TY  - CONF
AU  - Lunić, Tanja
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4106
AB  - Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
T1  - Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts
DO  - 10.3390/ECMC2020-07282
ER  - 

@conference{
author = "Lunić, Tanja and Oalđe, Mariana and Mandić, Marija and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.",
title = "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts",
doi = "10.3390/ECMC2020-07282"
}

Lunić, T., Oalđe, M., Mandić, M., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07282

Lunić T, Oalđe M, Mandić M, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.. 2020;.
doi:10.3390/ECMC2020-07282 .

Lunić, Tanja, Oalđe, Mariana, Mandić, Marija, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. (2020),
https://doi.org/10.3390/ECMC2020-07282 . .

TY  - JOUR
AU  - Vujičić, Milorad
AU  - Sabovljević, Aneta
AU  - Sinzar-Sekulić, Jasmina B
AU  - Skorić, Marijana
AU  - Sabovljević, Marko S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1235
AB  - The high mountain pottioid moss Molendoa hornschuchiana (Hook) Lindb. ex Limpr. is a very rare and critically endangered bryophyte species in Europe in need for ex situ conservation. A 25-year-old herbarium sample was used to revive and propagate this species for further reintroduction and introduction to potential natural habitats. The reviving of "dead" herbarium specimen was achieved by disposing of axenical organisms as well as adjusting condition for developing secondary protonema, bud inductions, and optimization of gametophyte propagation in vitro condition. The influence of exogenously added growth regulators on the morphogenesis of this species was studied. The plants were cultured in the two basic types of media, viz., BCD and half-strength Murashige and Skoog (MS) supplemented with different concentrations (0.01-0.3 mu M) of indole-3-butyric acid (IBA) and N-6-benzyladenine (BA) under a 16-h photoperiod. The influence of growth regulators on gametophores multiplication in vitro as well as on protonemal diameter was recorded. Well-developed gametophores were obtained on BCD medium, whereas on half-strength MS medium, secondary protonema was produced, both on hormone-free and supplemented substrate exclusively. Based on multiplication index in vitro, maximum development of gametophores was realized on BCD medium supplemented with 0.3 mu M IBA and 0.1 mu M BA. However, the widest diameter of secondary protonema was obtained on BCD medium enriched with low concentration of both BA (0.01 and 0.03 mu M) and constant concentration of IBA (0.03 mu M). Chemical names used: indole-3-butyric acid (IBA), N-6-benzyladenine (BA), Murashige and Skoog medium (MS).
T2  - Hortscience
T1  - In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta)
IS  - 1
VL  - 47
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1235
ER  - 

@article{
author = "Vujičić, Milorad and Sabovljević, Aneta and Sinzar-Sekulić, Jasmina B and Skorić, Marijana and Sabovljević, Marko S",
year = "2012",
abstract = "The high mountain pottioid moss Molendoa hornschuchiana (Hook) Lindb. ex Limpr. is a very rare and critically endangered bryophyte species in Europe in need for ex situ conservation. A 25-year-old herbarium sample was used to revive and propagate this species for further reintroduction and introduction to potential natural habitats. The reviving of "dead" herbarium specimen was achieved by disposing of axenical organisms as well as adjusting condition for developing secondary protonema, bud inductions, and optimization of gametophyte propagation in vitro condition. The influence of exogenously added growth regulators on the morphogenesis of this species was studied. The plants were cultured in the two basic types of media, viz., BCD and half-strength Murashige and Skoog (MS) supplemented with different concentrations (0.01-0.3 mu M) of indole-3-butyric acid (IBA) and N-6-benzyladenine (BA) under a 16-h photoperiod. The influence of growth regulators on gametophores multiplication in vitro as well as on protonemal diameter was recorded. Well-developed gametophores were obtained on BCD medium, whereas on half-strength MS medium, secondary protonema was produced, both on hormone-free and supplemented substrate exclusively. Based on multiplication index in vitro, maximum development of gametophores was realized on BCD medium supplemented with 0.3 mu M IBA and 0.1 mu M BA. However, the widest diameter of secondary protonema was obtained on BCD medium enriched with low concentration of both BA (0.01 and 0.03 mu M) and constant concentration of IBA (0.03 mu M). Chemical names used: indole-3-butyric acid (IBA), N-6-benzyladenine (BA), Murashige and Skoog medium (MS).",
journal = "Hortscience",
title = "In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta)",
number = "1",
volume = "47",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1235"
}

Vujičić, M., Sabovljević, A., Sinzar-Sekulić, J. B., Skorić, M.,& Sabovljević, M. S.. (2012). In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta). in Hortscience, 47(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1235

Vujičić M, Sabovljević A, Sinzar-Sekulić JB, Skorić M, Sabovljević MS. In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta). in Hortscience. 2012;47(1):null-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1235 .

