović S, Mitrović A, Ćulafić L. Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum. 2005;49(1):35-39.
doi:10.1007/s00000-005-5039-5
https://hdl.handle.net/21.15107/rcub_ibiss_1704 .

Dmitrović, Slavica, Mitrović, Aleksandra, Ćulafić, Ljubinka, "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro" in Biologia Plantarum, 49, no. 1 (2005):35-39,
https://doi.org/10.1007/s00000-005-5039-5 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1704 .

TY  - JOUR
AU  - Mitrović, Aleksandra
AU  - Vinterhalter, Branka
AU  - Ćulafić, Ljubinka
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/358
AB  - Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized.
AB  - Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae je pronađena 1972 godine u planinskim regionima Meksika i jugozapadne Amerike. Rozete, 10 cm u prečniku, leže gotovo na zemlji. Stabljike sa mnogobrojnim zvezdolikim ružičastim cvetovima, pojavljuju u maju i junu, što ovu biljku čini jednom od najprivlačnijih sukulentnih biljaka. Površinski sterilisani donji listovi rozete su postavljani na podlogu koja sadrži MS mineralni i vitaminski rastvor, saharozu (3 %), BAP (2.25 mg dm-3) i NAA (0.186 mg dm-3) i agar (0.7 %). Posle šest meseci kultivacije na dvofaznom medijumu, primećen je kalus i veliki broj pupoljaka u osnovi eksplantata. Medijumi za multiplikaciju pupoljaka sadržali su BAP (0.1 - 0.5 mg dm-3) i NAA (0.1 mg dm-3), dok su medijumi za ožiljavanje malih rozta sadržali NAA (0.1 ili 0.2 mg dm-3). Spontano ožiljavanje (80 %) je uočeno na podlozi bez hormona, dok je NAA (0.1 i 0.2 mg dm-3) stimulisala ožiljavanje do 100%, u zavisnosti od klona. Ožiljene biljke su lako aklimatizovane.
T2  - Journal of Scientific Agricultural Research
T1  - In vitro propagacija Tacitus bellus
T1  - In vitro propagation of Tacitus belhts
IS  - 1
VL  - 66
SP  - 33
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_358
ER  - 

@article{
author = "Mitrović, Aleksandra and Vinterhalter, Branka and Ćulafić, Ljubinka",
year = "2005, 2005",
abstract = "Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized., Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae je pronađena 1972 godine u planinskim regionima Meksika i jugozapadne Amerike. Rozete, 10 cm u prečniku, leže gotovo na zemlji. Stabljike sa mnogobrojnim zvezdolikim ružičastim cvetovima, pojavljuju u maju i junu, što ovu biljku čini jednom od najprivlačnijih sukulentnih biljaka. Površinski sterilisani donji listovi rozete su postavljani na podlogu koja sadrži MS mineralni i vitaminski rastvor, saharozu (3 %), BAP (2.25 mg dm-3) i NAA (0.186 mg dm-3) i agar (0.7 %). Posle šest meseci kultivacije na dvofaznom medijumu, primećen je kalus i veliki broj pupoljaka u osnovi eksplantata. Medijumi za multiplikaciju pupoljaka sadržali su BAP (0.1 - 0.5 mg dm-3) i NAA (0.1 mg dm-3), dok su medijumi za ožiljavanje malih rozta sadržali NAA (0.1 ili 0.2 mg dm-3). Spontano ožiljavanje (80 %) je uočeno na podlozi bez hormona, dok je NAA (0.1 i 0.2 mg dm-3) stimulisala ožiljavanje do 100%, u zavisnosti od klona. Ožiljene biljke su lako aklimatizovane.",
journal = "Journal of Scientific Agricultural Research",
title = "In vitro propagacija Tacitus bellus, In vitro propagation of Tacitus belhts",
number = "1",
volume = "66",
pages = "33-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_358"
}

Mitrović, A., Vinterhalter, B.,& Ćulafić, L.. (2005). In vitro propagacija Tacitus bellus. in Journal of Scientific Agricultural Research, 66(1), 33-39.
https://hdl.handle.net/21.15107/rcub_ibiss_358

Mitrović A, Vinterhalter B, Ćulafić L. In vitro propagacija Tacitus bellus. in Journal of Scientific Agricultural Research. 2005;66(1):33-39.
https://hdl.handle.net/21.15107/rcub_ibiss_358 .

Mitrović, Aleksandra, Vinterhalter, Branka, Ćulafić, Ljubinka, "In vitro propagacija Tacitus bellus" in Journal of Scientific Agricultural Research, 66, no. 1 (2005):33-39,
https://hdl.handle.net/21.15107/rcub_ibiss_358 .