TY  - JOUR
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
AU  - Brkljačić, Jelena
AU  - Elaković, Ivana
AU  - Manitašević-Jovanović, Sanja
AU  - Perišić, Tatjana
AU  - Dunđerski, Jadranka S.
AU  - Damjanović, Svetozar S
AU  - Knežević, Goran
AU  - Spirić, Zeljko M
AU  - Vermetten, Eric
AU  - Savić, Danka A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/999
AB  - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
IS  - null
VL  - 43
SP  - 63
EP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_999
ER  - 

@article{
author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Brkljačić, Jelena and Elaković, Ivana and Manitašević-Jovanović, Sanja and Perišić, Tatjana and Dunđerski, Jadranka S. and Damjanović, Svetozar S and Knežević, Goran and Spirić, Zeljko M and Vermetten, Eric and Savić, Danka A",
year = "2013",
abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD",
number = "null",
volume = "43",
pages = "63-245",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_999"
}

Matić, G., Vojnović-Milutinović, D., Brkljačić, J., Elaković, I., Manitašević-Jovanović, S., Perišić, T., Dunđerski, J. S., Damjanović, S. S., Knežević, G., Spirić, Z. M., Vermetten, E.,& Savić, D. A.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43(null), 63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999

Matić G, Vojnović-Milutinović D, Brkljačić J, Elaković I, Manitašević-Jovanović S, Perišić T, Dunđerski JS, Damjanović SS, Knežević G, Spirić ZM, Vermetten E, Savić DA. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2013;43(null):63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999 .

Matić, Gordana, Vojnović-Milutinović, Danijela, Brkljačić, Jelena, Elaković, Ivana, Manitašević-Jovanović, Sanja, Perišić, Tatjana, Dunđerski, Jadranka S., Damjanović, Svetozar S, Knežević, Goran, Spirić, Zeljko M, Vermetten, Eric, Savić, Danka A, "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43, no. null (2013):63-245,
https://hdl.handle.net/21.15107/rcub_ibiss_999 .

TY  - JOUR
AU  - Manitašević-Jovanović, Sanja
AU  - Brkljačić, Jelena
AU  - Matić, Gordana
AU  - Tanić, Nikola T
AU  - Vojnović-Milutinović, Danijela
AU  - Elaković, Ivana
AU  - Perišić, Tatjana
AU  - Dunđerski, Jadranka S.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1388
AB  - Background: Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: beta-actin, glyceraldehyde-3-phosphate dehydrogenase, beta(2)-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD. Results: The variability in expression of the four endogenous controls was assessed by TaqMan Real-time RT-PCR in peripheral blood mononuclear cells from: war veterans with current PTSD, those with lifetime PTSD, trauma controls and healthy subjects. Expression stability was analyzed by GeNorm and NormFinder software packages, and by direct comparison of Ct values. Both, GeNorm and NormFinder identified beta-actin and glyceraldehyde-3-phosphate dehydrogenase as a pair of genes with the lowest stability value. Conclusions: The combination of beta-actin and glyceraldehyde-3-phosphate dehydrogenase appeared to be the most suitable reference for studying alterations in gene expression in peripheral blood mononuclear cells related to vulnerability and resilience to PTSD, as well as to trauma-provoked developing of this disorder and recovery from it. Using glyceraldehyde-3-phosphate dehydrogenase, beta-actin and beta(2)-microglobulin as individual endogenous controls would provide satisfactory data, while RNA polymerase II polypeptide A could not be recommended.
T2  - Bmc Molecular Biology
T1  - Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD
IS  - null
VL  - 11
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1388
ER  - 