Vujičić, Milorad, Sabovljević, Aneta, Sinzar-Sekulić, Jasmina B, Skorić, Marijana, Sabovljević, Marko S, "In Vitro Development of the Rare and Endangered Moss Molendoa hornschuchiana (Hook.) Lindb. ex Limpr. (Pottiaceae, Bryophyta)" in Hortscience, 47, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1235 .

TY  - JOUR
AU  - Sabovljević, Marko S
AU  - Vujičić, Milorad
AU  - Sinzar-Sekulić, Jasmina B
AU  - Gabriel, Segarra-Moragues Jose
AU  - Papp, Beata
AU  - Skorić, Marijana
AU  - Dragacević, Luka
AU  - Sabovljević, Aneta
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1111
AB  - This study provides the results of the developmental biology of the highly rare and endangered moss species Brachia vogesiaca (recorded in less than 30 localities in the Northern Hemisphere, mainly western, central, and southwestern Europe). The aim of the study was to achieve the fully developed gametophyte and to propagate it for the purpose of conservation, reintroduction, and introduction to potential habitats free from xenic contamination. These gametophytes will be used for the study of genetics and genomics of this species. The micropropagation of B. vogesiaca was successfully applied on BCD medium supplemented with 0.1 mu M BA and on BCD supplemented with 0.3 mu M IBA and 0.3 mu M BA for numerous gametophore production. The highest production of secondary protonema was achieved on MS/2 S/2 medium enriched with 0.1 or 0.3 mu M IBA and 0.3 mu M BA. Rather successfully applied micropropagation of this threatened moss species enables better knowledge of its biology and is of great value for its conservation biology and developmental research. Chemical names used: indole-3-butyric acid (IBA), N-6-benzyladenine (BA), Murashige and Skoog medium (MS).
T2  - Hortscience
T1  - Reviving, In Vitro Differentiation, Development, and Micropropagation of the Rare and Endangered Moss Bruchia vogesiaca (Bruchiaceae)
IS  - 9
VL  - 47
SP  - 23
EP  - 1350
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1111
ER  - 

@article{
author = "Sabovljević, Marko S and Vujičić, Milorad and Sinzar-Sekulić, Jasmina B and Gabriel, Segarra-Moragues Jose and Papp, Beata and Skorić, Marijana and Dragacević, Luka and Sabovljević, Aneta",
year = "2012",
abstract = "This study provides the results of the developmental biology of the highly rare and endangered moss species Brachia vogesiaca (recorded in less than 30 localities in the Northern Hemisphere, mainly western, central, and southwestern Europe). The aim of the study was to achieve the fully developed gametophyte and to propagate it for the purpose of conservation, reintroduction, and introduction to potential habitats free from xenic contamination. These gametophytes will be used for the study of genetics and genomics of this species. The micropropagation of B. vogesiaca was successfully applied on BCD medium supplemented with 0.1 mu M BA and on BCD supplemented with 0.3 mu M IBA and 0.3 mu M BA for numerous gametophore production. The highest production of secondary protonema was achieved on MS/2 S/2 medium enriched with 0.1 or 0.3 mu M IBA and 0.3 mu M BA. Rather successfully applied micropropagation of this threatened moss species enables better knowledge of its biology and is of great value for its conservation biology and developmental research. Chemical names used: indole-3-butyric acid (IBA), N-6-benzyladenine (BA), Murashige and Skoog medium (MS).",
journal = "Hortscience",
title = "Reviving, In Vitro Differentiation, Development, and Micropropagation of the Rare and Endangered Moss Bruchia vogesiaca (Bruchiaceae)",
number = "9",
volume = "47",
pages = "23-1350",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1111"
}

Sabovljević, M. S., Vujičić, M., Sinzar-Sekulić, J. B., Gabriel, S. J., Papp, B., Skorić, M., Dragacević, L.,& Sabovljević, A.. (2012). Reviving, In Vitro Differentiation, Development, and Micropropagation of the Rare and Endangered Moss Bruchia vogesiaca (Bruchiaceae). in Hortscience, 47(9), 23-1350.
https://hdl.handle.net/21.15107/rcub_ibiss_1111

Sabovljević MS, Vujičić M, Sinzar-Sekulić JB, Gabriel SJ, Papp B, Skorić M, Dragacević L, Sabovljević A. Reviving, In Vitro Differentiation, Development, and Micropropagation of the Rare and Endangered Moss Bruchia vogesiaca (Bruchiaceae). in Hortscience. 2012;47(9):23-1350.
https://hdl.handle.net/21.15107/rcub_ibiss_1111 .

