TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Renko, Kostja
AU  - Miler, Marko
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Koehrle, Josef
AU  - Milošević, Verica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2333
AB  - We previously reported that orchidectomy (Orx) of middle-aged rats
   (15-16-month-old; MA) slightly affected pituitary-thyroid axis, but
   decreased liver deiodinase (Dio) type I and pituitary Dio2 enzyme
   activities. At present, we examined the effects of subsequent
   testosterone-propionate treatment (5 mg/kg; Orx + T), and compared the
   effects of testosterone with the effects of estradiol-clipropionate
   (0.06 mg/kg; Orx + E) treatment. Hormones were subcutaneously
   administered, daily, for three weeks, while Orx and sham-operated (SO)
   controls received only the vehicle. The applied dose of T did not alter
   serum TSH, T4 and T3 concentrations in Orx- MA, though it increased TSH
   when administrated to Orx young adults (2.5-month-old; Orx-YA). However,
   pituitaries of Orx-MA + T rats had higher relative intensity of
   immunofluorescence (RIP) for TSHI3; in their thyroids we found increased
   volume and height of follicular epithelium, decreased volume of the
   colloid and higher RIF for T,{[}-bound to thyroglobulin (Tg-T4). Liver
   Diol activity was increased. E-treatment did not affect serum hormone
   levels, pituitary RIF for TS1-11i, or liver Diol activity in Orx-MA
   rats. Thyroids had decreased relative volume and height of follicular
   epithelium, increased relative volume of the colloid, decreased volume
   of sodium iodide symporter-immunopositive epithelium and lower RIF for
   Tg-T4. Detected changes were statistically significant. In conclusion,
   androgenization enhanced pituitary TSHr, RIF, thyroid activation and
   liver Diol enzyme activity in Orx-MA, without elevating serum TSH as in
   Orx-YA rats. Estrogenization induced pituitary enlargement with no
   effect on pituitary TSHI) RIF, serum TSH or liver Diol activity. E also
   induced alterations in thyroid histology that indicate mild suppression
   of its functioning, and contributed to thyroid blood vessel enlargement
   in Orx-MA rats. (C) 2015 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Experimental Gerontology
T1  - Testosterone and estradiol treatments differently affect
 pituitary-thyroid axis and liver deiodinase 1 activity in
 orchidectomized middle-aged rats
VL  - 72
DO  - 10.1016/j.exger.2015.09.010
SP  - 85
EP  - 98
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Renko, Kostja and Miler, Marko and Trifunović, Svetlana and Ajdžanović, Vladimir and Koehrle, Josef and Milošević, Verica",
year = "2015",
abstract = "We previously reported that orchidectomy (Orx) of middle-aged rats
   (15-16-month-old; MA) slightly affected pituitary-thyroid axis, but
   decreased liver deiodinase (Dio) type I and pituitary Dio2 enzyme
   activities. At present, we examined the effects of subsequent
   testosterone-propionate treatment (5 mg/kg; Orx + T), and compared the
   effects of testosterone with the effects of estradiol-clipropionate
   (0.06 mg/kg; Orx + E) treatment. Hormones were subcutaneously
   administered, daily, for three weeks, while Orx and sham-operated (SO)
   controls received only the vehicle. The applied dose of T did not alter
   serum TSH, T4 and T3 concentrations in Orx- MA, though it increased TSH
   when administrated to Orx young adults (2.5-month-old; Orx-YA). However,
   pituitaries of Orx-MA + T rats had higher relative intensity of
   immunofluorescence (RIP) for TSHI3; in their thyroids we found increased
   volume and height of follicular epithelium, decreased volume of the
   colloid and higher RIF for T,{[}-bound to thyroglobulin (Tg-T4). Liver
   Diol activity was increased. E-treatment did not affect serum hormone
   levels, pituitary RIF for TS1-11i, or liver Diol activity in Orx-MA
   rats. Thyroids had decreased relative volume and height of follicular
   epithelium, increased relative volume of the colloid, decreased volume
   of sodium iodide symporter-immunopositive epithelium and lower RIF for
   Tg-T4. Detected changes were statistically significant. In conclusion,
   androgenization enhanced pituitary TSHr, RIF, thyroid activation and
   liver Diol enzyme activity in Orx-MA, without elevating serum TSH as in
   Orx-YA rats. Estrogenization induced pituitary enlargement with no
   effect on pituitary TSHI) RIF, serum TSH or liver Diol activity. E also
   induced alterations in thyroid histology that indicate mild suppression
   of its functioning, and contributed to thyroid blood vessel enlargement
   in Orx-MA rats. (C) 2015 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Experimental Gerontology",
title = "Testosterone and estradiol treatments differently affect
 pituitary-thyroid axis and liver deiodinase 1 activity in
 orchidectomized middle-aged rats",
volume = "72",
doi = "10.1016/j.exger.2015.09.010",
pages = "85-98"
}

Šošić-Jurjević, B., Filipović, B., Renko, K., Miler, M., Trifunović, S., Ajdžanović, V., Koehrle, J.,& Milošević, V.. (2015). Testosterone and estradiol treatments differently affect
 pituitary-thyroid axis and liver deiodinase 1 activity in
 orchidectomized middle-aged rats. in Experimental Gerontology
Elsevier., 72, 85-98.
https://doi.org/10.1016/j.exger.2015.09.010

Šošić-Jurjević B, Filipović B, Renko K, Miler M, Trifunović S, Ajdžanović V, Koehrle J, Milošević V. Testosterone and estradiol treatments differently affect
 pituitary-thyroid axis and liver deiodinase 1 activity in
 orchidectomized middle-aged rats. in Experimental Gerontology. 2015;72:85-98.
doi:10.1016/j.exger.2015.09.010 .

Šošić-Jurjević, Branka , Filipović, Branko, Renko, Kostja, Miler, Marko, Trifunović, Svetlana, Ajdžanović, Vladimir, Koehrle, Josef, Milošević, Verica, "Testosterone and estradiol treatments differently affect
 pituitary-thyroid axis and liver deiodinase 1 activity in
 orchidectomized middle-aged rats" in Experimental Gerontology, 72 (2015):85-98,
https://doi.org/10.1016/j.exger.2015.09.010 . .