@article{
author = "Manitašević-Jovanović, Sanja and Brkljačić, Jelena and Matić, Gordana and Tanić, Nikola T and Vojnović-Milutinović, Danijela and Elaković, Ivana and Perišić, Tatjana and Dunđerski, Jadranka S.",
year = "2010",
abstract = "Background: Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: beta-actin, glyceraldehyde-3-phosphate dehydrogenase, beta(2)-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD. Results: The variability in expression of the four endogenous controls was assessed by TaqMan Real-time RT-PCR in peripheral blood mononuclear cells from: war veterans with current PTSD, those with lifetime PTSD, trauma controls and healthy subjects. Expression stability was analyzed by GeNorm and NormFinder software packages, and by direct comparison of Ct values. Both, GeNorm and NormFinder identified beta-actin and glyceraldehyde-3-phosphate dehydrogenase as a pair of genes with the lowest stability value. Conclusions: The combination of beta-actin and glyceraldehyde-3-phosphate dehydrogenase appeared to be the most suitable reference for studying alterations in gene expression in peripheral blood mononuclear cells related to vulnerability and resilience to PTSD, as well as to trauma-provoked developing of this disorder and recovery from it. Using glyceraldehyde-3-phosphate dehydrogenase, beta-actin and beta(2)-microglobulin as individual endogenous controls would provide satisfactory data, while RNA polymerase II polypeptide A could not be recommended.",
journal = "Bmc Molecular Biology",
title = "Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD",
number = "null",
volume = "11",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1388"
}

Manitašević-Jovanović, S., Brkljačić, J., Matić, G., Tanić, N. T., Vojnović-Milutinović, D., Elaković, I., Perišić, T.,& Dunđerski, J. S.. (2010). Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD. in Bmc Molecular Biology, 11(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1388

Manitašević-Jovanović S, Brkljačić J, Matić G, Tanić NT, Vojnović-Milutinović D, Elaković I, Perišić T, Dunđerski JS. Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD. in Bmc Molecular Biology. 2010;11(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1388 .

Manitašević-Jovanović, Sanja, Brkljačić, Jelena, Matić, Gordana, Tanić, Nikola T, Vojnović-Milutinović, Danijela, Elaković, Ivana, Perišić, Tatjana, Dunđerski, Jadranka S., "Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD" in Bmc Molecular Biology, 11, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1388 .

TY  - JOUR
AU  - Perišić, Tatjana
AU  - Srecković, Miodrag
AU  - Matić, Gordana
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1491
AB  - Background: Previous studies on adult asthmatics have shown that the disease is associated with alterations in glucocorticoid receptor (GR) function. Objectives: The aim of the present study was to examine GR functional properties in adolescent asthma of different severity. Therefore, we determined GR hormone binding parameters, e. g. the equilibrium dissociation constant (K(d)) and the number of binding sites (B(max)), as well as the level of receptor expression in mononuclear cells isolated from the peripheral blood of patients with mild/moderate persistent asthma. Methods: The GR hormone binding activity was assessed by whole-cell binding assay, while receptor expression was evaluated by quantitative Western blotting. Results: In peripheral blood mononuclear cells (PBMCs) of patients suffering from moderate asthma, the GR displayed higher K(d) and B(max) values (78.0 +/- 19.5 nM and 11,715 +/- 1,952 sites/cell, respectively; n = 17) in comparison to healthy subjects (23.8 +/- 7.7 nM and 6,124 +/- 1,369 sites/cell; n = 12) and mild asthmatics (22.4 +/- 8.0 nM and 4,840 +/- 776 sites/cell; n = 11). However, the GR protein level in PBMCs was found to be similar in the three study groups. Conclusion: The results provide data on GR expression and its functional status in inflammatory cells of pediatric asthmatic patients. In moderate asthma, functional characteristics of GR are altered, though the significance of these findings remains to be clarified. Copyright (c) 2008 S. Karger AG, Basel
T2  - Respiration
T1  - Modulation of Glucocorticoid Receptor Function and Expression in Adolescent Moderate Asthma
IS  - 1
VL  - 77
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1491
ER  - 