Sabovljević, Marko S, Vujičić, Milorad, Sinzar-Sekulić, Jasmina B, Gabriel, Segarra-Moragues Jose, Papp, Beata, Skorić, Marijana, Dragacević, Luka, Sabovljević, Aneta, "Reviving, In Vitro Differentiation, Development, and Micropropagation of the Rare and Endangered Moss Bruchia vogesiaca (Bruchiaceae)" in Hortscience, 47, no. 9 (2012):23-1350,
https://hdl.handle.net/21.15107/rcub_ibiss_1111 .

TY  - JOUR
AU  - Sabovljević, Aneta
AU  - Vujičić, Milorad
AU  - Skorić, Marijana
AU  - Bajić-Ljubicić, Jasna
AU  - Sabovljević, Marko S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1210
AB  - The study gives the first report of In vitro culture of the moss Thamnobryum alopecurum and the insight into the problems of axenicaly culturing this bryophyte and the conditions for establishment and propagation. This moss is a counterpart of many rare and endangered mosses from the same genus and date presented should be taken into account of conservation and propagation of its counterparts as well. Problems of surface sterilization are elaborated regarding sporophyte vs. gametophyte. The influence of nutrient, light length and temperature on different developmental stages is discussed. The best conditions for micro-propagation from shoots are slightly lower temperature (18-20 degrees C), on BCD medium irrelevant of day length.
T2  - Pakistan Journal of Botany
T1  - Axenically Culturing the Bryophytes: Establishment and Propagation of the Pleurocarpous Moss Thamnobryum Alopecurum Nieuwland Ex Gangulee (Bryophyta, Neckeraceae) in In Vitro Conditions
IS  - 1
VL  - 44
EP  - 344
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1210
ER  - 

@article{
author = "Sabovljević, Aneta and Vujičić, Milorad and Skorić, Marijana and Bajić-Ljubicić, Jasna and Sabovljević, Marko S",
year = "2012",
abstract = "The study gives the first report of In vitro culture of the moss Thamnobryum alopecurum and the insight into the problems of axenicaly culturing this bryophyte and the conditions for establishment and propagation. This moss is a counterpart of many rare and endangered mosses from the same genus and date presented should be taken into account of conservation and propagation of its counterparts as well. Problems of surface sterilization are elaborated regarding sporophyte vs. gametophyte. The influence of nutrient, light length and temperature on different developmental stages is discussed. The best conditions for micro-propagation from shoots are slightly lower temperature (18-20 degrees C), on BCD medium irrelevant of day length.",
journal = "Pakistan Journal of Botany",
title = "Axenically Culturing the Bryophytes: Establishment and Propagation of the Pleurocarpous Moss Thamnobryum Alopecurum Nieuwland Ex Gangulee (Bryophyta, Neckeraceae) in In Vitro Conditions",
number = "1",
volume = "44",
pages = "344",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1210"
}

Sabovljević, A., Vujičić, M., Skorić, M., Bajić-Ljubicić, J.,& Sabovljević, M. S.. (2012). Axenically Culturing the Bryophytes: Establishment and Propagation of the Pleurocarpous Moss Thamnobryum Alopecurum Nieuwland Ex Gangulee (Bryophyta, Neckeraceae) in In Vitro Conditions. in Pakistan Journal of Botany, 44(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1210

Sabovljević A, Vujičić M, Skorić M, Bajić-Ljubicić J, Sabovljević MS. Axenically Culturing the Bryophytes: Establishment and Propagation of the Pleurocarpous Moss Thamnobryum Alopecurum Nieuwland Ex Gangulee (Bryophyta, Neckeraceae) in In Vitro Conditions. in Pakistan Journal of Botany. 2012;44(1):null-344.
https://hdl.handle.net/21.15107/rcub_ibiss_1210 .