@article{
author = "Perišić, Tatjana and Srecković, Miodrag and Matić, Gordana",
year = "2009",
abstract = "Background: Previous studies on adult asthmatics have shown that the disease is associated with alterations in glucocorticoid receptor (GR) function. Objectives: The aim of the present study was to examine GR functional properties in adolescent asthma of different severity. Therefore, we determined GR hormone binding parameters, e. g. the equilibrium dissociation constant (K(d)) and the number of binding sites (B(max)), as well as the level of receptor expression in mononuclear cells isolated from the peripheral blood of patients with mild/moderate persistent asthma. Methods: The GR hormone binding activity was assessed by whole-cell binding assay, while receptor expression was evaluated by quantitative Western blotting. Results: In peripheral blood mononuclear cells (PBMCs) of patients suffering from moderate asthma, the GR displayed higher K(d) and B(max) values (78.0 +/- 19.5 nM and 11,715 +/- 1,952 sites/cell, respectively; n = 17) in comparison to healthy subjects (23.8 +/- 7.7 nM and 6,124 +/- 1,369 sites/cell; n = 12) and mild asthmatics (22.4 +/- 8.0 nM and 4,840 +/- 776 sites/cell; n = 11). However, the GR protein level in PBMCs was found to be similar in the three study groups. Conclusion: The results provide data on GR expression and its functional status in inflammatory cells of pediatric asthmatic patients. In moderate asthma, functional characteristics of GR are altered, though the significance of these findings remains to be clarified. Copyright (c) 2008 S. Karger AG, Basel",
journal = "Respiration",
title = "Modulation of Glucocorticoid Receptor Function and Expression in Adolescent Moderate Asthma",
number = "1",
volume = "77",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1491"
}

Perišić, T., Srecković, M.,& Matić, G.. (2009). Modulation of Glucocorticoid Receptor Function and Expression in Adolescent Moderate Asthma. in Respiration, 77(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1491

Perišić T, Srecković M, Matić G. Modulation of Glucocorticoid Receptor Function and Expression in Adolescent Moderate Asthma. in Respiration. 2009;77(1):null-75.
https://hdl.handle.net/21.15107/rcub_ibiss_1491 .

Perišić, Tatjana, Srecković, Miodrag, Matić, Gordana, "Modulation of Glucocorticoid Receptor Function and Expression in Adolescent Moderate Asthma" in Respiration, 77, no. 1 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1491 .

TY  - JOUR
AU  - Perišić, Tatjana
AU  - Srećković, M.
AU  - Matić, Gordana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/204
AB  - It is well known that pathogenesis and maintenance of chronic asthma is associated with alterations of glucocorticoid receptor (GR) function, and also with persistent pulmonary inflammation, the important mediators of which are reactive oxygen and nitrogen species. In this paper, we tested a hypothesis that GR functional alterations in asthma result from the action of oxidants. To that end, we conducted a series of ex vivo treatments of peripheral blood mononuclear cells (PBMCs) of healthy donors with oxidizing agents (3 morpholinosydnonimine, SIN1; S-nitroso-N-acetyl-penicillamine, SNAP; and hydrogen peroxide, H2O2) and compared the resulting GR modifications with those previously noticed in asthmatic patients. The results show that treatment of PBMCs by H2O2 provoked an increase in the level of GR protein, accompanied by a rise in the number of hormone-binding sites and a decline in the receptor's affinity for the hormone. The H2O2 induced changes, including a characteristic GR isoprotein expression pattern, were found to be very similar to the GR changes previously observed in PBMCs of moderate asthmatic patients, but not in mild asthmatics and healthy subjects. Treatment with the other oxidants applied herein produced different effects or exerted no influence on GR. Thus, this study provides preliminary data suggesting that functional alterations of the GR associated with moderate asthma may be mediated by redox mechanisms that are based on oxidative and regulatory actions of H2O2.
AB  - Poznato je da su patogeneza i napredovanje hronične astme povezani sa funkcionalnim promenama glukokortikoidnog receptora (GR) i dugotrajnom inflamacijom disajnih puteva, čiji su medijatori reaktivne vrste kiseonika i azota. U ovom radu testirali smo hipotezu da funkcionalne promene GRu astmi nastaju kao posledica delovanja oksidanata. U tom cilju sproveli smo seriju ex vivo tretmana mononuklearnih ćelija periferne krvi zdravih donora oksidujućim agensima (3-morfolinosidnoniminom, SIN-1; S-nitrozo-N-acetil-penicilaminom, SNAP; i vodonik peroksidom, H2O2) i uporedili nastale modifikacije GR sa onima koje su ranije zapažene kod astmatičnih pacijenata. Rezultati su pokazali da je tretman perifernih limfocita vodonik peroksidom izazvao povećanje koncentracije GR proteina, koje je bilo praćeno povećanjem broja mesta vezivanja hormona i smanjenjem afiniteta receptora prema hormonu. Promene indukovane vodonik peroksidom, uključujući i karakterističnu ekspresiju izoformi GR proteina, bile su vrlo slične promenama koje su ranije zapažene u perifernim limfocitima pacijenata obolelih od srednje teškog oblika astme, ali ne i kod blagih astmatičara i zdravih donora. Tretman drugim primenjenim oksidantima proizveo je drugačije efekte, ili nije imao uticaja na GR. Prema tome, ova studija je dala preliminarne podatke koji ukazuju da su funkcionalne promene GR povezane sa srednje teškom astmom verovatno posredovane redoks mehanizmima koji se zasnivaju na oksidativnoj i regulatornoj ulozi vodonikperoksida.
T2  - Archives of Biological Sciences
T1  - Moguća uloga vodonik peroksida u funkcionalnim promenama glukokortikoidnog receptora u astmi
T1  - Possible role of a hydrogen peroxide-mediated mechanism in glucocorticoid receptor functional alterations associated with moderate asthma
IS  - 4
VL  - 60
SP  - 531
EP  - 539
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_204
ER  - 

@article{
author = "Perišić, Tatjana and Srećković, M. and Matić, Gordana",
year = "2008, 2008",
abstract = "It is well known that pathogenesis and maintenance of chronic asthma is associated with alterations of glucocorticoid receptor (GR) function, and also with persistent pulmonary inflammation, the important mediators of which are reactive oxygen and nitrogen species. In this paper, we tested a hypothesis that GR functional alterations in asthma result from the action of oxidants. To that end, we conducted a series of ex vivo treatments of peripheral blood mononuclear cells (PBMCs) of healthy donors with oxidizing agents (3 morpholinosydnonimine, SIN1; S-nitroso-N-acetyl-penicillamine, SNAP; and hydrogen peroxide, H2O2) and compared the resulting GR modifications with those previously noticed in asthmatic patients. The results show that treatment of PBMCs by H2O2 provoked an increase in the level of GR protein, accompanied by a rise in the number of hormone-binding sites and a decline in the receptor's affinity for the hormone. The H2O2 induced changes, including a characteristic GR isoprotein expression pattern, were found to be very similar to the GR changes previously observed in PBMCs of moderate asthmatic patients, but not in mild asthmatics and healthy subjects. Treatment with the other oxidants applied herein produced different effects or exerted no influence on GR. Thus, this study provides preliminary data suggesting that functional alterations of the GR associated with moderate asthma may be mediated by redox mechanisms that are based on oxidative and regulatory actions of H2O2., Poznato je da su patogeneza i napredovanje hronične astme povezani sa funkcionalnim promenama glukokortikoidnog receptora (GR) i dugotrajnom inflamacijom disajnih puteva, čiji su medijatori reaktivne vrste kiseonika i azota. U ovom radu testirali smo hipotezu da funkcionalne promene GRu astmi nastaju kao posledica delovanja oksidanata. U tom cilju sproveli smo seriju ex vivo tretmana mononuklearnih ćelija periferne krvi zdravih donora oksidujućim agensima (3-morfolinosidnoniminom, SIN-1; S-nitrozo-N-acetil-penicilaminom, SNAP; i vodonik peroksidom, H2O2) i uporedili nastale modifikacije GR sa onima koje su ranije zapažene kod astmatičnih pacijenata. Rezultati su pokazali da je tretman perifernih limfocita vodonik peroksidom izazvao povećanje koncentracije GR proteina, koje je bilo praćeno povećanjem broja mesta vezivanja hormona i smanjenjem afiniteta receptora prema hormonu. Promene indukovane vodonik peroksidom, uključujući i karakterističnu ekspresiju izoformi GR proteina, bile su vrlo slične promenama koje su ranije zapažene u perifernim limfocitima pacijenata obolelih od srednje teškog oblika astme, ali ne i kod blagih astmatičara i zdravih donora. Tretman drugim primenjenim oksidantima proizveo je drugačije efekte, ili nije imao uticaja na GR. Prema tome, ova studija je dala preliminarne podatke koji ukazuju da su funkcionalne promene GR povezane sa srednje teškom astmom verovatno posredovane redoks mehanizmima koji se zasnivaju na oksidativnoj i regulatornoj ulozi vodonikperoksida.",
journal = "Archives of Biological Sciences",
title = "Moguća uloga vodonik peroksida u funkcionalnim promenama glukokortikoidnog receptora u astmi, Possible role of a hydrogen peroxide-mediated mechanism in glucocorticoid receptor functional alterations associated with moderate asthma",
number = "4",
volume = "60",
pages = "531-539",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_204"
}