Sabovljević, Aneta, Vujičić, Milorad, Skorić, Marijana, Bajić-Ljubicić, Jasna, Sabovljević, Marko S, "Axenically Culturing the Bryophytes: Establishment and Propagation of the Pleurocarpous Moss Thamnobryum Alopecurum Nieuwland Ex Gangulee (Bryophyta, Neckeraceae) in In Vitro Conditions" in Pakistan Journal of Botany, 44, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1210 .

TY  - JOUR
AU  - Sabovljević, Aneta
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Vujičić, Milorad
AU  - Pejin, Boris
AU  - Sabovljević, Marko S
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1305
AB  - The antifungal activity of dimethyl sulfoxide (DMSO) extracts of three bryophyte species, two mosses (Atrichum undulatum (Hedw.) P. Beauv., Physcomitrella patens (Hedw.) Bruch and Schimp) and a liverwort (Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier), grown in nature and in axenic culture was evaluated by microdilution method against five fungal species (Aspergillus versicolor, Aspergillus fumigatus, Penicillium funiculosum, Penicillium ochrochloron and Trichoderma viride). All the investigated bryophyte extracts have been proved to be active against all fungi tested. In general, extracts made from material grown in the laboratory (in vitro) conditions express better antifungal activity comparing to those made from material grown in the nature. Some of the fungi tested react similarly to both extracts.
T2  - Journal of Medicinal Plants Research
T1  - Bio-activities of extracts from some axenically farmed and naturally grown bryophytes
IS  - 4
VL  - 5
EP  - 571
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1305
ER  - 

@article{
author = "Sabovljević, Aneta and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Vujičić, Milorad and Pejin, Boris and Sabovljević, Marko S",
year = "2011",
abstract = "The antifungal activity of dimethyl sulfoxide (DMSO) extracts of three bryophyte species, two mosses (Atrichum undulatum (Hedw.) P. Beauv., Physcomitrella patens (Hedw.) Bruch and Schimp) and a liverwort (Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier), grown in nature and in axenic culture was evaluated by microdilution method against five fungal species (Aspergillus versicolor, Aspergillus fumigatus, Penicillium funiculosum, Penicillium ochrochloron and Trichoderma viride). All the investigated bryophyte extracts have been proved to be active against all fungi tested. In general, extracts made from material grown in the laboratory (in vitro) conditions express better antifungal activity comparing to those made from material grown in the nature. Some of the fungi tested react similarly to both extracts.",
journal = "Journal of Medicinal Plants Research",
title = "Bio-activities of extracts from some axenically farmed and naturally grown bryophytes",
number = "4",
volume = "5",
pages = "571",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1305"
}

Sabovljević, A., Soković, M., Glamočlija, J., Ćirić, A., Vujičić, M., Pejin, B.,& Sabovljević, M. S.. (2011). Bio-activities of extracts from some axenically farmed and naturally grown bryophytes. in Journal of Medicinal Plants Research, 5(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1305

Sabovljević A, Soković M, Glamočlija J, Ćirić A, Vujičić M, Pejin B, Sabovljević MS. Bio-activities of extracts from some axenically farmed and naturally grown bryophytes. in Journal of Medicinal Plants Research. 2011;5(4):null-571.
https://hdl.handle.net/21.15107/rcub_ibiss_1305 .

Sabovljević, Aneta, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Vujičić, Milorad, Pejin, Boris, Sabovljević, Marko S, "Bio-activities of extracts from some axenically farmed and naturally grown bryophytes" in Journal of Medicinal Plants Research, 5, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1305 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Ilić, Milena D
AU  - Živković, Suzana
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
AU  - Sabovljević, Marko S
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1320
AB  - Salt demonstrates various osmotic and ionic effects on vascular plant growth, development and function, but very few data can be found on how salt affects non-tracheophytes. To explore this, gametophytes of two moss - Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv., and three fern species - Asplenium viride Britton, Ceterach officinarum DC, and Phyllitis scolopendrium (L.) Newman, were treated for 3 days with different NaCl concentrations in growth medium under in vitro controlled conditions. Subsequently, these plants recovered for 18 days on NaCl-free medium, after which the following parameters were measured for mosses: presence of secondary protonema and shoots, protonemal radius and index of multiplication. Survival, chlorophyll a, b, total and a/b ratio were determined as well as total phenolic content, both for ferns and mosses. All species tolerated 50 and 100mMof NaCl-enriched media, quite well. On higher salt concentrations in the substrata, measured morphological parameters and chlorophyll content were reduced. In general, mosses exhibited higher NaCl tolerance than ferns. Change of phenolic content in ferns suggests these plants use antioxidative properties of phenolics as a mechanism of salt tolerance, in contrast with mosses whose phenolic content was stable.
T2  - Australian Journal of Botany
T1  - Comparative study on the effects of NaCl on selected moss and fern representatives
IS  - 8
VL  - 59
EP  - 740
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1320
ER  - 