Perišić, T., Srećković, M.,& Matić, G.. (2008). Moguća uloga vodonik peroksida u funkcionalnim promenama glukokortikoidnog receptora u astmi. in Archives of Biological Sciences, 60(4), 531-539.
https://hdl.handle.net/21.15107/rcub_ibiss_204

Perišić T, Srećković M, Matić G. Moguća uloga vodonik peroksida u funkcionalnim promenama glukokortikoidnog receptora u astmi. in Archives of Biological Sciences. 2008;60(4):531-539.
https://hdl.handle.net/21.15107/rcub_ibiss_204 .

Perišić, Tatjana, Srećković, M., Matić, Gordana, "Moguća uloga vodonik peroksida u funkcionalnim promenama glukokortikoidnog receptora u astmi" in Archives of Biological Sciences, 60, no. 4 (2008):531-539,
https://hdl.handle.net/21.15107/rcub_ibiss_204 .

TY  - JOUR
AU  - Elaković, Ivana
AU  - Perišić, Tatjana
AU  - Canković-Kadijević, Milce
AU  - Matić, Gordana
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1593
AB  - Correlation between the glucocorticoid receptor (GR) number and affinity for the ligand, as well as the relationship between these equilibrium binding parameters and body mass index, blood pressure, and age were examined in peripheral blood mononuclear cells (PBMC) of healthy human subjects. It was found that the only statistically significant correlation was that between the GR number per cell and equilibrium dissociation constant, K-d (r = 0.84, p < 0.0001). This observation implies the existence of a compensatory mechanism providing for lower GR affinity in individuals that have more receptor sites in circulating mononuclear cells and vice versa. This compensatory phenomenon together with considerable interindividual variation (GR number per cell ranging from 1391 to 15133, CV = 58.62%; and K-d from 2.5 to 98.6 nM, CV = 80.87%), reflects plasticity of the glucocorticoid system. The results pose the question of whether this compensatory mechanism observed in healthy human subjects persists in pathophysiological states associated with glucocorticoid hormone actions and suggest that tissue sensitivity to glucocorticoids could be better predicted by the sign and magnitude of the correlation between the two receptor equilibrium binding parameters than by each of them separately. Copyright (C) 2006 John Wiley & Sons, Ltd.
T2  - Cell Biochemistry and Function
T1  - Correlation between glucocorticoid receptor binding parameters, blood pressure, and body mass index in a healthy human population
IS  - 4
VL  - 25
EP  - 431
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1593
ER  - 

@article{
author = "Elaković, Ivana and Perišić, Tatjana and Canković-Kadijević, Milce and Matić, Gordana",
year = "2007",
abstract = "Correlation between the glucocorticoid receptor (GR) number and affinity for the ligand, as well as the relationship between these equilibrium binding parameters and body mass index, blood pressure, and age were examined in peripheral blood mononuclear cells (PBMC) of healthy human subjects. It was found that the only statistically significant correlation was that between the GR number per cell and equilibrium dissociation constant, K-d (r = 0.84, p < 0.0001). This observation implies the existence of a compensatory mechanism providing for lower GR affinity in individuals that have more receptor sites in circulating mononuclear cells and vice versa. This compensatory phenomenon together with considerable interindividual variation (GR number per cell ranging from 1391 to 15133, CV = 58.62%; and K-d from 2.5 to 98.6 nM, CV = 80.87%), reflects plasticity of the glucocorticoid system. The results pose the question of whether this compensatory mechanism observed in healthy human subjects persists in pathophysiological states associated with glucocorticoid hormone actions and suggest that tissue sensitivity to glucocorticoids could be better predicted by the sign and magnitude of the correlation between the two receptor equilibrium binding parameters than by each of them separately. Copyright (C) 2006 John Wiley & Sons, Ltd.",
journal = "Cell Biochemistry and Function",
title = "Correlation between glucocorticoid receptor binding parameters, blood pressure, and body mass index in a healthy human population",
number = "4",
volume = "25",
pages = "431",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1593"
}

Elaković, I., Perišić, T., Canković-Kadijević, M.,& Matić, G.. (2007). Correlation between glucocorticoid receptor binding parameters, blood pressure, and body mass index in a healthy human population. in Cell Biochemistry and Function, 25(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1593

Elaković I, Perišić T, Canković-Kadijević M, Matić G. Correlation between glucocorticoid receptor binding parameters, blood pressure, and body mass index in a healthy human population. in Cell Biochemistry and Function. 2007;25(4):null-431.
https://hdl.handle.net/21.15107/rcub_ibiss_1593 .

Elaković, Ivana, Perišić, Tatjana, Canković-Kadijević, Milce, Matić, Gordana, "Correlation between glucocorticoid receptor binding parameters, blood pressure, and body mass index in a healthy human population" in Cell Biochemistry and Function, 25, no. 4 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1593 .

TY  - JOUR
AU  - Perišić, Tatjana
AU  - Srecković, Miodrag
AU  - Matić, Gordana
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1574
AB  - Objectives: The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma. Design and methods: Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis. Results: Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children. Conclusions: With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
T2  - Clinical Biochemistry
T1  - An imbalance in antioxidant enzymes and stress proteins in childhood asthma
IS  - 15
VL  - 40
EP  - 1171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1574
ER  - 

@article{
author = "Perišić, Tatjana and Srecković, Miodrag and Matić, Gordana",
year = "2007",
abstract = "Objectives: The study was undertaken to examine antioxidant status and level of the major intracellular heat shock proteins (Hsps) in healthy children and children with mild and moderate asthma. Design and methods: Native gel assays were performed to estimate activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD), and catalase (CAT) in peripheral blood mononuclear cells (PBMC) of healthy and asthmatic children. Hsp70 and Hsp90 protein levels in PBMCs were assessed by Western blot analysis. Results: Moderate asthmatics displayed higher CuZnSOD/CAT activity ratio compared to healthy children, and increased Hsp90 level compared to mild asthmatics and healthy children. Conclusions: With regard to the imbalance in the antioxidant enzyme activities children with moderate asthma differ from healthy children, while an increased Hsp90 expression could be associated with the disease severity, as well. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.",
journal = "Clinical Biochemistry",
title = "An imbalance in antioxidant enzymes and stress proteins in childhood asthma",
number = "15",
volume = "40",
pages = "1171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1574"
}

Perišić, T., Srecković, M.,& Matić, G.. (2007). An imbalance in antioxidant enzymes and stress proteins in childhood asthma. in Clinical Biochemistry, 40(15).
https://hdl.handle.net/21.15107/rcub_ibiss_1574

Perišić T, Srecković M, Matić G. An imbalance in antioxidant enzymes and stress proteins in childhood asthma. in Clinical Biochemistry. 2007;40(15):null-1171.
https://hdl.handle.net/21.15107/rcub_ibiss_1574 .

Perišić, Tatjana, Srecković, Miodrag, Matić, Gordana, "An imbalance in antioxidant enzymes and stress proteins in childhood asthma" in Clinical Biochemistry, 40, no. 15 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1574 .