@article{
author = "Bogdanović, Milica and Ilić, Milena D and Živković, Suzana and Sabovljević, Aneta and Grubišić, Dragoljub and Sabovljević, Marko S",
year = "2011",
abstract = "Salt demonstrates various osmotic and ionic effects on vascular plant growth, development and function, but very few data can be found on how salt affects non-tracheophytes. To explore this, gametophytes of two moss - Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv., and three fern species - Asplenium viride Britton, Ceterach officinarum DC, and Phyllitis scolopendrium (L.) Newman, were treated for 3 days with different NaCl concentrations in growth medium under in vitro controlled conditions. Subsequently, these plants recovered for 18 days on NaCl-free medium, after which the following parameters were measured for mosses: presence of secondary protonema and shoots, protonemal radius and index of multiplication. Survival, chlorophyll a, b, total and a/b ratio were determined as well as total phenolic content, both for ferns and mosses. All species tolerated 50 and 100mMof NaCl-enriched media, quite well. On higher salt concentrations in the substrata, measured morphological parameters and chlorophyll content were reduced. In general, mosses exhibited higher NaCl tolerance than ferns. Change of phenolic content in ferns suggests these plants use antioxidative properties of phenolics as a mechanism of salt tolerance, in contrast with mosses whose phenolic content was stable.",
journal = "Australian Journal of Botany",
title = "Comparative study on the effects of NaCl on selected moss and fern representatives",
number = "8",
volume = "59",
pages = "740",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1320"
}

Bogdanović, M., Ilić, M. D., Živković, S., Sabovljević, A., Grubišić, D.,& Sabovljević, M. S.. (2011). Comparative study on the effects of NaCl on selected moss and fern representatives. in Australian Journal of Botany, 59(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1320

Bogdanović M, Ilić MD, Živković S, Sabovljević A, Grubišić D, Sabovljević MS. Comparative study on the effects of NaCl on selected moss and fern representatives. in Australian Journal of Botany. 2011;59(8):null-740.
https://hdl.handle.net/21.15107/rcub_ibiss_1320 .

Bogdanović, Milica, Ilić, Milena D, Živković, Suzana, Sabovljević, Aneta, Grubišić, Dragoljub, Sabovljević, Marko S, "Comparative study on the effects of NaCl on selected moss and fern representatives" in Australian Journal of Botany, 59, no. 8 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1320 .

TY  - JOUR
AU  - Sabovljević, Aneta
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Vujičić, Milorad
AU  - Pejin, Boris
AU  - Sabovljević, Marko S
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1377
AB  - The antimicrobial activity of DMSO extracts of three bryophyte species, two mosses and a liverwort (Atrichum undulatum (Hedw.) P. Beauv., Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier, Physcomitrella patens (Hedw.) Bruch and Schimp.) grown in nature and in axenic culture was evaluated by microdilution method against eight bacterial species (Escherichia coli ATCC 35210, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Enterobacter cloacae (human isolate), Listeria monocytogenes NCTC 7973, Bacillus cereus (human isolate), Micrococcus flavus ATCC 10240 and Staphilococcus aureus ATCC 6538). All investigated bryophyte extracts are proved to be active against all bacteria tested. In general, extracts made from material grown in nature express better anti-bacterial activity comparing to those made from material grown in laboratory conditions. Some of the bacteria react the same to both extracts and some even better to the extracts made from axenically grown material.
T2  - African Journal of Microbiology Research
T1  - Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species
IS  - 9
VL  - 4
EP  - 812
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1377
ER  - 