TY  - JOUR
AU  - Perišić, Tatjana
AU  - Srećković, M.
AU  - Matić, Gordana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/179
AB  - The aim of the present study was to examine whether changes in the antioxidant status and expression of major intracellular stress proteins are associated with pathophysiology of childhood asthma and severity of the disease. The activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD) and catalase (CAT) in peripheral blood mononuclear cells (PBMCs) of 28 stable pediatric patients (11 mild persistent and 17 moderate persistent asthmatics) and 12 healthy children were assessed by native gel assays. The levels of two heat shock proteins (HSPs), Hsp70 and Hsp90, were examined by quantitative Western blot analysis. Moderate asthmatics, in comparison to healthy children, displayed higher activity of CuZnSOD, while differences of MnSOD and CAT activity between the groups of patients were not found. The levels of Hsp90 and the inducible isoform of Hsp70 were increased in moderate asthmatics as compared to mild asthmatics and healthy children. The results demonstrate an imbalance in cellular antioxidant and stress response systems that may contribute to pathogenesis of childhood asthma, but are not necessarily related to severity of the disease.
AB  - Cilj ove studije bio je da se ispita da li su promene antioksidativnog statusa i ekspresije glavnih unutar ćelijskih stres proteina povezane sa patogenezom astme i težinom ovog oboljenja kod dece. Aktivnost bakar/cink(CuZn)-i mangan (Mn)-superoksid dismutaze (SOD), i katalaze (CAT) u mononuklearnim ćelijama iz periferne krvi 28 dece sa stabilnom astmom (11 sa blagim i 17 sa srednje teškim oblikom bolesti) i 12 zdrave dece ispitana je enzimskim testom u nativnom gelu. Koncentracije dva ispitivana proteina toplotnog stresa, Hsp70 i Hsp90, određene su tehnikom kvantitativnog imunoblota. Pacijenti sa srednje teškom astmom pokazali su povećanu aktivnost CuZn-SOD upoređenju sa zdravom decom, dok razlike u aktivnosti Mn-SOD i CAT između ispitivanih grupa pacijenata nisu zapažene. Nivo Hsp90 i inducibilne izoforme Hsp70 bio je povećan kod srednje teških astmatičara upoređenju sa blagim astmatičarima i zdravom decom. Rezultati ukazuju da disbalans u ćelijskom antioksidativnom i anti-stres sistemu može doprineti patofiziologiji dečje astme, ali ne mora biti povezan sa težinom oboljenja.
T2  - Archives of Biological Sciences
T1  - Promene aktivnosti antioksidativnih enzima i koncentracije proteina toplotnog stresa u limfocitima dece obolele od astme
T1  - Changes of antioxidant enzyme activity and heat shock protein content in lymphocytes of children with asthma
IS  - 4
VL  - 59
SP  - 257
EP  - 266
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_179
ER  - 

@article{
author = "Perišić, Tatjana and Srećković, M. and Matić, Gordana",
year = "2007, 2007",
abstract = "The aim of the present study was to examine whether changes in the antioxidant status and expression of major intracellular stress proteins are associated with pathophysiology of childhood asthma and severity of the disease. The activities of copper/zinc (CuZn) and manganese (Mn) superoxide dismutase (SOD) and catalase (CAT) in peripheral blood mononuclear cells (PBMCs) of 28 stable pediatric patients (11 mild persistent and 17 moderate persistent asthmatics) and 12 healthy children were assessed by native gel assays. The levels of two heat shock proteins (HSPs), Hsp70 and Hsp90, were examined by quantitative Western blot analysis. Moderate asthmatics, in comparison to healthy children, displayed higher activity of CuZnSOD, while differences of MnSOD and CAT activity between the groups of patients were not found. The levels of Hsp90 and the inducible isoform of Hsp70 were increased in moderate asthmatics as compared to mild asthmatics and healthy children. The results demonstrate an imbalance in cellular antioxidant and stress response systems that may contribute to pathogenesis of childhood asthma, but are not necessarily related to severity of the disease., Cilj ove studije bio je da se ispita da li su promene antioksidativnog statusa i ekspresije glavnih unutar ćelijskih stres proteina povezane sa patogenezom astme i težinom ovog oboljenja kod dece. Aktivnost bakar/cink(CuZn)-i mangan (Mn)-superoksid dismutaze (SOD), i katalaze (CAT) u mononuklearnim ćelijama iz periferne krvi 28 dece sa stabilnom astmom (11 sa blagim i 17 sa srednje teškim oblikom bolesti) i 12 zdrave dece ispitana je enzimskim testom u nativnom gelu. Koncentracije dva ispitivana proteina toplotnog stresa, Hsp70 i Hsp90, određene su tehnikom kvantitativnog imunoblota. Pacijenti sa srednje teškom astmom pokazali su povećanu aktivnost CuZn-SOD upoređenju sa zdravom decom, dok razlike u aktivnosti Mn-SOD i CAT između ispitivanih grupa pacijenata nisu zapažene. Nivo Hsp90 i inducibilne izoforme Hsp70 bio je povećan kod srednje teških astmatičara upoređenju sa blagim astmatičarima i zdravom decom. Rezultati ukazuju da disbalans u ćelijskom antioksidativnom i anti-stres sistemu može doprineti patofiziologiji dečje astme, ali ne mora biti povezan sa težinom oboljenja.",
journal = "Archives of Biological Sciences",
title = "Promene aktivnosti antioksidativnih enzima i koncentracije proteina toplotnog stresa u limfocitima dece obolele od astme, Changes of antioxidant enzyme activity and heat shock protein content in lymphocytes of children with asthma",
number = "4",
volume = "59",
pages = "257-266",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_179"
}

Perišić, T., Srećković, M.,& Matić, G.. (2007). Promene aktivnosti antioksidativnih enzima i koncentracije proteina toplotnog stresa u limfocitima dece obolele od astme. in Archives of Biological Sciences, 59(4), 257-266.
https://hdl.handle.net/21.15107/rcub_ibiss_179

Perišić T, Srećković M, Matić G. Promene aktivnosti antioksidativnih enzima i koncentracije proteina toplotnog stresa u limfocitima dece obolele od astme. in Archives of Biological Sciences. 2007;59(4):257-266.
https://hdl.handle.net/21.15107/rcub_ibiss_179 .

Perišić, Tatjana, Srećković, M., Matić, Gordana, "Promene aktivnosti antioksidativnih enzima i koncentracije proteina toplotnog stresa u limfocitima dece obolele od astme" in Archives of Biological Sciences, 59, no. 4 (2007):257-266,
https://hdl.handle.net/21.15107/rcub_ibiss_179 .

TY  - CONF
AU  - Elaković, Ivana
AU  - Perišić, Tatjana
AU  - Canković-Kadijević, Milce
AU  - Matić, Gordana
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1701
C3  - Febs Journal
T1  - Correlation between glucocorticoid receptor binding parameters, blood pressure and body mass index in healthy human population
IS  - null
VL  - 272
EP  - 483
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1701
ER  - 

@conference{
author = "Elaković, Ivana and Perišić, Tatjana and Canković-Kadijević, Milce and Matić, Gordana",
year = "2005",
journal = "Febs Journal",
title = "Correlation between glucocorticoid receptor binding parameters, blood pressure and body mass index in healthy human population",
number = "null",
volume = "272",
pages = "483",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1701"
}

Elaković, I., Perišić, T., Canković-Kadijević, M.,& Matić, G.. (2005). Correlation between glucocorticoid receptor binding parameters, blood pressure and body mass index in healthy human population. in Febs Journal, 272(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1701

Elaković I, Perišić T, Canković-Kadijević M, Matić G. Correlation between glucocorticoid receptor binding parameters, blood pressure and body mass index in healthy human population. in Febs Journal. 2005;272(null):null-483.
https://hdl.handle.net/21.15107/rcub_ibiss_1701 .

Elaković, Ivana, Perišić, Tatjana, Canković-Kadijević, Milce, Matić, Gordana, "Correlation between glucocorticoid receptor binding parameters, blood pressure and body mass index in healthy human population" in Febs Journal, 272, no. null (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1701 .