@article{
author = "Sabovljević, Aneta and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Vujičić, Milorad and Pejin, Boris and Sabovljević, Marko S",
year = "2010",
abstract = "The antimicrobial activity of DMSO extracts of three bryophyte species, two mosses and a liverwort (Atrichum undulatum (Hedw.) P. Beauv., Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier, Physcomitrella patens (Hedw.) Bruch and Schimp.) grown in nature and in axenic culture was evaluated by microdilution method against eight bacterial species (Escherichia coli ATCC 35210, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Enterobacter cloacae (human isolate), Listeria monocytogenes NCTC 7973, Bacillus cereus (human isolate), Micrococcus flavus ATCC 10240 and Staphilococcus aureus ATCC 6538). All investigated bryophyte extracts are proved to be active against all bacteria tested. In general, extracts made from material grown in nature express better anti-bacterial activity comparing to those made from material grown in laboratory conditions. Some of the bacteria react the same to both extracts and some even better to the extracts made from axenically grown material.",
journal = "African Journal of Microbiology Research",
title = "Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species",
number = "9",
volume = "4",
pages = "812",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1377"
}

Sabovljević, A., Soković, M., Glamočlija, J., Ćirić, A., Vujičić, M., Pejin, B.,& Sabovljević, M. S.. (2010). Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species. in African Journal of Microbiology Research, 4(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1377

Sabovljević A, Soković M, Glamočlija J, Ćirić A, Vujičić M, Pejin B, Sabovljević MS. Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species. in African Journal of Microbiology Research. 2010;4(9):null-812.
https://hdl.handle.net/21.15107/rcub_ibiss_1377 .

Sabovljević, Aneta, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Vujičić, Milorad, Pejin, Boris, Sabovljević, Marko S, "Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species" in African Journal of Microbiology Research, 4, no. 9 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1377 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Sabovljević, Marko
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://botanicaserbica.bio.bg.ac.rs/2009_33_1.html#a488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6175
AB  - In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?
IS  - 1
VL  - 33
SP  - 75
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6175
ER  - 

@article{
author = "Bogdanović, Milica and Sabovljević, Marko and Sabovljević, Aneta and Grubišić, Dragoljub",
year = "2009",
abstract = "In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?",
number = "1",
volume = "33",
pages = "75-82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6175"
}

Bogdanović, M., Sabovljević, M., Sabovljević, A.,& Grubišić, D.. (2009). The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 33(1), 75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175

Bogdanović M, Sabovljević M, Sabovljević A, Grubišić D. The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica. 2009;33(1):75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

Bogdanović, Milica, Sabovljević, Marko, Sabovljević, Aneta, Grubišić, Dragoljub, "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?" in Botanica Serbica, 33, no. 1 (2009):75-82,
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Dragićević, Milan
AU  - Platiša, Jelena
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6173
AB  - Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
T1  - Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6173
ER  - 

@conference{
author = "Bogdanović, Milica and Sabovljević, Aneta and Sabovljević, Marko and Dragićević, Milan and Platiša, Jelena and Grubišić, Dragoljub",
year = "2009",
abstract = "Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia",
title = "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6173"
}

Bogdanović, M., Sabovljević, A., Sabovljević, M., Dragićević, M., Platiša, J.,& Grubišić, D.. (2009). Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173

Bogdanović M, Sabovljević A, Sabovljević M, Dragićević M, Platiša J, Grubišić D. Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. 2009;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

Bogdanović, Milica, Sabovljević, Aneta, Sabovljević, Marko, Dragićević, Milan, Platiša, Jelena, Grubišić, Dragoljub, "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L." in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia (2009):87,
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

TY  - CONF
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
AU  - Sabovljević, Marko S
AU  - Konjević, Radomir M
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1744
C3  - Acta Physiologiae Plantarum
T1  - Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro
IS  - 3
VL  - 26
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1744
ER  - 

@conference{
author = "Sabovljević, Aneta and Grubišić, Dragoljub and Sabovljević, Marko S and Konjević, Radomir M",
year = "2004",
journal = "Acta Physiologiae Plantarum",
title = "Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro",
number = "3",
volume = "26",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1744"
}

Sabovljević, A., Grubišić, D., Sabovljević, M. S.,& Konjević, R. M.. (2004). Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro. in Acta Physiologiae Plantarum, 26(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1744

Sabovljević A, Grubišić D, Sabovljević MS, Konjević RM. Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro. in Acta Physiologiae Plantarum. 2004;26(3):null-53.
https://hdl.handle.net/21.15107/rcub_ibiss_1744 .

Sabovljević, Aneta, Grubišić, Dragoljub, Sabovljević, Marko S, Konjević, Radomir M, "Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro" in Acta Physiologiae Plantarum, 26, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1744 .